CN112852849B - 一种用于大片段dna无缝组装的系统及组装方法 - Google Patents

Abstract

本发明属于分子生物学技术领域，具体涉及一种用于大片段DNA无缝组装的系统及组装方法，包括：S1、构建载体系统；S2、将大片段DNA分成多个目标片段，并将目标片段按顺序交替克隆到供给载体D1、D2上；S3、用EN1同时酶切接受载体A和酶切供给载体D1‑seg1，通过组装反应得到pBWRA‑seg1，然后采用EN2酶切pBWRA‑seg1和供给载体D2‑seg2，通过组装反应得到pBWRA‑seg1‑seg2；S4、重复步骤S3，根据确定的组装顺序将目标片段分步组装连接到接受载体A上，形成最终的载体。本发明的核酸内切酶在转基因载体构建、合成生物学、代谢通路研究等方面具有良好应用前景。

Description

一种用于大片段DNA无缝组装的系统及组装方法

技术领域

本发明涉及分子生物学技术领域，具体涉及一种用于大片段DNA无缝组装的系统及组装方法。

背景技术

DNA组装技术是合成生物学实现各种目标的重要环节及关键步骤。短DNA片段可以很容易通过PCR介导的合成方法获得，但是克隆大于10kb的DNA片段仍然面临较大挑战，首先大片段高保真扩增的能力有限，PCR介导的合成方法错误率比较高，尤其对于合成大于10kb以上的DNA，而且大片段的突变率在PCR扩增过程中也会随之增加，为了得到正确的克隆，需要进行多次克隆和测序的重复工作，甚至需要进行回复突变等操作，导致成本大幅增加，且需要花费大量的时间和精力。因此，迫切需要简单、高效和快捷的基因组DNA组装技术。随着合成生物学技术的进步，尤其是IIs型限制性内切酶的使用让更大规模的组装得以实现。研究人员先后开发出了具有代表性的Golden Gate和Gibson Assembly等由小片段组装而成大片段的体外组装技术。

Golden Gate利用IIs型限制性内切酶切出在其识别序列的外侧进行切割，产生所需的黏性末端，一次进行多个片段的无缝连接，但是现有的IIs型限制性内切酶识别的最长碱基数为7bp，如AarI和SapI，平均4⁷/2＝8192bp就会至少存在一个酶切位点，难以支持更大片段的连接，一旦遇到超过10kb的超大片段一般就不能用Golden Gate构建。本实验室前期基于Golden Gate原理建立的一种多片段DNA分子组装方法及应用(BioWalk 1.0系统)、专利号：ZL201310094572.6就遇到这样的问题，若需要继续组装就必须通过密码子优化掉该酶切位点，但随着组装的载体越来越大，例如一些启动子含有较多操作的酶切位点，就很难再继续组装下去。

为克服内切酶产生黏性末端基数不足的缺陷，Gibson等放弃限制性内切酶，采用5’核酸外切酶，联合DNA聚合酶以及DNA连接酶，建立了的不受酶切位点限制的重组技术，但是同时重组超过5个片段就会导致重组效率显著降低，而且重组好的大片段载体若要继续修改就会由于没有合适的单一酶切位点切割而无法继续组装。

随着功能基因组学等研究的深入，数十个基因相互协调的研究已经越来越广泛，而几个基因进行转化已不能胜任。因此，将数十个基因构建在同一个载体上并且进行一次性转化的技术将非常必要。目前虽然有一些大片段的多基因组装系统，但是操作相对较复杂，且不能实现无缝连续组装。开发一种适用于多个大片段的无缝DNA组装方法就显得尤为重要。

发明内容

为了解决现有技术存在的问题，本发明提供了一种用于大片段DNA无缝组装的系统及组装方法，通过人工设计的RNA介导的LbCas12a核酸内切酶能够支持更大片段的连接，适用于大片段DNA分段无缝组装成一个更大的完整片段，在含有多个基因的转基因载体构建、合成生物学、代谢通路研究等方面具有良好的应用前景。

本发明的目的一在于提供：一种用于大片段DNA无缝组装的系统，包括由接受载体A(pBWRA)、供给载体D1(pBWRD1)和供给载体D2(pBWRD2)组成的系统；

所述接受载体A的多克隆位点中含有一组核酸内切酶识别靶位点EN1，且按照：“载体骨架---反向EN1靶位点---任意碱基---同源臂13R---正向EN1靶位点---载体骨架”排列，所述接受载体A的载体骨架中不含有EN1靶位点；

所述供给载体D1的多克隆位点中含有三组核酸内切酶识别靶位点，且从上游到下游按照：“载体骨架---正向EN1靶位点---反向EN3靶位点---任意碱基---正向EN3靶位点---反向EN2靶位点---任意碱基---正向EN2靶位点---同源臂13R---反向EN1靶位点---载体骨架”排列，所述供给载体D1的载体骨架中不含有EN1、EN2、EN3靶位点；

所述供给载体D2的多克隆位点中含有三组核酸内切酶识别靶位点，且从上游到下游按照：“载体骨架---正向EN2靶位点---反向EN3靶位点---任意碱基---正向EN3靶位点---反向EN1靶位点---任意碱基---正向EN1靶位点---同源臂13R---反向EN2靶位点---载体骨架”排列，所述供给载体D2的载体骨架中不含有EN1、EN2、EN3靶位点；

所述核酸内切酶为识别位点和酶切位点不完全重复的DNA内切酶。

进一步的，所述核酸内切酶的正向识别靶位点的酶切位点在识别靶位点的下游，反向识别靶位点的酶切位点在识别靶位点的上游。

进一步的，所述核酸内切酶为RNA介导的LbCas12a核酸内切酶。

所述LbCas12a核酸内切酶的crRNA分别为crRNA1、crRNA2、crRNA3；通过人工将crRNA1、crRNA2、crRNA3与LbCas12a蛋白融合，形成三种LbCas12a核酸内切酶，分别靶向对应的EN1、EN2、EN3靶位点；通过人工设计crRNA，其识别靶位点的序列长度可控，优选大于7bp，也可以是8bp或更长、9bp或更长、12bp或更长等；LbCas12a核酸内切酶识别的靶位点序列越长，目标大片段中存在2个或更多个该靶位点的概率越低，即酶切位点重复出现的概率越小，因此便于寻找合适的单一酶切位点，实现大片段的连续组装。

进一步的，所述核酸内切酶的识别靶位点长度大于等于12bp。

进一步的，所述EN1、EN2和EN3靶位点中含有限制性内切酶的酶切位点，所述EN1、EN2和EN3 EN1中限制性内切酶的酶切位点不同，且所述载体骨架不含有EN1、EN2、EN3靶位点以及该限制性内切酶的酶切位点。

进一步的，所述限制性内切酶为BsmBI、SapI和BsaI。例如：所述EN1靶位点含有BsmBI的酶切位点，所述EN2靶位点含有SapI的酶切位点，所述EN3靶位点含有BsaI的酶切位点。

采用上述的技术方案：在构建目的片段时可以根据实际情况选用酶切连接还是同源重组，以及是选用EN3酶切供给载体还是BsaI酶切供给载体，例如，供给载体D1在组装大片段seg1时，若seg1片段不含有BsaI的酶切位点，则seg1可以采用BsaI酶切，T4 DNAligase连接的方法构建；或者供给载体D1采用BsaI酶切，seg1采用同源重组克隆到供给载体D1，供给载体D1也可以采用EN3酶切；大大提高了系统的适用性。

进一步的，所述供给载体D1和供给载体D2的反向EN3靶位点和正向EN3靶位点之间添加负筛选基因或颜色显示基因，负筛选基因如CCDB致死基因，颜色显示基因如GFP，RFP等荧光基因或者花青素，胡罗卜素等颜色合成基因，便于筛选阳性克隆子，避免目标片段克隆进入时供给载体D1和供给载体D2出现假阳性，提高目标片段克隆进入供给载体的效率。

进一步的，所述接受载体A的反向EN1靶位点和正向EN1靶位点之间、所述供给载体D1的反向EN2靶位点和正向EN2靶位点之间以及所述供给载体D2的反向EN1靶位点和正向EN1靶位点之间添加颜色显示基因，颜色显示基因如GFP，RFP等荧光基因或者花青素，胡罗卜素等颜色合成基因，便于筛选。例如：所述接受载体A的反向EN1靶位点和正向EN1靶位点之间添加黄色色素蛋白基因(BBa_K592010amilGFP yellow)，所述供给载体D1的反向EN2靶位点和正向EN2靶位点之间添加蓝色色素蛋白基因(BBa_K592009amilCP blue)，所述供给载体D2反向EN1靶位点和正向EN1靶位点之间添加黄色色素蛋白基因(BBa_K592010amilGFPyellow)。

进一步的，所述接受载体A和供给载体D1、供给载体D2的抗性不同，所述供给载体D1和供给载体D2抗性相同。

本发明的目的二在于提供：一种采用上述用于大片段DNA无缝组装的系统的组装方法；包括以下步骤：

S1、构建载体系统；构建包括接受载体A和供给载体D1、供给载体D2的载体系统；

S2、目标片段克隆：将大片段DNA分成多个目标片段，并将目标片段按顺序依次交替克隆到供给载体D1和供给载体D2上，分别命名为：pBWRD1-seg1、pBWRD2-seg2、pBWRD1-seg3、pBWRD2-seg4、······、pBWRD1-segn-1、pBWRD2-segn；即将大片段分拆为seg1、seg2、seg3、seg4、······、segn-1、segn并依次组装到供给载体D1、D2中，得到供给载体D1-seg1、D2-seg2、D1-seg3、D2-seg4…D1-segn-1、D2-segn等，n＝1,2,3,4,5,6，······等自然数；其中，所述大片段DNA的片段长度为1kb或更长(例如，2kb或更长、2.5kb或更长、3kb或更长、5kb或更长、7.5kb或更长、10kb或更长、15kb或更长、20kb或更长、25kb或更长、40kb或更长、50kb或更长、75kb或更长或100kb或更长)；所述目标片段的片段长度为0.5kb或更长(例如，0.7kb或更长、1kb或更长、2kb或更长、2.5kb或更长、5kb或更长、7.5kb或更长、10kb或更长、15kb或更长、20kb或更长)；

S3、连续组装：用核酸内切酶EN1同时酶切接受载体A和酶切供给载体D1-seg1，通过组装反应将第一个目标片段装配到接受载体A中，得到pBWRA-seg1，然后采用核酸内切酶EN2同时酶切组装有第一个片段的pBWRA-seg1和酶切供给载体D2-seg2，通过组装反应将第二个片段装配到pBWRA-seg1中，得到pBWRA-seg1-seg2；

S4、重复步骤S3，根据确定的组装顺序将目标片段分步组装连接到接受载体A上，形成最终所需要的载体。

组装过程简述如下：

pBWRA+pBWRD1-seg1→pBWRA-seg1；

pBWRA-seg1+pBWRD2-seg2→pBWRA-seg1-seg2；

pBWRA-seg1-seg2+pBWRD1-seg3→pBWRA-seg1-seg2-seg3；

······

pBWRA-seg1-seg2-seg3-segn-1+pBWRD2-segn→pBWRA-seg1-seg2-seg3-segn-1-segn。

进一步的，所述组装方式为任何一种对具体序列不依赖的同源重组方法，如：Gibson Assembly。

本发明的多片段DNA分子无缝连续组装的原理为：首先用EN1酶切接受载体pBWRA和供给载体pBWRD1-seg1，获得线性化pBWRA和目标片段seg1(D1-seg1)，线性化pBWRA的切口两端的序列有10-50bp与从供给载体pBWRD1-seg1上切下来的D1-seg1的两端序列相同或者同源；这样D1-seg1可以通过与pBWRA的载体骨架进行同源重组的方式整合进接pBWRA中，得到pBWRA-seg1，同时将D1-seg1的EN2酶切位点带入到pBWRA-seg1上，同理通过EN2线性化载体pBWRA-seg1和获得目标片段seg2(D2-seg2)，线性化pBWRA-seg1的切口两端的序列有10-50bp与从供给载体pBWRD2-seg2上切下来的D2-seg2的两端序列相同或者同源；这样D2-seg2可以通过与pBWRA-seg1的载体骨架进行同源重组的方式整合进接pBWRA-seg1中，得到pBWRA-seg1-seg2，同时将D2-seg2的EN1酶切位点带入到pBWRA-seg1-seg2上，循环上述过程，即可实现将目标大片段DNA在目的载体pBWRA上实现无缝组装。

采用上述的技术方案：LbCas12a是一种RNA介导的核酸内切酶，其切割DNA的方式近似于Type IIs限制性内切酶，LbCas12a切割其靶向链的18～22bp，其互补链的26bp，本发明通过设计三个RNA融合LbCas12a以替代本实验室之前BioWalk1.0所使用的BsmBI、SapI、BsaI三种Type IIs限制性内切酶，使目标DNA分子含有LbCas12a核酸内切酶的酶切位点的概率为(4)²²/2*4*4*4*3＝1.7*10¹⁵bp＝1.7*10⁶Gbp，假设存在3个碱基的错配会导致错切，其酶切位点出现的概率也至少为2.6*10⁴Gbp，即2.6*10⁴Gbp才有一个酶切位点重复出现，而现存的基因组能遇到该靶点的概率几乎为零，因此通过人工设计的RNA介导的LbCas12a核酸内切酶不受酶切位点的限制，能够支持更大片段的连接，可以近乎无限的无缝连续组装出超大的DNA片段，能够应用于现有的合成生物学组装技术。

本发明的目的三在于提供：上述的一种用于大片段DNA无缝组装方法在合成生物学、大片段DNA克隆或多基因载体构建中的应用。

与现有技术相比，本发明的有益之处在于：

本发明通过人工设计的RNA介导的LbCas12a核酸内切酶的识别靶位点长度大于等于12bp，远大于现有技术中的Type IIs限制性内切酶，因此不受酶切位点的限制，能够支持更大片段的连接，适用于大片段DNA分段无缝组装成一个更大的完整片段，在含有多个基因的转基因载体构建、合成生物学、代谢通路研究等方面具有良好的应用前景。

附图说明

图1为本发明实施例1的接受载体A(pBWRA)、供给载体D1(pBWRD1)和供给载体D2(pBWRD2)的图谱；

具体实施方式

下面将结合本发明中的附图，对本发明的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动条件下所获得的所有其它实施例，都属于本发明保护的范围。

实施例1一种用于大片段DNA无缝组装的系统的建立

一种用于大片段DNA无缝组装的系统，包括由接受载体A(pBWRA，具体序列见SEQID NO:13)、供给载体D1(pBWRD1，具体序列见SEQ ID NO:14)和供给载体D2(pBWRD2，具体序列见SEQ ID NO:15)组成的系统；

1)接受载体pBWRA采用pBR322复制子，卡那霉素抗性，其图谱和多克隆位点如图1所示，图中，Kana^R:卡纳霉素抗性基因；ori来源于质粒pBR322的复制起始位点，用于在大肠杆菌中复制；bom来源于质粒pBR322的复制动员位点(basis of mobility)。所述接受载体A的多克隆位点中含有一组LbCas12a核酸内切酶识别靶位点EN1，该EN1靶位点中同时含有BsmBI酶切位点，且按照：“载体骨架---反向EN1靶位点---任意碱基或蓝色色素蛋白BBa_K592009amilCP blue---同源臂13R---正向EN1靶位点---载体骨架”排列，所述接受载体A的载体骨架中不含有EN1靶位点和BsmBI酶切位点；

2)供给载体pBWRD1的抗性基因为氨苄青霉素抗性基因，其图谱和多克隆位点如图1所示，图中，ori来源于质粒pBR322的复制起始位点，用于在大肠杆菌中复制；bom来源于质粒pBR322的复制动员位点(basis of mobility)。所述供给载体D1的多克隆位点中含有三组核酸内切酶LbCas12a识别靶位点，且从上游到下游按照：“载体骨架---正向EN1靶位点---反向EN3靶位点---意碱基或负筛选基因CCDB致死基因---正向EN3靶位点---反向EN2靶位点---任意碱基或蓝色色素蛋白BBa_K592009amilCP blue---正向EN2靶位点---同源臂13R---反向EN1靶位点---载体骨架”排列，其中所述EN1靶位点含有BsmBI的酶切位点，所述EN2靶位点含有SapI的酶切位点，所述EN3靶位点含有BsaI的酶切位点；具体各靶位点的序列信息如下：所述EN1靶位点的序列为：tttaggtgtaacgtgatgacgtctcg(SEQ ID NO:4)；所述EN2靶位点的序列为：tttcgcagcatctaacgagctcttcg(SEQ ID NO:5)，所述EN3靶位点的序列为：tttcgaggacgagattcagggtctca(SEQ ID NO:6)。且所述pBWRD1的载体骨架中不含有EN1、EN2、EN3靶位点以及BsmBI、SapI、BsaI的酶切位点。

3)供给载体pBWRD2的抗性基因为氨苄青霉素抗性基因，其图谱和多克隆位点如图1所示，图中，ori来源于质粒pBR322的复制起始位点，用于在大肠杆菌中复制；bom来源于质粒pBR322的复制动员位点(basis of mobility)。所述供给载体D2的多克隆位点中含有三组核酸内切酶LbCas12a识别靶位点，且从上游到下游按照：“载体骨架---正向EN2靶位点---反向EN3靶位点---任意碱基或负筛选基因CCDB致死基因---正向EN3靶位点---反向EN1靶位点---任意碱基或黄色色素蛋白BBa_K592010amilGFP yellow---正向EN1靶位点---同源臂13R---反向EN2靶位点---载体骨架”排列，其中所述EN1靶位点含有BsmBI的酶切位点，所述EN2靶位点含有SapI的酶切位点，所述EN3靶位点含有BsaI的酶切位点；具体各靶位点的序列信息如下：所述EN1靶位点的序列为：tttaggtgtaacgtgatgacgtctcg(SEQ IDNO:4)；所述EN2靶位点的序列为：tttcgcagcatctaacgagctcttcg(SEQ ID NO:5)，所述EN3靶位点的序列为：tttcgaggacgagattcagggtctca(SEQ ID NO:6)。且所述pBWRD2的载体骨架中不含有EN1、EN2、EN3靶位点以及BsmBI、SapI、BsaI的酶切位点。

所述LbCas12a核酸内切酶的正向识别靶位点的酶切位点在识别靶位点的下游，反向识别靶位点的酶切位点在识别靶位点的上游。

所述LbCas12a核酸内切酶的crRNA分别为crRNA1、crRNA2、crRNA3；通过人工将crRNA1、crRNA2、crRNA3与LbCas12a蛋白融合，形成三种LbCas12a核酸内切酶，分别靶向对应的EN1、EN2、EN3靶位点。各crRNA的序列信息如下：所述crRNA1的序列为UAAUUUCUACUaagUGUAGAUGGUGUAACGUGAUGACGUCUCG(SEQ ID NO:1)，crRNA2的序列为UAAUUUCUACUaagUGUAGAUGAGGACGAGAUUCAGGGUCUCA(SEQ ID NO:2)，crRNA3的序列为UAAUUUCUACUaagUGUAGAUGCAGCAUCUAACGAGCUCUUCG(SEQ ID NO:3)。上述提供的crRNA的序列信息仅为示例性，并不应作为对本发明保护范围的限定，本领域技术人员应该知道，任何只要能够实现crRNA1、crRNA2、crRNA3靶向EN1、EN2、EN3靶位点的序列都属于本发明的保护范围。

实施例2一种采用实施例1的用于大片段DNA无缝组装的系统进行多基因组装

S1、构建载体系统；构建包括接受载体A和供给载体D1、供给载体D2的载体系统；

S2、目标片段克隆：从BAC载体上将水稻基因LOC_Os04g53120、LOC_Os04g53130、LOC_Os04g53140、LOC_Os04g53150、LOC_Os04g53160、LOC_Os04g53170、LOC_Os04g53180、LOC_Os04g53190、LOC_Os04g53195共9个基因约44kb的序列(序列信息见NCBI公开序列，链接为：https://www.ncbi.nlm.nih.gov/nucleotide/NW_015379177.1？report＝genbank&log$＝nuclalign&blast_rank＝1&RID＝1V8UUZN3016&from＝31632812&to＝31676812)组装到pBWRA载体上转基因表达。

S21、首先将44kb的序列分成三段约15kb的序列(seg1-3)，排列如下：AGCTTCCCGGGGCGCTTATCTCGTG---seg1---CAGTCCATTACGGACAAGAAGC---seg2---ACACATTAACCATTATAAGAGCTCATCTC---seg3---GCGCGCGTAGTTAATCTGTCAA；

S22、用高保真酶BioPfu扩增出seg1-3，并用EN3酶切供给载体D1、D2；通过同源重组将目标片段seg1、seg2、seg3分别构建到供给载体D1、D2、D1中，将供给载体D1和供给载体D2的“---反向EN3靶位点---任意碱基---正向EN3靶位点---”替代下来，得到载体pBWRD1-seg1(具体序列见SEQ ID NO:16)、pBWRD2-seg2(具体序列见SEQ ID NO:17)、pBWRD1-seg3(具体序列见SEQ ID NO:18)，并测序验证。

pBWRD1-seg1从上游到下游按照：“载体骨架---正向EN1靶位点---seg1---反向EN2靶位点---任意碱基或蓝色色素蛋白BBa_K592009amilCP blue---正向EN2靶位点---同源臂13R---反向EN1靶位点---载体骨架”排列。

pBWRD2-seg2从上游到下游按照：“载体骨架---正向EN2靶位点---seg2---反向EN1靶位点---任意碱基或黄色色素蛋白BBa_K592010amilGFP yellow---正向EN1靶位点---同源臂13R---反向EN2靶位点---载体骨架”排列。

pBWRD1-seg3从上游到下游按照：“载体骨架---正向EN1靶位点---seg3---反向EN2靶位点---任意碱基或蓝色色素蛋白BBa_K592009amilCP blue---正向EN2靶位点---同源臂13R---反向EN1靶位点---载体骨架”排列。

构建pBWRD1-seg1的引物为：

D1-seg1f:GTAACGTGATGACGTCTCGAAACCGCCTGCAGGTCTAGAGCTTCCCGGGGCGCTTATC(SEQ ID NO:7)

D1-seg1r:CGCAGCATCTAACGAGCTCTTCGGGGCCGCGCCCTCGGGCTTCTTGT(SEQ ID NO:8)

构建pBWRD2-seg2的引物为：

D2-seg2f:CAGCATCTAACGAGCTCTTCGCAGTCCATTACGGACAAGAAGC(SEQ ID NO:9)

D2-seg2r:GGTGTAACGTGATGACGTCTCGCTTGGTTGCTATTAGCTTATGTGTGAG(SEQ ID NO:10)

构建pBWRD1-seg3的引物为：

D1-seg3f:GTGTAACGTGATGACGTCTCGACACATTAACCATTATAAGAGCTCATCTC(SEQ IDNO:11)

D1-seg3r:CGCAGCATCTAACGAGCTCTTCGTTGACAGATTAACTACGCGCGC(SEQ ID NO:12)

S3、连续组装：将核酸内切酶EN1酶切获得的线性化pBWRA和酶切pBWRD1-seg1获得的目标片段D1-seg1，通过同源重组得到载体pBWRA-seg1，仅需酶切验证无需测序。pBWRA-seg1从上游到下游按照：“载体骨架---seg1---反向EN2靶位点---任意碱基或蓝色色素蛋白BBa_K592009amilCP blue---同源臂13R---正向EN2靶位点---载体骨架”排列。然后采用核酸内切酶EN2酶切pBWRA-seg1，获得线性化pBWRA-seg1和酶切pBWRD2-seg2获得目标片段D2-seg2，通过同源重组将第二个片段D2-seg2装配到pBWRA-seg1中，得到pBWRA-seg1-seg2，仅需酶切验证无需测序。pBWRA-seg1-seg2从上游到下游按照：“载体骨架---seg1---seg2---反向EN1靶位点---任意碱基或黄色色素蛋白BBa_K592010amilGFP yellow---正向EN1靶位点---同源臂13R---载体骨架”排列；

S4、重复步骤S3，根据确定的组装顺序将目标片段分步组装连接到接受载体A上，形成最终所需要的载体。采用核酸内切酶EN1酶切pBWRA-seg1-seg2，获得线性化pBWRA-seg1-seg2和酶切pBWRD1-seg3获得的目标片段D1-seg3，通过同源重组将第三个片段D1-seg3装配到pBWRA-seg1-seg2中，得到pBWRA-seg1-seg2-seg3(具体序列见SEQ ID NO:19)，仅需酶切验证无需测序。pBWRA-seg1-seg2-seg3从上游到下游按照：“载体骨架---seg1---seg2---seg3---反向EN2靶位点---任意碱基或蓝色色素蛋白BBa_K592009amilCP blue---同源臂13R---正向EN2靶位点---载体骨架”排列。

序列表

<110> 湖北伯远合成生物科技有限公司

<120> 一种用于大片段DNA无缝组装的系统及组装方法

<150> 2019114206233

<151> 2019-12-31

<160> 19

<170> SIPOSequenceListing 1.0

<210> 1

<211> 43

<212> RNA

<213> 人工序列(Artificial Sequence)

<400> 1

uaauuucuac uaaguguaga ugguguaacg ugaugacguc ucg 43

<210> 2

<211> 43

<212> RNA

<213> 人工序列(Artificial Sequence)

<400> 2

uaauuucuac uaaguguaga ugaggacgag auucaggguc uca 43

<210> 3

<211> 43

<212> RNA

<213> 人工序列(Artificial Sequence)

