ATE537251T1 - Temperaturstabile virale polymerasen und verwendungsverfahren - Google Patents
Temperaturstabile virale polymerasen und verwendungsverfahrenInfo
- Publication number
- ATE537251T1 ATE537251T1 AT06836235T AT06836235T ATE537251T1 AT E537251 T1 ATE537251 T1 AT E537251T1 AT 06836235 T AT06836235 T AT 06836235T AT 06836235 T AT06836235 T AT 06836235T AT E537251 T1 ATE537251 T1 AT E537251T1
- Authority
- AT
- Austria
- Prior art keywords
- activity
- polymerases
- methods
- temperature stable
- viral polymerases
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 230000003612 virological effect Effects 0.000 title abstract 2
- 230000000694 effects Effects 0.000 abstract 7
- 108060002716 Exonuclease Proteins 0.000 abstract 1
- 102100034343 Integrase Human genes 0.000 abstract 1
- 101710163270 Nuclease Proteins 0.000 abstract 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 abstract 1
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 238000006073 displacement reaction Methods 0.000 abstract 1
- 230000001747 exhibiting effect Effects 0.000 abstract 1
- 102000013165 exonuclease Human genes 0.000 abstract 1
- 238000010348 incorporation Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 230000001915 proofreading effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US72420705P | 2005-10-06 | 2005-10-06 | |
| US80569506P | 2006-06-23 | 2006-06-23 | |
| PCT/US2006/039406 WO2007044671A2 (en) | 2005-10-06 | 2006-10-06 | Thermostable viral polymerases and methods of use |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE537251T1 true ATE537251T1 (de) | 2011-12-15 |
Family
ID=37820562
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06836235T ATE537251T1 (de) | 2005-10-06 | 2006-10-06 | Temperaturstabile virale polymerasen und verwendungsverfahren |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US8093030B2 (de) |
| EP (2) | EP2377929A1 (de) |
| JP (1) | JP2009511019A (de) |
| AT (1) | ATE537251T1 (de) |
| CA (1) | CA2624324A1 (de) |
| WO (1) | WO2007044671A2 (de) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120083018A1 (en) * | 2005-10-06 | 2012-04-05 | Schoenfeld Thomas W | Thermostable dna polymerases and methods of use |
| US20100291638A1 (en) * | 2005-10-06 | 2010-11-18 | Thomas William Schoenfeld | Thermostable dna polymerases and methods of use |
| FR2929011B1 (fr) * | 2008-03-20 | 2013-01-04 | Commissariat Energie Atomique | Procede et dispositif de mesure quantitative a haute cadence de cibles biomoleculaires presentes sur ou dans un support d'analyse biologique. |
| GB0901593D0 (en) | 2009-01-30 | 2009-03-11 | Touchlight Genetics Ltd | Production of closed linear DNA |
| JP2013516192A (ja) * | 2010-01-08 | 2013-05-13 | キアジェン ゲイサーズバーグ インコーポレイテッド | 等温核酸増幅のための材料および方法 |
| US9523120B2 (en) | 2011-06-14 | 2016-12-20 | University of Pittsburgh—of the Commonwealth System of Higher Education | Method of amplifying a nucleic acid |
| SG11201610134PA (en) | 2014-06-06 | 2017-01-27 | Univ Cornell | Method for identification and enumeration of nucleic acid sequence, expression, copy, or dna methylation changes, using combined nuclease, ligase, polymerase, and sequencing reactions |
| WO2018009729A2 (en) * | 2016-07-06 | 2018-01-11 | Dna2.0, Inc. Dba Atum | Modification of dna polymerases for in vitro applications |
| ES2870639T3 (es) * | 2016-10-24 | 2021-10-27 | Geneinfosec Inc | Ocultación de información presente en los ácidos nucleicos |
| US11236384B2 (en) | 2017-01-19 | 2022-02-01 | Asuragen, Inc. | Methods of RNA amplification |
| GB201721053D0 (en) * | 2017-12-15 | 2018-01-31 | Univ I Tromsø - Norges Arktiske Univ | DNA polymerases |
| EP3784685A1 (de) * | 2018-05-02 | 2021-03-03 | Qiagen Beverly, LLC | Temperaturstabile virale reverse transkriptase |
| US20250059575A1 (en) | 2020-05-26 | 2025-02-20 | Qiagen Beverly Llc | Polymerase enzyme |
Family Cites Families (57)
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| US4711955A (en) * | 1981-04-17 | 1987-12-08 | Yale University | Modified nucleotides and methods of preparing and using same |
| US4994373A (en) * | 1983-01-27 | 1991-02-19 | Enzo Biochem, Inc. | Method and structures employing chemically-labelled polynucleotide probes |
| CA1228811A (en) | 1983-05-05 | 1987-11-03 | Robert G. Pergolizzi | Assay method utilizing polynucleotide sequences |
| US4748111A (en) * | 1984-03-12 | 1988-05-31 | Molecular Diagnostics, Inc. | Nucleic acid-protein conjugate used in immunoassay |
| US4883750A (en) * | 1984-12-13 | 1989-11-28 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
| US5242794A (en) | 1984-12-13 | 1993-09-07 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
| US4965188A (en) * | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4795699A (en) * | 1987-01-14 | 1989-01-03 | President And Fellows Of Harvard College | T7 DNA polymerase |
| CA1340807C (en) * | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
| US5130238A (en) * | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| GB8818020D0 (en) | 1988-07-28 | 1988-09-01 | Ici Plc | Method for amplification of nucleotide sequences |
| US5629158A (en) * | 1989-03-22 | 1997-05-13 | Cemu Bitecknik Ab | Solid phase diagnosis of medical conditions |
| US5198543A (en) * | 1989-03-24 | 1993-03-30 | Consejo Superior Investigaciones Cientificas | PHI29 DNA polymerase |
| US5001050A (en) * | 1989-03-24 | 1991-03-19 | Consejo Superior Investigaciones Cientificas | PHφ29 DNA polymerase |
| US5043272A (en) * | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
| US5106727A (en) | 1989-04-27 | 1992-04-21 | Life Technologies, Inc. | Amplification of nucleic acid sequences using oligonucleotides of random sequences as primers |
| EP0504321B1 (de) | 1989-12-04 | 1997-05-07 | Microprobe Corporation | Verstärkter einfang von ziel-nukleinsäuren mittels verwendung von oligonukleotiden, die kovalent an polymere gebunden sind |
| DE69032543T2 (de) * | 1989-12-22 | 1999-04-15 | F. Hoffmann-La Roche Ag, Basel | Rekombinante expressionsvektoren und reinigungsverfahren für dns-polymerase aus thermus thermophilus |
| US5427930A (en) * | 1990-01-26 | 1995-06-27 | Abbott Laboratories | Amplification of target nucleic acids using gap filling ligase chain reaction |
| ATE137269T1 (de) | 1990-01-26 | 1996-05-15 | Abbott Lab | Verbessertes verfahren zur amplifikation von nuklein säurezielsequenz, einsetzbar für die polymerase und ligasekettenreaktion |
| CA2087256A1 (en) | 1990-07-25 | 1992-01-26 | Jerry L. Ruth | Circular extension for generating multiple nucleic acid complements |
| US5489523A (en) * | 1990-12-03 | 1996-02-06 | Stratagene | Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I |
| US5455166A (en) * | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
| JP3080178B2 (ja) | 1991-02-18 | 2000-08-21 | 東洋紡績株式会社 | 核酸配列の増幅方法およびそのための試薬キット |
| JP3085409B2 (ja) | 1991-03-29 | 2000-09-11 | 東洋紡績株式会社 | 標的核酸配列の検出方法およびそのための試薬キット |
| US5273638A (en) * | 1991-09-30 | 1993-12-28 | Beckman Instruments, Inc. | Nucleotide sequence determination employing matched dideoxynucleotide terminator concentrations |
| US5221065A (en) * | 1992-04-16 | 1993-06-22 | Caterpillar Inc. | Mounting bracket for restraint of elongate articles |
| US5547843A (en) * | 1992-07-17 | 1996-08-20 | Associated Universities, Inc. | Method for promoting specific alignment of short oligonucleotides on nucleic acids |
| WO1994003624A1 (en) * | 1992-08-04 | 1994-02-17 | Auerbach Jeffrey I | Methods for the isothermal amplification of nucleic acid molecules |
| US5733733A (en) * | 1992-08-04 | 1998-03-31 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
| US5614389A (en) * | 1992-08-04 | 1997-03-25 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
