JP2020524493A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2020524493A5 JP2020524493A5 JP2019567368A JP2019567368A JP2020524493A5 JP 2020524493 A5 JP2020524493 A5 JP 2020524493A5 JP 2019567368 A JP2019567368 A JP 2019567368A JP 2019567368 A JP2019567368 A JP 2019567368A JP 2020524493 A5 JP2020524493 A5 JP 2020524493A5
- Authority
- JP
- Japan
- Prior art keywords
- saccharoporyspora
- strain
- library
- item
- htp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims description 225
- 230000007614 genetic variation Effects 0.000 claims description 118
- VGGSULWDCMWZPO-ODEMIOGVSA-N spinosin Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=2C(=O)C=C(OC=2C=C1OC)C=1C=CC(O)=CC=1)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O VGGSULWDCMWZPO-ODEMIOGVSA-N 0.000 claims description 106
- 108090000623 proteins and genes Proteins 0.000 claims description 91
- 210000004027 cell Anatomy 0.000 claims description 84
- 230000000813 microbial effect Effects 0.000 claims description 63
- VGGSULWDCMWZPO-UHFFFAOYSA-N flavoayamenin Natural products COC1=CC=2OC(C=3C=CC(O)=CC=3)=CC(=O)C=2C(O)=C1C1OC(CO)C(O)C(O)C1OC1OC(CO)C(O)C(O)C1O VGGSULWDCMWZPO-UHFFFAOYSA-N 0.000 claims description 53
- 238000004519 manufacturing process Methods 0.000 claims description 43
- 230000037361 pathway Effects 0.000 claims description 35
- 230000000340 anti-metabolite Effects 0.000 claims description 29
- 239000002256 antimetabolite Substances 0.000 claims description 29
- 229940100197 antimetabolite Drugs 0.000 claims description 29
- 239000013612 plasmid Substances 0.000 claims description 29
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 claims description 28
- 239000003550 marker Substances 0.000 claims description 28
- 238000002703 mutagenesis Methods 0.000 claims description 24
- 231100000350 mutagenesis Toxicity 0.000 claims description 24
- 239000012634 fragment Substances 0.000 claims description 23
- 244000005700 microbiome Species 0.000 claims description 22
- 238000013461 design Methods 0.000 claims description 21
- 150000001413 amino acids Chemical class 0.000 claims description 18
- 239000002207 metabolite Substances 0.000 claims description 18
- 150000001875 compounds Chemical class 0.000 claims description 17
- ZYVMPHJZWXIFDQ-LURJTMIESA-N alpha-methylmethionine Chemical compound CSCC[C@](C)(N)C(O)=O ZYVMPHJZWXIFDQ-LURJTMIESA-N 0.000 claims description 16
- 239000002773 nucleotide Substances 0.000 claims description 16
- 125000003729 nucleotide group Chemical group 0.000 claims description 16
- 238000012216 screening Methods 0.000 claims description 16
- ULGZDMOVFRHVEP-RWJQBGPGSA-N Erythromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 ULGZDMOVFRHVEP-RWJQBGPGSA-N 0.000 claims description 14
- 210000001938 protoplast Anatomy 0.000 claims description 13
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 claims description 12
- 238000003780 insertion Methods 0.000 claims description 12
- 230000037431 insertion Effects 0.000 claims description 12
- ZSLZBFCDCINBPY-ZSJPKINUSA-N acetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZSLZBFCDCINBPY-ZSJPKINUSA-N 0.000 claims description 10
- 238000012217 deletion Methods 0.000 claims description 10
- 230000037430 deletion Effects 0.000 claims description 10
- LGVJIYCMHMKTPB-BKLSDQPFSA-N (2s)-2-amino-3-hydroxypentanoic acid Chemical compound CCC(O)[C@H](N)C(O)=O LGVJIYCMHMKTPB-BKLSDQPFSA-N 0.000 claims description 9
- 229940024606 amino acid Drugs 0.000 claims description 9
- -1 Cerlenin Chemical compound 0.000 claims description 8
- 102000004190 Enzymes Human genes 0.000 claims description 7
- 108090000790 Enzymes Proteins 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 7
- 229940121710 HMGCoA reductase inhibitor Drugs 0.000 claims description 7
- 230000015572 biosynthetic process Effects 0.000 claims description 7
- 150000001732 carboxylic acid derivatives Chemical class 0.000 claims description 7
- 229960003276 erythromycin Drugs 0.000 claims description 7
- 239000000446 fuel Substances 0.000 claims description 7
- 239000002471 hydroxymethylglutaryl coenzyme A reductase inhibitor Substances 0.000 claims description 7
- 230000010354 integration Effects 0.000 claims description 7
- 230000003834 intracellular effect Effects 0.000 claims description 7
- 150000007524 organic acids Chemical class 0.000 claims description 7
- 102000054765 polymorphisms of proteins Human genes 0.000 claims description 7
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 7
- 150000003384 small molecules Chemical class 0.000 claims description 7
- BFWPTYMTWQBGHH-RUDMXATFSA-N spinosin a Chemical compound COC1=C(O)C(OC)=CC(\C=C\C(=O)OCC2C(C(O)C(O)C(OC3C(OC(CO)C(O)C3O)C=3C(=C4C(=O)C=C(OC4=CC=3OC)C=3C=CC(O)=CC=3)O)O2)O)=C1 BFWPTYMTWQBGHH-RUDMXATFSA-N 0.000 claims description 7
- 238000003786 synthesis reaction Methods 0.000 claims description 7
- ODFFPRGJZRXNHZ-UHFFFAOYSA-N 5-fluoroindole Chemical compound FC1=CC=C2NC=CC2=C1 ODFFPRGJZRXNHZ-UHFFFAOYSA-N 0.000 claims description 6
- TWCMVXMQHSVIOJ-UHFFFAOYSA-N Aglycone of yadanzioside D Natural products COC(=O)C12OCC34C(CC5C(=CC(O)C(O)C5(C)C3C(O)C1O)C)OC(=O)C(OC(=O)C)C24 TWCMVXMQHSVIOJ-UHFFFAOYSA-N 0.000 claims description 6
- PLMKQQMDOMTZGG-UHFFFAOYSA-N Astrantiagenin E-methylester Natural products CC12CCC(O)C(C)(CO)C1CCC1(C)C2CC=C2C3CC(C)(C)CCC3(C(=O)OC)CCC21C PLMKQQMDOMTZGG-UHFFFAOYSA-N 0.000 claims description 6
- PFOARMALXZGCHY-UHFFFAOYSA-N homoegonol Natural products C1=C(OC)C(OC)=CC=C1C1=CC2=CC(CCCO)=CC(OC)=C2O1 PFOARMALXZGCHY-UHFFFAOYSA-N 0.000 claims description 6
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims description 5
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims description 5
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims description 5
- 239000004473 Threonine Substances 0.000 claims description 5
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims description 5
- 229930182817 methionine Natural products 0.000 claims description 5
- AGNGYMCLFWQVGX-AGFFZDDWSA-N (e)-1-[(2s)-2-amino-2-carboxyethoxy]-2-diazonioethenolate Chemical compound OC(=O)[C@@H](N)CO\C([O-])=C\[N+]#N AGNGYMCLFWQVGX-AGFFZDDWSA-N 0.000 claims description 4
- 229950011321 azaserine Drugs 0.000 claims description 4
- 230000010076 replication Effects 0.000 claims description 4
- 108020004705 Codon Proteins 0.000 claims description 3
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims description 3
- 229940009098 aspartate Drugs 0.000 claims description 3
- 230000004927 fusion Effects 0.000 claims description 3
- 238000002708 random mutagenesis Methods 0.000 claims description 3
- 108010039491 Ricin Proteins 0.000 claims description 2
- 230000006872 improvement Effects 0.000 description 35
- 102000004169 proteins and genes Human genes 0.000 description 17
- 239000000203 mixture Substances 0.000 description 13
- 230000021615 conjugation Effects 0.000 description 12
- 230000008826 genomic mutation Effects 0.000 description 10
- GVEZIHKRYBHEFX-MNOVXSKESA-N 13C-Cerulenin Natural products CC=CCC=CCCC(=O)[C@H]1O[C@@H]1C(N)=O GVEZIHKRYBHEFX-MNOVXSKESA-N 0.000 description 8
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 description 8
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 8
- GVEZIHKRYBHEFX-UHFFFAOYSA-N caerulein A Natural products CC=CCC=CCCC(=O)C1OC1C(N)=O GVEZIHKRYBHEFX-UHFFFAOYSA-N 0.000 description 8
- GVEZIHKRYBHEFX-NQQPLRFYSA-N cerulenin Chemical compound C\C=C\C\C=C\CCC(=O)[C@H]1O[C@H]1C(N)=O GVEZIHKRYBHEFX-NQQPLRFYSA-N 0.000 description 8
- 229950005984 cerulenin Drugs 0.000 description 8
- TZBJGXHYKVUXJN-UHFFFAOYSA-N genistein Natural products C1=CC(O)=CC=C1C1=COC2=CC(O)=CC(O)=C2C1=O TZBJGXHYKVUXJN-UHFFFAOYSA-N 0.000 description 8
