AU4682400A - Rapid and efficient capture of dna from sample without using cell lysing reagent - Google Patents

Rapid and efficient capture of dna from sample without using cell lysing reagent

Info

Publication number
AU4682400A
AU4682400A AU46824/00A AU4682400A AU4682400A AU 4682400 A AU4682400 A AU 4682400A AU 46824/00 A AU46824/00 A AU 46824/00A AU 4682400 A AU4682400 A AU 4682400A AU 4682400 A AU4682400 A AU 4682400A
Authority
AU
Australia
Prior art keywords
nucleic acids
sample
cell lysing
rapid
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU46824/00A
Other languages
English (en)
Inventor
Robert T. Belly
Jianbo Sun
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ortho Clinical Diagnostics Inc
Original Assignee
Ortho Clinical Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ortho Clinical Diagnostics Inc filed Critical Ortho Clinical Diagnostics Inc
Publication of AU4682400A publication Critical patent/AU4682400A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Plant Pathology (AREA)
  • Oncology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
AU46824/00A 1999-05-04 2000-05-01 Rapid and efficient capture of dna from sample without using cell lysing reagent Abandoned AU4682400A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13244399P 1999-05-04 1999-05-04
US60132443 1999-05-04
PCT/US2000/011651 WO2000066783A2 (en) 1999-05-04 2000-05-01 Rapid and efficient capture of dna from sample without using cell lysing reagent

Publications (1)

Publication Number Publication Date
AU4682400A true AU4682400A (en) 2000-11-17

Family

ID=22454077

Family Applications (1)

Application Number Title Priority Date Filing Date
AU46824/00A Abandoned AU4682400A (en) 1999-05-04 2000-05-01 Rapid and efficient capture of dna from sample without using cell lysing reagent

Country Status (11)

Country Link
US (2) US7615346B2 (https=)
EP (1) EP1206571B1 (https=)
JP (2) JP4643023B2 (https=)
AT (1) ATE278035T1 (https=)
AU (1) AU4682400A (https=)
CA (1) CA2370122C (https=)
DE (1) DE60014399T2 (https=)
DK (1) DK1206571T3 (https=)
ES (1) ES2228520T3 (https=)
PT (1) PT1206571E (https=)
WO (1) WO2000066783A2 (https=)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005011867A2 (en) 2003-07-31 2005-02-10 Handylab, Inc. Processing particle-containing samples
US20050106602A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Simplified methods for isolating nucleic acids from cellular materials
US20050136477A1 (en) * 2003-11-17 2005-06-23 Hashem Akhavan-Tafti Methods for isolating nucleic acids from biological and cellular materials
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
JP4819064B2 (ja) * 2005-02-18 2011-11-16 ジェン−プロウブ インコーポレイテッド アルカリショックを取り入れたサンプル調製法
JP2008545418A (ja) * 2005-05-27 2008-12-18 ジョン ウェイン キャンサー インスティチュート 癌の診断、予後診断、および治療のための遊離循環ｄｎａの使用
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
DK3088083T3 (en) 2006-03-24 2018-11-26 Handylab Inc Method of carrying out PCR down a multi-track cartridge
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
WO2008060604A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US8709787B2 (en) 2006-11-14 2014-04-29 Handylab, Inc. Microfluidic cartridge and method of using same
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US8206929B2 (en) * 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
JP2013004258A (ja) * 2011-06-15 2013-01-07 Gigaphoton Inc 極端紫外光生成装置及び極端紫外光の生成方法
ES2769028T3 (es) 2011-04-15 2020-06-24 Becton Dickinson Co Termociclador microfluídico de barrido en tiempo real
ES2605372T3 (es) * 2011-05-31 2017-03-14 Berry Genomics Co., Ltd. Un dispositivo para detectar el número de copias de cromosomas fetales o cromosomas de células tumorales
CN113355321A (zh) * 2011-09-26 2021-09-07 凯杰有限公司 用于分离细胞外核酸的快速方法
CN104040238B (zh) 2011-11-04 2017-06-27 汉迪拉布公司 多核苷酸样品制备装置
CN105378108A (zh) * 2013-03-13 2016-03-02 雅培分子公司 用于分离核酸的系统和方法

