LT2017523A - N4-modifikuoti citidino nukleotidai ir jų panaudojimas - Google Patents

N4-modifikuoti citidino nukleotidai ir jų panaudojimas

Info

Publication number
LT2017523A
LT2017523A LT2017523A LT2017523A LT2017523A LT 2017523 A LT2017523 A LT 2017523A LT 2017523 A LT2017523 A LT 2017523A LT 2017523 A LT2017523 A LT 2017523A LT 2017523 A LT2017523 A LT 2017523A
Authority
LT
Lithuania
Prior art keywords
modified
modified cytidine
cytidine nucleotides
rna
cytidine
Prior art date
Application number
LT2017523A
Other languages
English (en)
Other versions
LT6615B (lt
Inventor
Rolandas MEŠKYS
Jevgenija JAKUBOVSKA
Daiva TAURAITĖ
Original Assignee
Vilniaus Universitetas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vilniaus Universitetas filed Critical Vilniaus Universitetas
Priority to LT2017523A priority Critical patent/LT6615B/lt
Priority to US16/646,593 priority patent/US11584772B2/en
Priority to PCT/IB2018/056961 priority patent/WO2019053609A1/en
Priority to EP18779053.0A priority patent/EP3681897B1/en
Publication of LT2017523A publication Critical patent/LT2017523A/lt
Publication of LT6615B publication Critical patent/LT6615B/lt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Šis išradimas aprašo N4-padėtyje modifikuotus citidino nukleotidus. Pateikiama N4-modifikuotų citidino trifosfatų cheminė sintezė ir aprašomi modifikuotų citidino nukleotidų panaudojimo metodai, o taip pat ir čia pateiktų ciditino analogų pritaikymas modifikuotų nukleorūgščių sintezei. Nukleorūgštys gali būti sudarytos iš DNR, RNR ar DNR/RNR kombinacijos. Viena iš modifikuotų citidino nukleotidų panaudojimo sričių yra fermentų atranka, kur fermentai pasižymi esteraziniu, amidaziniu, oksidoreduktaziniu, liaziniu, ligaziniu ar kitu fermentiniu aktyvumu.
LT2017523A 2017-09-12 2017-09-12 N4-modifikuoti citidino nukleotidai ir jų panaudojimas LT6615B (lt)

Priority Applications (4)

Application Number Priority Date Filing Date Title
LT2017523A LT6615B (lt) 2017-09-12 2017-09-12 N4-modifikuoti citidino nukleotidai ir jų panaudojimas
US16/646,593 US11584772B2 (en) 2017-09-12 2018-09-12 N4-modified cytidine nucleotides and their use
PCT/IB2018/056961 WO2019053609A1 (en) 2017-09-12 2018-09-12 CYTIDINE NUCLEOTIDES MODIFIED IN POSITION N 4 AND THEIR USE
EP18779053.0A EP3681897B1 (en) 2017-09-12 2018-09-12 N4-modified cytidine nucleotides and their use

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
LT2017523A LT6615B (lt) 2017-09-12 2017-09-12 N4-modifikuoti citidino nukleotidai ir jų panaudojimas

Publications (2)

Publication Number Publication Date
LT2017523A true LT2017523A (lt) 2019-03-25
LT6615B LT6615B (lt) 2019-04-25

Family

ID=60320948

Family Applications (1)

Application Number Title Priority Date Filing Date
LT2017523A LT6615B (lt) 2017-09-12 2017-09-12 N4-modifikuoti citidino nukleotidai ir jų panaudojimas

Country Status (4)

Country Link
US (1) US11584772B2 (lt)
EP (1) EP3681897B1 (lt)
LT (1) LT6615B (lt)
WO (1) WO2019053609A1 (lt)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112481234B (zh) * 2020-12-03 2023-09-22 重庆工业职业技术学院 一种焦磷酸酶突变体合成核酸糖类似物及其应用
CA3214286A1 (en) 2021-05-07 2022-11-10 Nikhil Dhar Modified ribonucleic acids and uses thereof
CN119080855A (zh) * 2023-06-05 2024-12-06 中国科学院深圳先进技术研究院 一种化学修饰胞嘧啶核苷三磷酸及其制备方法和应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5270163A (en) 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
US5595978A (en) 1990-08-16 1997-01-21 Isis Pharmaceuticals, Inc. Composition and method for treatment of CMV retinites
GB0022458D0 (en) 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
JP5076504B2 (ja) * 2007-01-05 2012-11-21 富士通株式会社 核酸合成用アミダイド及び核酸合成方法
WO2011028218A1 (en) * 2009-09-02 2011-03-10 Alnylam Pharmaceuticals, Inc. Process for triphosphate oligonucleotide synthesis
US20130142796A1 (en) 2011-12-05 2013-06-06 Subhransu Ray Treatment for angiogenic disorders
EP2931319B1 (en) * 2012-12-13 2019-08-21 ModernaTX, Inc. Modified nucleic acid molecules and uses thereof
US9624492B2 (en) 2013-02-13 2017-04-18 Amarin Pharmaceuticals Ireland Limited Compositions comprising eicosapentaenoic acid and mipomersen and methods of use thereof
US9771613B2 (en) * 2013-04-02 2017-09-26 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acid
WO2015051214A1 (en) * 2013-10-03 2015-04-09 Moderna Therapeutics, Inc. Polynucleotides encoding low density lipoprotein receptor
SG10202006426QA (en) 2013-11-21 2020-08-28 Somalogic Inc Cytidine-5-carboxamide modified nucleotide compositions and methods related thereto
FR3025201B1 (fr) * 2014-09-02 2018-10-12 Dna Script Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques
WO2016164762A1 (en) * 2015-04-08 2016-10-13 Moderna Therapeutics, Inc. Polynucleotides encoding low density lipoprotein receptor egf-a and intracellular domain mutants and methods of using the same

Also Published As

Publication number Publication date
LT6615B (lt) 2019-04-25
EP3681897A1 (en) 2020-07-22
EP3681897C0 (en) 2025-05-14
EP3681897B1 (en) 2025-05-14
US20200270295A1 (en) 2020-08-27
US11584772B2 (en) 2023-02-21
WO2019053609A1 (en) 2019-03-21

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Legal Events

Date Code Title Description
BB1A Patent application published

Effective date: 20190325

FG9A Patent granted

Effective date: 20190425