JP2021507694A5 - - Google Patents
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- Publication number
- JP2021507694A5 JP2021507694A5 JP2020532631A JP2020532631A JP2021507694A5 JP 2021507694 A5 JP2021507694 A5 JP 2021507694A5 JP 2020532631 A JP2020532631 A JP 2020532631A JP 2020532631 A JP2020532631 A JP 2020532631A JP 2021507694 A5 JP2021507694 A5 JP 2021507694A5
- Authority
- JP
- Japan
- Prior art keywords
- dna polymerase
- seq
- sequence
- amino acid
- aspartic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 38
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 38
- CKLJMWTZIZZHCS-REOHCLBHSA-N aspartic acid group Chemical group N[C@@H](CC(=O)O)C(=O)O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims 19
- 108020004707 nucleic acids Proteins 0.000 claims 15
- 102000039446 nucleic acids Human genes 0.000 claims 15
- 150000007523 nucleic acids Chemical class 0.000 claims 15
- 238000000034 method Methods 0.000 claims 9
- 125000000539 amino acid group Chemical group 0.000 claims 8
- 150000001413 amino acids Chemical group 0.000 claims 8
- 239000002773 nucleotide Substances 0.000 claims 8
- 125000003729 nucleotide group Chemical group 0.000 claims 8
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 6
- 108091034117 Oligonucleotide Proteins 0.000 claims 4
- 235000003704 aspartic acid Nutrition 0.000 claims 4
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims 4
- 238000006243 chemical reaction Methods 0.000 claims 4
- 239000013615 primer Substances 0.000 claims 4
- 239000011541 reaction mixture Substances 0.000 claims 4
- 125000003295 alanine group Chemical group N[C@@H](C)C(=O)* 0.000 claims 3
- 239000013604 expression vector Substances 0.000 claims 3
- 239000001963 growth medium Substances 0.000 claims 3
- 239000003155 DNA primer Substances 0.000 claims 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
- 235000004279 alanine Nutrition 0.000 claims 2
- 230000003321 amplification Effects 0.000 claims 2
- 238000000137 annealing Methods 0.000 claims 2
- 230000000694 effects Effects 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims 2
- 238000006116 polymerization reaction Methods 0.000 claims 2
- 241000700605 Viruses Species 0.000 claims 1
- 239000007853 buffer solution Substances 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 108091033319 polynucleotide Proteins 0.000 claims 1
- 102000040430 polynucleotide Human genes 0.000 claims 1
- 239000002157 polynucleotide Substances 0.000 claims 1
- 235000018102 proteins Nutrition 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 238000003259 recombinant expression Methods 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
- 238000012163 sequencing technique Methods 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
- 239000006228 supernatant Substances 0.000 claims 1
- 238000013518 transcription Methods 0.000 claims 1
- 230000035897 transcription Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1721053.5 | 2017-12-15 | ||
| GBGB1721053.5A GB201721053D0 (en) | 2017-12-15 | 2017-12-15 | DNA polymerases |
| PCT/EP2018/085342 WO2019115834A1 (en) | 2017-12-15 | 2018-12-17 | Dna polymerases |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2021507694A JP2021507694A (ja) | 2021-02-25 |
| JP2021507694A5 true JP2021507694A5 (enExample) | 2022-01-06 |
| JP7361694B2 JP7361694B2 (ja) | 2023-10-16 |
Family
ID=61008786
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020532631A Active JP7361694B2 (ja) | 2017-12-15 | 2018-12-17 | Ｄｎａポリメラーゼ |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US11390857B2 (enExample) |
| EP (1) | EP3724349A1 (enExample) |
| JP (1) | JP7361694B2 (enExample) |
| KR (1) | KR102594174B1 (enExample) |
| CN (1) | CN111433373B (enExample) |
| AU (1) | AU2018382619B2 (enExample) |
| BR (1) | BR112020011605A2 (enExample) |
| CA (1) | CA3085558C (enExample) |
| GB (1) | GB201721053D0 (enExample) |
| SG (1) | SG11202004757RA (enExample) |
| WO (1) | WO2019115834A1 (enExample) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20240150735A1 (en) * | 2021-06-21 | 2024-05-09 | Amazon Technologies, Inc. | Polymerases for isothermal nucleic acid amplification |
| WO2024138419A1 (zh) * | 2022-12-28 | 2024-07-04 | 深圳华大生命科学研究院 | 具有dna聚合酶活性的多肽及其应用 |
| CN116103263A (zh) * | 2023-02-13 | 2023-05-12 | 安序源生物科技(深圳)有限公司 | 突变型pwt聚合酶及其制备方法与应用 |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6818431B1 (en) | 1995-10-18 | 2004-11-16 | Shanghai Mendel Dna Center Co. Ltd. | DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides |
| US6165765A (en) | 1995-10-18 | 2000-12-26 | Shanghai Institute Of Biochemistry, Chinese Academy Of Sciences | DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides |
| KR100269979B1 (ko) | 1998-04-15 | 2000-11-01 | 박한오 | 써무스 필리포미스로부터 분리된 내열성 dna 중합효소의 유전자 및 그로부터 유추되는 아미노산 서열 |
| US6479267B1 (en) * | 1998-06-17 | 2002-11-12 | Amersham Pharmacia Biotech, Inc. | FY7 polymerase |
| ATE299940T1 (de) * | 1999-08-21 | 2005-08-15 | Amersham Biosciences Corp | Taq dna-polymerase mit einer aminosäuresubstitution in position e681 und entsprechende homologe mit einer verbesserten salztoleranz, nützlich für die sequenzierung schwieriger templatesequenzen mittels geladenen oder ungeladenen terminatoren |
| WO2006030455A1 (en) | 2004-09-17 | 2006-03-23 | Prokaria Ehf. | Dna polymerases having strand displacement activity |
| US20080311626A1 (en) | 2004-09-17 | 2008-12-18 | Arkea Hf | Dna Polymerases Having Strand Displacement Activity |
| ATE537251T1 (de) * | 2005-10-06 | 2011-12-15 | Lucigen Corp | Temperaturstabile virale polymerasen und verwendungsverfahren |
| WO2007046556A1 (ja) * | 2005-10-20 | 2007-04-26 | Riken | 新規dnaポリメラーゼ |
| AU2011220536B2 (en) | 2010-02-26 | 2012-05-24 | Life Technologies Corporation | Modified proteins and methods of making and using same |
| LT2742151T (lt) * | 2011-08-10 | 2018-02-12 | Life Technologies Corporation | Polimerazės kompozicija |
| US8993298B1 (en) | 2012-08-31 | 2015-03-31 | New England Biolabs, Inc. | DNA polymerases |
| EP4219739A3 (en) * | 2013-09-30 | 2023-08-23 | Life Technologies Corporation | Polymerase compositions, methods of making and using same |
| GB201414745D0 (en) * | 2014-08-19 | 2014-10-01 | Articzymes As | Exonucleases |
| WO2016077324A1 (en) | 2014-11-11 | 2016-05-19 | Life Technologies Corporation | Thiolated nucleotide analogues for nucleic acid synthesis |
| US11091745B2 (en) | 2015-05-12 | 2021-08-17 | Dna Polymerase Technology, Inc. | Mutant polymerases and uses thereof |
| ES2925905T3 (es) | 2015-10-01 | 2022-10-20 | Life Technologies Corp | Composiciones y kits de polimerasa, y métodos de uso y fabricación de los mismos |
| JP6963238B2 (ja) * | 2015-11-27 | 2021-11-05 | 国立大学法人九州大学 | Ｄｎａポリメラーゼ変異体 |
| JP6959931B2 (ja) | 2016-02-29 | 2021-11-05 | ジェニア・テクノロジーズ・インコーポレイテッド | エキソヌクレアーゼ欠損ポリメラーゼ |
| GB201604876D0 (en) * | 2016-03-22 | 2016-05-04 | Uni I Tromsø Norges Arktiske Uni | Marine DNA polymerases |
-
2017
- 2017-12-15 GB GBGB1721053.5A patent/GB201721053D0/en not_active Ceased
-
2018
- 2018-12-17 JP JP2020532631A patent/JP7361694B2/ja active Active
- 2018-12-17 CN CN201880079055.2A patent/CN111433373B/zh active Active
- 2018-12-17 SG SG11202004757RA patent/SG11202004757RA/en unknown
- 2018-12-17 EP EP18825977.4A patent/EP3724349A1/en active Pending
- 2018-12-17 CA CA3085558A patent/CA3085558C/en active Active
- 2018-12-17 AU AU2018382619A patent/AU2018382619B2/en active Active
- 2018-12-17 KR KR1020207020080A patent/KR102594174B1/ko active Active
- 2018-12-17 US US16/772,690 patent/US11390857B2/en active Active
- 2018-12-17 BR BR112020011605-0A patent/BR112020011605A2/pt unknown
- 2018-12-17 WO PCT/EP2018/085342 patent/WO2019115834A1/en not_active Ceased
-
2022
- 2022-06-10 US US17/838,078 patent/US20230034466A1/en not_active Abandoned
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