WO2000065092A3 - Blocking non-specific binding of granulocytes in microorganism detection - Google Patents

Blocking non-specific binding of granulocytes in microorganism detection Download PDF

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Publication number
WO2000065092A3
WO2000065092A3 PCT/US2000/010873 US0010873W WO0065092A3 WO 2000065092 A3 WO2000065092 A3 WO 2000065092A3 US 0010873 W US0010873 W US 0010873W WO 0065092 A3 WO0065092 A3 WO 0065092A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
probe
microorganism
granulocytes
specific binding
Prior art date
Application number
PCT/US2000/010873
Other languages
French (fr)
Other versions
WO2000065092A9 (en
WO2000065092A2 (en
Inventor
Curtis T Thompson
Joseph A Spidle
Donna G Albertson
Rick Segraves
Original Assignee
Science & Technology Corp
Univ New Mexico
Curtis T Thompson
Joseph A Spidle
Donna G Albertson
Rick Segraves
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Science & Technology Corp, Univ New Mexico, Curtis T Thompson, Joseph A Spidle, Donna G Albertson, Rick Segraves filed Critical Science & Technology Corp
Priority to AU46562/00A priority Critical patent/AU4656200A/en
Publication of WO2000065092A2 publication Critical patent/WO2000065092A2/en
Publication of WO2000065092A3 publication Critical patent/WO2000065092A3/en
Publication of WO2000065092A9 publication Critical patent/WO2000065092A9/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G01N1/312—Apparatus therefor for samples mounted on planar substrates

Abstract

This invention provides novel methods for detecting microorganisms is biological samples. The methods utilize in situ hybridization techniques. In one preferred embodiment, the methods involve blocking binding of nucleic acid probe(s) to granulocytes. The method typically involves contacting a biological sample with a nucleic acid probe that specifically hybridizes under stringent conditions to a nucleic acid of a microorganism and a blocking reagent that is a non-coding nucleic acid, washing the biological sample to remove probe that is not hybridized to a nucleic acid of the microorganism, and detecting the probe where the presence of remaining probe indicates the presence of the microorganism.
PCT/US2000/010873 1999-04-22 2000-04-21 Blocking non-specific binding of granulocytes in microorganism detection WO2000065092A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU46562/00A AU4656200A (en) 1999-04-22 2000-04-21 Blocking non-specific binding of granulocytes in microorganism detection

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US13086599P 1999-04-22 1999-04-22
US60/130,865 1999-04-22
US55605000A 2000-04-21 2000-04-21
US09/556,050 2000-04-21

Publications (3)

Publication Number Publication Date
WO2000065092A2 WO2000065092A2 (en) 2000-11-02
WO2000065092A3 true WO2000065092A3 (en) 2001-07-19
WO2000065092A9 WO2000065092A9 (en) 2001-09-20

Family

ID=26828899

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/010873 WO2000065092A2 (en) 1999-04-22 2000-04-21 Blocking non-specific binding of granulocytes in microorganism detection

Country Status (1)

Country Link
WO (1) WO2000065092A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4785449B2 (en) * 2005-07-21 2011-10-05 公益財団法人函館地域産業振興財団 Microbiological examination of foods by fluorescence in situ hybridization combined with culture
WO2016134217A1 (en) * 2015-02-19 2016-08-25 Surface Sensing Technologies Llc Device and method for detecting a target analyte
SG11201906699WA (en) * 2017-01-20 2019-08-27 Lifefoundry Inc Systems and methods for supporting multiple automated workflows

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0497464A1 (en) * 1991-01-31 1992-08-05 Amoco Corporation Rapid microbial diagnostics by in situ hybridization in aqueous suspension
WO1994002645A1 (en) * 1992-07-17 1994-02-03 Aprogenex, Inc. Rapid detection of biopolymers in stained specimens
WO1996036734A1 (en) * 1995-05-18 1996-11-21 Abbott Laboratories Polymeric peptide probes and uses thereof
WO1997026324A1 (en) * 1996-01-17 1997-07-24 Flinders Technologies Pty. Ltd. Solid phase enrichment of intact cells using intracellular constituents
WO1998015648A1 (en) * 1996-10-04 1998-04-16 Dako A/S Novel probes for the detection of mycobacteria
WO1999007892A1 (en) * 1997-08-08 1999-02-18 The Regents Of The University Of California Array-based detection of genetic alterations associated with disease
WO1999010533A1 (en) * 1997-08-26 1999-03-04 Microscreen B.V. Rapid detection and identification of micro-organisms

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0497464A1 (en) * 1991-01-31 1992-08-05 Amoco Corporation Rapid microbial diagnostics by in situ hybridization in aqueous suspension
WO1994002645A1 (en) * 1992-07-17 1994-02-03 Aprogenex, Inc. Rapid detection of biopolymers in stained specimens
WO1996036734A1 (en) * 1995-05-18 1996-11-21 Abbott Laboratories Polymeric peptide probes and uses thereof
WO1997026324A1 (en) * 1996-01-17 1997-07-24 Flinders Technologies Pty. Ltd. Solid phase enrichment of intact cells using intracellular constituents
WO1998015648A1 (en) * 1996-10-04 1998-04-16 Dako A/S Novel probes for the detection of mycobacteria
WO1999007892A1 (en) * 1997-08-08 1999-02-18 The Regents Of The University Of California Array-based detection of genetic alterations associated with disease
WO1999010533A1 (en) * 1997-08-26 1999-03-04 Microscreen B.V. Rapid detection and identification of micro-organisms

Also Published As

Publication number Publication date
WO2000065092A9 (en) 2001-09-20
WO2000065092A2 (en) 2000-11-02

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