<400> 3

uaauuucuac uaaguguaga ugcagcaucu aacgagcucu ucg 43

<210> 4

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 4

tttaggtgta acgtgatgac gtctcg 26

<210> 5

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 5

tttcgcagca tctaacgagc tcttcg 26

<210> 6

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 6

tttcgaggac gagattcagg gtctca 26

<210> 7

<211> 58

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 7

gtaacgtgat gacgtctcga aaccgcctgc aggtctagag cttcccgggg cgcttatc 58

<210> 8

<211> 47

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 8

cgcagcatct aacgagctct tcggggccgc gccctcgggc ttcttgt 47

<210> 9

<211> 43

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 9

cagcatctaa cgagctcttc gcagtccatt acggacaaga agc 43

<210> 10

<211> 49

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 10

ggtgtaacgt gatgacgtct cgcttggttg ctattagctt atgtgtgag 49

<210> 11

<211> 50

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 11

gtgtaacgtg atgacgtctc gacacattaa ccattataag agctcatctc 50

<210> 12

<211> 45

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 12

cgcagcatct aacgagctct tcgttgacag attaactacg cgcgc 45

<210> 13

<211> 9013

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 13

tagaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg 60

ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg 120

gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga 180

caacttaata acacattgcg gacgttttta atgttagact gaattaacgc cgaattaatt 240

cgggggatct ggattttagt actggatttt ggttttagga attagaaatt ttattgatag 300

aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga 360

aaccctatag gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa 420

ctcgagcttg tcgatcgaca gatccggtcg gcatctactc tatttctttg ccctcggacg 480

agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac 540

ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat 600

tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg 660

atagagttgg tcaagaccaa tgcggagcat atacgcccgg agtcgtggcg atcctgcaag 720

ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct 780

ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc 840

aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg 900

cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg 960

cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc 1020

agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa 1080

tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tagcctccgc 1140

gaccggttgt agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg 1200

tgcacggcgg gagatgcaat aggtcaggct ctcgctaaac tccccaatgt caagcacttc 1260

cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc 1320

atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc 1380

acgagattct tcgccctccg agagctgcat caggtcggag acactgtcga acttttcgat 1440

cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatctc attgcccccc 1500

cggatctgcg aaagctcgag agagatagat ttgtagagag agactggtga tttcagcgtg 1560

tcctctccaa atgaaatgaa cttccttata tagaggaagg tcttgcgaag gatagtggga 1620

ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt 1680

ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc 1740

actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta 1800

ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa 1860

tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc 1920

tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttatc 1980

acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct 2040

cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt 2100

tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat 2160

gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg 2220

aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac 2280

gagagtgtcg tgctccacca tgttggcaag ctgctctagc caatacgcaa accgcctgca 2340

ggtctagcga gacgtcatca cgttacacct aaaatgtagg ctataccacg cgttaactag 2400

ccgaggccag acgtttgtac gccaaggtaa tgatttaggt gtaacgtgat gacgtctcgg 2460

tcgcagtcat aacttcgtat agcatacatt gtcgagcttg gcactggccg tcgttttaca 2520

acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 2580

tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 2640

cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg tttcccgcct 2700

tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag 2760

cgtttattag aataacggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt 2820

gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc 2880

cgctgctata gtgcagtcgg cttctgacgt tcagtgcagg agatgatcgc ggccgggtac 2940

gtgttcgagc cgcccgcgca tgtctcaacc gtgcggctgc atgaaatcct ggccggtttg 3000

tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc 3060

cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat 3120

atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac 3180

ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc 3240

aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt 3300

gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg 3360

accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg 3420

cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc 3480

caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg 3540

aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc 3600

gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc 3660

gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat 3720

cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa 3780

aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc 3840

cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc 3900

agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta 3960

cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc 4020

agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca 4080

tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg 4140

gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc 4200

ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg gcccggtaca aatcggcgcg 4260

gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc 4320

atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa 4380

gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac 4440

gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc 4500

atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc 4560

cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt 4620

gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga 4680

taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta 4740

ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc 4800

attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc 4860

ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa 4920

accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca 4980

gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc 5040

atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg 5100

ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc 5160

accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg 5220

gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc 5280

gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt 5340

cgaaaagatc tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg 5400

aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa 5460

gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt 5520

aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagct 5580

cccggatacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 5640

cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag 5700

cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat 5760

atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgttcatcc 5820

gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 5880

cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 5940

tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 6000

cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 6060

aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 6120

cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 6180

gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 6240

ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 6300

cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 6360

aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 6420

tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 6480

ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 6540

tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 6600

ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 6660

cattctaggg aaggtgcgaa caagtccctg atatgagatc atgtttgtca tctggagcca 6720

tagaacaggg ttcatcatga gtcatcaact taccttcgcc gacagtgaat tcagcagtaa 6780

gcgccgtcag accagaaaag agattttctt gtcccgcatg gagcagattc tgccatggca 6840

aaacatggtg gaagtcatcg agccgtttta ccccaaggct ggtaatggcc ggcgacctta 6900

tccgctggaa accatgctac gcattcactg catgcagcat tggtacaacc tgagcgatgg 6960

cgcgatggaa gatgctctgt acgaaatcgc ctccatgcgt ctgtttgccc ggttatccct 7020

ggatagcgcc ttgccggacc gcaccaccat catgaatttc cgccacctgc tggaacagca 7080

tcaactggcc cgccaattgt tcaagaccat caatcgctgg ctggccgaag caggcgtcat 7140

gatgactcaa ggcaccttgg tcgatgccac catcattgag gcacccagct cgaccaagaa 7200

caaagagcag caacgcgatc cggagatgca tcagaccaag aaaggcaatc agtggcactt 7260

tggcatgaag gcccacattg gtgtcgatgc caagagtggc ctgacccaca gcctggtcac 7320

caccgcggcc aacgagcatg acctcaatca gctgggtaat ctgctgcatg gagaggagca 7380

atttgtctca gccgatgccg gctaccaagg ggcgccacag cgcgaggagc tggccgaggt 7440

ggatgtggac tggctgatcg ccgagcgccc cggcaaggta agaaccttga aacagcatcc 7500

acgcaagaac aaaacggcca tcaacatcga atacatgaaa gccagcatcc gggccagggt 7560

ggagcaccca tttcgcatca tcaagcgaca gttcggcttc gtgaaagcca gatacaaggg 7620

gttgctgaaa aacgataacc aactggcgat gttattcacg ctggccaacc tgtttcgggc 7680

ggaccaaatg atacgtcagt gggagagatc tcactaaaaa ctggggataa cgccttaaat 7740

ggcgaagaaa cggtctaaat aggctgattc aaggcattta cgggagaaaa aatcggctca 7800

aacatgaaga aatgaaatga ctgagtcagc cgagaagaat ttccccgctt attcgcacct 7860

tccctaggta ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag 7920

gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc 7980

tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa 8040

gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc 8100

cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc 8160

gcggatcttt aaatggagtg tcctcttccc agttttcgca atccacatcg gccagatcgt 8220

tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat 8280

ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt 8340

cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga 8400

gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc 8460

cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc 8520

ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta tataccttag 8580

caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt 8640

ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa 8700

gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta 8760

aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat 8820

agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg 8880

ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt 8940

gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc 9000

ccgctgacgc cgt 9013

<210> 14

<211> 2570

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 14

cacatttccc cgaaaagtgc cacctgacgt ctttaggtgt aacgtgatga cgtctcgtga 60

gaccctgaat ctcgtcctcg aaaactgtcc gtcagatgtt gatcggcacg taagaggttc 120

caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt 180

ttcaggagct aaggaagcta aacttttgct gacgagaaca gggactggtg aaatgcagtt 240

taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga 300

tattattgac acgcctgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc 360

agataaagtc tcccgtgaac tttacccggt ggtgcatatc gaggatgaaa gctggcgcat 420

gatgaccacc gatatggcca gtgtgccggt atccgttatc ggggaagaag tggctgatct 480

cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat 540

gtcaggctcc cttatacaca ggtcgacggt ctgactgact ttcgaggacg agattcaggg 600

tctcacgaag agctcgttag atgctgcgaa aatgtaggct ataccacgcg ttaactagcc 660

gaggccagac gtttgtacgc caaggtaatg atttcgcagc atctaacgag ctcttcggtc 720

gcagtcataa cttcgtatag catacattgt cgcgagacgt catcacgtta cacctaaaaa 780

gcttacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 840

gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 900

aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 960

gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 1020

ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 1080

cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 1140

ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 1200

actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 1260

tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 1320

gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 1380

ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 1440

cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 1500

ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 1560

tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 1620

agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 1680

gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 1740

ccgcgagaac cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 1800

gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 1860

cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 1920

acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 1980

cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 2040

cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 2100

ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 2160

tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 2220

atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 2280

tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 2340

actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 2400

aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 2460

ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 2520

ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 2570

<210> 15

<211> 2570

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 15

cacatttccc cgaaaagtgc cacctgacgt ctttcgcagc atctaacgag ctcttcgtga 60

gaccctgaat ctcgtcctcg aaaactgtcc gtcagatgtt gatcggcacg taagaggttc 120

caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt 180

ttcaggagct aaggaagcta aacttttgct gacgagaaca gggactggtg aaatgcagtt 240

taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga 300

tattattgac acgcctgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc 360

agataaagtc tcccgtgaac tttacccggt ggtgcatatc gaggatgaaa gctggcgcat 420

gatgaccacc gatatggcca gtgtgccggt atccgttatc ggggaagaag tggctgatct 480

cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat 540

gtcaggctcc cttatacaca ggtcgacggt ctgactgact ttcgaggacg agattcaggg 600

tctcacgaga cgtcatcacg ttacacctaa aatgtaggct ataccacgcg ttaactagcc 660

gaggccagac gtttgtacgc caaggtaatg atttaggtgt aacgtgatga cgtctcggtc 720

gcagtcataa cttcgtatag catacattgt cgcgaagagc tcgttagatg ctgcgaaaaa 780

gcttacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 840

gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 900

aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 960

gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 1020

ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 1080

cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 1140

ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 1200

actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 1260

tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 1320

gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 1380

ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 1440

cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 1500

ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 1560

tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 1620

agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 1680

gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 1740

ccgcgagaac cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 1800

gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 1860

cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 1920

acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 1980

cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 2040

cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 2100

ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 2160

tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 2220

atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 2280

tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 2340

actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 2400

aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 2460

ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 2520

ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 2570

<210> 16

<211> 18515

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 16

cacatttccc cgaaaagtgc cacctgacgt ctttaggtgt aacgtgatga cgtctcgagc 60

ttcccggggc gcttatctcg tgcggcgtcc tccaagccgc ggctgcactc tccctcgtct 120

tcttcatcgc tccgagcgga gtcttcggca accatggtaa ggcactccac tggctgtact 180

acggaagcct tgtcaccgtg gtgctcgtcg ggtttgtaga ggcttctgtc gggttttggg 240

tggcaggtga tctggcaaac cggcgagcag tgggcaggac gatgctcttg gtttgcctct 300

tcagcctgat cttcgtggtc actctttggg gctctgttct tgtgagatga ttgcattctt 360

tgctgtatgt acaagattta gcattccttt gtgagtatgt ggtatttttc aactgtgttg 420

tactgttgtc taaatatatt cattgtaata ttcaaactgt gtgagatttt catttgtagt 480

ttcagaaatt cttggacgat ttttaccgta cttttgattt gggctgtttt cgtgtaatta 540

gtactactag tatttacgtt gtcttttcag acgatcgcag ttctgacatg tgcttacgct 600

gcctctattc tacttccgat agcgctgcac atatttgcac tcgcgttcag aaggcaaatg 660

cagcaaacaa gttgtaatgg ggttcagcac ttcagcctct tctaaatata tccagcactg 720

cagcatgctg aaacttctcc ccttccagtc caccaagatt tcaacttcaa attttgttca 780

tacttaactc aaaatttcac gaactgatca ccctcgagtc gaaaattttc aatccacatg 840

caaacttctg tagctttcac tagtctacac taatataaaa agcaccagtt catcaaaccc 900

aaatattttc tgacctttag atctattcat ccaatggctc agatcaagag ttggatcaac 960

ctctacaatt agcgattcca cgtcgtgtaa gtagcggtta caaaaattaa ttagcgatca 1020

tcaaacaaaa agaaaaaaga agaagttatt agtgatccat ctcccactcc cctaccctgc 1080

cgcctccgcc agtccgcctc cttgcgccta ccccaccgcc gtcgcccgcc gccttctcca 1140

ccagcgccga cgcctcctca cgcctacccc attgccgtcg ctgtcgtcgg ccccgccttc 1200

tccgccggcg gcagccgcga aacctacccc gccgccgacg tctcctcgcg cctaccaccc 1260

cctactctca cgctccgtcg ccgatctcct tcgccgttgc cccgccatcg atctccgacg 1320

ccggaactcc gttgcatgtc gcacgtcacc cagatcggcg tcgctccaag attggcgtca 1380

cggtcgcggg cgtcgatctc gctcccctat cggcatcgca atcactggcg atgatatcgt 1440

ggcctcgaac cttcgtagct gtctccatcg ccttcgccgc tgctgatcct gcttccccag 1500

ttcgccgtcg tcgccttcga tccggcgggc cacgcctctg tccacgccca ccgcggagtg 1560

cgcccctcca gccagtacag cagcatcgcc actgccgaca ggagcagcat ccccgtccag 1620

attgtcagca gacaaccagg tgattggatt ggttttcttt tatttggttg gattctgcat 1680

tttgaacgaa agcagtaaaa ttcatcattc tttgatttgg ttggattcag aattgacaat 1740

acatcataaa tctgagtaaa tcattaagca atattacatt ctctattatt taattattgc 1800

aattgttaat catgcttaaa aaacataatg ttattcatgc tcaaccataa tgctggcaac 1860

taaaaataat acctagattg acagtagtac agttgaacaa tacctagact gaccgtagta 1920

cagttgggtg gcgaaggtct cattgctcac tgatggcttt ctaaatcccc tattccgtcg 1980

taagcctgat tatcgtggta tattatttct tggaggtgtt ataggtgata tgaatggaag 2040

ggcatgtaca ctgttatagc aggaataaaa cctaggcttg atgctaattt acatatgaag 2100

aacacgcaaa ttaatttaat taattgttat gctttattct gcttgtttct atgcactaca 2160

attccaattt gagtgcctat cttgtcatgc tattcagtat aatgtcttgg caggatatga 2220

atctgttcat gcatctatat acagccttca tataattaag taaggattaa ccagattact 2280

tgctttgtct aatgcagcct caccatttca tttgtggcga gcaccttaat tccatcttgc 2340

cattgtcaag agtatatcat ctgcatctaa ttctgtatat tgagtatata ttgactagga 2400

tcttggaaat ttttgaattg ttgtcagata ttaagttcct tagtttgatg atttgattag 2460

ttttgcaact aatcaccatt gatcacaagt aaacaccact tatagcagtt taatggctat 2520

ttatctaaaa aggaaaaaac tagaagttta atgactgaaa ctgcagtggt actatgatct 2580

ataaacaaga aatctgctga gtttcctagc agttgttggg tgcactttcc tcttgctgtg 2640

tcatccatta aatttcaaaa actgcaaatt ctttgtatag gtattactgg gactcaaatg 2700

tacacctttc agttcacact caattttgta gaacagtaaa agcgtgaaaa tataggaaat 2760

ataattgatt tgttcagaaa attattcaaa tgctaacaca ctgcctaggt aatcactttc 2820

tcaacataag tatatgtaca acaatgcata ttgtttctac ttttcacaat tgggaacctg 2880

aatacataag tatatgtaca acaatgcata ttgtttctac ttttcacaat tgggaacctg 2940

aaatatcaag attctaaaac taatgtgtgt ctgctgtaca gttcttaaaa caatgctatg 3000

gaatattcta tgactaatat gttgcactgt agagttctga agattcttca atcaaagcgt 3060

gggaaacttc tgagggaaag tttgtcaaaa aaattcaggt taccagtgtt ttggtggaca 3120

tttacatact tatgcaattt gatatgctaa atgaatttac tactaataca ttacatcaat 3180

cttctgtttg taactggttc aatggaccat tagagctttc tgttgacaaa ttaaatgagc 3240

tttgttcatt tatccaacaa cttttctttt tctgaatctt gctccaaatt ttgtcatatt 3300

attgactatt atttttttta aaccatgcaa ataatttgtt gatataacga tgttcacttg 3360

gcataagaaa tcatagcagt ggaggtaatt ttttttctct ttccactctg gaaacccatg 3420

cccctttttt tttcctgatt cattacaagt gacattactg tgcaatcttg caactgtttc 3480

acagatgcaa cccttagtgc tttgaataat ataataagat aatgattccc ttttagctgt 3540

tctatgtgca gcaaatatta ttattggctc cgttcactat ttggtagatt gtttcccatt 3600

tcgttggaca accagaacaa caatcgattg tatgatttgt atcttagtcc tttgcaagta 3660

aggtattgag aaaatgacat aatgggaaca gaggaagtga tgtaattctg taccttattt 3720

gtgaacctta atgtatacat aatatttatt tcaggaagac gggactgaat acatatttct 3780

taattccaaa ggcattcagg atgtttatga aagataatta ctgttgtttt taggaaacat 3840

attacatcgg cgttgtcagg taagctaaca tagagtgatg atgttcatgg tttcaaatga 3900

gaatatatat attcctcgca tggacaactt tactatgctt tgtcaaggag tatgtaaggc 3960

aaacagtaac agggattttt gccaaactac caaactagga aagagataga ccttaccgaa 4020

aatattatgt ataaaggttt cttggaatat tagaaaaata taatgacacc gtagcgttag 4080

cacgggcata ttactagtag aacaaaagaa acacttatgc atttataccc aaaaaaaacc 4140

gtaattaatg gaccgcattt cgtactgtgt agcggcccgt cgaagcataa ctcaaacttt 4200

taagccagag atgggcctgg gccaacatcg acataaactg gaataagtcc acttgccttc 4260

cctcaacttt acatggagtg tgtatgatgg ccctaattcc caataccaga tgtcgacccc 4320

tcaagtcttc aaaaccatgc aaagtagttc ctcgagggta tagaccatat ttcccctggt 4380

ttccattgat gtggcatata tcagggccca ctacctgaac catcgccacg tccagtcctt 4440

tcggcctgtt cgcgatgacg agctcggcca ccacctcaac atcgtccatg ctgcggcgat 4500

ggcgaggaga cagctgggac ggtgaacctc atagctccgt ctgttgcaac atcctctcgc 4560

catgctcacg ccgccacgac acccttgtcc tcctgctccg tagcagcctc gcaagctcac 4620

gccgcggcct catcgaggtc ccctgtcccc gagccgcttc tagcatcgat gagctgagga 4680

gctcccccac tgccgaggcg atgacgattt ggcagggagg gctcgattcg cccactccaa 4740

ttcgcagcgt cacacctccc ctcccccctc ctattcatcc accaccaacg cggataggag 4800

aagggataga ggaaggtgtg cgtggacact tacatgttgg ttccatattt tttttttact 4860

cggatgccac gtcagataaa acaaccgtcg gtcaacattt ttcgtagtgg ggattaggga 4920

tgtcatacat gactcgacgc agagctgagg gatggcaagt gaacttattc ccataaactt 4980

ccaaaagttt ggaatgctca acttaggctt ctcttcggat gctgaagtgc taatgggcca 5040

aataaccaac tagcccacag cccatcgaag tggaagccga cgagtccacc gtcaacgcgg 5100

cagtagtaat ccgagaaaac gcgggtcgcg cgaagcaatc gatcatcatc agacgattaa 5160

tccacgacga ccaggagcgc tgccccgagc cacacgccca cacgcactgg tgaatcttct 5220

cctttccacc tagttttcac tctgcagtct cctctcctag ctactactct gccttcccat 5280

cctcgcttgt tgcagccctc ttgcacacgc cattggccac agtccaagga ctgctcctgt 5340

tccgatggag gaggtggaag ccggttggct ggagggcggg atcaggtggc tggcggagac 5400

catcctggat aacctggacg ccgacaagct ggatgaatgg attcgccaga ttaggctcgc 5460

cgctgacacc gagaagctac gggctgagat cgagaaggtg gatggggtgg tggctgccgt 5520

gaaggggagg gcgatcggga acaggtcgct ggcccgatcg ctcggccgtc tcagggggtt 5580

gctgtacgac gccgacgatg cggtcgacga gctcgactac ttcaggctcc agcagcaggt 5640

cgagggagga ggtactgtct ttgcatatat ccgtgccttt taattaagtt tgcaagctgc 5700

gttgcctgca acaatggcgt attggcgtca gtttccaatc catgcttgtg ctacagttac 5760

tacacggttt gaggctgaag agacggtcgg agatggagca gaggacgagg acgatattcc 5820

gatggacaat actgatgtac cggaggcagt ggcggcaggc agcagcaaga aacggtccaa 5880

ggcatgggaa cactttacta ccgtagagtt cactgctgac gggaaggatt ctaaagcacg 5940

gtgcaagtac tgccacaagg acctatgttg cacatctaag aacgggacat cagctttgcg 6000

caaccatctc aatgtttgca agaggaaacg tgtaacaagt actgaccaac cggtaaatcc 6060

atcaaggtaa tgctaatgga gttctgaatt tagtgtaaat ccgttgaagt gtaaatttgg 6120

cccgttacat ctgcttaaga tctcattctg tctctaatct tctaatagcc aactcatggt 6180

catttttttt cctaatatat agtaccggtg atggtgcacc aaatgtaatt agatgcaagg 6240

aaacaaaagt gaacaattgt atatatcaaa tataattata tctaaaacat gagtagtgta 6300

tcaaatccaa ttctttcaaa aatctactat gcaaaattga gtgacaaaat ctgctgcctt 6360

ttttttttta cagaaagcaa ccaattaata taagtcaaat ataaaaacgc tttgtagtct 6420

ccaataaaat agctcattgt ttcgtttata cttatgttta taaatttaaa tttaaaactt 6480

aattttggag ttgattttgt ggttttcttt tcatcctatt ttattttaca acatttgatt 6540

ttgaatagtt aagaatgcgt atataaaaat tttacccata agttattttt taaattgtta 6600

ataaatcgta aggataatca taagtataag tgaaatgatt cgctcttcat ctacttaaga 6660

ttgcgttata ttgctgacct ttctaatcgc ctaaccacga tcacatgctc ttccagtgcc 6720

ggtgagggtg catcaaatgc aactggtaat tcagttggca gaaaaaggat gagaatggat 6780

gggacttcaa cacaccacga ggcagttagc acgcaccctt ggaacaaggc tgaactttcc 6840

aacaggatcc aatgcatgac tcatcagtta gaagaggctg taaatgaggt tatgaggcta 6900

tgtcgatcct caagttcaaa ccagagtcga cagggtacac caccggccac aaatgcaaca 6960

acatcgtctt atcttccgga gcccatagtg tatgggaggg ctgcagagat ggaaaccatc 7020

aaacagctga tcatgagcaa tagatctaat ggcataaccg tcctgccaat tgtaggcaat 7080

ggagggatag gaaaaaccac tttggcgcaa ctggtctgca aagatctggt aattaaaagt 7140

cagtttaatg ttaagatatg ggtgtatgta tctgataaat ttgatgtagt taagattaca 7200

aggcagattt tggatcatgt ctccaaccag agccacgaag gaataagcaa ccttgatacg 7260

cttcagcagg atcttgagga acaaatgaaa tctaagaagt tcctcattgt cttagatgat 7320

gtgtgggaaa tccgtacaga tgactggaaa aaactactgg ctcctttaag acctaatgat 7380

caggtgaatt cgtcacagga agaggcaaca ggtaatatga taattttgac aactcgtata 7440

cagagtattg ccaaaagtct tggaacagta caatcaatta agttagaagc tctgaaagat 7500

gacgatatat ggtcactatt taaagtgcat gcttttggta atgataaaca tgatagtagt 7560

ccaggcttac aggttcttgg gaagcaaatt gctagcgagc taaaaggcaa cccactggca 7620

gcaaaaactg tgggttcact attaggaacg aatcttacca tcgatcattg ggatagcatt 7680

ataaagagtg aagaatggaa atccctgcaa caagcttatg gcatcatgca agcgctgaag 7740

ttgtgctatg atcatctatc caacccctta cagcaatgcg tctcttattg ttctcttttc 7800

cccaagggtt attctttcag caaagcacaa ctaatacaaa tatggattgc tcaaggattt 7860

gtggaagaat ccagtgagaa gttggagcag aaaggatgga aatatctagc tgagttggta 7920

aattcgggtt tccttcagca agttgaaagc acacggtttt catcagaata ttttgttgtg 7980

cacgatctta tgcatgattt agcgcaaaag gtttcacaaa cagaatatgc aactatagat 8040

ggctcagagt gcacagagtt agccccaagt atacgccatt tgtcaatagt aactgattct 8100

gcataccgca aggagaaata tagaaacata tctcgtaatg aggtgtttga gaaaaggttg 8160

atgaaagtta agtcaaggag taagttgagg tcactggtat taattgggca atatgattct 8220

cattttttta aatatttcaa agatgctttc aaggaagcac aacatctgcg actgctgcag 8280

atcactgcaa cttatgctga ttctgattca tttctctcca gtttggtaaa ttctacacat 8340

ctccggtatc tgaaaattgt gactgaagaa tccggcagaa ctttgccccg atctctaagg 8400

aagtattacc atcttcaagt actagatatt ggctatagat ttggaattcc ccgtatatct 8460

aatgatataa ataatcttct cagcctgcgg catcttgttg catatgatga agtgtgttct 8520

tccattgcta acattggtaa aatgacctca cttcaggaac taggcaattt tattgttcag 8580

aataatttaa gtggttttga ggtgacacaa ttgaaatcca tgaacaagct tgtacaactt 8640

agtgtgtctc aacttgaaaa tgttagaact caggaggagg catgtggggc aaaactgaaa 8700

gacaaacaac acttagaaaa gctacatttg tcctggaagg atgcatggaa tggatatgac 8760

agtgacgaaa gctatgaaga tgaatacggc agtgatatga atatagaaac agaaggggag 8820

gaactgtcag ttggtgatgc caatggtgcc caaagcttac aacatcacag taatataagc 8880

tctgaacttg cttcaagtga ggtgctcgaa ggtcttgaac cacatcacgg cctcaagtat 8940

ctacggatat ctgggtataa tggatctacc tccccaactt ggcttccttc ttcacttacc 9000

tgtctgcaaa cacttcatct agaaaaatgt ggaaaatggc aaatacttcc tttagaaagg 9060

ctagggttac ttgtaaagct cgtgttgatc aaaatgagga atgcaacaga actctcaatc 9120

ccttcactgg aggagcttgt gttaattgca ttgccaagct tgaacacatg ctcctgcact 9180

tccatcagga acttgaactc cagtttaaag gttctgaaaa ttaagaattg ccctgtactg 9240

aaggtatttc ccttgtttga gatttgccag aaatttgaaa tcgagcggac gtcgtcatgg 9300

ttgccccatc ttagcaagct taccatctat aattgtcctc tttcctgtgt gcacagttct 9360

ctgccacctt catctattgt ttccaaatta tcgatcggta aagtttcaac acttccaacg 9420

gtgagggggt catctagtgg aacattaata attggactgc accccgatga agttgatgat 9480

gatgatggtt tggaggattc tgatcagctg aaaacgttgg atgacaaagt actattattc 9540

cataacctga ggttcctaac tagcttggca atatatggtt gtcgaaatct tgcgactatt 9600

tcaattgaaa gtttaaggca actcgtttgt ttgaagagtt tggaattata cggctgccca 9660

aaacttttct cttcagatgt tccaccagag cttacatgtg aatatatgtc aggagcaaat 9720

cacagcgccc tcccatctct cgaatgtctc tatattgagg attgtggaat aacggggaag 9780

tggctgtctc tgatgttgca acatgtgcag gccctacagg aactgagttt agaggactgc 9840

cagcagataa caaggctatc gataggagag gaagaaaaca gtcaaccaaa tcttatgtca 9900

gctatggagg atccgtcatt aggatatcca gatcgagacg aacttctgcg ccttccgtta 9960

aatctcatct cttctctgaa aaaggtatct attacatatt gctatgattt aacattctac 10020

ggcagcaagg tagatttcgc tggatttacc tcccttgagg agttagtgat ttcacgatgc 10080

cccaagctgg tgtcgttctt ggcgcataac gacggaaatg atgaacagtc gaatggaaga 10140

tggctcctac cgctatcact tggaaaactt gagattaact atgttgattc cctaaaaacg 10200

ctgcagctct gctttccggg gaacctcacc cgcctgaaaa aactagtagt gttgggaaac 10260

caaagtttaa catctctgca gctccattcc tgcacagcac tccaagagtt gataattcga 10320

agctgtgagt cgcttaattc tctggaaggc ttgcaattgc tcggcaatct caggttgctg 10380

tgtgcacaca gatgcctcag cggccatgaa gaagatggaa tgtgtatcct tccgcaatca 10440

cttgaggaaa tttacatctg cgagtactct caagagaggc tgcagctctg ctttccagga 10500

agcctcaccc gcctgaaaaa actagtagtg ttgggaaacc aaagtttaac atctctgcag 10560

ctccattcct gcacagcact ccaagagttg ataattcaaa gctgtgagtc gcttaattct 10620

ctggaaggct tgcaatggct cggcaacctc aggttgctgc aggcacacag atgcctcagt 10680

ggttatggag aaaatggaag gtgtatcctt ccacaatcac ttgaggaact ttacatcaga 10740

gagtattctc aagaaacgct gcagccctgc tttccaggga acctcaccag cttgaaaaaa 10800

ctagaagtac agggaagcca aaagttaata tctctgcagc tgtattcctg cacagcactc 10860

caagagttga tgattgaaag ttgtgtgtcg cttaattctc tggaaggcct gcaatggctc 10920

gtcaacctca ggttgctgcg ggcacacaga tgcctcagtg gttatggaga aaatggaagg 10980

tgtatccttc cacaatcact tgagggactt tacatcagag agtattctca agaaattcta 11040

cagccctgct tccagacgaa tctcacttgc ttaaaaagat tagaggtatc aggcactgga 11100

agtttcaaat ctctggagtt gcaatcatgc actgcactcg aacatttgaa gattgaaggt 11160

tgttcatcac ttgccacatt agagggcttg cgattcctcc acaccctcag gcatttgaaa 11220

gtacacagat gtcccagatt gcctccatat tttgagagtt tgtcaggaca gggctatgag 11280

ctatgcccac gactggaaag gctcgagatc aattatccct caatccttac cacgtcgttt 11340

tgcaagaacc tcacctctct acaataccta gagctttgca atcacggatt ggaaatggaa 11400

agactaacgg acgaggaaga gagagcgctt caactcctca cttccctgca agagctccga 11460

tttaactgtt gctacaatct cgtagatctt cccacagggc tccacaacct tccctccctc 11520

aagaggttgg agatctggaa ttgcgggagc atcgcgaggc cgctggaaaa gggtctccca 11580

ccttcgttgg aagaactggc tatcgtagat tgcagtaatg agctagctca gcagtgcaga 11640

ttgctagcaa gcaagcggaa ggtcaaaatt aatcagagat atgtgaattg attactcggt 11700

ggctttttcc acctgcccaa ctggcatggg ctcgttcagg cgttcaagct gctgtaaatt 11760

ccattgccgc aatgacgacc ttcagaaccg ttacacaata caaaggacat atgatggcta 11820

gatcaactgt cacatacaaa ttctataatt ctatctactg aaaaggatga ttgctgttca 11880

ttttcgtgat tacagaagga actgtgtata tcgtgctatt tttgcattca cattgtgttc 11940

caggttgtgc tcggatcagc caatttcggt ggttaattca ctttgctgtg tcctctgtgc 12000

agtgtgcact caagaattgt tcgcacatgc aattcagttg agcatcgcac tacgcaagtt 12060

tttttttttt tgttaacaaa ggagtggttg acagcattgc aggttgctaa aacagtgtca 12120

aaaatttgct aacaaaagaa ttctcttcag aaatagtatg aaaataaatg ccacataagt 12180

aatctgagta aaacagacag aatcactaga gaagactaac gacaaactct tctttttcta 12240

tttctgtggt gaaaaactca gcatataatc tcatgtatgc attggaggtg cataccattt 12300

cgatcatgta tgcatttagc tgaacattat caagaagcaa atttattgca cgggcaacca 12360

taagctaaac taattctcaa gcaatttaat aatcacaaaa cagtctgcaa taccaagata 12420

tataaaactt ttgccagcat ggagcaacaa cactgaaaca ctacagtcaa gacagctccc 12480

agaaaagatt atatgttgct acttcaattg ccacagttac aatcacccat aatgacacat 12540

caaattacat aagagtatta agagtatatg acagttgtaa caaaacaact gcattgagaa 12600

ctagaagaac agacagccac atgaaccata ttcacttctt cagatcagaa agcctggtga 12660

actaccagtc taccaattgc taccgtagtc tccatttttc acttatatgt acataggaca 12720

cctgcgctat cagaatagcc acaaagccag ttcactcgct acaaattaat cttgactaaa 12780

tccaaacaga taccaactcc tcttaatcaa cttacaaaac acctacactt ttgttgtctc 12840

tacaccacca atcacacaaa aggaaattaa tcatccacct actcgtatcc tactgttaga 12900

atggaggcct tacaccagag aatgggcatt gccagattct gagacactgt cctttgagct 12960

ccctgaatct gaaccagtaa caaaaatcaa ataagacaca acccaaaacc ttccgttcat 13020

caagaagttg gatgttcaaa ttataaagtg acagttcgat cgtaccactg gatgatgagg 13080

acccactgct cgcggcctca gtatccttct caatctccac tgattggtaa gttgctgttg 13140

gcatttcatc cccgacatct acacaggggc ggacccaggg ctggtgccgg gtatgcaccg 13200

gcatacccag aaaattcgga aaagatttag taggcagtat gcatcagtgg gctgaaacta 13260

agcaaaagcc caagaagtaa aagtctagct gttgcacagc ccaccagaag atgatgttcc 13320

cctttggcta gcactcatgc ggtagggttt ggagcaggcg ttggcggcgc tgcgacagcg 13380

tggcgcgctg tggcactgta tcattgatcg gcagcctcat ggcaagcacg acgcatggac 13440

ggtttgagcg tcgtggcgct gcggccggac agcgagcacc ggtggcagag gctgccggtc 13500

actgggctag gcgctgacta aatcacctgg tccagttcca ctcattatct gtccatgtcc 13560

aagtatactc aaacttcttc agttctttgt ctctctgatt tttttttctt aacagatgga 13620

gacatagatt gattgaaaat tgaaaactat aatgagctaa caagtgaaat caatgagagt 13680

acaaacttca aaaattgaat acatgttttt gtgatttgta cgctgagatc aagttgaatc 13740

aatcagatta gaatacgaag aagaatatgg gtattctttt tctttcttta gcaaagcacc 13800

