JP2024510465A - スパイクタンパク質の変異を有する重症急性呼吸器症候群コロナウイルス２（ｓａｒｓ－ｃｏｖ－２）バリアントを検出するための組成物および方法 - Google Patents

スパイクタンパク質の変異を有する重症急性呼吸器症候群コロナウイルス２（ｓａｒｓ－ｃｏｖ－２）バリアントを検出するための組成物および方法 Download PDF

Info

Publication number
JP2024510465A
JP2024510465A JP2023556784A JP2023556784A JP2024510465A JP 2024510465 A JP2024510465 A JP 2024510465A JP 2023556784 A JP2023556784 A JP 2023556784A JP 2023556784 A JP2023556784 A JP 2023556784A JP 2024510465 A JP2024510465 A JP 2024510465A
Authority
JP
Japan
Prior art keywords
seq
oligonucleotide
primer
cov
sars
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2023556784A
Other languages
English (en)
Japanese (ja)
Other versions
JP2024510465A5 (https=
JPWO2022194756A5 (https=
Inventor
アール．フォンテチャ マルセル
マンギプディ カリヤニ
マノハー チトラ
デイビッド サンティーニ クリストファー
スパイアー ユージーン
スン チンタオ
エリザベス イー ミッシェル
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of JP2024510465A publication Critical patent/JP2024510465A/ja
Publication of JP2024510465A5 publication Critical patent/JP2024510465A5/ja
Publication of JPWO2022194756A5 publication Critical patent/JPWO2022194756A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
JP2023556784A 2021-03-15 2022-03-14 スパイクタンパク質の変異を有する重症急性呼吸器症候群コロナウイルス２（ｓａｒｓ－ｃｏｖ－２）バリアントを検出するための組成物および方法 Pending JP2024510465A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202163161398P 2021-03-15 2021-03-15
US63/161,398 2021-03-15
US202163168718P 2021-03-31 2021-03-31
US63/168,718 2021-03-31
PCT/EP2022/056496 WO2022194756A2 (en) 2021-03-15 2022-03-14 Compositions and methods for detecting severe acute respiratory syndrome coronavirus 2 (sars-cov-2) variants having spike protein mutations

Publications (3)

Publication Number Publication Date
JP2024510465A true JP2024510465A (ja) 2024-03-07
JP2024510465A5 JP2024510465A5 (https=) 2025-01-23
JPWO2022194756A5 JPWO2022194756A5 (https=) 2025-01-23

Family

ID=81326309

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023556784A Pending JP2024510465A (ja) 2021-03-15 2022-03-14 スパイクタンパク質の変異を有する重症急性呼吸器症候群コロナウイルス２（ｓａｒｓ－ｃｏｖ－２）バリアントを検出するための組成物および方法

Country Status (4)

Country Link
US (1) US20220290221A1 (https=)
EP (1) EP4308726A2 (https=)
JP (1) JP2024510465A (https=)
WO (1) WO2022194756A2 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20220042117A1 (en) * 2020-08-06 2022-02-10 Roche Molecular Systems, Inc. COMPOSITIONS AND METHODS FOR THE SIMULTANEOUS DETECTION OF INFLUENZA A, INFLUENZA B, AND SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS 2 (SARS-CoV-2)
US20240409984A1 (en) * 2023-06-09 2024-12-12 Revvity Health Sciences, Inc. Methods for detecting mutations in target nucleic acids using dual probes

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017518028A (ja) * 2014-03-28 2017-07-06 シージーン アイエヌシー 相異なる検出温度を利用したターゲット核酸配列の検出
JP2018514202A (ja) * 2015-04-17 2018-06-07 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 遺伝子融合体を検出するための多重ｐｃｒ