Family Cites Families (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4089747A (en) 1976-08-09 1978-05-16 Eastman Kodak Company Compositions for the detection of hydrogen peroxide
CA1180647A (en) 1981-07-17 1985-01-08 Cavit Akin Light-emitting polynucleotide hybridization diagnostic method
US4994373A (en) 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
US4713326A (en) 1983-07-05 1987-12-15 Molecular Diagnostics, Inc. Coupling of nucleic acids to solid support by photochemical methods
US4920061A (en) 1984-03-02 1990-04-24 The University Of Texas System Biological magnetic colloids
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4795698A (en) 1985-10-04 1989-01-03 Immunicon Corporation Magnetic-polymer particles
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US4997772A (en) 1987-09-18 1991-03-05 Eastman Kodak Company Water-insoluble particle and immunoreactive reagent, analytical elements and methods of use
JPH0736015B2 (ja) 1987-09-18 1995-04-19 イーストマン コダック カンパニー 診断試験キットおよびリガンドの測定方法
US4914210A (en) 1987-10-02 1990-04-03 Cetus Corporation Oligonucleotide functionalizing reagents
US4962029A (en) 1987-10-02 1990-10-09 Cetus Corporation Covalent oligonucleotide-horseradish peroxidase conjugate
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
IL88923A (en) 1988-01-12 1995-07-31 Hoffmann La Roche Gene encoding a thermostable dna polymerase from thermus aquaticus said dna polymerase and its purification
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
WO1990008840A1 (en) 1989-02-03 1990-08-09 Eastman Kodak Company Nucleic acid test article and its use to detect a predetermined nucleic acid
US5229297A (en) 1989-02-03 1993-07-20 Eastman Kodak Company Containment cuvette for PCR and method of use
US5334499A (en) 1989-04-17 1994-08-02 Eastman Kodak Company Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction
US5231015A (en) 1989-10-18 1993-07-27 Eastman Kodak Company Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme
CA2031659A1 (en) 1990-01-26 1991-07-27 John B. Findlay Water-insoluble reagent, nucleic acid probe, test kit and diagnostic and purification methods
CA2035010C (en) 1990-01-26 1996-12-10 Keith C. Backman Method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
DK0515506T3 (da) 1990-02-16 2000-05-08 Hoffmann La Roche Fremgangsmåde til påvisning af carcinogene humane papillomavirus
US5155166A (en) 1990-06-18 1992-10-13 Eastman Kodak Company Use of 1-(1-pyrrolidinylcarbonyl)pyridinium salts to attach compounds to carboxylated particles and a kit containing same
US5147777A (en) 1990-06-18 1992-09-15 Eastman Kodak Company Biologically active reagents prepared from carboxy-containing polymer, analytical element and methods of use
US5173260A (en) 1990-09-17 1992-12-22 Eastman Kodak Company Beads fused to a test device support
DE69128520T2 (de) 1990-10-31 1998-07-09 Tosoh Corp Verfahren zum Nachweis oder Quantifizierung von Zielnukleinsäuren
US5380489A (en) 1992-02-18 1995-01-10 Eastman Kodak Company Element and method for nucleic acid amplification and detection using adhered probes
JPH0830704B2 (ja) 1991-03-21 1996-03-27 イーストマン コダック カンパニー 核酸増幅のための要素および方法ならびに付着プローブを使用する検出方法
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
AU3919293A (en) * 1992-03-27 1993-11-08 University Of Maryland At Baltimore Detection of gene mutations with mismatch repair enzymes
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
CH686982A5 (fr) * 1993-12-16 1996-08-15 Maurice Stroun Méthode pour le diagnostic de cancers.
US5622822A (en) 1994-09-13 1997-04-22 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same
US5434270A (en) 1994-09-15 1995-07-18 Eastman Kodak Company Weakly basic polymerizable monomers and polymers prepared therefrom
US5582988A (en) * 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
GB9422814D0 (en) * 1994-11-11 1995-01-04 Medinnova Sf Chemical method
AUPN245295A0 (en) * 1995-04-13 1995-05-11 Johnson & Johnson Research Pty. Limited Assay for genetic abnormalities
JP3689896B2 (ja) * 1995-12-04 2005-08-31 Ｊｓｒ株式会社 核酸吸着剤
JP3689897B2 (ja) * 1995-12-04 2005-08-31 Ｊｓｒ株式会社 核酸吸着剤
US5702884A (en) 1996-03-12 1997-12-30 Johnson & Johnson Clinical Diagnostics, Inc. Whole blood sample preparation for polymerase chain reaction using ammonium chloride and a carboxylic acid or metal carboxylate for selective red blood cell lysis
AU2324997A (en) * 1996-03-15 1997-10-01 Penn State Research Foundation, The Detection of extracellular tumor-associated nucleic acid in blood plasma or ser um using nucleic acid amplification assays
GB9715034D0 (en) * 1997-07-18 1997-09-24 Zeneca Ltd Assay
EP2267127A1 (en) 1998-04-28 2010-12-29 Ortho-Clinical Diagnostics, Inc. Improved methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues
AUPQ495700A0 (en) 2000-01-05 2000-02-03 Johnson & Johnson Research Pty. Limited Method for concurrent amplification and real time detection of polymorphic nucleic acid sequences
US7262006B1 (en) * 2000-05-01 2007-08-28 Ortho-Clinical Diagnostics, Inc. Rapid and efficient capture of DNA from sample without using cell lysing reagent