- JFLRKDZMHNBDQS-UCQUSYKYSA-N CC[C@H]1CCC[C@@H]([C@H](C(=O)C2=C[C@H]3[C@@H]4C[C@@H](C[C@H]4C(=C[C@H]3[C@@H]2CC(=O)O1)C)O[C@H]5[C@@H]([C@@H]([C@H]([C@@H](O5)C)OC)OC)OC)C)O[C@H]6CC[C@@H]([C@H](O6)C)N(C)C.CC[C@H]1CCC[C@@H]([C@H](C(=O)C2=C[C@H]3[C@@H]4C[C@@H](C[C@H]4C=C[C@H]3C2CC(=O)O1)O[C@H]5[C@@H]([C@@H]([C@H]([C@@H](O5)C)OC)OC)OC)C)O[C@H]6CC[C@@H]([C@H](O6)C)N(C)C Chemical compound CC[C@H]1CCC[C@@H]([C@H](C(=O)C2=C[C@H]3[C@@H]4C[C@@H](C[C@H]4C(=C[C@H]3[C@@H]2CC(=O)O1)C)O[C@H]5[C@@H]([C@@H]([C@H]([C@@H](O5)C)OC)OC)OC)C)O[C@H]6CC[C@@H]([C@H](O6)C)N(C)C.CC[C@H]1CCC[C@@H]([C@H](C(=O)C2=C[C@H]3[C@@H]4C[C@@H](C[C@H]4C=C[C@H]3C2CC(=O)O1)O[C@H]5[C@@H]([C@@H]([C@H]([C@@H](O5)C)OC)OC)OC)C)O[C@H]6CC[C@@H]([C@H](O6)C)N(C)C JFLRKDZMHNBDQS-UCQUSYKYSA-N 0.000 description 6
- 108010000659 Choline oxidase Proteins 0.000 description 6
- 241000187560 Saccharopolyspora Species 0.000 description 6
- 239000005930 Spinosad Substances 0.000 description 6
- 230000003115 biocidal effect Effects 0.000 description 6
- 230000001747 exhibiting effect Effects 0.000 description 6
- 229940045109 genistein Drugs 0.000 description 6
- 235000006539 genistein Nutrition 0.000 description 6
- ZCOLJUOHXJRHDI-CMWLGVBASA-N genistein 7-O-beta-D-glucoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC(O)=C2C(=O)C(C=3C=CC(O)=CC=3)=COC2=C1 ZCOLJUOHXJRHDI-CMWLGVBASA-N 0.000 description 6
- 101150025220 sacB gene Proteins 0.000 description 6
- 229940014213 spinosad Drugs 0.000 description 6
- 229940079593 drug Drugs 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 101150080777 pheS gene Proteins 0.000 description 5
- 229940096701 plain lipid modifying drug hmg coa reductase inhibitors Drugs 0.000 description 5
- 201000001718 Roberts syndrome Diseases 0.000 description 4
- 208000012474 Roberts-SC phocomelia syndrome Diseases 0.000 description 4
- 239000003242 anti bacterial agent Substances 0.000 description 4
- 229940088710 antibiotic agent Drugs 0.000 description 4
- 238000002744 homologous recombination Methods 0.000 description 4
- 230000006801 homologous recombination Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 230000007935 neutral effect Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000005001 rutherford backscattering spectroscopy Methods 0.000 description 4
- 101100437498 Escherichia coli (strain K12) uidA gene Proteins 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 239000004472 Lysine Substances 0.000 description 3
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 3
- 238000010362 genome editing Methods 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 206010064571 Gene mutation Diseases 0.000 description 2
- 102100034343 Integrase Human genes 0.000 description 2
- 108010061833 Integrases Proteins 0.000 description 2
- LRQKBLKVPFOOQJ-YFKPBYRVSA-N L-norleucine Chemical compound CCCC[C@H]([NH3+])C([O-])=O LRQKBLKVPFOOQJ-YFKPBYRVSA-N 0.000 description 2
- 108700008625 Reporter Genes Proteins 0.000 description 2
- 102000008579 Transposases Human genes 0.000 description 2
- 108010020764 Transposases Proteins 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- XZNUGFQTQHRASN-XQENGBIVSA-N apramycin Chemical compound O([C@H]1O[C@@H]2[C@H](O)[C@@H]([C@H](O[C@H]2C[C@H]1N)O[C@@H]1[C@@H]([C@@H](O)[C@H](N)[C@@H](CO)O1)O)NC)[C@@H]1[C@@H](N)C[C@@H](N)[C@H](O)[C@H]1O XZNUGFQTQHRASN-XQENGBIVSA-N 0.000 description 2
- 229950006334 apramycin Drugs 0.000 description 2
- 238000000295 emission spectrum Methods 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 238000000695 excitation spectrum Methods 0.000 description 2
- 238000003205 genotyping method Methods 0.000 description 2
- MHWLWQUZZRMNGJ-UHFFFAOYSA-N nalidixic acid Chemical compound C1=C(C)N=C2N(CC)C=C(C(O)=O)C(=O)C2=C1 MHWLWQUZZRMNGJ-UHFFFAOYSA-N 0.000 description 2
- 229960000210 nalidixic acid Drugs 0.000 description 2
- 150000003230 pyrimidines Chemical class 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 102000053187 Glucuronidase Human genes 0.000 description 1
- 108010060309 Glucuronidase Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 101100397226 Schizosaccharomyces pombe (strain 972 / ATCC 24843) isp4 gene Proteins 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000001268 conjugating effect Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- DKVBOUDTNWVDEP-NJCHZNEYSA-N teicoplanin aglycone Chemical class N([C@H](C(N[C@@H](C1=CC(O)=CC(O)=C1C=1C(O)=CC=C2C=1)C(O)=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)OC=1C=C3C=C(C=1O)OC1=CC=C(C=C1Cl)C[C@H](C(=O)N1)NC([C@H](N)C=4C=C(O5)C(O)=CC=4)=O)C(=O)[C@@H]2NC(=O)[C@@H]3NC(=O)[C@@H]1C1=CC5=CC(O)=C1 DKVBOUDTNWVDEP-NJCHZNEYSA-N 0.000 description 1
- 230000017105 transposition Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762515934P | 2017-06-06 | 2017-06-06 | |
| US62/515,934 | 2017-06-06 | ||
| PCT/US2018/036352 WO2018226893A2 (en) | 2017-06-06 | 2018-06-06 | A high-throughput (htp) genomic engineering platform for improving saccharopolyspora spinosa |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020524493A JP2020524493A (ja) | 2020-08-20 |
| JP2020524493A5 true JP2020524493A5 (enExample) | 2021-07-26 |
| JP7350659B2 JP7350659B2 (ja) | 2023-09-26 |
Family
ID=62749236
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019567368A Active JP7350659B2 (ja) | 2017-06-06 | 2018-06-06 | Saccharopolyspora spinosaの改良のためのハイスループット(HTP)ゲノム操作プラットフォーム |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20200115705A1 (enExample) |
| EP (1) | EP3635110A2 (enExample) |
| JP (1) | JP7350659B2 (enExample) |
| KR (1) | KR102741381B1 (enExample) |
| CN (1) | CN110914425B (enExample) |
| CA (1) | CA3064619A1 (enExample) |
| WO (1) | WO2018226893A2 (enExample) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109979531B (zh) * | 2019-03-29 | 2021-08-31 | 北京市商汤科技开发有限公司 | 一种基因变异识别方法、装置和存储介质 |
| WO2020227299A1 (en) * | 2019-05-08 | 2020-11-12 | Zymergen Inc. | Downscaling parameters to design experiments and plate models for micro-organisms at small scale to improve prediction of performance at larger scale |
| CA3148478A1 (en) * | 2019-08-22 | 2021-02-25 | Karl Anton Grothe KREMLING | Methods and systems for assessing genetic variants |
| CN115516079A (zh) | 2020-04-27 | 2022-12-23 | 巴斯夫欧洲公司 | 用于红霉素发酵生产的发酵培养基和方法 |
| CN111548980B (zh) * | 2020-06-16 | 2022-09-20 | 华东理工大学 | 一种重组红霉素工程菌及其构建方法和筛选方法和应用 |
| JP7329221B2 (ja) * | 2020-08-13 | 2023-08-18 | 江南大学 | サッカロポリスポラ組成物及びその食品における使用 |
| CN111979146B (zh) * | 2020-08-13 | 2022-05-10 | 江南大学 | 糖多孢菌及其在食品中的应用 |
| WO2022051505A1 (en) * | 2020-09-03 | 2022-03-10 | Melonfrost, Inc. | Machine learning and control systems and methods for learning and steering evolutionary dynamics |
| WO2022082362A1 (zh) * | 2020-10-19 | 2022-04-28 | 陈振暐 | 代谢酪氨酸的非病原性细菌基因表现系统及转化株、其用于制备降低尿毒素的组合物的用途以及利用其代谢酪氨酸的方法 |
| WO2022235417A1 (en) * | 2021-05-01 | 2022-11-10 | John Mcdevitt | System and method for improved carbon sequestration by means of improved genetic modification of algae |
| CN113249268B (zh) * | 2021-06-25 | 2023-04-07 | 江南大学 | 一株降低生物胺的玫瑰糖多孢菌及其应用 |
| US11530406B1 (en) | 2021-08-30 | 2022-12-20 | Sachi Bioworks Inc. | System and method for producing a therapeutic oligomer |
| US12037621B2 (en) | 2021-09-15 | 2024-07-16 | Archer-Daniels-Midland Company | Threonine production strain having attenuated expression of the yafV gene |
| CN113897324B (zh) * | 2021-10-13 | 2023-07-28 | 云南师范大学 | 一种用作抗锰剂的JcVIPP1重组大肠杆菌及其构建方法 |
| CN116121140A (zh) * | 2023-01-10 | 2023-05-16 | 黄河三角洲京博化工研究院有限公司 | 一株耐高浓度sam的刺糖多胞菌及其应用 |
| KR102838147B1 (ko) * | 2023-03-17 | 2025-07-28 | 씨제이제일제당 주식회사 | 5'-utr 변이 서열 및 이의 용도 |
| CN117286181B (zh) * | 2023-11-24 | 2024-03-01 | 广东省农业科学院作物研究所 | 一种CRISPR/Cas9介导的四倍体广藿香高效靶向诱变的基因编辑系统 |
Family Cites Families (99)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4206206A (en) | 1977-03-24 | 1980-06-03 | Kowa Company, Ltd. | Antibiotics of the KA-6606 series and pharmaceutical compositions thereof |
| US4328307A (en) | 1977-03-24 | 1982-05-04 | Kowa Company, Ltd. | Novel antibiotics, process for preparation thereof and biologically pure culture for use therein |
| US4251511A (en) | 1979-10-02 | 1981-02-17 | The Upjohn Company | Antibiotic and fermentation process of preparing |
| US4293651A (en) | 1979-10-02 | 1981-10-06 | The Upjohn Company | Process for producing antibiotic using saccharopolyspora |
| EP0044477B1 (en) | 1980-07-15 | 1984-02-08 | Kowa Company, Ltd. | Process for production of antibiotics, and novel antibiotics produced thereby |
| US4435504A (en) | 1982-07-15 | 1984-03-06 | Syva Company | Immunochromatographic assay with support having bound "MIP" and second enzyme |
| GB8406752D0 (en) | 1984-03-15 | 1984-04-18 | Unilever Plc | Chemical and clinical tests |
| DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| CA1303983C (en) | 1987-03-27 | 1992-06-23 | Robert W. Rosenstein | Solid phase assay |
| US4855240A (en) | 1987-05-13 | 1989-08-08 | Becton Dickinson And Company | Solid phase assay employing capillary flow |
| US5187088A (en) | 1988-08-26 | 1993-02-16 | Takeda Chemical Industries, Ltd. | Choline oxidase and method for producing the same |
| US5171740A (en) | 1988-10-21 | 1992-12-15 | Abbott Laboratories | Coumamidine compounds |
| US5362634A (en) | 1989-10-30 | 1994-11-08 | Dowelanco | Process for producing A83543 compounds |
| MA21697A1 (fr) | 1988-12-19 | 1990-07-01 | Dow Agrosciences Llc | Composes de macrolides. |
| JP2787458B2 (ja) | 1989-01-20 | 1998-08-20 | 旭化成工業株式会社 | 抗生物質l53―18aおよびその製造法 |
| US5198360A (en) | 1990-01-19 | 1993-03-30 | Eli Lilly And Company | Dna sequence conferring a plaque inhibition phenotype |
| US5153128A (en) | 1990-03-16 | 1992-10-06 | Suntory Limited | Heat-resistant β-galactosyltransferase, its production process and its use |
| US5234828A (en) | 1990-03-16 | 1993-08-10 | Suntory Limited | Process for producing novel heat-resistant β-galactosyltransferase |
| US5124258A (en) | 1990-09-12 | 1992-06-23 | Merck & Co., Inc. | Fermentation process for the preparation of ivermectin aglycone |
| US5824513A (en) | 1991-01-17 | 1998-10-20 | Abbott Laboratories | Recombinant DNA method for producing erythromycin analogs |
| US6060234A (en) | 1991-01-17 | 2000-05-09 | Abbott Laboratories | Polyketide derivatives and recombinant methods for making same |
| US6060296A (en) | 1991-07-03 | 2000-05-09 | The Salk Institute For Biological Studies | Protein kinases |
| WO1993004169A1 (en) | 1991-08-20 | 1993-03-04 | Genpharm International, Inc. | Gene targeting in animal cells using isogenic dna constructs |
| US5202242A (en) | 1991-11-08 | 1993-04-13 | Dowelanco | A83543 compounds and processes for production thereof |
| US5591606A (en) | 1992-11-06 | 1997-01-07 | Dowelanco | Process for the production of A83543 compounds with Saccharopolyspora spinosa |
| BR9406587A (pt) | 1993-03-12 | 1996-01-02 | Dowelanco | Novos compostos a83543 e processo para a produção dos mesmos |
| US6500960B1 (en) | 1995-07-06 | 2002-12-31 | Stanford University (Board Of Trustees Of The Leland Stanford Junior University) | Method to produce novel polyketides |
| US6043064A (en) | 1993-10-22 | 2000-03-28 | Bristol-Myers Squibb Company | Enzymatic hydroxylation process for the preparation of HMG-CoA reductase inhibitors and intermediates thereof |
| US5837458A (en) | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US6117679A (en) * | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US6090592A (en) | 1994-08-03 | 2000-07-18 | Mosaic Technologies, Inc. | Method for performing amplification of nucleic acid on supports |
