JP2022539338A - 改良された相同性依存性修復ゲノム編集 - Google Patents

改良された相同性依存性修復ゲノム編集 Download PDF

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JP2022539338A
JP2022539338A JP2021576666A JP2021576666A JP2022539338A JP 2022539338 A JP2022539338 A JP 2022539338A JP 2021576666 A JP2021576666 A JP 2021576666A JP 2021576666 A JP2021576666 A JP 2021576666A JP 2022539338 A JP2022539338 A JP 2022539338A
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protein
sequence
exonuclease
stranded dna
cell
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JP2022539338A5 (enExample
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トマーシュ チェルマク，
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イナリ アグリカルチャー テクノロジー， インコーポレイテッド
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    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2021576666A 2019-06-25 2020-06-24 改良された相同性依存性修復ゲノム編集 Pending JP2022539338A (ja)

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US201962866317P 2019-06-25 2019-06-25
US62/866,317 2019-06-25
PCT/US2020/039410 WO2020264016A1 (en) 2019-06-25 2020-06-24 Improved homology dependent repair genome editing

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JP2022539338A true JP2022539338A (ja) 2022-09-08
JP2022539338A5 JP2022539338A5 (enExample) 2023-07-04

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US (2) US11041172B2 (enExample)
EP (1) EP3990633A1 (enExample)
JP (1) JP2022539338A (enExample)
KR (1) KR102963335B1 (enExample)
CN (2) CN120665955A (enExample)
AR (1) AR123323A1 (enExample)
AU (1) AU2020301236A1 (enExample)
BR (1) BR112021026220A2 (enExample)
CA (1) CA3138663A1 (enExample)
IL (1) IL289106A (enExample)
MX (1) MX2021014861A (enExample)
SG (1) SG11202112092TA (enExample)
WO (1) WO2020264016A1 (enExample)

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CN115976086B (zh) * 2023-01-19 2023-08-08 河南农业大学 一种细菌CRISPR-Cas9基因编辑的方法和应用
WO2024168265A1 (en) * 2023-02-10 2024-08-15 Possible Medicines Llc Aav delivery of rna guided recombination system
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CN116376961A (zh) * 2023-04-24 2023-07-04 深圳大学 同源重组酶在莱茵衣藻基因组编辑中的应用
CN116873928B (zh) * 2023-06-28 2025-08-05 浙江工业大学 一种碳化钨纳米棒-碳纳米片复合材料、铁碳微电极材料的制备方法和应用
CN116555346B (zh) * 2023-07-05 2023-09-01 中山大学 一种采用碳纳米管基因载体递送系统促进草鱼生长的方法
KR102972178B1 (ko) * 2023-11-03 2026-06-02 한경국립대학교 산학협력단 벼에서 CRISPR/Cas9-제미니바이러스 레플리콘 기반의 상동지정 복구 효율 증진 방법
CN117487959B (zh) * 2024-01-02 2024-03-22 东北农业大学 一种与印度南瓜侧枝数量相关基因连锁的分子标记及应用
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