ES2311638T3 - Metodos de empleo mejorado de polimerasa. - Google Patents

Metodos de empleo mejorado de polimerasa. Download PDF

Info

Publication number
ES2311638T3
ES2311638T3 ES02786844T ES02786844T ES2311638T3 ES 2311638 T3 ES2311638 T3 ES 2311638T3 ES 02786844 T ES02786844 T ES 02786844T ES 02786844 T ES02786844 T ES 02786844T ES 2311638 T3 ES2311638 T3 ES 2311638T3
Authority
ES
Spain
Prior art keywords
improved polymerase
employment methods
methods improved
polymerase
employment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES02786844T
Other languages
English (en)
Other versions
ES2311638T5 (es
Inventor
Yan Wang
Horn Peter Vander
Lei Xi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Bio Rad Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23304983&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2311638(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc
Publication of ES2311638T3 publication Critical patent/ES2311638T3/es
Application granted granted Critical
Publication of ES2311638T5 publication Critical patent/ES2311638T5/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor

Abstract

Un método para incrementar el rendimiento de una reacción de polimerasa sobre un ácido nucleico diana presente en una solución que contiene un inhibidor de la polimerasa, comprendiendo el método: (a) la puesta en contacto del ácido nucleico diana con una polimerasa, donde la polimerasa está unida covalentemente a un dominio de unión a ácido nucleico no específico de ninguna secuencia que: (i) se une a ácido nucleico bicatenario y (ii) incrementa la procesividad de la polimerasa en comparación con una polimerasa idéntica que no tenga fusionado a la misma el dominio de unión a ácido nucleico no específico de ninguna secuencia, y donde la solución tiene una composición que permite al dominio de unión unirse al ácido nucleico diana y al dominio polimerasa extender un cebador que esté hibridado a la secuencia de ácido nucleico diana; (b) la incubación de la solución en condiciones bajo las cuales el cebador sea extendido por la polimerasa, con la condición de que la sal no sea un inhibidor.
ES02786844T 2001-11-28 2002-11-27 Métodos de uso de polimerasas mejoradas. Expired - Lifetime ES2311638T5 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33396601P 2001-11-28 2001-11-28
US333966P 2001-11-28

Publications (2)

Publication Number Publication Date
ES2311638T3 true ES2311638T3 (es) 2009-02-16
ES2311638T5 ES2311638T5 (es) 2011-09-08

Family

ID=23304983

Family Applications (1)

Application Number Title Priority Date Filing Date
ES02786844T Expired - Lifetime ES2311638T5 (es) 2001-11-28 2002-11-27 Métodos de uso de polimerasas mejoradas.

Country Status (10)

