SG11201703472YA - Transposase compositions for reduction of insertion bias - Google Patents

Transposase compositions for reduction of insertion bias

Info

Publication number
SG11201703472YA
SG11201703472YA SG11201703472YA SG11201703472YA SG11201703472YA SG 11201703472Y A SG11201703472Y A SG 11201703472YA SG 11201703472Y A SG11201703472Y A SG 11201703472YA SG 11201703472Y A SG11201703472Y A SG 11201703472YA SG 11201703472Y A SG11201703472Y A SG 11201703472YA
Authority
SG
Singapore
Prior art keywords
reduction
insertion bias
transposase
compositions
transposase compositions
Prior art date
Application number
SG11201703472YA
Inventor
Christian Gloeckner
Amirali Kia
Molly He
Trina Faye Osothprarop
Frank J Steemers
Kevin L Gunderson
Sasan Amini
Jerome Jendrisak
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of SG11201703472YA publication Critical patent/SG11201703472YA/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07Nucleotidyltransferases (2.7.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/185Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Infusion, Injection, And Reservoir Apparatuses (AREA)
  • Fuel-Injection Apparatus (AREA)
  • Mechanical Coupling Of Light Guides (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Non-Portable Lighting Devices Or Systems Thereof (AREA)
  • Endoscopes (AREA)
  • Fittings On The Vehicle Exterior For Carrying Loads, And Devices For Holding Or Mounting Articles (AREA)
SG11201703472YA 2014-11-05 2015-11-05 Transposase compositions for reduction of insertion bias SG11201703472YA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462075713P 2014-11-05 2014-11-05
US201562242935P 2015-10-16 2015-10-16
PCT/US2015/059194 WO2016073690A1 (en) 2014-11-05 2015-11-05 Transposase compositions for reduction of insertion bias

Publications (1)

Publication Number Publication Date
SG11201703472YA true SG11201703472YA (en) 2017-05-30

Family

ID=54697644

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201703472YA SG11201703472YA (en) 2014-11-05 2015-11-05 Transposase compositions for reduction of insertion bias

Country Status (10)

Country Link
US (2) US10815478B2 (en)
EP (3) EP4239067A3 (en)
CN (1) CN107532164A (en)
AU (2) AU2015343034B2 (en)
CA (1) CA2965995A1 (en)
DK (2) DK3215618T3 (en)
ES (1) ES2947757T3 (en)
FI (1) FI3628732T3 (en)
SG (1) SG11201703472YA (en)
WO (1) WO2016073690A1 (en)

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* Cited by examiner, † Cited by third party
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AU2015343034B2 (en) * 2014-11-05 2021-12-16 Illumina, Inc. Transposase compositions for reduction of insertion bias
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
CA3007635A1 (en) 2015-12-07 2017-06-15 Zymergen Inc. Promoters from corynebacterium glutamicum
JP6605042B2 (en) 2015-12-07 2019-11-13 ザイマージェン インコーポレイテッド Improvement of microbial strain by HTP genome manipulation platform
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
US10544390B2 (en) 2016-06-30 2020-01-28 Zymergen Inc. Methods for generating a bacterial hemoglobin library and uses thereof
EP3478845A4 (en) 2016-06-30 2019-07-31 Zymergen, Inc. Methods for generating a glucose permease library and uses thereof
CA3033506A1 (en) * 2016-08-10 2018-02-15 President And Fellows Of Harvard College Methods of de novo assembly of barcoded genomic dna fragments
CA3048863A1 (en) * 2016-12-29 2018-07-05 Illumina, Inc. Analysis system for orthogonal access to and tagging of biomolecules in cellular compartments
EP3625351A1 (en) 2017-05-19 2020-03-25 Zymergen Inc. Genomic engineering of biosynthetic pathways leading to increased nadph
KR20200015606A (en) 2017-06-06 2020-02-12 지머젠 인코포레이티드 High Throughput (HTP) Genome Engineering Platform for Improving Saccharopolis Fora Spinosa
US20200370058A1 (en) 2017-06-06 2020-11-26 Zymergen Inc. A htp genomic engineering platform for improving escherichia coli
KR20200026878A (en) 2017-06-06 2020-03-11 지머젠 인코포레이티드 HTP Genome Engineering Platform to Improve Fungal Strains
WO2018226810A1 (en) 2017-06-06 2018-12-13 Zymergen Inc. High throughput transposon mutagenesis
WO2019183183A1 (en) 2018-03-20 2019-09-26 Zymergen Inc. A htp platform for the genetic engineering of chinese hamster ovary cells
US11773441B2 (en) 2018-05-03 2023-10-03 Becton, Dickinson And Company High throughput multiomics sample analysis
US20220195624A1 (en) 2019-01-29 2022-06-23 Mgi Tech Co., Ltd. High coverage stlfr
CN113226519A (en) * 2019-07-12 2021-08-06 Illumina剑桥有限公司 Preparation of nucleic acid libraries using electrophoresis
US11377655B2 (en) 2019-07-16 2022-07-05 Pacific Biosciences Of California, Inc. Synthetic nucleic acids having non-natural structures
EP4103580A4 (en) 2020-02-13 2024-03-06 Zymergen Inc. Metagenomic library and natural product discovery platform
WO2024003332A1 (en) * 2022-06-30 2024-01-04 F. Hoffmann-La Roche Ag Controlling for tagmentation sequencing library insert size using archaeal histone-like proteins

