DE60014399D1 - Schnelle und wirksame dns-immobilisierung aus einer probe ohne die verwendung von zelllysereagenzien - Google Patents

Schnelle und wirksame dns-immobilisierung aus einer probe ohne die verwendung von zelllysereagenzien

Info

Publication number
DE60014399D1
DE60014399D1 DE60014399T DE60014399T DE60014399D1 DE 60014399 D1 DE60014399 D1 DE 60014399D1 DE 60014399 T DE60014399 T DE 60014399T DE 60014399 T DE60014399 T DE 60014399T DE 60014399 D1 DE60014399 D1 DE 60014399D1
Authority
DE
Germany
Prior art keywords
nucleic acids
sample
celllysis
agents
fast
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60014399T
Other languages
English (en)
Other versions
DE60014399T2 (de
Inventor
Robert T Belly
Jianbo Sun
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ortho Clinical Diagnostics Inc
Original Assignee
Ortho Clinical Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ortho Clinical Diagnostics Inc filed Critical Ortho Clinical Diagnostics Inc
Application granted granted Critical
Publication of DE60014399D1 publication Critical patent/DE60014399D1/de
Publication of DE60014399T2 publication Critical patent/DE60014399T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
DE60014399T 1999-05-04 2000-05-01 Schnelle und wirksame dns-immobilisierung aus einer probe ohne die verwendung von zelllysereagenzien Expired - Lifetime DE60014399T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13244399P 1999-05-04 1999-05-04
US132443P 1999-05-04
PCT/US2000/011651 WO2000066783A2 (en) 1999-05-04 2000-05-01 Rapid and efficient capture of dna from sample without using cell lysing reagent

Publications (2)

Publication Number Publication Date
DE60014399D1 true DE60014399D1 (de) 2004-11-04
DE60014399T2 DE60014399T2 (de) 2005-12-08

Family

ID=22454077

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60014399T Expired - Lifetime DE60014399T2 (de) 1999-05-04 2000-05-01 Schnelle und wirksame dns-immobilisierung aus einer probe ohne die verwendung von zelllysereagenzien

Country Status (11)

Country Link
US (2) US7615346B2 (de)
EP (1) EP1206571B1 (de)
JP (2) JP4643023B2 (de)
AT (1) ATE278035T1 (de)
AU (1) AU4682400A (de)
CA (1) CA2370122C (de)
DE (1) DE60014399T2 (de)
DK (1) DK1206571T3 (de)
ES (1) ES2228520T3 (de)
PT (1) PT1206571E (de)
WO (1) WO2000066783A2 (de)

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EP2243832A3 (de) * 2005-02-18 2011-01-05 Gen-Probe Incorporated Probenherstellungsverfahren mit einem Alkalistoß
EP1888786A4 (de) * 2005-05-27 2009-12-30 Wayne John Cancer Inst Verwendung freier zirkulierender dna zur diagnose, prognose und behandlung von krebs
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US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
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US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US8206929B2 (en) 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
JP2013004258A (ja) * 2011-06-15 2013-01-07 Gigaphoton Inc 極端紫外光生成装置及び極端紫外光の生成方法
BR112013026451B1 (pt) 2011-04-15 2021-02-09 Becton, Dickinson And Company sistema e método para realizar ensaios de diagnóstico molecular em várias amostras em paralelo e simultaneamente amplificação em tempo real em pluralidade de câmaras de reação de amplificação
CN103080336B (zh) * 2011-05-31 2014-06-04 北京贝瑞和康生物技术有限公司 检测胚胎或肿瘤染色体拷贝数的试剂盒、装置和方法
US10184145B2 (en) * 2011-09-26 2019-01-22 Qiagen Gmbh Rapid method for isolating extracellular nucleic acids
EP2773892B1 (de) 2011-11-04 2020-10-07 Handylab, Inc. Vorrichtung zur vorbereitung von polynukleotidproben
CN105378108A (zh) * 2013-03-13 2016-03-02 雅培分子公司 用于分离核酸的系统和方法

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Also Published As

Publication number Publication date
ES2228520T3 (es) 2005-04-16
JP4643023B2 (ja) 2011-03-02
DE60014399T2 (de) 2005-12-08
CA2370122C (en) 2011-04-26
JP2011015691A (ja) 2011-01-27
WO2000066783A3 (en) 2002-01-03
PT1206571E (pt) 2004-12-31
US7615346B2 (en) 2009-11-10
DK1206571T3 (da) 2004-12-06
WO2000066783A2 (en) 2000-11-09
JP2004508002A (ja) 2004-03-18
US20070243542A1 (en) 2007-10-18
US20090280498A1 (en) 2009-11-12
CA2370122A1 (en) 2000-11-09
AU4682400A (en) 2000-11-17
ATE278035T1 (de) 2004-10-15
EP1206571B1 (de) 2004-09-29
EP1206571A2 (de) 2002-05-22

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Representative=s name: BOEHMERT & BOEHMERT, 28209 BREMEN