JP4657454B2 - オリゴヌクレオチドの伸長の制御方法 - Google Patents

オリゴヌクレオチドの伸長の制御方法 Download PDF

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JP4657454B2
JP4657454B2 JP2000594952A JP2000594952A JP4657454B2 JP 4657454 B2 JP4657454 B2 JP 4657454B2 JP 2000594952 A JP2000594952 A JP 2000594952A JP 2000594952 A JP2000594952 A JP 2000594952A JP 4657454 B2 JP4657454 B2 JP 4657454B2
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primer
extended
sequence
oligonucleotide
modified
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JP2002534986A5 (enExample
JP2002534986A (ja
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ヌーリス・クアン
イェン・ピン・リウ
アーラ・リシャンスキ
マーク・テイラー
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アベンティス・ファーマスーティカルズ・インコーポレイテツド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
JP2000594952A 1999-01-19 1999-12-10 オリゴヌクレオチドの伸長の制御方法 Expired - Fee Related JP4657454B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/233,413 US6200757B1 (en) 1999-01-19 1999-01-19 Method for controlling the extension of an oligonucleotide
US09/233,413 1999-01-19
PCT/US1999/029253 WO2000043544A1 (en) 1999-01-19 1999-12-10 Method for controlling the extension of an oligonucleotide

Publications (3)

Publication Number Publication Date
JP2002534986A JP2002534986A (ja) 2002-10-22
JP2002534986A5 JP2002534986A5 (enExample) 2007-01-25
JP4657454B2 true JP4657454B2 (ja) 2011-03-23

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Family Applications (1)

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JP2000594952A Expired - Fee Related JP4657454B2 (ja) 1999-01-19 1999-12-10 オリゴヌクレオチドの伸長の制御方法

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US (1) US6200757B1 (enExample)
EP (1) EP1147230B1 (enExample)
JP (1) JP4657454B2 (enExample)
AT (1) ATE322557T1 (enExample)
CA (1) CA2358992C (enExample)
DE (1) DE69930765T2 (enExample)
HK (1) HK1042324B (enExample)
WO (1) WO2000043544A1 (enExample)

Families Citing this family (27)

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Publication number Priority date Publication date Assignee Title
US6274353B1 (en) 1999-09-22 2001-08-14 Genecopoeia, Inc. Method and compositions for improved polynucleotide synthesis
US7033763B2 (en) * 2000-02-23 2006-04-25 City Of Hope Pyrophosphorolysis activated polymerization (PAP)
DE60139762D1 (de) * 2000-02-23 2009-10-15 Hope City Pyrophosphorolyse-aktivierte polymerisierung (pap): anwendung für allel-spezifische amplifizierung und sequenzbestimmung von nukleinsäuren
EP1275735A1 (en) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Composition and method for hot start nucleic acid amplification
US6667165B2 (en) 2001-11-13 2003-12-23 Eppendorf Ag Method and compositions for reversible inhibition of thermostable polymerases
US7211382B2 (en) * 2002-04-09 2007-05-01 Orchid Cellmark Inc. Primer extension using modified nucleotides
CA2521127C (en) 2003-04-01 2014-05-27 Eragen Biosciences, Inc. Polymerase inhibitor and method of using same
EP2489746B1 (en) 2005-06-07 2016-02-03 Luminex Corporation Methods for detection and typing of nucleic acids
EP1896602A4 (en) * 2005-06-09 2009-08-12 Epoch Biosciences Inc IMPROVED AMPLIFICATION PROCESSES ON PRIMER BASIS
CA2621146A1 (en) * 2005-09-06 2007-03-15 Gen-Probe Incorporated Methods, compositions and kits for isothermal amplification of nucleic acids
EP2004852B1 (en) * 2006-03-28 2010-12-15 Commonwealth Scientific and Industrial Research Organisation Amplification of dna fragments
DE102008005667A1 (de) 2008-01-19 2009-07-30 Olfert Landt Basenmodifizierte Primeroligomere für die Multiplex-RT-PCR
EP2337865B1 (en) 2008-10-20 2014-11-19 Roche Diagnostics GmbH Allele-specific amplification using a primer with a modified nucleotide
AU2010307991A1 (en) * 2009-10-13 2012-05-10 Syntezza Molecular Detection Israel Ltd. Methods and compositions for amplifying target sequences from nucleic acid samples
US9238832B2 (en) 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
US8614071B2 (en) 2009-12-11 2013-12-24 Roche Molecular Systems, Inc. Preferential amplification of mRNA over DNA using chemically modified primers
ES2908415T3 (es) 2010-06-21 2022-04-29 Life Technologies Corp Composición y método para la síntesis y amplificación de ácidos nucleicos mediante el uso de RT
EP3502272A1 (en) 2010-06-21 2019-06-26 Life Technologies Corporation Compositions, kits, and methods for synthesis and/or detection of nucleic acids
US9115394B2 (en) 2011-12-22 2015-08-25 Roche Molecular Systems, Inc. Methods and reagents for reducing non-specific amplification
ES2905448T3 (es) 2012-05-10 2022-04-08 Massachusetts Gen Hospital Métodos para determinar una secuencia nucleotídica
CN106661612A (zh) 2014-01-27 2017-05-10 通用医疗公司 制备用于测序的核酸的方法
US9909169B2 (en) 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
CA3037185A1 (en) 2016-09-15 2018-03-22 ArcherDX, Inc. Methods of nucleic acid sample preparation
US10683531B2 (en) 2016-09-15 2020-06-16 ArcherDX, Inc. Methods of nucleic acid sample preparation for analysis of cell-free DNA
AU2019276719B2 (en) * 2018-05-31 2025-11-13 Pacific Biosciences Of California, Inc. Increased signal to noise in nucleic acid sequencing
US11180794B2 (en) 2018-05-31 2021-11-23 Omniome, Inc. Methods and compositions for capping nucleic acids
WO2025007089A1 (en) 2023-06-30 2025-01-02 Life Technologies Corporation Compositions, methods and kits for nucleic acids synthesis and amplification