Family Cites Families (16)

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Publication number Priority date Publication date Assignee Title
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
FI103809B1 (en) * 1997-07-14 1999-09-30 Finnzymes Oy In vitro method for producing templates for DNA sequencing
CN1181852C (en) * 2002-05-21 2004-12-29 崔行善 Medicine for treating bladder cancer
FR2900935A1 (en) * 2006-05-15 2007-11-16 Centre Nat Rech Scient RECOMBINANT HYPERACTIVE TRANSPOSITION SYSTEMS DERIVED FROM TRANSPOSON MOS-1
PT2963709T (en) 2008-10-24 2017-08-21 Epicentre Tech Corp Transposon end compositions and methods for modifying nucleic acids
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
CA2821299C (en) * 2010-11-05 2019-02-12 Frank J. Steemers Linking sequence reads using paired code tags
US9365897B2 (en) 2011-02-08 2016-06-14 Illumina, Inc. Selective enrichment of nucleic acids
US9005935B2 (en) 2011-05-23 2015-04-14 Agilent Technologies, Inc. Methods and compositions for DNA fragmentation and tagging by transposases
US20130143774A1 (en) 2011-12-05 2013-06-06 The Regents Of The University Of California Methods and compositions for generating polynucleic acid fragments
US9644199B2 (en) 2012-10-01 2017-05-09 Agilent Technologies, Inc. Immobilized transposase complexes for DNA fragmentation and tagging
US9683230B2 (en) * 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
WO2014205296A1 (en) * 2013-06-21 2014-12-24 The Broad Institute, Inc. Methods for shearing and tagging dna for chromatin immunoprecipitation and sequencing
WO2015089339A2 (en) * 2013-12-11 2015-06-18 Changping Shi Compositions, methods and kits for dna fragmentation and tagmentation
CN106507677B (en) * 2014-04-15 2021-03-12 伊鲁米那股份有限公司 Modified transposases for improved insert sequence bias and increased DNA import tolerance
AU2015343034B2 (en) * 2014-11-05 2021-12-16 Illumina, Inc. Transposase compositions for reduction of insertion bias

Also Published As

Publication number Publication date
DK3215618T3 (en) 2019-10-14
EP4239067A2 (en) 2023-09-06
FI3628732T3 (en) 2023-05-31
AU2022201248A1 (en) 2022-03-17
CA2965995A1 (en) 2016-05-12
US10815478B2 (en) 2020-10-27
EP3215618B1 (en) 2019-07-24
US20180016571A1 (en) 2018-01-18
EP3215618A1 (en) 2017-09-13
US20210047635A1 (en) 2021-02-18
EP3628732B1 (en) 2023-05-03
AU2015343034A1 (en) 2017-05-18
AU2015343034A9 (en) 2019-08-01
ES2947757T3 (en) 2023-08-18
AU2015343034B2 (en) 2021-12-16
WO2016073690A1 (en) 2016-05-12
CN107532164A (en) 2018-01-02
DK3628732T3 (en) 2023-05-30
EP4239067A3 (en) 2023-10-25
WO2016073690A9 (en) 2017-09-14
EP3628732A1 (en) 2020-04-01

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