JP4899618B2 - 二本鎖核酸検出方法、ハイブリダイゼーション検出方法 - Google Patents
二本鎖核酸検出方法、ハイブリダイゼーション検出方法 Download PDFInfo
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- JP4899618B2 JP4899618B2 JP2006127852A JP2006127852A JP4899618B2 JP 4899618 B2 JP4899618 B2 JP 4899618B2 JP 2006127852 A JP2006127852 A JP 2006127852A JP 2006127852 A JP2006127852 A JP 2006127852A JP 4899618 B2 JP4899618 B2 JP 4899618B2
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Description
本発明に係る二本鎖核酸検出方法について、以下説明する。
本発明に係る二本鎖核酸検出方法は、所定の検出表面を用いたハイブリダイゼーション検出などにも適用できる。
Claims (2)
- インターカレーターとしてSYBR(登録商標)Green I(商品名)を用いる二本鎖核酸検出方法であって、
リン酸バッファー存在下、前記二本鎖核酸1mer当たり0.109μM以上0.140μM以下の前記SYBR(登録商標)Green I(商品名)を反応場に投入する二本鎖核酸検出方法。 - インターカレーターとしてSYBR(登録商標)Green I(商品名)を用いて、検出表面に固定された検出用の核酸プローブと標的核酸とのハイブリダイゼーションを検出するハイブリダイゼーション検出方法であって、
リン酸バッファー存在下、前記二本鎖核酸1mer当たり0.109μM以上0.140μM以下の前記SYBR(登録商標)Green I(商品名)を前記検出表面に臨む反応場に投入するハイブリダイゼーション検出方法。
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JP2006127852A JP4899618B2 (ja) | 2006-05-01 | 2006-05-01 | 二本鎖核酸検出方法、ハイブリダイゼーション検出方法 |
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JP2006127852A JP4899618B2 (ja) | 2006-05-01 | 2006-05-01 | 二本鎖核酸検出方法、ハイブリダイゼーション検出方法 |
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JP2007295870A JP2007295870A (ja) | 2007-11-15 |
JP4899618B2 true JP4899618B2 (ja) | 2012-03-21 |
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JP2006127852A Expired - Fee Related JP4899618B2 (ja) | 2006-05-01 | 2006-05-01 | 二本鎖核酸検出方法、ハイブリダイゼーション検出方法 |
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EP1456394B2 (en) * | 2001-11-28 | 2011-04-27 | Bio-Rad Laboratories, Inc. | Methods of using improved polymerases |
ES2438967T3 (es) * | 2002-10-23 | 2014-01-21 | University Of Utah Research Foundation | Análisis de fusión de amplicones con colorantes de saturación |
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