JP2970811B2 - 原核生物細胞中にポリペプチドを調製するための発現システム - Google Patents

原核生物細胞中にポリペプチドを調製するための発現システム

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Publication number
JP2970811B2
JP2970811B2 JP63509398A JP50939888A JP2970811B2 JP 2970811 B2 JP2970811 B2 JP 2970811B2 JP 63509398 A JP63509398 A JP 63509398A JP 50939888 A JP50939888 A JP 50939888A JP 2970811 B2 JP2970811 B2 JP 2970811B2
Authority
JP
Japan
Prior art keywords
sequence
gene
fragment
pbm11
plasmid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP63509398A
Other languages
English (en)
Japanese (ja)
Other versions
JPH04502851A (ja
Inventor
エム． ローズ，ティモシー
ジェイ． フランセシニ，トーマス
ブルース，エー．グレゴリー
ウィン リウ，スオ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ONKOOGEN
Original Assignee
ONKOOGEN
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ONKOOGEN filed Critical ONKOOGEN
Publication of JPH04502851A publication Critical patent/JPH04502851A/ja
Application granted granted Critical
Publication of JP2970811B2 publication Critical patent/JP2970811B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/495—Transforming growth factor [TGF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/521—Chemokines
    • C07K14/522—Alpha-chemokines, e.g. NAP-2, ENA-78, GRO-alpha/MGSA/NAP-3, GRO-beta/MIP-2alpha, GRO-gamma/MIP-2beta, IP-10, GCP-2, MIG, PBSF, PF-4, KC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
JP63509398A 1987-10-30 1988-10-28 原核生物細胞中にポリペプチドを調製するための発現システム Expired - Lifetime JP2970811B2 (ja)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US11513987A 1987-10-30 1987-10-30
US24076888A 1988-09-02 1988-09-02
US240,768 1988-09-02
US115,139 1988-09-02

Publications (2)

Publication Number Publication Date
JPH04502851A JPH04502851A (ja) 1992-05-28
JP2970811B2 true JP2970811B2 (ja) 1999-11-02

Family

ID=26812881

Family Applications (1)

Application Number Title Priority Date Filing Date
JP63509398A Expired - Lifetime JP2970811B2 (ja) 1987-10-30 1988-10-28 原核生物細胞中にポリペプチドを調製するための発現システム

Country Status (3)

Country Link
EP (1) EP0372005A4 (de)
JP (1) JP2970811B2 (de)
WO (1) WO1989003886A1 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI891308A (fi) * 1988-03-24 1989-09-25 Oncogen Nya polypeptider med tillvaextfaktoraktivitet och dessa kodande nukleinsyrasekvenser.
US5086164A (en) * 1989-01-10 1992-02-04 Repligen Corporation Novel methods and compositions for treatment of angiogenic diseases
IE901849A1 (en) * 1989-07-19 1991-06-19 Gruenenthal Gmbh Plasmids, their preparation and their use in the manufacture¹of a plasminogen activator
EP0423641A3 (en) * 1989-10-18 1992-01-15 Nippon Mining Company Limited Expression vector, transformed microorganism, fusion protein and method for the production of platelet factor 4 or tgfalpha
JPH05219963A (ja) * 1990-07-18 1993-08-31 Applied Immune Sci Inc 温度誘導分泌発現ベクターおよびその原核細胞での使用
US6083686A (en) * 1990-10-26 2000-07-04 Johnson & Johnson Clinical Diagnostic Systems, Inc. Increased production of Thermus aquaticus DNA polymerase in E. coli
EP0502637B1 (de) * 1991-02-26 2004-04-07 AstraZeneca AB Vektor
EP1117805A2 (de) 1998-10-07 2001-07-25 STRYKER CORPORATION (a Michigan corporation) Modifizierte proteine der tgf-beta superfamilie
IL157378A0 (en) 2001-03-09 2004-02-19 Genentech Inc Process for production of polypeptides
US7153666B2 (en) 2003-07-17 2006-12-26 General Atomics Methods and compositions for determination of glycated proteins
US7022494B2 (en) 2003-09-19 2006-04-04 General Atomics Detection of potassium ions using ion-sensitive enzymes
US8187831B2 (en) 2003-09-19 2012-05-29 General Atomics Determination of ions using ion-sensitive enzymes
US7943385B2 (en) 2006-07-25 2011-05-17 General Atomics Methods for assaying percentage of glycated hemoglobin
EP2044444B1 (de) 2006-07-25 2013-03-20 General Atomics Verfahren zur bestimmung des anteils an glykiertem hämoglobin
US8673646B2 (en) 2008-05-13 2014-03-18 General Atomics Electrochemical biosensor for direct determination of percentage of glycated hemoglobin

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4565785A (en) 1978-06-08 1986-01-21 The President And Fellows Of Harvard College Recombinant DNA molecule

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL60184A (en) * 1979-05-31 1984-05-31 Schering Ag Process for the specific cleavage of protein sequences from proteins
US4582800A (en) * 1982-07-12 1986-04-15 Hoffmann-La Roche Inc. Novel vectors and method for controlling interferon expression
US4711845A (en) * 1984-08-31 1987-12-08 Cetus Corporation Portable temperature-sensitive control cassette
EP0172194B1 (de) * 1984-02-08 1994-01-19 Cetus Oncology Corporation Kontrollsysteme für rekombinant-manipulationen
DE3430683A1 (de) * 1984-08-21 1986-03-06 Hoechst Ag, 6230 Frankfurt Synthetische regulationsregion
EP0196864A3 (de) * 1985-03-25 1988-03-23 Cetus Corporation Behandlung und Sekretion von rekombinanten Proteinen mittels alkalischer Phosphatase, dazu verwendbare DNA-Sequenzen und mittels dieser Sequenzen transformierte Zellen
AU597951B2 (en) * 1986-03-27 1990-06-14 Monsanto Company Enhanced protein production in bacteria by employing a novel ribosome binding site

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4565785A (en) 1978-06-08 1986-01-21 The President And Fellows Of Harvard College Recombinant DNA molecule

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ＥＭＢＯ Ｊｏｕｒｎａｌ Ｖｏｌ．１ Ｎｏ．１０，ｐ．１２１７−１２２４
Ｉｎｔ．Ｊ．Ｐｅｐｔ．Ｐｒｏｔｅｉｎ．Ｒｅｓ．Ｖｏｌ．２５，Ｎｏ．５，ｐ５４２−５４６
Ｊ．Ｂｉｏｃｈｅｍ．９７，ｐ．１４２９−１４３６

Also Published As

Publication number Publication date
WO1989003886A1 (en) 1989-05-05
EP0372005A1 (de) 1990-06-13
EP0372005A4 (de) 1990-06-26
JPH04502851A (ja) 1992-05-28

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