AU2018382619B2 - DNA polymerases - Google Patents

DNA polymerases Download PDF

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Publication number
AU2018382619B2
AU2018382619B2 AU2018382619A AU2018382619A AU2018382619B2 AU 2018382619 B2 AU2018382619 B2 AU 2018382619B2 AU 2018382619 A AU2018382619 A AU 2018382619A AU 2018382619 A AU2018382619 A AU 2018382619A AU 2018382619 B2 AU2018382619 B2 AU 2018382619B2
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AU
Australia
Prior art keywords
dna polymerase
seq
leu
glu
ala
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AU2018382619A
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AU2018382619A1 (en
Inventor
Atle Noralf Larsen
Yvonne PIOTROWSKI
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Universitetet I Tromso UIT
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Universitetet I Tromso UIT
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
    • Y02A90/10—Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2018382619A 2017-12-15 2018-12-17 DNA polymerases Active AU2018382619B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1721053.5 2017-12-15
GBGB1721053.5A GB201721053D0 (en) 2017-12-15 2017-12-15 DNA polymerases
PCT/EP2018/085342 WO2019115834A1 (en) 2017-12-15 2018-12-17 Dna polymerases

Publications (2)

Publication Number Publication Date
AU2018382619A1 AU2018382619A1 (en) 2020-06-11
AU2018382619B2 true AU2018382619B2 (en) 2022-03-31

Family

ID=61008786

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2018382619A Active AU2018382619B2 (en) 2017-12-15 2018-12-17 DNA polymerases

Country Status (11)

Country Link
US (2) US11390857B2 (enExample)
EP (1) EP3724349A1 (enExample)
JP (1) JP7361694B2 (enExample)
KR (1) KR102594174B1 (enExample)
CN (1) CN111433373B (enExample)
AU (1) AU2018382619B2 (enExample)
BR (1) BR112020011605A2 (enExample)
CA (1) CA3085558C (enExample)
GB (1) GB201721053D0 (enExample)
SG (1) SG11202004757RA (enExample)
WO (1) WO2019115834A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20240150735A1 (en) * 2021-06-21 2024-05-09 Amazon Technologies, Inc. Polymerases for isothermal nucleic acid amplification
WO2024138419A1 (zh) * 2022-12-28 2024-07-04 深圳华大生命科学研究院 具有dna聚合酶活性的多肽及其应用
CN116103263A (zh) * 2023-02-13 2023-05-12 安序源生物科技(深圳)有限公司 突变型pwt聚合酶及其制备方法与应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015048763A1 (en) * 2013-09-30 2015-04-02 Life Technologies Corporation Polymerase compositions, methods of making and using same

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6818431B1 (en) 1995-10-18 2004-11-16 Shanghai Mendel Dna Center Co. Ltd. DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
US6165765A (en) 1995-10-18 2000-12-26 Shanghai Institute Of Biochemistry, Chinese Academy Of Sciences DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
KR100269979B1 (ko) 1998-04-15 2000-11-01 박한오 써무스 필리포미스로부터 분리된 내열성 dna 중합효소의 유전자 및 그로부터 유추되는 아미노산 서열
US6479267B1 (en) * 1998-06-17 2002-11-12 Amersham Pharmacia Biotech, Inc. FY7 polymerase
ATE299940T1 (de) * 1999-08-21 2005-08-15 Amersham Biosciences Corp Taq dna-polymerase mit einer aminosäuresubstitution in position e681 und entsprechende homologe mit einer verbesserten salztoleranz, nützlich für die sequenzierung schwieriger templatesequenzen mittels geladenen oder ungeladenen terminatoren
WO2006030455A1 (en) 2004-09-17 2006-03-23 Prokaria Ehf. Dna polymerases having strand displacement activity
US20080311626A1 (en) 2004-09-17 2008-12-18 Arkea Hf Dna Polymerases Having Strand Displacement Activity
ATE537251T1 (de) * 2005-10-06 2011-12-15 Lucigen Corp Temperaturstabile virale polymerasen und verwendungsverfahren
WO2007046556A1 (ja) * 2005-10-20 2007-04-26 Riken 新規dnaポリメラーゼ
AU2011220536B2 (en) 2010-02-26 2012-05-24 Life Technologies Corporation Modified proteins and methods of making and using same
LT2742151T (lt) * 2011-08-10 2018-02-12 Life Technologies Corporation Polimerazės kompozicija
US8993298B1 (en) 2012-08-31 2015-03-31 New England Biolabs, Inc. DNA polymerases
GB201414745D0 (en) * 2014-08-19 2014-10-01 Articzymes As Exonucleases
WO2016077324A1 (en) 2014-11-11 2016-05-19 Life Technologies Corporation Thiolated nucleotide analogues for nucleic acid synthesis
US11091745B2 (en) 2015-05-12 2021-08-17 Dna Polymerase Technology, Inc. Mutant polymerases and uses thereof
ES2925905T3 (es) 2015-10-01 2022-10-20 Life Technologies Corp Composiciones y kits de polimerasa, y métodos de uso y fabricación de los mismos
JP6963238B2 (ja) * 2015-11-27 2021-11-05 国立大学法人九州大学 Ｄｎａポリメラーゼ変異体
JP6959931B2 (ja) 2016-02-29 2021-11-05 ジェニア・テクノロジーズ・インコーポレイテッド エキソヌクレアーゼ欠損ポリメラーゼ
GB201604876D0 (en) * 2016-03-22 2016-05-04 Uni I Tromsø Norges Arktiske Uni Marine DNA polymerases

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015048763A1 (en) * 2013-09-30 2015-04-02 Life Technologies Corporation Polymerase compositions, methods of making and using same

Also Published As

Publication number Publication date
KR20200108283A (ko) 2020-09-17
CN111433373A (zh) 2020-07-17
CA3085558C (en) 2023-01-10
US20230034466A1 (en) 2023-02-02
JP7361694B2 (ja) 2023-10-16
GB201721053D0 (en) 2018-01-31
BR112020011605A2 (pt) 2020-12-08
CA3085558A1 (en) 2019-06-20
EP3724349A1 (en) 2020-10-21
SG11202004757RA (en) 2020-07-29
WO2019115834A1 (en) 2019-06-20
JP2021507694A (ja) 2021-02-25
KR102594174B1 (ko) 2023-10-25
US11390857B2 (en) 2022-07-19
AU2018382619A1 (en) 2020-06-11
CN111433373B (zh) 2024-01-12
US20200325459A1 (en) 2020-10-15

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