JP4643023B2 - 細胞溶解剤を使用せずに試料からｄｎａを迅速に効率よく捕捉する方法 - Google Patents

細胞溶解剤を使用せずに試料からｄｎａを迅速に効率よく捕捉する方法 Download PDF

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JP4643023B2
JP4643023B2 JP2000615405A JP2000615405A JP4643023B2 JP 4643023 B2 JP4643023 B2 JP 4643023B2 JP 2000615405 A JP2000615405 A JP 2000615405A JP 2000615405 A JP2000615405 A JP 2000615405A JP 4643023 B2 JP4643023 B2 JP 4643023B2
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nucleic acid
water
weakly basic
sample
polymer
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ティー． ベリー，ロバート
スン，チェンボ
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オルソ−クリニカル ダイアグノスティクス，インコーポレイティド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Organic Chemistry (AREA)
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  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
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  • Molecular Biology (AREA)
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  • Hospice & Palliative Care (AREA)
  • Plant Pathology (AREA)
  • Oncology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
JP2000615405A 1999-05-04 2000-05-01 細胞溶解剤を使用せずに試料からｄｎａを迅速に効率よく捕捉する方法 Expired - Fee Related JP4643023B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13244399P 1999-05-04 1999-05-04
PCT/US2000/011651 WO2000066783A2 (en) 1999-05-04 2000-05-01 Rapid and efficient capture of dna from sample without using cell lysing reagent

Related Child Applications (1)

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JP2010186439A Division JP2011015691A (ja) 1999-05-04 2010-08-23 細胞溶解剤を使用せずに試料からｄｎａを迅速に効率よく捕捉する方法

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JP2004508002A JP2004508002A (ja) 2004-03-18
JP2004508002A5 JP2004508002A5 (https=) 2004-12-24
JP4643023B2 true JP4643023B2 (ja) 2011-03-02

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JP2000615405A Expired - Fee Related JP4643023B2 (ja) 1999-05-04 2000-05-01 細胞溶解剤を使用せずに試料からｄｎａを迅速に効率よく捕捉する方法
JP2010186439A Pending JP2011015691A (ja) 1999-05-04 2010-08-23 細胞溶解剤を使用せずに試料からｄｎａを迅速に効率よく捕捉する方法

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US (2) US7615346B2 (https=)
EP (1) EP1206571B1 (https=)
JP (2) JP4643023B2 (https=)
AT (1) ATE278035T1 (https=)
AU (1) AU4682400A (https=)
CA (1) CA2370122C (https=)
DE (1) DE60014399T2 (https=)
DK (1) DK1206571T3 (https=)
ES (1) ES2228520T3 (https=)
PT (1) PT1206571E (https=)
WO (1) WO2000066783A2 (https=)

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CN113355321A (zh) * 2011-09-26 2021-09-07 凯杰有限公司 用于分离细胞外核酸的快速方法
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Also Published As

Publication number Publication date
PT1206571E (pt) 2004-12-31
US7615346B2 (en) 2009-11-10
WO2000066783A3 (en) 2002-01-03
US20090280498A1 (en) 2009-11-12
EP1206571B1 (en) 2004-09-29
WO2000066783A2 (en) 2000-11-09
DE60014399D1 (de) 2004-11-04
AU4682400A (en) 2000-11-17
DK1206571T3 (da) 2004-12-06
ES2228520T3 (es) 2005-04-16
CA2370122C (en) 2011-04-26
EP1206571A2 (en) 2002-05-22
ATE278035T1 (de) 2004-10-15
DE60014399T2 (de) 2005-12-08
JP2004508002A (ja) 2004-03-18
JP2011015691A (ja) 2011-01-27
US20070243542A1 (en) 2007-10-18
CA2370122A1 (en) 2000-11-09

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