JP2002534986A5 - - Google Patents

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Publication number
JP2002534986A5
JP2002534986A5 JP2000594952A JP2000594952A JP2002534986A5 JP 2002534986 A5 JP2002534986 A5 JP 2002534986A5 JP 2000594952 A JP2000594952 A JP 2000594952A JP 2000594952 A JP2000594952 A JP 2000594952A JP 2002534986 A5 JP2002534986 A5 JP 2002534986A5
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JP
Japan
Prior art keywords
primer
extended
sequence
target
oligonucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2000594952A
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English (en)
Japanese (ja)
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JP2002534986A (ja
JP4657454B2 (ja
Filing date
Publication date
Priority claimed from US09/233,413 external-priority patent/US6200757B1/en
Application filed filed Critical
Publication of JP2002534986A publication Critical patent/JP2002534986A/ja
Publication of JP2002534986A5 publication Critical patent/JP2002534986A5/ja
Application granted granted Critical
Publication of JP4657454B2 publication Critical patent/JP4657454B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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JP2000594952A 1999-01-19 1999-12-10 オリゴヌクレオチドの伸長の制御方法 Expired - Fee Related JP4657454B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/233,413 1999-01-19
US09/233,413 US6200757B1 (en) 1999-01-19 1999-01-19 Method for controlling the extension of an oligonucleotide
PCT/US1999/029253 WO2000043544A1 (en) 1999-01-19 1999-12-10 Method for controlling the extension of an oligonucleotide

Publications (3)

Publication Number Publication Date
JP2002534986A JP2002534986A (ja) 2002-10-22
JP2002534986A5 true JP2002534986A5 (enExample) 2007-01-25
JP4657454B2 JP4657454B2 (ja) 2011-03-23

Family

ID=22877144

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2000594952A Expired - Fee Related JP4657454B2 (ja) 1999-01-19 1999-12-10 オリゴヌクレオチドの伸長の制御方法

Country Status (8)

Country Link
US (1) US6200757B1 (enExample)
EP (1) EP1147230B1 (enExample)
JP (1) JP4657454B2 (enExample)
AT (1) ATE322557T1 (enExample)
CA (1) CA2358992C (enExample)
DE (1) DE69930765T2 (enExample)
HK (1) HK1042324B (enExample)
WO (1) WO2000043544A1 (enExample)

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US6274353B1 (en) * 1999-09-22 2001-08-14 Genecopoeia, Inc. Method and compositions for improved polynucleotide synthesis
CA2395391C (en) * 2000-02-23 2011-08-02 City Of Hope Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination
US7033763B2 (en) * 2000-02-23 2006-04-25 City Of Hope Pyrophosphorolysis activated polymerization (PAP)
EP1275735A1 (en) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Composition and method for hot start nucleic acid amplification
US6667165B2 (en) 2001-11-13 2003-12-23 Eppendorf Ag Method and compositions for reversible inhibition of thermostable polymerases
US7211382B2 (en) * 2002-04-09 2007-05-01 Orchid Cellmark Inc. Primer extension using modified nucleotides
CA2521127C (en) 2003-04-01 2014-05-27 Eragen Biosciences, Inc. Polymerase inhibitor and method of using same
CA2610863C (en) * 2005-06-07 2017-01-17 Eragen Biosciences, Inc. Methods for detection and typing of nucleic acids
JP2008543288A (ja) * 2005-06-09 2008-12-04 エポック バイオサイエンシズ インコーポレーティッド プライマーに基づく改善された増幅法
JP2009506788A (ja) * 2005-09-06 2009-02-19 ジェン−プローブ・インコーポレーテッド 核酸の等温増幅のための方法、組成物及びキット
US8501403B2 (en) * 2006-03-28 2013-08-06 Commonwealth Scientific And Industrial Research Organisation Amplification of DNA fragments
DE102008005667A1 (de) 2008-01-19 2009-07-30 Olfert Landt Basenmodifizierte Primeroligomere für die Multiplex-RT-PCR
EP2337865B1 (en) 2008-10-20 2014-11-19 Roche Diagnostics GmbH Allele-specific amplification using a primer with a modified nucleotide
JP2013507910A (ja) * 2009-10-13 2013-03-07 シンテッツァ モレキュラー ディテクション イスラエル リミテッド 核酸試料から標的配列を増幅するための方法および組成物
US8614071B2 (en) 2009-12-11 2013-12-24 Roche Molecular Systems, Inc. Preferential amplification of mRNA over DNA using chemically modified primers
US9238832B2 (en) 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
EP3150723B1 (en) 2010-06-21 2019-01-30 Life Technologies Corporation Compositions, kits, and methods for synthesis and/or detection of nucleic acids
DK2582839T3 (en) 2010-06-21 2016-08-29 Life Technologies Corp Compositions, methods and kits for nucleic acid synthesis and amplification by RT
US9115394B2 (en) 2011-12-22 2015-08-25 Roche Molecular Systems, Inc. Methods and reagents for reducing non-specific amplification
JP6445426B2 (ja) 2012-05-10 2018-12-26 ザ ジェネラル ホスピタル コーポレイション ヌクレオチド配列を決定する方法
CA2938080A1 (en) 2014-01-27 2015-07-30 The General Hospital Corporation Methods of preparing nucleic acids for sequencing
US9909169B2 (en) 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
JP6997773B2 (ja) 2016-09-15 2022-01-18 アーチャーディーエックス， エルエルシー 無細胞ｄｎａの分析用の核酸サンプル調製の方法
US10704082B2 (en) 2016-09-15 2020-07-07 ArcherDX, Inc. Methods of nucleic acid sample preparation
US11180794B2 (en) 2018-05-31 2021-11-23 Omniome, Inc. Methods and compositions for capping nucleic acids
EP3802874A1 (en) * 2018-05-31 2021-04-14 Omniome, Inc. Increased signal to noise in nucleic acid sequencing
WO2025007089A1 (en) 2023-06-30 2025-01-02 Life Technologies Corporation Compositions, methods and kits for nucleic acids synthesis and amplification

Family Cites Families (17)

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Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5508178A (en) 1989-01-19 1996-04-16 Rose; Samuel Nucleic acid amplification using single primer
ATE142272T1 (de) * 1989-01-19 1996-09-15 Behringwerke Ag Nukleinsäure-amplifikation unter verwendung eines einzelprimers
US5043272A (en) 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
ES2089038T3 (es) 1990-01-26 1996-10-01 Abbott Lab Procedimiento mejorado para amplificar acidos nucleicos blanco aplicable para la reaccion en cadena de polimerasa y ligasa.
ES2141088T3 (es) 1990-02-16 2000-03-16 Hoffmann La Roche Mejoras en la especificidad y conveniencia de la reaccion en cadena de la polimerasa.
US5348853A (en) 1991-12-16 1994-09-20 Biotronics Corporation Method for reducing non-specific priming in DNA amplification
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5565339A (en) 1992-10-08 1996-10-15 Hoffmann-La Roche Inc. Compositions and methods for inhibiting dimerization of primers during storage of polymerase chain reaction reagents
US5599660A (en) 1993-01-19 1997-02-04 Pharmacia Biotech Inc. Method and preparation for sequential delivery of wax-embedded, inactivated biological and chemical reagents
FR2722799B1 (fr) * 1994-07-21 1996-10-04 Parteurop Procede d'amplification d'acide nucleique a l'aide d'un nucleoside modifie, et detection du produit d'amplification a l'aide d'anticorps
US5705366A (en) 1994-09-15 1998-01-06 Johnson & Johnson Clinical Diagnostics, Inc. Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor
WO1997023646A1 (en) * 1995-12-22 1997-07-03 Behringwerke Aktiengesellschaft Detection of differences in nucleic acids
WO1997028279A2 (en) * 1996-02-01 1997-08-07 Behringwerke Aktiengesellschaft Positive controls for use in polynucleotide amplification
JP3545158B2 (ja) * 1996-03-14 2004-07-21 シスメックス株式会社 遺伝子検出法
US6482590B1 (en) * 1996-12-20 2002-11-19 Aventis Behring Gmbh Method for polynucleotide amplification
EP0866071B1 (en) * 1997-03-20 2004-10-20 F. Hoffmann-La Roche Ag Modified primers

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