ATE322557T1 - Methode zur kontrollierten verlängerung eines oligonukleotids - Google Patents

Methode zur kontrollierten verlängerung eines oligonukleotids

Info

Publication number
ATE322557T1
ATE322557T1 AT99965197T AT99965197T ATE322557T1 AT E322557 T1 ATE322557 T1 AT E322557T1 AT 99965197 T AT99965197 T AT 99965197T AT 99965197 T AT99965197 T AT 99965197T AT E322557 T1 ATE322557 T1 AT E322557T1
Authority
AT
Austria
Prior art keywords
oligonucleotide
polynucleotide sequence
target polynucleotide
binding substance
extension
Prior art date
Application number
AT99965197T
Other languages
German (de)
English (en)
Inventor
Nurith Kurn
Yen Ping Liu
Alla Lishanski
Marc Taylor
Original Assignee
Aventis Pharma Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pharma Inc filed Critical Aventis Pharma Inc
Application granted granted Critical
Publication of ATE322557T1 publication Critical patent/ATE322557T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT99965197T 1999-01-19 1999-12-10 Methode zur kontrollierten verlängerung eines oligonukleotids ATE322557T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/233,413 US6200757B1 (en) 1999-01-19 1999-01-19 Method for controlling the extension of an oligonucleotide

Publications (1)

Publication Number Publication Date
ATE322557T1 true ATE322557T1 (de) 2006-04-15

Family

ID=22877144

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99965197T ATE322557T1 (de) 1999-01-19 1999-12-10 Methode zur kontrollierten verlängerung eines oligonukleotids

Country Status (8)

Country Link
US (1) US6200757B1 (enExample)
EP (1) EP1147230B1 (enExample)
JP (1) JP4657454B2 (enExample)
AT (1) ATE322557T1 (enExample)
CA (1) CA2358992C (enExample)
DE (1) DE69930765T2 (enExample)
HK (1) HK1042324B (enExample)
WO (1) WO2000043544A1 (enExample)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6274353B1 (en) 1999-09-22 2001-08-14 Genecopoeia, Inc. Method and compositions for improved polynucleotide synthesis
US7033763B2 (en) * 2000-02-23 2006-04-25 City Of Hope Pyrophosphorolysis activated polymerization (PAP)
EP1259646B1 (en) * 2000-02-23 2009-09-02 City of Hope Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination
EP1275735A1 (en) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Composition and method for hot start nucleic acid amplification
US6667165B2 (en) * 2001-11-13 2003-12-23 Eppendorf Ag Method and compositions for reversible inhibition of thermostable polymerases
US7211382B2 (en) * 2002-04-09 2007-05-01 Orchid Cellmark Inc. Primer extension using modified nucleotides
EP1615942B1 (en) 2003-04-01 2016-03-16 Luminex Corporation Polymerase inhibitor and method of using same
EP1893777B1 (en) 2005-06-07 2014-10-01 Luminex Corporation Methods for detection and typing of nucleic acids
EP1896602A4 (en) * 2005-06-09 2009-08-12 Epoch Biosciences Inc IMPROVED AMPLIFICATION PROCESSES ON PRIMER BASIS
US20070054301A1 (en) * 2005-09-06 2007-03-08 Gen-Probe Incorporated Methods, compositions and kits for isothermal amplification of nucleic acids
CN101443458B (zh) * 2006-03-28 2014-04-02 联邦科学及工业研究组织 扩增dna片段
DE102008005667A1 (de) 2008-01-19 2009-07-30 Olfert Landt Basenmodifizierte Primeroligomere für die Multiplex-RT-PCR
ES2527653T3 (es) 2008-10-20 2015-01-28 F. Hoffmann-La Roche Ag Amplificación específica de alelo usando un cebador con un nucleótido modificado
EP2488664A4 (en) * 2009-10-13 2014-04-09 Syntezza Molecular Detection Israel Ltd METHOD AND COMPOSITIONS FOR REINFORCING TARGET SEQUENCES FROM NUCLEIC ACID SAMPLES
US9238832B2 (en) 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
US8614071B2 (en) 2009-12-11 2013-12-24 Roche Molecular Systems, Inc. Preferential amplification of mRNA over DNA using chemically modified primers
EP2582839B1 (en) 2010-06-21 2016-05-18 Life Technologies Corporation Compositions, methods and kits for nucleic acid synthesis and amplification using rt
CN106929506A (zh) 2010-06-21 2017-07-07 生命技术公司 用于核酸的合成和/或检测的组合物、试剂盒及方法
US9115394B2 (en) 2011-12-22 2015-08-25 Roche Molecular Systems, Inc. Methods and reagents for reducing non-specific amplification
ES2905448T3 (es) 2012-05-10 2022-04-08 Massachusetts Gen Hospital Métodos para determinar una secuencia nucleotídica
WO2015112974A1 (en) 2014-01-27 2015-07-30 The General Hospital Corporation Methods of preparing nucleic acids for sequencing
US9909169B2 (en) 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
EP4353830A3 (en) 2016-09-15 2024-07-03 ArcherDX, LLC Methods of nucleic acid sample preparation for analysis of cell-free dna
JP6997772B2 (ja) 2016-09-15 2022-01-18 アーチャーディーエックス， エルエルシー 核酸サンプル調製の方法
US11180794B2 (en) 2018-05-31 2021-11-23 Omniome, Inc. Methods and compositions for capping nucleic acids
JP2021525078A (ja) * 2018-05-31 2021-09-24 オムニオム インコーポレイテッドＯｍｎｉｏｍｅ， Ｉｎｃ． 核酸配列決定におけるシグナル対ノイズの増加
WO2025007089A1 (en) 2023-06-30 2025-01-02 Life Technologies Corporation Compositions, methods and kits for nucleic acids synthesis and amplification