| US5801032A (en) | 1995-08-03 | 1998-09-01 | Abbott Laboratories | Vectors and process for producing high purity 6,12-dideoxyerythromycin A by fermentation |
| US5554519A (en) | 1995-08-07 | 1996-09-10 | Fermalogic, Inc. | Process of preparing genistein |
| US6960453B1 (en) | 1996-07-05 | 2005-11-01 | Biotica Technology Limited | Hybrid polyketide synthases combining heterologous loading and extender modules |
| US6271255B1 (en) | 1996-07-05 | 2001-08-07 | Biotica Technology Limited | Erythromycins and process for their preparation |
| US5663067A (en) | 1996-07-11 | 1997-09-02 | New England Biolabs, Inc. | Method for cloning and producing the SapI restriction endonuclease in E. coli |
| US6326204B1 (en) * | 1997-01-17 | 2001-12-04 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
| AU743305C (en) * | 1997-01-17 | 2006-03-30 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
| WO1998044151A1 (en) | 1997-04-01 | 1998-10-08 | Glaxo Group Limited | Method of nucleic acid amplification |
| US5908764A (en) | 1997-05-22 | 1999-06-01 | Solidago Ag | Methods and compositions for increasing production of erythromycin |
| JPH1180185A (ja) | 1997-09-05 | 1999-03-26 | Res Dev Corp Of Japan | オリゴヌクレオチドの化学合成法 |
| US6420177B1 (en) | 1997-09-16 | 2002-07-16 | Fermalogic Inc. | Method for strain improvement of the erythromycin-producing bacterium |
| EP0974657A1 (en) | 1998-06-26 | 2000-01-26 | Rijksuniversiteit te Leiden | Reducing branching and enhancing fragmentation in culturing filamentous microorganisms |
| GB9814006D0 (en) | 1998-06-29 | 1998-08-26 | Biotica Tech Ltd | Polyketides and their synthesis |
| AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
| WO2000026349A2 (en) | 1998-10-29 | 2000-05-11 | Kosan Biosciences, Inc. | Recombinant oleandolide polyketide synthase |
| US6780620B1 (en) | 1998-12-23 | 2004-08-24 | Bristol-Myers Squibb Company | Microbial transformation method for the preparation of an epothilone |
| CA2411293A1 (en) | 1999-01-28 | 2000-07-28 | Pfizer Products Inc. | Novel azalides and methods of making same |
| DE60025799T2 (de) | 1999-04-05 | 2006-10-19 | Sumitomo Chemical Co., Ltd. | Verfahren zur Herstellung von optisch aktiven Aminosäuren |
| US6300070B1 (en) | 1999-06-04 | 2001-10-09 | Mosaic Technologies, Inc. | Solid phase methods for amplifying multiple nucleic acids |
| US6365399B1 (en) | 1999-08-09 | 2002-04-02 | Sumitomo Chemical Company, Limited | Process for producing carboxylic acid isomer using Nocardia diaphanozonaria or Saccharopolyspora hirsuta |
| US6524841B1 (en) | 1999-10-08 | 2003-02-25 | Kosan Biosciences, Inc. | Recombinant megalomicin biosynthetic genes and uses thereof |
| DE60026563D1 (de) | 1999-10-25 | 2006-05-04 | Kosan Biosciences Inc | Herstellung von polyketiden |
| US6861513B2 (en) | 2000-01-12 | 2005-03-01 | Schering Corporation | Everninomicin biosynthetic genes |
| WO2001075116A2 (en) | 2000-04-04 | 2001-10-11 | Schering Corporation | ISOLATED NUCLEIC ACIDS FROM MICROMONOSPORA ROSARIA PLASMID pMR2 AND VECTORS MADE THEREFROM |
| JP2005529579A (ja) | 2000-05-02 | 2005-10-06 | コーサン バイオサイエンシーズ, インコーポレイテッド | ポリケチドの生合成のための過剰産生宿主 |
| WO2001087936A2 (en) | 2000-05-17 | 2001-11-22 | Schering Corporation | Isolation of micromonospora carbonacea var africana pmlp1 integrase and use of integrating function for site-specific integration into micromonospora halophitica and micromonospora carbonacea chromosome |
| WO2002029032A2 (en) * | 2000-09-30 | 2002-04-11 | Diversa Corporation | Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating |
| US6616953B2 (en) | 2001-01-02 | 2003-09-09 | Abbott Laboratories | Concentrated spent fermentation beer or saccharopolyspora erythraea activated by an enzyme mixture as a nutritional feed supplement |
| US7630836B2 (en) | 2001-05-30 | 2009-12-08 | The Kitasato Institute | Polynucleotides |
| US20030131370A1 (en) | 2001-12-14 | 2003-07-10 | Pfizer Inc. | Disruption of the glutathione S-transferase-Omega-1 gene |
| US20030157076A1 (en) | 2002-02-08 | 2003-08-21 | Pfizer Inc. | Disruption of the Akt2 gene |
| DK1492404T3 (da) | 2002-02-19 | 2012-04-23 | Dow Agrosciences Llc | Nye spinosyndannende polyketidsyntaser |
| EP1361270A3 (en) | 2002-03-30 | 2004-01-02 | Pfizer Products Inc. | Disruption of the REDK gene |
| US7459294B2 (en) | 2003-08-08 | 2008-12-02 | Kosan Biosciences Incorporated | Method of producing a compound by fermentation |
| WO2005021772A1 (en) | 2003-08-29 | 2005-03-10 | Degussa Ag | Process for the preparation of l-lysine |
| CN101223281A (zh) * | 2005-07-18 | 2008-07-16 | 巴斯福股份公司 | 芽孢杆菌MetI基因提高微生物中甲硫氨酸产量的用途 |
| WO2008020827A2 (en) * | 2005-08-01 | 2008-02-21 | Biogen Idec Ma Inc. | Altered polypeptides, immunoconjugates thereof, and methods related thereto |
| MX2008015213A (es) | 2006-05-30 | 2008-12-09 | Dow Global Technologies Inc | Metodo de optimizacion de codon. |
| US8841092B2 (en) | 2006-08-30 | 2014-09-23 | Wisconsin Alumni Research Foundation | Reversible natural product glycosyltransferase-catalyzed reactions, compounds and related methods |
| EP2171087B1 (en) * | 2007-06-15 | 2015-02-25 | E. I. Du Pont de Nemours and Company | Polynucleotides and methods for making plants resistant to fungal pathogens |
| US9267132B2 (en) * | 2007-10-08 | 2016-02-23 | Synthetic Genomics, Inc. | Methods for cloning and manipulating genomes |
| AU2009266989B2 (en) * | 2008-07-03 | 2013-05-02 | Pfenex, Inc. | High throughput screening method and use thereof to identify a production platform for a multifunctional binding protein |
| US8808986B2 (en) | 2008-08-27 | 2014-08-19 | Gen9, Inc. | Methods and devices for high fidelity polynucleotide synthesis |
| WO2010029585A1 (en) | 2008-09-10 | 2010-03-18 | Bormioli Rocco & Figlio S.P.A. | Security capsule with breakable reservoir and cutter |
| US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
| US8783382B2 (en) | 2009-01-15 | 2014-07-22 | Schlumberger Technology Corporation | Directional drilling control devices and methods |
| WO2010094772A1 (en) | 2009-02-20 | 2010-08-26 | Febit Holding Gmbh | Synthesis of sequence-verified nucleic acids |
| US8426189B2 (en) | 2009-04-29 | 2013-04-23 | Fermalogic, Inc. | Soybean-based fermentation media, methods of making and use |
| US8574835B2 (en) | 2009-05-29 | 2013-11-05 | Life Technologies Corporation | Scaffolded nucleic acid polymer particles and methods of making and using |
| EP2395087A1 (en) | 2010-06-11 | 2011-12-14 | Icon Genetics GmbH | System and method of modular cloning |
| AU2015224510B2 (en) * | 2010-08-30 | 2017-11-16 | Dow Agrosciences Llc | Activation tagging platform for maize, and resultant tagged population and plants |
| US9334514B2 (en) | 2010-10-29 | 2016-05-10 | The Regents Of The University Of California | Hybrid polyketide synthases |
| FR2968313B1 (fr) | 2010-12-03 | 2014-10-10 | Lesaffre & Cie | Procede de preparation d'une levure industrielle, levure industrielle et application a la production d'ethanol a partir d'au moins un pentose |
| US20150368639A1 (en) * | 2011-04-14 | 2015-12-24 | Ryan T. Gill | Compositions, methods and uses for multiplex protein sequence activity relationship mapping |
| US8741603B2 (en) | 2011-05-03 | 2014-06-03 | Agrigenetics Inc. | Enhancing spinosyn production with oxygen binding proteins |
| DK2520653T3 (en) * | 2011-05-03 | 2017-07-03 | Dow Agrosciences Llc | INTEGRATION OF GENES IN THE CHROMOSOME OF SACCHAROPOLYSPORA SPINOSA |
| US9631195B2 (en) * | 2011-12-28 | 2017-04-25 | Dow Agrosciences Llc | Identification and characterization of the spinactin biosysnthesis gene cluster from spinosyn producing saccharopolyspora spinosa |