| US5714320A (en) * | 1993-04-15 | 1998-02-03 | University Of Rochester | Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides |
| US5462854A (en) | 1993-04-19 | 1995-10-31 | Beckman Instruments, Inc. | Inverse linkage oligonucleotides for chemical and enzymatic processes |
| KR100189229B1 (ko) | 1993-07-23 | 1999-06-01 | 다니엘 엘. 캐시앙, 헨리 엘. 노르호프 | 핵산 증폭을 강화하는 방법 |
| EP0710296A4 (de) | 1993-07-23 | 1999-05-26 | Bio Rad Laboratories | Lineare vervielfältigung von nukleinsäuren |
| CA2140081C (en) | 1994-01-13 | 2008-04-01 | Dean L. Engelhardt | Process, construct and conjugate for producing multiple nucleic acid copies |
| SE9400522D0 (sv) | 1994-02-16 | 1994-02-16 | Ulf Landegren | Method and reagent for detecting specific nucleotide sequences |
| KR100230718B1 (ko) | 1994-03-16 | 1999-11-15 | 다니엘 엘. 캐시앙, 헨리 엘. 노르호프 | 등온 가닥 변위 핵산 증폭법 |
| WO1995035390A1 (en) | 1994-06-22 | 1995-12-28 | Mount Sinai School Of Medicine Of The City University Of New York | Ligation-dependent amplification for the detection of infectious pathogens and abnormal genes |
| US5629179A (en) * | 1995-03-17 | 1997-05-13 | Novagen, Inc. | Method and kit for making CDNA library |
| WO1997019193A2 (en) | 1995-11-21 | 1997-05-29 | Yale University | Unimolecular segment amplification and detection |
| US5854033A (en) * | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| DE69636080T2 (de) | 1995-12-05 | 2006-11-30 | Erland Jorn Koch | Eine in Kaskaden verlaufende Vervielfältigungsreaktion von Nukleinsäuren |
| ATE203056T1 (de) * | 1995-12-15 | 2001-07-15 | Amersham Life Science Inc | Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität |
| GB9609213D0 (en) | 1996-05-02 | 1996-07-03 | Tepnel Medical Ltd | Amplification of nucleic acids |
| US5614390A (en) * | 1996-06-14 | 1997-03-25 | Becton, Dickinson And Company | Species-specific detection of Mycobacterium kansasii |
| US6124120A (en) * | 1997-10-08 | 2000-09-26 | Yale University | Multiple displacement amplification |
| JP4262799B2 (ja) | 1998-04-16 | 2009-05-13 | 平田機工株式会社 | 生タイヤ供給方法 |
| AU763890B2 (en) * | 1998-09-15 | 2003-07-31 | Yale University | Artificial long terminal repeat vectors |
| US6492161B1 (en) * | 1999-06-02 | 2002-12-10 | Prokaria Ltd. | Bacteriophage RM 378 of a thermophilic host organism |
| EP1057561B1 (de) * | 1999-06-04 | 2004-09-15 | André Müller | Giesskammer für eine Druckgiessmaschine |
| EP1286226B1 (de) | 2000-05-25 | 2008-02-27 | Fuji Xerox Co., Ltd. | Toner und bilderzeugungsmethode |
| EP1297004A2 (de) * | 2000-06-15 | 2003-04-02 | Prokaria ehf. | Thermostabiles cellulase |
| US20040209249A1 (en) * | 2001-02-23 | 2004-10-21 | Prokaria Ltd. | Method of obtaining protein diversity |
| US20030211494A1 (en) * | 2002-05-03 | 2003-11-13 | Prokaria, Ltd. | Retrieval of genes and gene fragments from complex samples |
| US20050282155A1 (en) * | 2004-06-17 | 2005-12-22 | Lucigen Corporation | Viral libraries from uncultivated viruses and polypeptides produced therefrom |
-
2006
- 2006-10-06 JP JP2008534757A patent/JP2009511019A/ja active Pending
- 2006-10-06 WO PCT/US2006/039406 patent/WO2007044671A2/en not_active Ceased
- 2006-10-06 EP EP11165551A patent/EP2377929A1/de not_active Withdrawn
- 2006-10-06 US US12/089,221 patent/US8093030B2/en active Active
- 2006-10-06 AT AT06836235T patent/ATE537251T1/de active
- 2006-10-06 CA CA002624324A patent/CA2624324A1/en not_active Abandoned
- 2006-10-06 EP EP06836235A patent/EP1934339B1/de active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP2377929A1 (de) | 2011-10-19 |
| WO2007044671A2 (en) | 2007-04-19 |
| US8093030B2 (en) | 2012-01-10 |
| EP1934339B1 (de) | 2011-12-14 |
| EP1934339A2 (de) | 2008-06-25 |
| CA2624324A1 (en) | 2007-04-19 |
| JP2009511019A (ja) | 2009-03-19 |
| WO2007044671A3 (en) | 2007-06-21 |
| US20080268498A1 (en) | 2008-10-30 |
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