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5508178A (en) 1989-01-19 1996-04-16 Rose; Samuel Nucleic acid amplification using single primer
CA2008096A1 (en) * 1989-01-19 1990-07-19 Samuel Rose Nucleic acid amplification using single primer
US5043272A (en) 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
EP0439182B1 (en) 1990-01-26 1996-04-24 Abbott Laboratories Improved method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
AU653712B2 (en) 1990-02-16 1994-10-13 F. Hoffmann-La Roche Ag Improvements in the specificity and convenience of the polymerase chain reaction
US5348853A (en) 1991-12-16 1994-09-20 Biotronics Corporation Method for reducing non-specific priming in DNA amplification
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5565339A (en) 1992-10-08 1996-10-15 Hoffmann-La Roche Inc. Compositions and methods for inhibiting dimerization of primers during storage of polymerase chain reaction reagents
US5599660A (en) 1993-01-19 1997-02-04 Pharmacia Biotech Inc. Method and preparation for sequential delivery of wax-embedded, inactivated biological and chemical reagents
FR2722799B1 (fr) * 1994-07-21 1996-10-04 Parteurop Procede d'amplification d'acide nucleique a l'aide d'un nucleoside modifie, et detection du produit d'amplification a l'aide d'anticorps
US5705366A (en) 1994-09-15 1998-01-06 Johnson & Johnson Clinical Diagnostics, Inc. Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor
US6013439A (en) * 1995-12-22 2000-01-11 Dade Behring Marburg Gmbh Detection of differences in nucleic acids
DE69714526T2 (de) * 1996-02-01 2003-03-20 Aventis Pharmaceuticals Inc., Bridgewater Positiv kontrollen zur verwendung bei polynukleotid - vervielfältigungen
JP3545158B2 (ja) * 1996-03-14 2004-07-21 シスメックス株式会社 遺伝子検出法
US6482590B1 (en) * 1996-12-20 2002-11-19 Aventis Behring Gmbh Method for polynucleotide amplification
EP0866071B1 (en) * 1997-03-20 2004-10-20 F. Hoffmann-La Roche Ag Modified primers

Also Published As

Publication number Publication date
HK1042324A1 (en) 2002-08-09
CA2358992A1 (en) 2000-07-27
DE69930765D1 (de) 2006-05-18
HK1042324B (en) 2006-06-23
CA2358992C (en) 2010-10-12
DE69930765T2 (de) 2006-11-23
EP1147230A1 (en) 2001-10-24
WO2000043544A1 (en) 2000-07-27
EP1147230B1 (en) 2006-04-05
JP2002534986A (ja) 2002-10-22
US6200757B1 (en) 2001-03-13
JP4657454B2 (ja) 2011-03-23

Similar Documents

Publication Publication Date Title
DE69930765D1 (de) Methode zur kontrollierten verlängerung eines oligonukleotids
DK0912767T3 (da) Fremgangsmåde til sporing og forstærkning af nukleinsyresekvenser ved anvendelse af modificerede oligonukleotider med foröget targetspecifikt TM
WO2002052031A3 (en) Nucleic acid amplification
ATE262595T1 (de) Methoden und zusammensetzungen zur linearen isothermalen amplifikation von polynukleotidsequenzen
DE69034177D1 (de) Verfahren zur Amplifikation von Nukleinsäuresequenzen
ATE545710T1 (de) Verfahren zur vervielfältigung von nukleinsäuren
ATE269908T1 (de) Methode zur sequenzierung von nukleinsäuren
DE60043182D1 (de) Methode zur dns-sequenzierung
DE60005309D1 (de) Methode zur sequenzierung von polynukleotiden
DE69333891D1 (de) Verfahren zur Nukleinsäurenamplifizierung, Zusammensetzung und Kit
ATE220426T1 (de) Verfahren zur direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen und seine anwendung
ATE551349T1 (de) Methode unter verwendung von reversibel blockierten markierungsoligonukleotiden
ATE542917T1 (de) Zusammensetzungen, verfahren und kits zur bestimmung des vorhandenseins von trichomonas vaginalis in einer testprobe
ATE403747T1 (de) Verfahren zur amplifikation von signal- flankierenden sequenzen aus unbekannten, genomischen daten
NO20000538L (no) Forsterkning av spesifisiteten til nukleinsyreamplifisering ved bærer nukleinsyre
WO2005054270A3 (en) Identification and design of sirna molecules
WO2004104229A3 (en) Internal control nucleic acid molecule for nucleic acid amplification systems
DE69815370D1 (de) Fluoreszend-konjugaten von nukleosiden und von nukleotiden, ihrer verfahren zur herstellung und ihrer verwendungen
WO2002040126A3 (en) Methods for identifying nucleotides at defined positions in target nucleic acids using fluorescence polarization
WO2005084277A3 (en) Method of isolating nucleic acid targets
ATE420975T1 (de) Methode zur linearen nicht-selektiven amplifikation von nukleinsäuren
ATE197481T1 (de) Verfahren und reagenzien zur typisierung von hla- genen der klasse i
ATE320507T1 (de) Methoden zur dns vervielfältigung und material dafür
WO2001020024A3 (de) Verfahren zum ermitteln von nuklein- und/oder aminosäuresequenzen
WO2001023620A3 (en) Arrays with modified oligonucleotide and polynucleotide compositions

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties