ATE346158T1 - Verfahren zur in vitro amplifikation zirkulärer dna - Google Patents
Verfahren zur in vitro amplifikation zirkulärer dnaInfo
- Publication number
- ATE346158T1 ATE346158T1 AT99901389T AT99901389T ATE346158T1 AT E346158 T1 ATE346158 T1 AT E346158T1 AT 99901389 T AT99901389 T AT 99901389T AT 99901389 T AT99901389 T AT 99901389T AT E346158 T1 ATE346158 T1 AT E346158T1
- Authority
- AT
- Austria
- Prior art keywords
- dna
- lda
- amplification
- circular dna
- closed circular
- Prior art date
Links
- 108020004638 Circular DNA Proteins 0.000 title abstract 6
- 238000000034 method Methods 0.000 title abstract 6
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 5
- 230000003321 amplification Effects 0.000 title abstract 4
- 238000000338 in vitro Methods 0.000 title abstract 2
- 108020004414 DNA Proteins 0.000 abstract 3
- 102000012410 DNA Ligases Human genes 0.000 abstract 2
- 108010061982 DNA Ligases Proteins 0.000 abstract 2
- 230000008836 DNA modification Effects 0.000 abstract 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 1
- 102000003960 Ligases Human genes 0.000 abstract 1
- 108090000364 Ligases Proteins 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 210000004671 cell-free system Anatomy 0.000 abstract 1
- 238000010367 cloning Methods 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000001415 gene therapy Methods 0.000 abstract 1
- 238000001727 in vivo Methods 0.000 abstract 1
- 231100000350 mutagenesis Toxicity 0.000 abstract 1
- 238000002703 mutagenesis Methods 0.000 abstract 1
- 231100000219 mutagenic Toxicity 0.000 abstract 1
- 230000003505 mutagenic effect Effects 0.000 abstract 1
- 230000035772 mutation Effects 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US7110198P | 1998-01-09 | 1998-01-09 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE346158T1 true ATE346158T1 (de) | 2006-12-15 |
Family
ID=22099270
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT99901389T ATE346158T1 (de) | 1998-01-09 | 1999-01-09 | Verfahren zur in vitro amplifikation zirkulärer dna |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US6620597B1 (de) |
| EP (1) | EP1044281B1 (de) |
| AT (1) | ATE346158T1 (de) |
| CA (1) | CA2317896C (de) |
| DE (1) | DE69934088T2 (de) |
| WO (1) | WO1999035281A1 (de) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6420144B1 (en) | 2000-06-28 | 2002-07-16 | Salus Therapeutics, Inc. | Method for automated molecular cloning |
| FR2813314B1 (fr) * | 2000-08-25 | 2004-05-07 | Biomethodes | Procede de mutagenese dirigee massive |
| US20020155439A1 (en) * | 2000-12-04 | 2002-10-24 | Ana Rodriguez | Method for generating a library of mutant oligonucleotides using the linear cyclic amplification reaction |
| ATE432981T1 (de) * | 2001-07-26 | 2009-06-15 | Stratagene California | Multi-stellen-mutagenese |
| US7122715B2 (en) * | 2001-09-20 | 2006-10-17 | Monsanto Technology Llc | In vitro method to create circular molecules for use in transformation |
| WO2005054435A2 (en) * | 2003-11-26 | 2005-06-16 | Eppendorf Ag | Methods and compositions for in vitro amplification of extrachromosomal nucleic acid |
| GB0500417D0 (en) * | 2005-01-10 | 2005-02-16 | Cambridge Antibody Tech | Method of mutagenesis |
| EP1844144B1 (de) * | 2005-01-10 | 2010-04-14 | MedImmune Limited | Mutageneseverfahren |
| WO2008067035A2 (en) * | 2006-10-05 | 2008-06-05 | Nationwide Children's Hospital | Unrestricted mutagenesis and cloning methods |
| WO2008141514A1 (en) * | 2007-05-17 | 2008-11-27 | Wondergen Bio-Medicine Technology Co., Ltd. | System substitute pcr |
| GB0901593D0 (en) | 2009-01-30 | 2009-03-11 | Touchlight Genetics Ltd | Production of closed linear DNA |
| US20110195457A1 (en) * | 2010-02-09 | 2011-08-11 | General Electric Company | Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof |
| GB201013153D0 (en) | 2010-08-04 | 2010-09-22 | Touchlight Genetics Ltd | Primer for production of closed linear DNA |
| US10450595B2 (en) | 2013-03-15 | 2019-10-22 | Theranos Ip Company, Llc | Nucleic acid amplification |
| AU2014233150A1 (en) | 2013-03-15 | 2015-09-17 | Theranos Ip Company, Llc | Nucleic acid amplification |
| CA2903874C (en) * | 2013-03-15 | 2024-02-20 | Theranos, Inc. | Nucleic acid amplification |
| WO2014145291A1 (en) | 2013-03-15 | 2014-09-18 | Theranos, Inc. | Nucleic acid amplification |
| EP3674422A3 (de) | 2013-09-06 | 2020-09-16 | Labrador Diagnostics LLC | Systeme und verfahren zum nachweis von infektionskrankheiten |
| HK1252166A1 (zh) * | 2015-05-21 | 2019-05-17 | 辅助因子基因组学公司 | 从环状rna产生环状dna的方法 |
| EP3801639A1 (de) | 2018-06-06 | 2021-04-14 | Massachusetts Institute of Technology | Zirkuläre rna zur translation in eukaryotischen zellen |
| AU2020280105A1 (en) | 2019-05-22 | 2022-01-20 | Massachusetts Institute Of Technology | Circular RNA compositions and methods |
| JP2023504568A (ja) | 2019-12-04 | 2023-02-03 | オルナ セラピューティクス インコーポレイテッド | 環状ｒｎａ組成物及び方法 |
| US12297285B2 (en) | 2022-06-24 | 2025-05-13 | Orna Therapeutics, Inc. | Circular RNA encoding chimeric antigen receptors targeting BCMA |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5561058A (en) * | 1986-08-22 | 1996-10-01 | Hoffmann-La Roche Inc. | Methods for coupled high temperatures reverse transcription and polymerase chain reactions |
| US5354656A (en) * | 1989-10-02 | 1994-10-11 | Stratagene | Method of DNA sequencing |
| ATE137269T1 (de) * | 1990-01-26 | 1996-05-15 | Abbott Lab | Verbessertes verfahren zur amplifikation von nuklein säurezielsequenz, einsetzbar für die polymerase und ligasekettenreaktion |
| US5545552A (en) | 1990-12-03 | 1996-08-13 | Stratagene | Purified thermostable pyrococcus furiosus DNA polymerase I |
| US5514568A (en) | 1991-04-26 | 1996-05-07 | Eli Lilly And Company | Enzymatic inverse polymerase chain reaction |
| WO1993012257A1 (en) * | 1991-12-12 | 1993-06-24 | Hybritech Incorporated | Enzymatic inverse polymerase chain reaction library mutagenesis |
| US5658751A (en) * | 1993-04-13 | 1997-08-19 | Molecular Probes, Inc. | Substituted unsymmetrical cyanine dyes with selected permeability |
| US5523221A (en) * | 1993-06-16 | 1996-06-04 | Stratagene | Method for the directional cloning of DNA |
| US5789166A (en) * | 1995-12-08 | 1998-08-04 | Stratagene | Circular site-directed mutagenesis |
-
1999
- 1999-01-09 AT AT99901389T patent/ATE346158T1/de not_active IP Right Cessation
- 1999-01-09 DE DE69934088T patent/DE69934088T2/de not_active Expired - Fee Related
- 1999-01-09 CA CA002317896A patent/CA2317896C/en not_active Expired - Fee Related
- 1999-01-09 EP EP99901389A patent/EP1044281B1/de not_active Expired - Lifetime
- 1999-01-09 US US09/582,906 patent/US6620597B1/en not_active Expired - Fee Related
- 1999-01-09 WO PCT/US1999/000455 patent/WO1999035281A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| DE69934088D1 (de) | 2007-01-04 |
| CA2317896A1 (en) | 1999-07-15 |
| US6620597B1 (en) | 2003-09-16 |
| EP1044281A1 (de) | 2000-10-18 |
| CA2317896C (en) | 2004-08-10 |
| EP1044281A4 (de) | 2002-07-10 |
| EP1044281B1 (de) | 2006-11-22 |
| DE69934088T2 (de) | 2007-06-21 |
| WO1999035281A1 (en) | 1999-07-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE346158T1 (de) | Verfahren zur in vitro amplifikation zirkulärer dna | |
| EP2272952B8 (de) | Thermostabiler oder thermoactiver DNA-polymerase mit abgeschwächter 3'-5' Exonukleaseaktivität | |
| DE60026294D1 (de) | "shot gun" sequenzierung und amplifizierung ohne klonierung | |
| NO20021223L (no) | Fremgangsmater og sammensetninger for lineaer, isoterm amplifisering av polynukleotidsekvenser | |
| BR0101493B1 (pt) | mÉtodo para transcriÇço reversa e amplificaÇço de um rna usando dna polimerases mutantes. | |
| WO2002101022A3 (en) | Method for rapid amplification of dna | |
| ATE396280T1 (de) | Detektion und amplifizierung von rna unterverwendung von zielgerichteter dna-ligation durch rna ligase | |
| ATE380883T1 (de) | Direkte multiplex charakterisierung von genomischer dna | |
| FI972992A0 (fi) | In vitro -foerfarande foer producering av sekvenseringstemplat | |
| AU6846698A (en) | Method of nucleic acid amplification | |
| AU2017265723B2 (en) | Method of amplifying circular dna | |
| DE69940662D1 (de) | Verfahren zur dna-amplifikation und -sequenzierung | |
| DE60112746D1 (de) | Kälteempfindliche dna-polymerasemutanten | |
| EP1275715A4 (de) | Verfahren zur amplifikation von nukleinsäuren unter verwendung von doppelsträngigen nukleinsäuren als schablone | |
| DE69128077D1 (de) | Nuklein-säure amplifikation mit dns abhängigen rns polymerasen aktivität von rns-replikasen | |
| WO2001061015A3 (de) | Thermostable chimeric nucleic acid polymerases and uses thereof | |
| WO2004022770A3 (en) | Compositions and methods for synthesizing nucleic acids | |
| ES2311638T3 (es) | Metodos de empleo mejorado de polimerasa. | |
| WO2002088387A3 (en) | Amplification process | |
| WO2021228905A3 (en) | Mutant polymerases and methods of using the same | |
| ATE361377T1 (de) | Leistungstarke verknüpfung von nukleinsäuresegmenten | |
| ATE440147T1 (de) | Erhíhung der spezifität von nukleinsäureamplifizierungen durch trägernukleinsäuren | |
| EP1479783A3 (de) | Methode der PCR Amplifizierung, PCR Primer Set, PCR Amplifizierungsprodukt, und PCR-Methode für die Detektion von Nukleinsäuren | |
| LT2017523A (lt) | N4-modifikuoti citidino nukleotidai ir jų panaudojimas | |
| WO2004097003A3 (en) | Multiply-primed amplification of nucleic acid sequences |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |