JP2020519304A5 - - Google Patents
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- JP2020519304A5 JP2020519304A5 JP2020500937A JP2020500937A JP2020519304A5 JP 2020519304 A5 JP2020519304 A5 JP 2020519304A5 JP 2020500937 A JP2020500937 A JP 2020500937A JP 2020500937 A JP2020500937 A JP 2020500937A JP 2020519304 A5 JP2020519304 A5 JP 2020519304A5
- Authority
- JP
- Japan
- Prior art keywords
- exonuclease
- nucleic acid
- acid molecule
- target nucleic
- rect
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710177676.1A CN106995813B (zh) | 2017-03-23 | 2017-03-23 | 基因组大片段直接克隆和dna多分子组装新技术 |
| CN201710177676.1 | 2017-03-23 | ||
| PCT/CN2017/000483 WO2018170614A1 (zh) | 2017-03-23 | 2017-08-02 | 基因组大片段直接克隆和dna多分子组装新技术 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020519304A JP2020519304A (ja) | 2020-07-02 |
| JP2020519304A5 true JP2020519304A5 (https=) | 2021-09-24 |
| JP7106625B2 JP7106625B2 (ja) | 2022-07-26 |
Family
ID=59431502
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020500937A Expired - Fee Related JP7106625B2 (ja) | 2017-03-23 | 2017-08-02 | ゲノム大断片のダイレクトクローニングおよびｄｎａマルチ分子構築の新手法 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20200010867A1 (https=) |
| EP (1) | EP3604524B1 (https=) |
| JP (1) | JP7106625B2 (https=) |
| KR (1) | KR102488128B1 (https=) |
| CN (1) | CN106995813B (https=) |
| CA (1) | CA3060585A1 (https=) |
| DK (1) | DK3604524T3 (https=) |
| ES (1) | ES2902210T3 (https=) |
| WO (1) | WO2018170614A1 (https=) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106995813B (zh) * | 2017-03-23 | 2020-06-16 | 山东大学 | 基因组大片段直接克隆和dna多分子组装新技术 |
| EP3728589A4 (en) * | 2017-12-22 | 2021-11-03 | G+Flas Life Sciences | CHEMICAL GENOMIC ENGINEERING MOLECULES AND PROCESSES |
| CN108676847A (zh) * | 2017-12-30 | 2018-10-19 | 深圳人体密码基因科技有限公司 | 大片段基因组的捕获探针的制备方法及试剂盒 |
| CN111088275B (zh) * | 2018-10-23 | 2023-06-27 | 黄菁 | Dna大片段的克隆方法 |
| KR20210137009A (ko) * | 2019-03-08 | 2021-11-17 | 지머젠 인코포레이티드 | 미생물에서 풀링 게놈 편집 |
| CN110846239B (zh) * | 2019-11-29 | 2022-08-23 | 南京工业大学 | 具有高同源重组效率的重组解脂耶氏酵母菌及其构建方法和应用 |
| CN112852849B (zh) * | 2019-12-31 | 2023-03-14 | 湖北伯远合成生物科技有限公司 | 一种用于大片段dna无缝组装的系统及组装方法 |
| JP2023513314A (ja) * | 2020-02-13 | 2023-03-30 | ザイマージェン インコーポレイテッド | メタゲノムライブラリーおよび天然物発見プラットフォーム |
| CN113355339B (zh) * | 2020-03-05 | 2023-01-24 | 山东大学 | 一种大型基因簇的无痕定点改造方法及其应用 |
| CN111440753A (zh) * | 2020-03-16 | 2020-07-24 | 扬州大学 | 禽致病性大肠杆菌clbH基因缺失株及其构建方法和应用 |
| CN116135982A (zh) * | 2021-11-16 | 2023-05-19 | 北京光华生明生物科技有限公司 | 用于基因片段连接的应用方法和试剂盒 |
| WO2023178544A1 (zh) * | 2022-03-23 | 2023-09-28 | 深圳华大生命科学研究院 | 表达元件组合、表达载体、宿主、应用及组装方法 |
| CN115960733B (zh) * | 2022-09-19 | 2023-11-24 | 苏州泓迅生物科技股份有限公司 | 一种用于大片段dna组装的基因工程酵母菌、构建方法及其应用 |
| CN115948445A (zh) * | 2022-12-12 | 2023-04-11 | 河南科技大学 | 谷氨酸棒杆菌CRISPR-Cpf1基因编辑系统、构建方法及应用 |
| CN116355931A (zh) * | 2023-05-29 | 2023-06-30 | 北京因诺惟康医药科技有限公司 | 一种快速分子克隆方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL120338A0 (en) * | 1997-02-27 | 1997-06-10 | Gesher Israel Advanced Biotecs | Single step DNA fragments assembly |
| WO2002062988A2 (en) | 2001-02-09 | 2002-08-15 | Gene Bridges Gmbh | Recombination method |
| EP2239327B1 (en) * | 2005-08-11 | 2015-02-25 | Synthetic Genomics, Inc. | Method for in vitro recombination |
| MY143596A (en) * | 2005-08-11 | 2011-06-15 | Synthetic Genomics Inc | In vitro recombination method |
| GB201009732D0 (en) | 2010-06-10 | 2010-07-21 | Gene Bridges Gmbh | Direct cloning |
| CN102634534A (zh) * | 2012-03-30 | 2012-08-15 | 深圳市中联生物科技开发有限公司 | 基于同源重组的核酸分子克隆方法及相关试剂盒 |
| EP3778891A1 (en) * | 2014-08-27 | 2021-02-17 | New England Biolabs, Inc. | Synthon formation |
| CN106995813B (zh) * | 2017-03-23 | 2020-06-16 | 山东大学 | 基因组大片段直接克隆和dna多分子组装新技术 |
-
2017
- 2017-03-23 CN CN201710177676.1A patent/CN106995813B/zh active Active
- 2017-08-02 WO PCT/CN2017/000483 patent/WO2018170614A1/zh not_active Ceased
- 2017-08-02 CA CA3060585A patent/CA3060585A1/en active Pending
- 2017-08-02 JP JP2020500937A patent/JP7106625B2/ja not_active Expired - Fee Related
- 2017-08-02 DK DK17901979.9T patent/DK3604524T3/da active
- 2017-08-02 KR KR1020197031095A patent/KR102488128B1/ko active Active
- 2017-08-02 ES ES17901979T patent/ES2902210T3/es active Active
- 2017-08-02 EP EP17901979.9A patent/EP3604524B1/en active Active
-
2019
- 2019-09-22 US US16/578,385 patent/US20200010867A1/en not_active Abandoned
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