Family Cites Families (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0135587B2 (en) 1983-02-22 2002-12-18 Syngene, Inc. Defined sequence single strand oligonucleotides incorporating reporter groups, process for the chemical synthesis thereof, and nucleosides useful in such synthesis
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4996143A (en) 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US5683896A (en) 1989-06-01 1997-11-04 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5035996A (en) 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
EP1067134B1 (en) 1991-11-07 2004-07-28 Nanotronics, Inc. Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to-donor energy transfer system
EP1033411B1 (en) 1996-06-04 2006-02-22 University of Utah Research Foundation Fluorescent donor-acceptor pair
JP4281877B2 (ja) 1996-06-04 2009-06-17 ユニバーシティ オブ ユタ リサーチ ファウンデーション 生物学的プロセスを実行し且つモニタリングするためのシステムと方法
JP3756313B2 (ja) 1997-03-07 2006-03-15 武 今西 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体
ATE280177T1 (de) * 1997-03-20 2004-11-15 Hoffmann La Roche Modifizierte primer
US6794499B2 (en) 1997-09-12 2004-09-21 Exiqon A/S Oligonucleotide analogues
WO2005092038A2 (en) * 2004-03-22 2005-10-06 The Johns Hopkins University Methods for the detection of nucleic acid differences
WO2007039301A2 (en) 2005-10-05 2007-04-12 Roche Diagnostics Gmbh Non-fluorescent energy transfer
EP2914753A4 (en) * 2012-10-31 2016-06-08 Becton Dickinson Co SELECTIVE AMPLIFICATION AND REAL-TIME PCR DETECTION OF RARE MUTATIONS
US20150176060A1 (en) * 2013-12-20 2015-06-25 Roche Molecular Systems, Inc. Method For Coding Of Multiple PCR Reactions For Assay Recognition
EP3286334A4 (en) * 2015-04-20 2018-09-12 Neogenomics Laboratories, Inc. Method to increase sensitivity of next generation sequencing
EP3286323B1 (en) * 2015-04-24 2023-01-18 Becton, Dickinson and Company Multiplex detection of bacterial vaginosis
RU2674338C1 (ru) * 2017-10-09 2018-12-07 Федеральное Государственное Бюджетное Учреждение Науки Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук (Имб Ран) Способ анализа соматических мутаций в генах BRAF, NRAS и KIT с использованием LNA-блокирующей мультиплексной ПЦР и последующей гибридизацией с олигонуклеотидным биологическим микрочипом (биочипом)
WO2021152181A1 (en) * 2020-01-30 2021-08-05 Biomedrex Ab Virus therapy
CN110982945B (zh) * 2020-03-04 2020-06-09 珠海丽珠试剂股份有限公司 一种检测2019新型冠状病毒的核酸组合物、试剂盒以及方法
WO2021188881A2 (en) * 2020-03-20 2021-09-23 Applied Dna Sciences, Inc. Compositions and methods for detecting and treating sars-cov-2
WO2021202158A1 (en) * 2020-04-01 2021-10-07 President And Fellows Of Harvard College Nested rpa and competitive reference standard
CN112111597B (zh) * 2020-04-28 2023-07-21 安徽同科生物科技有限公司 一种免核酸提取快速检测新型冠状病毒2019-nCoV核酸的荧光PCR试剂盒
CN111254228B (zh) * 2020-05-06 2020-09-11 上海思路迪医学检验所有限公司 一种检测新型冠状病毒和流感病毒的试剂盒
CN111560478B (zh) * 2020-05-27 2020-11-13 广州凯普医药科技有限公司 一种一步法反转录PCR结合Sanger测序检测新型冠状病毒的试剂盒
CN111534643B (zh) * 2020-07-10 2020-10-09 上海科技大学 一种检测呼吸道病原的核酸的试剂盒、检测方法及应用
WO2022020393A1 (en) * 2020-07-20 2022-01-27 Mammoth Biosciences, Inc. High-throughput single-chamber programmable nuclease assay
WO2022044011A1 (en) * 2020-08-25 2022-03-03 B. G. Negev Technologies And Applications Ltd., At Ben-Gurion University Oligonucleotides and methods of using same
CN113637798A (zh) * 2021-08-09 2021-11-12 南昌大学 检测新冠病毒Alpha毒株S基因Δ69/70HV缺失突变位点的引物、探针及其应用

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017518028A (ja) * 2014-03-28 2017-07-06 シージーン アイエヌシー 相異なる検出温度を利用したターゲット核酸配列の検出
JP2018514202A (ja) * 2015-04-17 2018-06-07 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 遺伝子融合体を検出するための多重ｐｃｒ

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DOMINIK NORZ ET AL.: "Evaluation of a fully automated high-throughput SARS-CoV-2 multiplex qPCR assay with buil", MEDRXIV, JPN7025005631, 18 February 2021 (2021-02-18), ISSN: 0005765934 *
NANCY J SCHOENBRUNNER ET AL.: "Covalent modification of primers improves PCR amplification specificity and yield", BIOLOGY METHODS AND PROTOCOLS, vol. 2017, JPN7025005630, 21 November 2017 (2017-11-21), pages 011, ISSN: 0005765935 *

Also Published As

Publication number Publication date
EP4308726A2 (en) 2024-01-24
WO2022194756A2 (en) 2022-09-22
US20220290221A1 (en) 2022-09-15
WO2022194756A3 (en) 2022-12-01

Similar Documents

Publication Publication Date Title
JP7796660B2 (ja) 重症急性呼吸器症候群コロナウイルス２（ｓａｒｓ－ｃｏｖ－２）、インフルエンザａ及びインフルエンザｂを検出するための組成物及び方法
JP7141488B2 (ja) マイコプラズマ・ジェニタリウムを検出するための組成物と方法
US11767567B2 (en) Compositions and methods for detection of Babesia
JP7478734B2 (ja) カンジダ・アウリス（ｃａｎｄｉｄａ ａｕｒｉｓ）の検出のための組成物および方法
JP6962927B2 (ja) ジカウイルス検出用組成物および方法
JP2024510465A (ja) スパイクタンパク質の変異を有する重症急性呼吸器症候群コロナウイルス２（ｓａｒｓ－ｃｏｖ－２）バリアントを検出するための組成物および方法
JP2015529090A (ja) クロストリジウム−ディフィシルの検出のための組成物及び方法
JP7649285B2 (ja) エプスタイン・バーウイルス（ｅｂｖ）を検出するための組成物および方法
US20240240273A1 (en) Compositions and Methods for Detecting Hepatitis Delta Virus by a Dual-Target Assay
WO2024042042A1 (en) Compositions and methods for detecting monkeypox virus
JP2023552546A (ja) マラリアを検出するための組成物および方法
JP6999645B2 (ja) 核酸の増幅及び検出／定量の効率を改良するためのヘルパーオリゴヌクレオチド
CN117441029A (zh) 用于检测具有刺突蛋白突变的严重急性呼吸综合征冠状病毒2(sars-cov-2)变体的组合物和方法
US20250003013A1 (en) Compositions and Methods for Detection of Malaria
CA2994606C (en) Compositions and methods for detection of mycobacterium tuberculosis
JP2024505686A (ja) ヒトパラインフルエンザウイルス１－４（ｈｐｉｖ１－４）の検出のための組成物及び方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250114

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20250114

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20251217

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20260106

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20260324