Country Link
US (4) US8232078B2 (es)
EP (1) EP1456394B2 (es)
JP (1) JP4689163B2 (es)
AT (1) ATE406456T1 (es)
AU (1) AU2002351198B2 (es)
CA (1) CA2468838A1 (es)
DE (1) DE60228618D1 (es)
DK (1) DK1456394T4 (es)
ES (1) ES2311638T5 (es)
WO (1) WO2003046149A2 (es)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2521127C (en) 2003-04-01 2014-05-27 Eragen Biosciences, Inc. Polymerase inhibitor and method of using same
EP1706502A4 (en) * 2003-11-26 2007-05-23 Eppendorf Ag METHODS AND COMPOSITIONS FOR IN VITRO AMPLIFICATION OF EXTRACHROMOSOMIC NUCLEIC ACID
WO2006074288A2 (en) 2005-01-06 2006-07-13 Applera Corporation Polypeptides having nucleic acid binding activity
EP2208796B1 (en) 2005-01-06 2014-06-18 Applied Biosystems, LLC Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification
WO2007050125A2 (en) * 2005-05-27 2007-05-03 William Marsh Rice University High processivity polymerases
US20070059713A1 (en) 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
JP4899618B2 (ja) * 2006-05-01 2012-03-21 ソニー株式会社 二本鎖核酸検出方法、ハイブリダイゼーション検出方法
KR100825279B1 (ko) * 2006-11-30 2008-04-25 한국해양연구원 Ｄｎα 중합효소 활성 증가 단백질 및 이를 암호화 하는유전자
US8338094B2 (en) * 2007-11-27 2012-12-25 Bio-Rad Laboratories, Inc. Reduced inhibition of one-step RT-PCR
WO2009087394A1 (en) * 2008-01-11 2009-07-16 Genesys Ltd Cren7 chimeric protein
GB0803628D0 (en) 2008-02-28 2008-04-02 Genesys Ltd Enzyme
JP5809059B2 (ja) 2008-11-03 2015-11-10 カパ バイオシステムズ， インコーポレイテッド 改変ａ型ｄｎａポリメラーゼ
EP2382321A4 (en) 2009-01-08 2012-12-19 Bio Rad Laboratories METHODS AND COMPOSITIONS FOR ENHANCING THE EFFICACY OF NUCLEIC ACID AMPLIFICATION REACTIONS
WO2010091203A2 (en) * 2009-02-04 2010-08-12 Lucigen Corporation Rna-and dna-copying enzymes
US9617588B2 (en) 2010-10-05 2017-04-11 Thermo Fisher Scientific Baltics Uab Enzyme mixture
US9315787B2 (en) 2011-01-14 2016-04-19 Kapa Biosystems, Inc. Modified DNA polymerases for improved amplification
WO2012138417A1 (en) * 2011-04-08 2012-10-11 Bio-Rad Laboratories, Inc. Sso7-polymerase conjugates with decreased non-specific activity
EP2694670B1 (en) 2011-04-08 2017-07-19 Bio-Rad Laboratories, Inc. Pcr reaction mixtures with decreased non-specific activity
US8470573B2 (en) * 2011-06-21 2013-06-25 Bio-Rad Laboratories, Inc. Hybrid polymerases having the ability to produce long amplicons
US10023856B2 (en) * 2013-09-25 2018-07-17 Thermo Fisher Scientific Baltics Uab Enzyme composition for DNA end repair, adenylation, phosphorylation
US10072285B2 (en) 2014-08-19 2018-09-11 Bio-Rad Laboratories, Inc. RNA amplification methods
US9963687B2 (en) * 2014-08-27 2018-05-08 New England Biolabs, Inc. Fusion polymerase and method for using the same
US9447445B2 (en) * 2014-08-27 2016-09-20 New England Biolabs, Inc. Synthon formation
EP3486329A1 (en) 2015-02-17 2019-05-22 Bio-Rad Laboratories, Inc. Method of generating and amplifying cdna
EP3728633A1 (en) 2017-12-22 2020-10-28 Thermo Fisher Scientific Baltics Uab Polymerase chain reaction composition comprising amines
US11168312B2 (en) * 2019-03-10 2021-11-09 Abclonal Science, Inc. Mutant taq polymerase for amplification in increased salt concentration or body fluids
US11613738B2 (en) * 2019-05-14 2023-03-28 Abclonal Science, Inc. Mutant Taq polymerase resistant to inhibition of amplification in the presence of cyanine dye
WO2023146243A1 (ko) * 2022-01-26 2023-08-03 주식회사 씨젠 샘플 내 타겟 핵산을 검출하는 방법

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5512482A (en) * 1990-04-26 1996-04-30 Calgene, Inc. Plant thioesterases
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5658751A (en) 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5512462A (en) 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US5614365A (en) 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
US5972603A (en) 1996-02-09 1999-10-26 President And Fellows Of Harvard College DNA polymerase with modified processivity
US6228628B1 (en) * 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
DE19840771A1 (de) 1998-08-06 2000-02-10 Lion Bioscience Ag Thermostabiler in vitro-Komplex mit Polymeraseaktivität
DE19937230A1 (de) * 1999-08-06 2001-02-08 Lion Bioscience Ag Chimäre Proteine
US6627424B1 (en) * 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes

Also Published As

Publication number Publication date
AU2002351198B2 (en) 2008-08-14
WO2003046149A3 (en) 2004-02-19
JP2005510235A (ja) 2005-04-21
JP4689163B2 (ja) 2011-05-25
EP1456394B1 (en) 2008-08-27
US20120295270A1 (en) 2012-11-22
US9139873B2 (en) 2015-09-22
EP1456394B2 (en) 2011-04-27
DE60228618D1 (de) 2008-10-09
US20140220577A1 (en) 2014-08-07
US8232078B2 (en) 2012-07-31
EP1456394A2 (en) 2004-09-15
US20160060616A1 (en) 2016-03-03
US8476045B2 (en) 2013-07-02
ES2311638T5 (es) 2011-09-08
CA2468838A1 (en) 2003-06-05
AU2002351198A1 (en) 2003-06-10
EP1456394A4 (en) 2005-12-28
WO2003046149A2 (en) 2003-06-05
US9708598B2 (en) 2017-07-18
ATE406456T1 (de) 2008-09-15
DK1456394T4 (da) 2011-08-15
DK1456394T3 (da) 2008-11-24
US20040002076A1 (en) 2004-01-01

Similar Documents

Publication Publication Date Title
ES2311638T3 (es) Metodos de empleo mejorado de polimerasa.
ATE368104T1 (de) Verbesserte nukleinsäure-modifizierende enzyme
DE60235491D1 (de) Paralleles scoring von polymorphismen mittels amplifikation und fehlerkorrektur
ATE557083T1 (de) Thermostabiler oder thermoactiver dna-polymerase mit abgeschwächter 3'-5' exonukleaseaktivität
DE60143632D1 (de) Thermostabile chimäre Nukleinsäurepolymerasen und ihre Verwendungen
WO2003025132A3 (en) Dna polymerases and mutants thereof
DK1427852T3 (da) Enkelnukleotidamplikation og -detektion med polymerase
NO20012561D0 (no) Termostabile enzymer som fremmer fideliteten av termostabile DNA-polymeraser for forbedring av nukleinsyresyntese ogamplifikasjon in vitro
DE60122962D1 (de) Reverse Transkription bei hoher Temperatur durch die Verwendung von mutierten DNA Polymerasen
ATE432365T1 (de) Verfahren, kits und reagenzien zur nukleinsäuresequenzierung
ATE325877T1 (de) Mutierte chimäre dns-polymerasen
EP1091002A3 (en) Sequential activation of one or more enzymatic activities within a thermocycling reaction for synthesizing DNA molecules
WO2005083068A3 (en) Methods for obtaining thermostable enzymes, dna polymerase i variants from thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications to the same
WO2008051530A3 (en) Polymerase enzymes and reagents for enhanced nucleic acid sequencing
WO2006074217A3 (en) Hot start polymerase reaction using a thermolabile blocker
ATE427361T1 (de) Verfahren zur dna-amplifikation und - sequenzierung
DE69934088D1 (de) Verfahren zur in vitro amplifikation zirkulärer dna
ATE330031T1 (de) Reversibel inhibierende sonden
WO2001064838A3 (en) Thermophilic dna polymerases from thermoactinomyces vulgaris
US10358673B2 (en) Method of amplifying nucleic acid sequences
DE602006018701D1 (de) Polypeptide mit nukleinsäurebindender aktivität
ATE455866T1 (de) Verfahren zur unspezifischen amplifizierung nukleinsäuren mit niedriger komplexität
ATE534731T1 (de) Zusammensetzungen und verfahren zur zufallsmutagenese von nukleinsäuren
ATE311477T1 (de) Hohe dichte markierung von dns mit modifizierte oder chromophore tragende nukleotide und benutzte dns polymerasen
AU2001242128A1 (en) Dna joining method