Also Published As

Publication number Publication date
PT1206571E (pt) 2004-12-31
US7615346B2 (en) 2009-11-10
WO2000066783A3 (en) 2002-01-03
US20090280498A1 (en) 2009-11-12
EP1206571B1 (en) 2004-09-29
WO2000066783A2 (en) 2000-11-09
DE60014399D1 (de) 2004-11-04
JP4643023B2 (ja) 2011-03-02
DK1206571T3 (da) 2004-12-06
ES2228520T3 (es) 2005-04-16
CA2370122C (en) 2011-04-26
EP1206571A2 (en) 2002-05-22
ATE278035T1 (de) 2004-10-15
DE60014399T2 (de) 2005-12-08
JP2004508002A (ja) 2004-03-18
JP2011015691A (ja) 2011-01-27
US20070243542A1 (en) 2007-10-18
CA2370122A1 (en) 2000-11-09

Similar Documents

Publication Publication Date Title
AU4682400A (en) Rapid and efficient capture of dna from sample without using cell lysing reagent
EP0707077A3 (en) Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US20110287951A1 (en) Methods and systems for purifying, transferring, and/or manipulating nucleic acids
CN112176035A (zh) 一种新型crispr核酸检测方法及应用
WO2004055205A3 (en) Biological reagents and methods to verify the efficiency of sample preparation and nucleic acid amplification and/or detection
DE19681033D2 (de) Verfahren zur Quantifizierung von Tumorzellen in einer Körperflüssigkeit und dazu geeignete Testkits
AU2012332215B2 (en) Materials and method for immobilizing, isolating, and concentrating cells using carboxylated surfaces
US20200199569A1 (en) Novel affinity matrix and devices for isolation and purification of rna and dna for point of care molecular devices
ATE493508T1 (de) Verfahren, kits und vorrichtungen zur prozessierung von nukleinsäuren
GR1003966B (el) Εμβαπτιζομενος χαρτης αποξηραμενων αντιδραστηριων και μεθοδος για την ανιχνευση η και προσδιορισμο ειδικων αλληλουχιων νουκλεικων οξεων
WO2000050564A3 (en) Method for extracting dna from dried specimens
WO1996019587A3 (en) Methods of immobilizing oligonucleotides to solid support materials and methods of using support bound oligonucleotides
WO2001053817A3 (en) Methods of and apparatus for separating and detecting nucleic acid
WO2001010897A3 (en) Methods and compositions for detecting larval taenia solium
Backus et al. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
WO2000065092A3 (en) Blocking non-specific binding of granulocytes in microorganism detection
TW200630490A (en) A method for extracting nucleic acids of microorganisms from a liquid sample
WO2002029111A8 (en) Methods for introducing biomolecules and uses thereof

Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase