ATE443071T1 - Verfahren zum entschützen von oligonukleotiden - Google Patents

Verfahren zum entschützen von oligonukleotiden

Info

Publication number
ATE443071T1
ATE443071T1 AT00907140T AT00907140T ATE443071T1 AT E443071 T1 ATE443071 T1 AT E443071T1 AT 00907140 T AT00907140 T AT 00907140T AT 00907140 T AT00907140 T AT 00907140T AT E443071 T1 ATE443071 T1 AT E443071T1
Authority
AT
Austria
Prior art keywords
oligonucleotide
substrate
protecting groups
phosphate protecting
attached
Prior art date
Application number
AT00907140T
Other languages
German (de)
English (en)
Inventor
Anila Bhan
Lars Holmberg
Original Assignee
Ge Healthcare Bio Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ge Healthcare Bio Sciences filed Critical Ge Healthcare Bio Sciences
Application granted granted Critical
Publication of ATE443071T1 publication Critical patent/ATE443071T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT00907140T 1999-02-05 2000-02-05 Verfahren zum entschützen von oligonukleotiden ATE443071T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11857599P 1999-02-05 1999-02-05
PCT/US2000/002845 WO2000046231A1 (en) 1999-02-05 2000-02-05 Method for deprotecting oligonucleotides

Publications (1)

Publication Number Publication Date
ATE443071T1 true ATE443071T1 (de) 2009-10-15

Family

ID=22379444

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00907140T ATE443071T1 (de) 1999-02-05 2000-02-05 Verfahren zum entschützen von oligonukleotiden

Country Status (10)

