AU2001237425A1 - Rna polymerase mutants with increased thermostability - Google Patents

Rna polymerase mutants with increased thermostability

Info

Publication number
AU2001237425A1
AU2001237425A1 AU2001237425A AU3742501A AU2001237425A1 AU 2001237425 A1 AU2001237425 A1 AU 2001237425A1 AU 2001237425 A AU2001237425 A AU 2001237425A AU 3742501 A AU3742501 A AU 3742501A AU 2001237425 A1 AU2001237425 A1 AU 2001237425A1
Authority
AU
Australia
Prior art keywords
rna polymerase
increased thermostability
polymerase mutants
rna polymerases
bacteriophages
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001237425A
Inventor
Bunsei Kawakami
Yoshiaki Nishiya
Akio Sugiyama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Akzo Nobel NV
Original Assignee
Akzo Nobel NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Akzo Nobel NV filed Critical Akzo Nobel NV
Publication of AU2001237425A1 publication Critical patent/AU2001237425A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Heat Sensitive Colour Forming Recording (AREA)

Abstract

The present application relates to mutated RNA polymerases from bacteriophages that have increased stability, for example under high temperature conditions. Preferred mutated RNA polymerases according to the invention are mutant RNA polymerases from T7 or SP3 bacteriophages. An especially preferred embodiment of the present invention is a T7 RNA polymerase with a serine to proline amino acid change in the protein at position 633 of the amino acid sequence.
AU2001237425A 2000-03-07 2001-03-01 Rna polymerase mutants with increased thermostability Abandoned AU2001237425A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP00200787 2000-03-07
EP00200787 2000-03-07
PCT/EP2001/002327 WO2001066705A1 (en) 2000-03-07 2001-03-01 Rna polymerase mutants with increased thermostability

Publications (1)

Publication Number Publication Date
AU2001237425A1 true AU2001237425A1 (en) 2001-09-17

Family

ID=8171152

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001237425A Abandoned AU2001237425A1 (en) 2000-03-07 2001-03-01 Rna polymerase mutants with increased thermostability

Country Status (9)

Country Link
US (1) US7507567B2 (en)
EP (1) EP1261696B1 (en)
JP (1) JP4832694B2 (en)
AT (1) ATE294855T1 (en)
AU (1) AU2001237425A1 (en)
CA (1) CA2400049C (en)
DE (1) DE60110564T2 (en)
ES (1) ES2242733T3 (en)
WO (1) WO2001066705A1 (en)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009136153A (en) * 2007-12-03 2009-06-25 Tosoh Corp Variant rna polymerase
WO2009113718A1 (en) * 2008-03-11 2009-09-17 東ソー株式会社 Rna polymerase mutant having improved functions
EP2316934B1 (en) * 2008-08-08 2015-05-13 Tosoh Corporation Rna polymerase mutant with improved functions
US9193959B2 (en) 2010-04-16 2015-11-24 Roche Diagnostics Operations, Inc. T7 RNA polymerase variants with enhanced thermostability
EP3219796B1 (en) 2010-08-31 2020-10-07 GreenLight Biosciences, Inc. Methods for control of flux in metabolic pathways through protease manipulation
US9062292B2 (en) 2010-09-13 2015-06-23 Enzo Life Sciences Inc. Mutant T7 polymerases
FR2981088B1 (en) 2011-10-06 2013-11-29 Biomerieux Sa RNA POLYMERASES MUTEES
US9045740B2 (en) * 2012-02-24 2015-06-02 University Of Massachusetts Modified T7-related RNA polymerases and methods of use thereof
US9688977B2 (en) 2013-08-05 2017-06-27 Greenlight Biosciences, Inc. Engineered phosphoglucose isomerase proteins with a protease cleavage site
WO2015024017A2 (en) * 2013-08-16 2015-02-19 President And Fellows Of Harvard College Rna polymerase, methods of purification and methods of use
WO2015143318A1 (en) * 2014-03-20 2015-09-24 The Board Of Regents Of The University Of Texas System T7 rna polymerase variants with expanded substrate range and enhanced transcriptional yield
MY193444A (en) 2015-03-30 2022-10-13 Greenlight Biosciences Inc Cell-free production of ribonucleic acid
WO2017001058A1 (en) * 2015-07-01 2017-01-05 Curevac Ag Method for analysis of an rna molecule
CN108779446B (en) * 2016-01-13 2023-04-21 新英格兰生物实验室公司 Thermostable variants of T7 RNA polymerase
CR20180525A (en) 2016-04-06 2019-02-14 Greenlight Biosciences Inc RIBONUCLEIC ACID PRODUCTION FREE OF CELLS
US10034951B1 (en) * 2017-06-21 2018-07-31 New England Biolabs, Inc. Use of thermostable RNA polymerases to produce RNAs having reduced immunogenicity
US10793841B2 (en) 2017-06-30 2020-10-06 Codexis, Inc. T7 RNA polymerase variants
JP2020530267A (en) 2017-06-30 2020-10-22 コデクシス， インコーポレイテッド T7 RNA polymerase variant
KR102571743B1 (en) 2017-10-11 2023-08-29 그린라이트 바이오사이언시스, 아이엔씨. Methods and compositions for the production of nucleoside triphosphates and ribonucleic acids
JP2021520815A (en) * 2018-04-10 2021-08-26 グリーンライト バイオサイエンシーズ インコーポレーテッドＧｒｅｅｎｌｉｇｈｔ Ｂｉｏｓｃｉｅｎｃｅｓ，Ｉｎｃ． T7 RNA polymerase variant
WO2020243026A1 (en) * 2019-05-24 2020-12-03 Primordial Genetics Inc. Methods and compositions for manufacturing polynucleotides
CN112980811B (en) * 2021-03-06 2022-02-25 苏州瀚源新酶生物科技有限公司 RNA polymerase mutant and application thereof, recombinant vector and preparation method and application thereof, recombinant engineering bacteria and application thereof
EP4108770A1 (en) 2021-06-24 2022-12-28 Aptamimetics GmbH Age-selex method for aptamer identification and use

