DE69934088D1 - Verfahren zur in vitro amplifikation zirkulärer dna - Google Patents

Verfahren zur in vitro amplifikation zirkulärer dna

Info

Publication number
DE69934088D1
DE69934088D1 DE69934088T DE69934088T DE69934088D1 DE 69934088 D1 DE69934088 D1 DE 69934088D1 DE 69934088 T DE69934088 T DE 69934088T DE 69934088 T DE69934088 T DE 69934088T DE 69934088 D1 DE69934088 D1 DE 69934088D1
Authority
DE
Germany
Prior art keywords
dna
lda
amplification
circular dna
closed circular
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69934088T
Other languages
English (en)
Other versions
DE69934088T2 (de
Inventor
Zhidong Chen
E Ruffner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Utah Research Foundation UURF
Original Assignee
University of Utah Research Foundation UURF
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Utah Research Foundation UURF filed Critical University of Utah Research Foundation UURF
Publication of DE69934088D1 publication Critical patent/DE69934088D1/de
Application granted granted Critical
Publication of DE69934088T2 publication Critical patent/DE69934088T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69934088T 1998-01-09 1999-01-09 Verfahren zur in vitro amplifikation zirkulärer dna Expired - Fee Related DE69934088T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US7110198P 1998-01-09 1998-01-09
US71101P 1998-01-09
PCT/US1999/000455 WO1999035281A1 (en) 1998-01-09 1999-01-09 Method for in vitro amplification of circular dna

Publications (2)

Publication Number Publication Date
DE69934088D1 true DE69934088D1 (de) 2007-01-04
DE69934088T2 DE69934088T2 (de) 2007-06-21

Family

ID=22099270

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69934088T Expired - Fee Related DE69934088T2 (de) 1998-01-09 1999-01-09 Verfahren zur in vitro amplifikation zirkulärer dna

Country Status (6)

Country Link
US (1) US6620597B1 (de)
EP (1) EP1044281B1 (de)
AT (1) ATE346158T1 (de)
CA (1) CA2317896C (de)
DE (1) DE69934088T2 (de)
WO (1) WO1999035281A1 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6420144B1 (en) 2000-06-28 2002-07-16 Salus Therapeutics, Inc. Method for automated molecular cloning
FR2813314B1 (fr) * 2000-08-25 2004-05-07 Biomethodes Procede de mutagenese dirigee massive
US20020155439A1 (en) * 2000-12-04 2002-10-24 Ana Rodriguez Method for generating a library of mutant oligonucleotides using the linear cyclic amplification reaction
WO2003025118A2 (en) 2001-07-26 2003-03-27 Stratagene Multi-site mutagenesis
US7122715B2 (en) * 2001-09-20 2006-10-17 Monsanto Technology Llc In vitro method to create circular molecules for use in transformation
US20050244847A1 (en) * 2003-11-26 2005-11-03 Eppendorf Ag Methods and compositions for in vitro amplification of extrachromosomal nucleic acid
GB0500417D0 (en) * 2005-01-10 2005-02-16 Cambridge Antibody Tech Method of mutagenesis
GB2421950B (en) * 2005-01-10 2007-03-21 Cambridge Antibody Tech Method of mutagenesis
US8206909B2 (en) * 2006-10-05 2012-06-26 Louay K Hallak Unrestricted mutagenesis and cloning methods
WO2008141514A1 (en) * 2007-05-17 2008-11-27 Wondergen Bio-Medicine Technology Co., Ltd. System substitute pcr
GB0901593D0 (en) 2009-01-30 2009-03-11 Touchlight Genetics Ltd Production of closed linear DNA
US20110195457A1 (en) * 2010-02-09 2011-08-11 General Electric Company Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof
GB201013153D0 (en) 2010-08-04 2010-09-22 Touchlight Genetics Ltd Primer for production of closed linear DNA
US10450595B2 (en) 2013-03-15 2019-10-22 Theranos Ip Company, Llc Nucleic acid amplification
MX365323B (es) 2013-03-15 2019-05-29 Theranos Ip Co Llc Amplificación de ácidos nucléicos.
US20140302504A1 (en) * 2013-03-15 2014-10-09 Theranos, Inc. Nucleic Acid Amplification
EP2970961B1 (de) 2013-03-15 2019-04-24 Theranos IP Company, LLC Nukleinsäureamplifikation
JP2016537009A (ja) 2013-09-06 2016-12-01 セラノス， インコーポレイテッド 感染症の検出のためのシステム及び方法
WO2016187583A1 (en) * 2015-05-21 2016-11-24 Cofactor Genomics, Inc. Methods for generating circular dna from circular rna
JP2021526792A (ja) 2018-06-06 2021-10-11 マサチューセッツ インスティテュート オブ テクノロジー 真核細胞における翻訳のための環状ｒｎａ
SG11202112922WA (en) 2019-05-22 2021-12-30 Massachusetts Inst Technology Circular rna compositions and methods
EP3920976B1 (de) 2019-12-04 2023-07-19 Orna Therapeutics, Inc. Zirkuläre rns-zusammensetzungen und verfahren

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5561058A (en) * 1986-08-22 1996-10-01 Hoffmann-La Roche Inc. Methods for coupled high temperatures reverse transcription and polymerase chain reactions
US5354656A (en) * 1989-10-02 1994-10-11 Stratagene Method of DNA sequencing
KR950013953B1 (ko) 1990-01-26 1995-11-18 애보트 래보라토리즈 리가제 연쇄 반응에 적용가능한 표적 핵산의 증폭 방법
US5545552A (en) 1990-12-03 1996-08-13 Stratagene Purified thermostable pyrococcus furiosus DNA polymerase I
US5514568A (en) * 1991-04-26 1996-05-07 Eli Lilly And Company Enzymatic inverse polymerase chain reaction
WO1993012257A1 (en) 1991-12-12 1993-06-24 Hybritech Incorporated Enzymatic inverse polymerase chain reaction library mutagenesis
US5658751A (en) 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5523221A (en) * 1993-06-16 1996-06-04 Stratagene Method for the directional cloning of DNA
US5789166A (en) * 1995-12-08 1998-08-04 Stratagene Circular site-directed mutagenesis

Also Published As

Publication number Publication date
WO1999035281A1 (en) 1999-07-15
EP1044281A1 (de) 2000-10-18
CA2317896C (en) 2004-08-10
EP1044281A4 (de) 2002-07-10
US6620597B1 (en) 2003-09-16
CA2317896A1 (en) 1999-07-15
DE69934088T2 (de) 2007-06-21
EP1044281B1 (de) 2006-11-22
ATE346158T1 (de) 2006-12-15

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee