JP3730255B2 - イーストにおいて発現されたｎ末端に伸長した蛋白質 - Google Patents

イーストにおいて発現されたｎ末端に伸長した蛋白質 Download PDF

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Publication number
JP3730255B2
JP3730255B2 JP50150596A JP50150596A JP3730255B2 JP 3730255 B2 JP3730255 B2 JP 3730255B2 JP 50150596 A JP50150596 A JP 50150596A JP 50150596 A JP50150596 A JP 50150596A JP 3730255 B2 JP3730255 B2 JP 3730255B2
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Japan
Prior art keywords
glu
ala
peptide
yeast
sequence
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Expired - Lifetime
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JP50150596A
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English (en)
Japanese (ja)
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JPH10501695A (ja
Inventor
バド，クヌド
ブラント，ヤコブ
ベルグハム ケルドセン，トーマス
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Novo Nordisk AS
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Novo Nordisk AS
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
JP50150596A 1994-06-17 1995-06-16 イーストにおいて発現されたｎ末端に伸長した蛋白質 Expired - Lifetime JP3730255B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK71294 1994-06-17
DK0712/94 1994-06-17
PCT/DK1995/000250 WO1995035384A1 (fr) 1994-06-17 1995-06-16 Proteines comportant une extension a l'extremite n-terminale, exprimees par une levure

Publications (2)

Publication Number Publication Date
JPH10501695A JPH10501695A (ja) 1998-02-17
JP3730255B2 true JP3730255B2 (ja) 2005-12-21

Family

ID=8096676

Family Applications (1)

Application Number Title Priority Date Filing Date
JP50150596A Expired - Lifetime JP3730255B2 (ja) 1994-06-17 1995-06-16 イーストにおいて発現されたｎ末端に伸長した蛋白質

Country Status (16)