Country Link
US (1) US6887990B1 (https=)
EP (1) EP1149109B1 (https=)
JP (1) JP4705716B2 (https=)
AT (1) ATE443071T1 (https=)
AU (1) AU767509B2 (https=)
CA (1) CA2361079C (https=)
DE (1) DE60042962D1 (https=)
DK (1) DK1149109T3 (https=)
NO (1) NO20013804L (https=)
WO (1) WO2000046231A1 (https=)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4503828B2 (ja) 1998-02-11 2010-07-14 ユニバーシティー オブ ヒューストン 光生成試薬を用いる化学反応および生化学反応のための方法および装置
US6465628B1 (en) 1999-02-04 2002-10-15 Isis Pharmaceuticals, Inc. Process for the synthesis of oligomeric compounds
WO2002046205A2 (en) 2000-12-05 2002-06-13 Avecia Limited Process for the preparation of phosphorothionate oligonucleotides
US6768005B2 (en) 2000-12-20 2004-07-27 Avecia Limited Process
JP2004521628A (ja) 2001-03-08 2004-07-22 アプレラ コーポレイション オリゴヌクレオチドの切断および脱保護のための試薬
US7211654B2 (en) 2001-03-14 2007-05-01 Regents Of The University Of Michigan Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports
US20030153741A1 (en) * 2002-02-08 2003-08-14 Marek Kwiatkowski Methods for separating oligonucleotides
US8138330B2 (en) * 2006-09-11 2012-03-20 Sigma-Aldrich Co. Llc Process for the synthesis of oligonucleotides
US8513404B2 (en) * 2008-04-24 2013-08-20 Nitto Denko Avecia, Inc. Process for the manufacture of oligonucleotides
WO2021045141A1 (ja) * 2019-09-04 2021-03-11 神戸天然物化学株式会社 オリゴヌクレオチドの脱保護方法
US12509483B2 (en) 2019-10-11 2025-12-30 Sumitomo Chemical Company, Limited Method for producing nucleic acid oligomers
WO2021153770A1 (en) 2020-01-29 2021-08-05 Sumitomo Chemical Company, Limited Process of preparing nucleic acid oligomer
US20230312635A1 (en) 2020-01-29 2023-10-05 Sumitomo Chemical Company, Limited Method for producing nucleic acid oligomer
US12522629B2 (en) 2020-03-27 2026-01-13 Sumitomo Chemical Company, Limited Method for producing nucleic acid oligomer
US20230242570A1 (en) 2020-07-09 2023-08-03 Sumitomo Chemical Company, Limited Method for producing nucleic acid oligomer
JP7719788B2 (ja) 2020-09-24 2025-08-06 住友化学株式会社 核酸オリゴマーの製造方法
KR20240082343A (ko) 2021-09-28 2024-06-10 스미또모 가가꾸 가부시끼가이샤 정제 디클로로아세트산의 제조 방법
EP4201947A1 (en) 2021-12-22 2023-06-28 F. Hoffmann-La Roche AG Process for the backbone deprotection of oligonucleotides containing a terminal alkyl phosphonate group
CN114573652B (zh) * 2022-03-15 2025-02-07 安徽瑞拜药业有限公司 药用核酸合成工艺
KR20240162536A (ko) 2022-03-23 2024-11-15 스미또모 가가꾸 가부시끼가이샤 핵산 올리고머의 제조 방법
EP4559925A1 (en) 2022-07-22 2025-05-28 Sumitomo Chemical Company, Limited Production method of oligonucleotide
KR20250046255A (ko) 2022-07-28 2025-04-02 스미또모 가가꾸 가부시끼가이샤 티오화 용액
US20250361540A1 (en) * 2022-09-07 2025-11-27 Nippon Shokubai Co., Ltd. Nucleic acid production method, method for removing impurities, and nucleic acid having less impurities
JP7825064B2 (ja) 2022-10-27 2026-03-05 住友化学株式会社 オリゴヌクレオチドの製造方法
US12415172B2 (en) 2022-12-22 2025-09-16 Synthego Corporation Systems and method for automated oligonucleotide synthesis
CN120418262A (zh) 2022-12-26 2025-08-01 住友化学株式会社 寡核苷酸的制造方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4426517A (en) * 1981-08-24 1984-01-17 Eli Lilly And Company Process for de-cyanoethylating blocked nucleotides
US4417046A (en) * 1981-08-24 1983-11-22 Eli Lilly And Company Process for isolating oligonucleotide product from a coupling reaction mixture
FR2540122B1 (fr) * 1983-01-27 1985-11-29 Centre Nat Rech Scient Nouveaux composes comportant une sequence d'oligonucleotide liee a un agent d'intercalation, leur procede de synthese et leur application
US5362866A (en) * 1983-09-02 1994-11-08 Molecular Biosystems, Inc. Oligonucleotide polymeric support system with an oxidation cleavable link
DE3481060D1 (de) * 1983-09-02 1990-02-22 Molecular Biosystems Inc Oligonukleotid-polymer-tragsystem.
US4672110A (en) 1983-11-29 1987-06-09 Northwestern University Method of deprotecting polynucleotide trimethylethyl phosphotriesters
JPS6242997A (ja) * 1985-08-19 1987-02-24 Akira Kaji ヌクレオシドアルキルホスホネイトの製造方法
SE9003743D0 (sv) * 1990-11-26 1990-11-26 Pharmacia Ab Method and means for oligonucleotide synthesis
DE19627898A1 (de) * 1996-07-11 1998-01-15 Hoechst Ag Festphasensynthese von Oligonucleotiden
US6127533A (en) * 1997-02-14 2000-10-03 Isis Pharmaceuticals, Inc. 2'-O-aminooxy-modified oligonucleotides
US6465628B1 (en) * 1999-02-04 2002-10-15 Isis Pharmaceuticals, Inc. Process for the synthesis of oligomeric compounds

Also Published As

Publication number Publication date
NO20013804L (no) 2001-08-22
DE60042962D1 (de) 2009-10-29
AU2868700A (en) 2000-08-25
DK1149109T3 (da) 2010-01-11
JP4705716B2 (ja) 2011-06-22
CA2361079C (en) 2010-05-25
AU767509B2 (en) 2003-11-13
EP1149109A1 (en) 2001-10-31
WO2000046231A1 (en) 2000-08-10
CA2361079A1 (en) 2000-08-10
NO20013804D0 (no) 2001-08-03
US6887990B1 (en) 2005-05-03
JP2002536381A (ja) 2002-10-29
EP1149109B1 (en) 2009-09-16

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