Family Cites Families (17)

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Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5508178A (en) 1989-01-19 1996-04-16 Rose; Samuel Nucleic acid amplification using single primer
CA2008096A1 (en) * 1989-01-19 1990-07-19 Samuel Rose Nucleic acid amplification using single primer
US5043272A (en) 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
KR950013953B1 (ko) 1990-01-26 1995-11-18 애보트 래보라토리즈 리가제 연쇄 반응에 적용가능한 표적 핵산의 증폭 방법
WO1991012342A1 (en) 1990-02-16 1991-08-22 F. Hoffmann-La Roche Ag Improvements in the specificity and convenience of the polymerase chain reaction
US5348853A (en) 1991-12-16 1994-09-20 Biotronics Corporation Method for reducing non-specific priming in DNA amplification
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5565339A (en) 1992-10-08 1996-10-15 Hoffmann-La Roche Inc. Compositions and methods for inhibiting dimerization of primers during storage of polymerase chain reaction reagents
US5599660A (en) 1993-01-19 1997-02-04 Pharmacia Biotech Inc. Method and preparation for sequential delivery of wax-embedded, inactivated biological and chemical reagents
FR2722799B1 (fr) 1994-07-21 1996-10-04 Parteurop Procede d'amplification d'acide nucleique a l'aide d'un nucleoside modifie, et detection du produit d'amplification a l'aide d'anticorps
US5705366A (en) 1994-09-15 1998-01-06 Johnson & Johnson Clinical Diagnostics, Inc. Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor
US6013439A (en) * 1995-12-22 2000-01-11 Dade Behring Marburg Gmbh Detection of differences in nucleic acids
CA2217143A1 (en) * 1996-02-01 1997-08-07 Behringwerke Aktiengesellschaft Positive controls for use in polynucleotide amplification
JP3545158B2 (ja) * 1996-03-14 2004-07-21 シスメックス株式会社 遺伝子検出法
US6482590B1 (en) * 1996-12-20 2002-11-19 Aventis Behring Gmbh Method for polynucleotide amplification
ATE280177T1 (de) * 1997-03-20 2004-11-15 Hoffmann La Roche Modifizierte primer

Also Published As

Publication number Publication date
US6200757B1 (en) 2001-03-13
EP1147230A1 (en) 2001-10-24
WO2000043544A1 (en) 2000-07-27
HK1042324A1 (en) 2002-08-09
DE69930765D1 (de) 2006-05-18
CA2358992A1 (en) 2000-07-27
JP2002534986A (ja) 2002-10-22
EP1147230B1 (en) 2006-04-05
ATE322557T1 (de) 2006-04-15
DE69930765T2 (de) 2006-11-23
HK1042324B (en) 2006-06-23
CA2358992C (en) 2010-10-12

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