| EP2677034A1 (en) | 2012-06-18 | 2013-12-25 | LEK Pharmaceuticals d.d. | Genome sequence based targeted cloning of DNA fragments |
| GB201312318D0 (en) | 2013-07-09 | 2013-08-21 | Isomerase Therapeutics Ltd | Novel methods and compounds |
| CN105087507B (zh) | 2014-05-14 | 2019-01-25 | 中国科学院上海生命科学研究院 | 一种整合酶及其在改造刺糖多孢菌中的应用 |
| SG11201703472YA (en) | 2014-11-05 | 2017-05-30 | Illumina Inc | Transposase compositions for reduction of insertion bias |
| GB201421859D0 (en) * | 2014-12-09 | 2015-01-21 | Bactevo Ltd | Method for screening for natural products |
| KR102356072B1 (ko) | 2015-09-10 | 2022-01-27 | 에스케이하이닉스 주식회사 | 메모리 시스템 및 그 동작 방법 |
| JP6821598B2 (ja) * | 2015-12-07 | 2021-01-27 | ザイマージェン インコーポレイテッド | Corynebacterium glutamicum由来のプロモーター |
| US11151497B2 (en) * | 2016-04-27 | 2021-10-19 | Zymergen Inc. | Microbial strain design system and methods for improved large-scale production of engineered nucleotide sequences |
| EP3858996B1 (en) * | 2015-12-07 | 2022-08-03 | Zymergen Inc. | Microbial strain improvement by a htp genomic engineering platform |
| US9988624B2 (en) * | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
-
2018
- 2018-06-06 JP JP2019567368A patent/JP7350659B2/ja active Active
- 2018-06-06 KR KR1020197038679A patent/KR102741381B1/ko active Active
- 2018-06-06 CN CN201880047656.5A patent/CN110914425B/zh active Active
- 2018-06-06 CA CA3064619A patent/CA3064619A1/en active Pending
- 2018-06-06 US US16/620,203 patent/US20200115705A1/en not_active Abandoned
- 2018-06-06 WO PCT/US2018/036352 patent/WO2018226893A2/en not_active Ceased
- 2018-06-06 EP EP18734409.8A patent/EP3635110A2/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2020524493A5 (enExample) | ||
| CN104017821B (zh) | 定向编辑颖壳颜色决定基因OsCHI创制褐壳水稻材料的方法 | |
| US7244609B2 (en) | Synthetic genes and bacterial plasmids devoid of CpG | |
| US11732251B2 (en) | Anti-CRISPR polynucleotides and polypeptides and methods of use | |
| JP2021087454A (ja) | Crisprにより可能にされる多重ゲノムエンジニアリング | |
| Wang et al. | Multiplexed promoter and gene editing in wheat using a virus‐based guide RNA delivery system | |
| CN108611364A (zh) | 一种非转基因crispr突变体的制备方法 | |
| JP2010539994A5 (enExample) | ||
| JP2019022516A (ja) | 複数の宿主−共生生物アソシエーションをスクリーニングすることによる共生体の選択 | |
| US20250250580A1 (en) | Polyploid hybrid maize breeding | |
| Florea et al. | Chromosome-end knockoff strategy to reshape alkaloid profiles of a fungal endophyte | |
| Kawaguchi et al. | Lotus burttii takes a position of the third corner in the Lotus molecular genetics triangle | |
| Zhou et al. | Regeneration and Agrobacterium-mediated genetic transformation of twelve Eucalyptus species | |
| Wang et al. | Long noncoding RNAs underlie multiple domestication traits and leafhopper resistance in soybean | |
| CN107227303B (zh) | 一种OsGA3ox1基因在水稻雄性不育株系创制中的应用 | |
| CN113493803B (zh) | 紫花苜蓿CRISPR/Cas9基因组编辑系统及其应用 | |
| Lee et al. | Their C-termini divide Brassica rapa FT-like proteins into FD-interacting and FD-independent proteins that have different effects on the floral transition | |
| Baum | Identifying the genetic causes of phenotypic evolution: a review of experimental strategies | |
| JP2025532306A (ja) | 大規模直交型CRE媒介組換えのための新たなLoxPsym部位 | |
| CN108342408A (zh) | 一种精确控制基因重排的基因元件及其重组质粒和应用 | |
| CN113046361A (zh) | 基于NtFER基因的改造在提高植物青枯病抗性中的应用 | |
| US20250270573A1 (en) | Systems and methods for genome-scale targeting of functional redundancy in plants | |
| AU2021225232B2 (en) | Novel endophytes (4) | |
| Guedes et al. | Idioblasts accumulating anticancer alkaloids in Catharanthus roseus leaves are a unique cell type | |
| Kölzsch | Approaches to generate herbicide resistant Taraxacum koksaghyz by directed and undirected mutagenesis of the acetohydroxyacid synthase |