JP4270610B2 - 走査型光学顕微鏡 - Google Patents

走査型光学顕微鏡 Download PDF

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Publication number
JP4270610B2
JP4270610B2 JP26956198A JP26956198A JP4270610B2 JP 4270610 B2 JP4270610 B2 JP 4270610B2 JP 26956198 A JP26956198 A JP 26956198A JP 26956198 A JP26956198 A JP 26956198A JP 4270610 B2 JP4270610 B2 JP 4270610B2
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JP
Japan
Prior art keywords
fluorescence
specimen
confocal
light
light beam
Prior art date
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Expired - Fee Related
Application number
JP26956198A
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English (en)
Japanese (ja)
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JP2000098245A (ja
JP2000098245A5 (enExample
Inventor
佳弘 島田
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olympus Corp
Original Assignee
Olympus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olympus Corp filed Critical Olympus Corp
Priority to JP26956198A priority Critical patent/JP4270610B2/ja
Priority to US09/401,203 priority patent/US6255646B1/en
Publication of JP2000098245A publication Critical patent/JP2000098245A/ja
Priority to US10/613,664 priority patent/USRE38860E1/en
Publication of JP2000098245A5 publication Critical patent/JP2000098245A5/ja
Application granted granted Critical
Publication of JP4270610B2 publication Critical patent/JP4270610B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
    • G01J3/021—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using plane or convex mirrors, parallel phase plates, or particular reflectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/12—Generating the spectrum; Monochromators
    • G01J3/14—Generating the spectrum; Monochromators using refracting elements, e.g. prisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4406—Fluorescence spectrometry
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy

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  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Length Measuring Devices By Optical Means (AREA)
JP26956198A 1998-09-24 1998-09-24 走査型光学顕微鏡 Expired - Fee Related JP4270610B2 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP26956198A JP4270610B2 (ja) 1998-09-24 1998-09-24 走査型光学顕微鏡
US09/401,203 US6255646B1 (en) 1998-09-24 1999-09-22 Scanning optical microscope
US10/613,664 USRE38860E1 (en) 1998-09-24 2003-07-02 Scanning optical microscope

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP26956198A JP4270610B2 (ja) 1998-09-24 1998-09-24 走査型光学顕微鏡

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2003322166A Division JP3529380B2 (ja) 2003-09-12 2003-09-12 走査型光学顕微鏡

Publications (3)

Publication Number Publication Date
JP2000098245A JP2000098245A (ja) 2000-04-07
JP2000098245A5 JP2000098245A5 (enExample) 2005-11-04
JP4270610B2 true JP4270610B2 (ja) 2009-06-03

Family

ID=17474098

Family Applications (1)

Application Number Title Priority Date Filing Date
JP26956198A Expired - Fee Related JP4270610B2 (ja) 1998-09-24 1998-09-24 走査型光学顕微鏡

Country Status (2)

Country Link
US (2) US6255646B1 (enExample)
JP (1) JP4270610B2 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19829981C2 (de) * 1998-07-04 2002-10-17 Zeiss Carl Jena Gmbh Verfahren und Anordnung zur konfokalen Mikroskopie
US6494576B1 (en) * 1999-09-30 2002-12-17 L'esperance, Jr. Francis A. Method and apparatus for spectrophotometry of the eye
US6459484B1 (en) * 1999-10-21 2002-10-01 Olympus Optical Co., Ltd. Scanning optical apparatus
JP4939703B2 (ja) * 2001-08-21 2012-05-30 オリンパス株式会社 走査型レーザー顕微鏡
JP4567324B2 (ja) * 2003-12-18 2010-10-20 オリンパス株式会社 レーザー走査型共焦点顕微鏡
CN100451614C (zh) * 2004-12-28 2009-01-14 财团法人工业技术研究院 光谱影像测量装置
DE102005042890B4 (de) * 2005-09-09 2007-05-31 Leica Microsystems Cms Gmbh Konfokalmikroskop und Verfahren zur Detektion mit einem Konfokalmikroskop
DE102006007170B4 (de) * 2006-02-08 2009-06-10 Sirona Dental Systems Gmbh Verfahren und Anordnung zur schnellen und robusten chromatisch konfokalen 3D-Messtechnik
GB0721343D0 (en) * 2007-10-30 2007-12-19 Perkinelmer Ltd Improvements in and relating to scanning confocal microscopy
CN101929848B (zh) * 2010-06-30 2012-04-25 北京理工大学 具有高空间分辨力的乘积共焦扫描检测方法
DE102010060747B4 (de) * 2010-11-23 2014-04-03 Leica Microsystems Cms Gmbh Konfokales Laser-Scanmikroskop zum Untersuchen einer Probe
DE102011017046A1 (de) 2011-04-14 2012-10-18 Till Photonics Gmbh Umschaltbare Mikroskopanordnung mit mehreren Detektoren
DE102014116782A1 (de) 2014-11-17 2016-05-19 Carl Zeiss Microscopy Gmbh Detektorvorrichtung für ein Mikroskop
EP3535616A4 (en) * 2016-11-03 2020-06-17 Oregon Health & Science University Multispectral super resolution microscopy
DE102018130901A1 (de) * 2018-12-04 2020-06-04 Precitec Optronik Gmbh Optische Messeinrichtung

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS53135660A (en) 1977-04-30 1978-11-27 Olympus Optical Co Ltd Fluorescent photometric microscope using laser light
GB9015793D0 (en) 1990-07-18 1990-09-05 Medical Res Council Confocal scanning optical microscope
US5127730A (en) * 1990-08-10 1992-07-07 Regents Of The University Of Minnesota Multi-color laser scanning confocal imaging system
JPH04350816A (ja) 1991-05-29 1992-12-04 Olympus Optical Co Ltd 共焦点蛍光顕微鏡
US5283433A (en) * 1992-10-05 1994-02-01 The Regents Of The University Of California Scanning confocal microscope providing a continuous display
DE4330347C2 (de) 1993-09-08 1998-04-09 Leica Lasertechnik Verwendung einer Vorrichtung zur Selektion und Detektion mindestens zweier Spektralbereiche eines Lichtstrahls
JP3568626B2 (ja) 1994-05-24 2004-09-22 オリンパス株式会社 走査型光学顕微鏡
US5885840A (en) * 1997-02-10 1999-03-23 Compucyte Corp. Multiple assays of cell specimens
EP0916981B1 (en) * 1997-11-17 2004-07-28 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Confocal spectroscopy system and method
US6134002A (en) * 1999-01-14 2000-10-17 Duke University Apparatus and method for the rapid spectral resolution of confocal images

Also Published As

Publication number Publication date
USRE38860E1 (en) 2005-11-01
JP2000098245A (ja) 2000-04-07
US6255646B1 (en) 2001-07-03

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