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4952496A (en) 1984-03-30 1990-08-28 Associated Universities, Inc. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5385834A (en) * 1993-08-13 1995-01-31 Georgia Tech Research Corporation Mutant T7 RNA polymerase GP1(lys222) exhibiting altered promoter recognition
FR2761695B1 (en) 1997-04-04 1999-09-24 Bio Merieux RNA POLYMERASE PREFERENTIALLY FUNCTIONING ON RNA MATRIX, ITS PREPARATION AND ITS USE
JP3172710B2 (en) 1997-07-07 2001-06-04 理化学研究所 RNA polymerase
DE69917322T2 (en) * 1998-12-11 2005-05-04 bioMérieux B.V. RNA POLYMERASE MUTANTS WITH INCREASED STABILITY
JP4399684B2 (en) * 1999-08-17 2010-01-20 東洋紡績株式会社 Improved RNA polymerase

Also Published As

Publication number Publication date
US7507567B2 (en) 2009-03-24
WO2001066705A1 (en) 2001-09-13
DE60110564T2 (en) 2006-01-19
US20030175738A1 (en) 2003-09-18
ES2242733T3 (en) 2005-11-16
DE60110564D1 (en) 2005-06-09
CA2400049A1 (en) 2001-09-13
JP2003525627A (en) 2003-09-02
CA2400049C (en) 2010-10-05
EP1261696A1 (en) 2002-12-04
EP1261696B1 (en) 2005-05-04
ATE294855T1 (en) 2005-05-15
JP4832694B2 (en) 2011-12-07

Similar Documents

Publication Publication Date Title
AU2001237425A1 (en) Rna polymerase mutants with increased thermostability
ES2162132T3 (en) THERMO-STABLE DNA POLYMERASE FROM THERMOANAEROBACTER THERMOHYDROSULFURICUS AND MUTANT ENZYMES, OF SUPPLIED EXONUCLEASE ACTIVITY SO OBTAINED.
WO2001051621A3 (en) Dna polymerase mutant having one or more mutations in the active site
AU1239901A (en) Compositions and methods utilizing dna polymerases
CA2251643A1 (en) Synthesis of fluorophore-labeled dna
DE69941701D1 (en) MUTANTS OF FLT3-L AND ITS USES
AU691374B2 (en) 5' to 3' exonuclease mutations of thermostable DNA polymerases
PT1165763E (en) VARIABLES OF HYDANTOINASE WITH IMPROVED PROPERTIES AND ITS USE FOR THE PRODUCTION OF AMINO ACIDS
EP1507002A3 (en) Thermostable Taq polymerase fragment
HUP0103601A2 (en) Hybrid protein for inhibiting the degranulation of mastocytes and the use thereof
MXPA03002572A (en) Nucleotide sequences mediating male fertility and method of using same.
FI934812A0 (en) STABILIZER ENTZYMER
HUP0203259A2 (en) Site-directed mutagenesis of escherichia coli phytase
AU1975200A (en) Mutants of rna polymerases with increased stability
CA2170515A1 (en) Nucleotide sequences for novel protein tyrosine phosphatases
WO2003048309A3 (en) Thermus thermophilus nucleic acid polymerases
WO2007058267A8 (en) Novel protein and gene encoding the protein
EP1064296A4 (en) Thermostable dna polymerase from thermoanaerobacter thermohydrosulfuricus
MXPA02010868A (en) Nucleotide sequences involved in increasing or decreasing mammalian ovulation rate.
AU2002211843A1 (en) 2.5-diketo-d-gluconic acid reductases and methods of use
GT200000138CA (en) GENE OF STEPTOMYCES AVERMITILIS THAT DIRECTS THE RELATIONSHIP OF AVERMECTINES B1: B2 (FRACTIONARY APPLICATION NO.3, DERIVED FROM LAPATENT PI-2000-0138)
NZ504633A (en) A dna molecule encoding a mutant prepro-neuropeptide y, a mutant signal peptide, and uses thereof
HUP0400150A2 (en) Saponin-digesting enzymes, genes thereof and soyasapogenol b mass production system
AU4113897A (en) Drosophila melanogaster p70 s6 kinase
WO2003100075A3 (en) Bsnd nucleic acids and proteins