AU2001242128A1 - Dna joining method - Google Patents

Dna joining method

Info

Publication number
AU2001242128A1
AU2001242128A1 AU2001242128A AU4212801A AU2001242128A1 AU 2001242128 A1 AU2001242128 A1 AU 2001242128A1 AU 2001242128 A AU2001242128 A AU 2001242128A AU 4212801 A AU4212801 A AU 4212801A AU 2001242128 A1 AU2001242128 A1 AU 2001242128A1
Authority
AU
Australia
Prior art keywords
joining method
linear
vector
dna
dna joining
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001242128A
Inventor
David H. Evans
David O. Willer
Xiao-Dan Yao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Guelph
Original Assignee
University of Guelph
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Guelph filed Critical University of Guelph
Publication of AU2001242128A1 publication Critical patent/AU2001242128A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

Abstract

The present invention provides a method to directionally clone any linear template DNA molecule into any linearized vector. The vector ends may be generated from any restriction enzyme cleavage. The method does not require a ligation step nor the use of carefully controlled conditions as is required with methods involving specific exonucleases alone. It has been determined that specific DNA polymerases are able to efficiently join one or more linear DNA molecules sharing ends with appropriate complementation.
AU2001242128A 2000-03-07 2001-03-07 Dna joining method Abandoned AU2001242128A1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US18740100P 2000-03-07 2000-03-07
US60187401 2000-03-07
CA002317865A CA2317865A1 (en) 2000-03-07 2000-09-01 Dna joining method
CA2317865 2000-09-01
US26377101P 2001-01-25 2001-01-25
US60263771 2001-01-25
PCT/CA2001/000283 WO2001066775A2 (en) 2000-03-07 2001-03-07 Dna joining method

Publications (1)

Publication Number Publication Date
AU2001242128A1 true AU2001242128A1 (en) 2001-09-17

Family

ID=22688823

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001242128A Abandoned AU2001242128A1 (en) 2000-03-07 2001-03-07 Dna joining method

Country Status (7)

Country Link
EP (1) EP1263973B1 (en)
JP (1) JP4854160B2 (en)
AT (1) ATE335827T1 (en)
AU (1) AU2001242128A1 (en)
CA (1) CA2317865A1 (en)
DE (1) DE60122118T2 (en)
WO (1) WO2001066775A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MY143596A (en) 2005-08-11 2011-06-15 Synthetic Genomics Inc In vitro recombination method
WO2007120624A2 (en) * 2006-04-10 2007-10-25 Codon Devices, Inc. Concerted nucleic acid assembly reactions
WO2010059763A1 (en) 2008-11-19 2010-05-27 Amyris Biotechnologies, Inc. Compositions and methods for the assembly of polynucleotides
US9963687B2 (en) 2014-08-27 2018-05-08 New England Biolabs, Inc. Fusion polymerase and method for using the same
EP3450558B1 (en) * 2014-08-27 2020-09-30 New England Biolabs, Inc. Synthon formation

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5646019A (en) * 1989-10-24 1997-07-08 Stratagene Method for producing primed nucleic acid templates
US5580759A (en) * 1994-02-03 1996-12-03 Board Of Regents, The University Of Texas System Construction of recombinant DNA by exonuclease recession
IL120338A0 (en) * 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Single step DNA fragments assembly

Also Published As

Publication number Publication date
EP1263973A2 (en) 2002-12-11
CA2317865A1 (en) 2001-09-07
WO2001066775A2 (en) 2001-09-13
ATE335827T1 (en) 2006-09-15
WO2001066775A3 (en) 2002-03-28
EP1263973B1 (en) 2006-08-09
DE60122118D1 (en) 2006-09-21
DE60122118T2 (en) 2007-03-08
JP4854160B2 (en) 2012-01-18
JP2003533979A (en) 2003-11-18

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