CA2370122C - Rapid and efficient capture of dna from sample without using cell lysing reagent - Google Patents

Rapid and efficient capture of dna from sample without using cell lysing reagent Download PDF

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Publication number
CA2370122C
CA2370122C CA2370122A CA2370122A CA2370122C CA 2370122 C CA2370122 C CA 2370122C CA 2370122 A CA2370122 A CA 2370122A CA 2370122 A CA2370122 A CA 2370122A CA 2370122 C CA2370122 C CA 2370122C
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CA
Canada
Prior art keywords
nucleic acid
sample
water
dna
weakly basic
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Expired - Fee Related
Application number
CA2370122A
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English (en)
French (fr)
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CA2370122A1 (en
Inventor
Robert T. Belly
Jianbo Sun
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Ortho Clinical Diagnostics Inc
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Ortho Clinical Diagnostics Inc
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Publication date
Application filed by Ortho Clinical Diagnostics Inc filed Critical Ortho Clinical Diagnostics Inc
Priority to CA2718662A priority Critical patent/CA2718662A1/en
Publication of CA2370122A1 publication Critical patent/CA2370122A1/en
Application granted granted Critical
Publication of CA2370122C publication Critical patent/CA2370122C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Plant Pathology (AREA)
  • Oncology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
CA2370122A 1999-05-04 2000-05-01 Rapid and efficient capture of dna from sample without using cell lysing reagent Expired - Fee Related CA2370122C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2718662A CA2718662A1 (en) 1999-05-04 2000-05-01 Rapid and efficient capture of dna from sample without using cell lysing reagent

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13244399P 1999-05-04 1999-05-04
US60/132,443 1999-05-04
PCT/US2000/011651 WO2000066783A2 (en) 1999-05-04 2000-05-01 Rapid and efficient capture of dna from sample without using cell lysing reagent

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA2718662A Division CA2718662A1 (en) 1999-05-04 2000-05-01 Rapid and efficient capture of dna from sample without using cell lysing reagent

Publications (2)

Publication Number Publication Date
CA2370122A1 CA2370122A1 (en) 2000-11-09
CA2370122C true CA2370122C (en) 2011-04-26

Family

ID=22454077

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2370122A Expired - Fee Related CA2370122C (en) 1999-05-04 2000-05-01 Rapid and efficient capture of dna from sample without using cell lysing reagent

Country Status (11)

Country Link
US (2) US7615346B2 (https=)
EP (1) EP1206571B1 (https=)
JP (2) JP4643023B2 (https=)
AT (1) ATE278035T1 (https=)
AU (1) AU4682400A (https=)
CA (1) CA2370122C (https=)
DE (1) DE60014399T2 (https=)
DK (1) DK1206571T3 (https=)
ES (1) ES2228520T3 (https=)
PT (1) PT1206571E (https=)
WO (1) WO2000066783A2 (https=)

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US20050136477A1 (en) * 2003-11-17 2005-06-23 Hashem Akhavan-Tafti Methods for isolating nucleic acids from biological and cellular materials
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
JP4819064B2 (ja) * 2005-02-18 2011-11-16 ジェン−プロウブ インコーポレイテッド アルカリショックを取り入れたサンプル調製法
JP2008545418A (ja) * 2005-05-27 2008-12-18 ジョン ウェイン キャンサー インスティチュート 癌の診断、予後診断、および治療のための遊離循環dnaの使用
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
DK3088083T3 (en) 2006-03-24 2018-11-26 Handylab Inc Method of carrying out PCR down a multi-track cartridge
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
WO2008060604A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US8709787B2 (en) 2006-11-14 2014-04-29 Handylab, Inc. Microfluidic cartridge and method of using same
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US8206929B2 (en) * 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
JP2013004258A (ja) * 2011-06-15 2013-01-07 Gigaphoton Inc 極端紫外光生成装置及び極端紫外光の生成方法
ES2769028T3 (es) 2011-04-15 2020-06-24 Becton Dickinson Co Termociclador microfluídico de barrido en tiempo real
ES2605372T3 (es) * 2011-05-31 2017-03-14 Berry Genomics Co., Ltd. Un dispositivo para detectar el número de copias de cromosomas fetales o cromosomas de células tumorales
CN113355321A (zh) * 2011-09-26 2021-09-07 凯杰有限公司 用于分离细胞外核酸的快速方法
CN104040238B (zh) 2011-11-04 2017-06-27 汉迪拉布公司 多核苷酸样品制备装置
CN105378108A (zh) * 2013-03-13 2016-03-02 雅培分子公司 用于分离核酸的系统和方法

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Also Published As

Publication number Publication date
PT1206571E (pt) 2004-12-31
US7615346B2 (en) 2009-11-10
WO2000066783A3 (en) 2002-01-03
US20090280498A1 (en) 2009-11-12
EP1206571B1 (en) 2004-09-29
WO2000066783A2 (en) 2000-11-09
DE60014399D1 (de) 2004-11-04
JP4643023B2 (ja) 2011-03-02
AU4682400A (en) 2000-11-17
DK1206571T3 (da) 2004-12-06
ES2228520T3 (es) 2005-04-16
EP1206571A2 (en) 2002-05-22
ATE278035T1 (de) 2004-10-15
DE60014399T2 (de) 2005-12-08
JP2004508002A (ja) 2004-03-18
JP2011015691A (ja) 2011-01-27
US20070243542A1 (en) 2007-10-18
CA2370122A1 (en) 2000-11-09

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