Country Link
EP (1) EP0765395A1 (fr)
JP (1) JP3730255B2 (fr)
CN (1) CN1131312C (fr)
AU (1) AU2733595A (fr)
BR (1) BR9508053A (fr)
CA (1) CA2192943A1 (fr)
CZ (1) CZ363796A3 (fr)
FI (1) FI965030A (fr)
HU (1) HUT75840A (fr)
IL (1) IL114160A (fr)
MX (1) MX9606536A (fr)
NO (1) NO965411L (fr)
PL (1) PL317761A1 (fr)
SG (1) SG47883A1 (fr)
WO (1) WO1995035384A1 (fr)
ZA (1) ZA954983B (fr)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6500645B1 (en) 1994-06-17 2002-12-31 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
ZA9610456B (en) * 1995-12-20 1997-06-20 Novo Nordisk As N-terminally extended proteins expressed in yeast
US6083910A (en) 1996-12-13 2000-07-04 Chiron Corporation Therapeutic uses of resolved intact or clipped native-sequence PDGF-BB dimers
WO1998028429A1 (fr) * 1996-12-20 1998-07-02 Novo Nordisk A/S Proteines a extension d'extremite n-terminal exprimees dans la levure
EP1049790A1 (fr) * 1998-01-23 2000-11-08 Novo Nordisk A/S Procede servant a preparer des polypeptides souhaites dans de la levure
KR20030004315A (ko) 1999-12-29 2003-01-14 노보 노르디스크 에이/에스 효모에서 개선된 발효 수율을 갖는 인슐린 선구물질 및인슐린 선구물질 유사체의 제조 방법
JP2005503141A (ja) * 2001-07-24 2005-02-03 ノボ　ノルディスク　アクティーゼルスカブ アシル化ポリペプチドを作出するための方法
US20030082671A1 (en) 2001-07-24 2003-05-01 Thomas Hoeg-Jensen Method for making acylated polypeptides
US7595172B2 (en) 2001-07-24 2009-09-29 Novo Nordisk A/S Method for making acylated polypeptides
WO2004085472A1 (fr) * 2003-03-27 2004-10-07 Novo Nordisk A/S Procede de fabrication de precurseurs de l'insuline humaine et insuline humaine
ATE517119T1 (de) 2003-12-03 2011-08-15 Novo Nordisk As Einzelketteninsulin
US9056921B2 (en) 2005-08-16 2015-06-16 Novo Nordisk A/S Method for making mature insulin polypeptides
CN101573133B (zh) * 2006-07-31 2014-08-27 诺沃-诺迪斯克有限公司 Peg化延长的胰岛素
EP2074141B1 (fr) 2006-09-22 2016-08-10 Novo Nordisk A/S Analogues de l'insuline resistants a une protease
WO2008037735A1 (fr) 2006-09-27 2008-04-03 Novo Nordisk A/S Procédé de fabrication de polypeptides d'insuline mature
WO2008062010A2 (fr) 2006-11-22 2008-05-29 Novo Nordisk A/S Procédé de fabrication de carboxypeptidases activées
JP5688969B2 (ja) 2007-07-16 2015-03-25 ノボ・ノルデイスク・エー／エス プロテアーゼに対して安定しているペグ化インスリンアナログ
ES2558930T3 (es) 2007-08-13 2016-02-09 Novo Nordisk A/S Análogos de la insulina de acción rápida
CA2695970A1 (fr) 2007-08-15 2009-02-19 Novo Nordisk A\S Analogues de l'insuline comprenant un fragment acyle et alkylene glycol
EP2178910B1 (fr) 2007-08-15 2014-10-08 Novo Nordisk A/S Insulines avec une fraction acyle comprenant des unités répétitives d'alkylène glycol contenant des acides aminés
EP2910571B1 (fr) 2008-03-18 2016-10-05 Novo Nordisk A/S Analogues de l'insuline acylés, stabilisés à la protéase
WO2009121884A1 (fr) 2008-04-01 2009-10-08 Novo Nordisk A/S Conjugués insuline-albumine
WO2010149772A1 (fr) 2009-06-26 2010-12-29 Novo Nordisk A/S Préparation contenant de l'insuline, du nicotinamide et un acide aminé
EP2504355B1 (fr) 2009-11-25 2015-07-22 Novo Nordisk A/S Procédé de fabrication de polypeptides
WO2011161125A1 (fr) 2010-06-23 2011-12-29 Novo Nordisk A/S Dérivés d'insuline contenant des liaisons disulfure supplémentaires
CN102933599A (zh) 2010-06-23 2013-02-13 诺沃—诺迪斯克有限公司 包含额外的二硫键的人胰岛素
EP2585484A1 (fr) 2010-06-23 2013-05-01 Novo Nordisk A/S Analogues de l'insuline contenant des liaisons disulfures supplémentaires
CN103249427A (zh) 2010-12-14 2013-08-14 诺沃—诺迪斯克有限公司 速效胰岛素联合长效胰岛素
RU2013130374A (ru) 2010-12-14 2015-01-20 Ново Нордиск А/С Препарат, содержащий инсулин, никотинамид и аминокислоту
US9260503B2 (en) 2011-06-15 2016-02-16 Novo Nordisk A/S Multi-substituted insulins
WO2013186138A1 (fr) 2012-06-14 2013-12-19 Novo Nordisk A/S Préparation comprenant de l'insuline, du nicotinamide et de l'arginine
WO2014088836A1 (fr) 2012-12-03 2014-06-12 Merck Sharp & Dohme Corp. Insuline et analogues d'insuline à base de peptide à partie terminale carboxy (ptc) o-glycosylée
EP3004156A1 (fr) 2013-06-07 2016-04-13 Novo Nordisk A/S Procédé de fabrication de polypeptides d'insuline mature
ES2784603T3 (es) 2015-06-02 2020-09-29 Novo Nordisk As Insulinas con extensiones recombinantes polares
JP2018531900A (ja) 2015-08-25 2018-11-01 ノヴォ ノルディスク アー／エス 新規インスリン誘導体及びその医学的使用
US20190010206A1 (en) 2015-08-25 2019-01-10 Novo Nordisk A/S Novel Insulin Derivatives and the Medical Uses Hereof
JP2018531899A (ja) 2015-08-25 2018-11-01 ノヴォ ノルディスク アー／エス 新規インスリン誘導体及びその医学的使用
US20190248852A1 (en) * 2016-05-24 2019-08-15 Novo Nordisk A/S MIC-1 Compounds and Use Thereof
AU2018317810A1 (en) 2017-08-17 2020-03-19 Novo Nordisk A/S Novel acylated insulin analogues and uses thereof
US20210230659A1 (en) * 2018-06-18 2021-07-29 Unichem Laboratories Ltd Leader Sequence for Higher Expression of Recombinant Proteins
EP4073097A1 (fr) 2019-12-11 2022-10-19 Novo Nordisk A/S Nouveaux analogues de l'insuline et leurs utilisations
WO2023144240A1 (fr) 2022-01-26 2023-08-03 Novo Nordisk Research Centre Oxford Limited Dérivés d'insuline sensibles au glucose et leurs utilisations

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2593518B1 (fr) * 1985-05-02 1989-09-08 Transgene Sa Vecteurs d'expression et de secretion de l'hirudine par les levures transformees
US5010182A (en) * 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
CA1340772C (fr) * 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression et secretion de proteines heterologues dans une levure au moyen de sequences leader tronquees du facteur alpha
DK105489D0 (da) * 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid
DK300090D0 (da) * 1990-12-19 1990-12-19 Novo Nordisk As Fremgangsmaade til fremstilling af leadersekvenser
WO1992013951A1 (fr) * 1991-02-04 1992-08-20 The Salk Institute Biotechnology/Industrial Associates, Inc. Production de serumalbumine humaine dans des cellules de levure methylotrophique

Also Published As

Publication number Publication date
IL114160A0 (en) 1995-10-31
ZA954983B (en) 1996-02-14
BR9508053A (pt) 1997-08-12
JPH10501695A (ja) 1998-02-17
PL317761A1 (en) 1997-04-28
HUT75840A (en) 1997-05-28
HU9603476D0 (en) 1997-02-28
FI965030A (fi) 1997-02-14
SG47883A1 (en) 1998-04-17
NO965411D0 (no) 1996-12-16
CZ363796A3 (en) 1997-05-14
CA2192943A1 (fr) 1995-12-28
WO1995035384A1 (fr) 1995-12-28
CN1131312C (zh) 2003-12-17
EP0765395A1 (fr) 1997-04-02
NO965411L (no) 1997-02-14
CN1152942A (zh) 1997-06-25
AU2733595A (en) 1996-01-15
FI965030A0 (fi) 1996-12-16
MX9606536A (es) 1997-03-29
IL114160A (en) 2006-12-31

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