attagattct gcttcatcag gccatgccct atgcttcaca tttctaatgg tgatgggaaa 13860

ccttagaaca gggagggaaa gtaactgaac aaagtgagtt gtgttatttt gttcttcaat 13920

tcaccctcag gatacaccaa tactttccta aaattaagac tatatttgtg tcatcatgtg 13980

catatagtgt ttgatttgcc tctcttgata gctagcatgg gcaaacccag gacaaaaatt 14040

ctgggtccgc cactgcatct acatactcat ccaactgctc ggcctgtgta gtgatcttgt 14100

caggatcctt gctctcctgt aaacaacaaa acacatctat tatacaatta aaggaataga 14160

aaaaggagcc tccacgttcg ctctcatggc ctagaaattc tcacattaat cgaagaaaaa 14220

gaaaaacaga gtccatatat agaaatacaa tttagaaata gttgaaattc gaaattaaaa 14280

aataaggaat attagaagat gagactagag tccatataga aatacacttt agaaatagtt 14340

gaaattcgga attaaaaaat aaggaatatt agaagaggag tatagagtcc atatagaaat 14400

acaattagga aataatagaa attcggaatt aaaaataagg aatattagaa gtagagtata 14460

gagtccatat agaaatacaa ttaagaaaaa aaatagaaat tcggaattaa aaaataagaa 14520

atattagaag tagagtatag agtccatata ggaatttaaa actaactaaa attcggaaaa 14580

aaaaaagaag ccaccacgtt cgctctcatg acctagaaat tctcacatta atcggagaaa 14640

aagaaaaggc agagtctata tagaaataca attcagaaat agctgaaatt cagaattaaa 14700

aaaataagga atattaaaag aggagactag agtctatata gaaatacaat ttagaaatag 14760

ctaaaattcg gaattaaaaa ataaggaata ttagaagagg agactagagt ccatatagaa 14820

atatgattca gaaatagctg aaattcggaa ttaaaaaata aggaatatta gaacaggaga 14880

ctagagtcca tatagaaata caattaggaa ataacaaaaa ttcggaatta aaaataagga 14940

atattagaag tagagtatag agtccatata gaaatacaat taagaaataa ccgaaattcg 15000

gaattaaaaa taagaaatat tagaagtaga gtatagagtc catatagaaa tacaattaag 15060

aaaaaaaata gaaatttgaa attaaaaaat aaggaatact ataagtagag tatagagttc 15120

atattggaat ttaaaaccaa ctaaaattcg gaataaaaag tagcctccac gttcgctctc 15180

atggtctaga aattctcaca ttaatcagaa aaaaagaaaa agcagagtcc atatagaaat 15240

acaatttaga aatagctgaa attcagaatt aaaaaataag gaatattaga agaggagact 15300

agagtagtta ttatacatta gtagttttga aaagttattg caaaatttaa aattatgttg 15360

tcattgtaat atattttaat aatataatga gaaaatatat atgatattat ataagagaaa 15420

atataatgat gctagccgcg taatctacgc ggaccaccat gctagttaac cattataaga 15480

gctcatttca cacataagct aatagcaacc aagaacaaag catgctgctc actattgcca 15540

tcgtcacatc accgttgtcc accatcgtag cggtactgcc attcaccagc atatcactat 15600

cattagccac ggaagcatca atgacatcgg cattttcacc attaacaatg gcagcccgtc 15660

gactcttgtt gagcgccttc ttgaatttgt tgacgaatcg atcaagctcc cactgtgtct 15720

cgacatccat ctcatcaata tccagctcga tctcaccccc aaccagctca ggattgccat 15780

tcctcttccg cacaatctgc aacacattat gcatcttctc ctctggcaag ctctccaacc 15840

caaccctcaa caagttcttc tcctccaggg tcatctccct cttgttcggc tcccttgcct 15900

tcgttttccg cattttcaca ttccctgccc ttggtttcac ctgcgccgga gctttcgccg 15960

gtggcaattc cggtggcggc actggcatcg gcggctcaag gatcttgagt tcctgctcga 16020

accaagacac cgacgctttg tacatcttct caaaggacgc gaggaggtcg ccggcgaagg 16080

tgtggacctc gtgtccagca gggttgtacc gcagcgcgtt gctgaacgtg agccggatgt 16140

cggcggcgaa gtcgtcgtgc gaggggtacc ttccggcggc gaggttcgcc ctcaccgtgc 16200

cgagatccat ggggcacttg atgacggcgt ggtagtcttg gagtccgagg cggtcgacct 16260

ccacgggggc gttgaaccag atgctccgct tgtccttccg cagcttcgcc aggatctggt 16320

cgcaccgctt ccgcatcgcc gccctgagct tcgccggcgg cggggggagg ccccggcgag 16380

gcgagccatg ggcgcttcgg cggcttctgc tgttgctgac cccacgagcc aatgcgggag 16440

atgaggcgcg gaccaggccg aggcccaact cgcctgcgac tccgcgaggc ccatgagagt 16500

tcgtttgggc tatcagtcca ttacggacaa gaagcccgag ggcgcggccc cgaagagctc 16560

gttagatgct gcgaaaatgt aggctatacc acgcgttaac tagccgaggc cagacgtttg 16620

tacgccaagg taatgatttc gcagcatcta acgagctctt cggtcgcagt cataacttcg 16680

tatagcatac attgtcgcga gacgtcatca cgttacacct aaaaagctta catgtgagca 16740

aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 16800

ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 16860

acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 16920

ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 16980

tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 17040

tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 17100

gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 17160

agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 17220

tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 17280

agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 17340

tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 17400

acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 17460

tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 17520

agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 17580

tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 17640

acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agaaccacgc 17700

tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 17760

ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 17820

agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 17880

tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 17940

acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 18000

agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 18060

actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 18120

tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 18180

gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 18240

ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 18300

tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 18360

aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 18420

tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 18480

tgtatttaga aaaataaaca aataggggtt ccgcg 18515

<210> 17

<211> 16915

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 17

cacatttccc cgaaaagtgc cacctgacgt ctttcgcagc atctaacgag ctcttcgcag 60

tccattacgg acaagaagcc cgagggcgcg gcccacatcc ttccgtcttc cccgcctttc 120

cttccctgcc tcgtcgaccg cgttatcccg tctcaagtct caactcccaa cctgcgatgc 180

gccgtagaaa tttgatactc gtgtcactcg tcgtcttcct ttgcttcact aatcactcca 240

atgacgcgac acgcatctct ctcaaaaaga aaaggattgg tatgtaactg tctacaagtg 300

atgaattcat cactcacgca gttcactgca catcgtccca gtacgcgcac tatcttggca 360

gcaaaaagtt gtaccagaat gatcttgcgt tcttggcagg acatgatcga tcaaaataat 420

ctcaagcact ttggctacac acaatgaacg aaattttgca gctcggcagc tcaccaagga 480

tgggcgcaac tcaaccacga ctgaaatcgg tgctgacact tttagcagca tctttgtgat 540

tcagccgtgg tttctccggc gcgtctcctt ctgttcagag aaaaccgaat agttcgggtt 600

cggattcagt tcgaagcaag catggcttaa aatcagttca tgcacacaca gctgccccct 660

tggcatttgt gtgcactgac acgatgccgt ctgtcctacg cgggttggta gtatgatgaa 720

agggtttgga aaccgatcta aaaaaccgtt cggatcatcg ggtttttgac tagccatgaa 780

agtgaaattg tccgaaaagg ataaaagagc gaacgacgtc gtcggtccag cacaaagctc 840

agcgctcaat cacgtcagag aaaccgatca cttcgggtcc gggttcggtt ctggagagct 900

aactaaggac ttacggagtg gtatgttcaa ctgttcagag tatttaactt cacggccaat 960

acgttgtcgt ccaccagagt gcatgtgagc gtcacgacgc atcggataat ttgaccattc 1020

gtaggttgct gttggcgagc acggcgaaac ccgagacgcg ggttcggttt cggttaatcc 1080

gtttcgcggt cgcgcgcggc cacgggcaca cggccatggc gccatgccgc tgtctccccc 1140

tcgctggctg cgacgcttga ctatttttat tcttcatctc catttgattg atttaatctc 1200

tcgttgcagc agcaggcaac gtgcaatcgg ttcggttttg ctactgttcg ggttcccctg 1260

gccctctccc ccctataaat acacgcctcc aaagtccaat ccaatccgcc atgaacactc 1320

ggtgctcgag ccatttccaa ttccaagctt acatcgaatc accatccacg acaaagaaga 1380

cgcatacgct ggtgatggag ctcatggcct ccctcatcaa catcttggcg ctgatctccg 1440

aggcatgccg cagcgcggag aagctgccgg cggcgctgat cactggcggc gtcgtggagg 1500

ccgcggcggc gatcttcgtc gccttcttca agccgcccgg tggcgtattt cagcaccacg 1560

gcaaggcacc attctacctg tattacggca ttataggagg cgtggccatc ttcggcttcg 1620

cggaggcgtg ggccgggttc tgggtctccg gcgacctgaa cggccggcgc gccgtcggaa 1680

agacgatact gtgggtgtcg attttgcctc ttgtcttggt agctgcgctc ggagggttcg 1740

tcttcatgag atagttgcaa acctatgtta atttcctctg tttgtacgag tgggtgtaat 1800

ctcctgtacc tctgtgtgta gtagttgtag cgtgtatgca ctagtttttg ctttgtaatt 1860

tttagcctgg tgtatttatg atatttcagt tattatgtga tgtaacagta tgattgttaa 1920

cttttacaag gttttatgta attatacata aggttatttt ttgtgtgata ctccctccgt 1980

tttttaatag tgacgccgtt gactttttct cacatgttta accattcgtc ttattcaaaa 2040

aatttatgta attataattt attttgttat gagttatttt atcactcata gtacttcaag 2100

tatgatttat atcatataca tttgcataaa atttttgaat aagacgaatc atcaaacatg 2160

tgagaaaaag ccaatggcgt catatattaa aaaacggagg tagtatgtga ttatgcgcgt 2220

tttccttgca tttctttagc tacggatgtt gtacaggtgt acctctttta tgagtattcc 2280

tccaatcttc tgcttatatc cttctcgaaa tcatggcagt aatcaaccaa tcataatctt 2340

aaaagaatca tggtagacgc agagcaacca gttagtacat atttgaaaga acaggccctt 2400

gcattgcagg gccgtatcct gtaggctgcg gtgtttcaaa gcaggaaaaa gtacaccgaa 2460

ggtccctcaa cttgttatcg agttacaaaa tcgtccctca accgcaaaac cataccggac 2520

gtccctcaac taacaaaact gttcacttta acggtggttt tgaccccggt tttatttgag 2580

gtggcggctg agtcagcgtg ggacccacgt gggccccaca tgtcagaatg ccaggtcgtc 2640

tctctctcct cttctctccc ttcctcgtct ctccctctca cttctctcct ctctgcaggg 2700

cggctggcgg gtggggagga ggtccggcag ccagcggcgg cgcgtgcgac ctcggggtcg 2760

gaccgtcgga gaggcccggg aggcggctgc ggcggcggcg gcgacgcttg agaagggaga 2820

ggcggccggt ggcgccggcg cgggaagaag ctgtcttcgg cgtcgtgccc gctctgccct 2880

ccaccgcctg cttcgcccgc cgctgccccg cgctcacaca ctcccgccgg cgacgccgct 2940

gacggcgcgg gcgcgagaac tgcggccatg gatgacgacg gcggcgacat cctcgctccc 3000

cccgttccgc tccccgtcgg cgccatcttc ctctcccgca gcgcgcctgc ccgccgctcg 3060

tctgctccgt cggccggccg ctccccgcgc agtcggcggt ggcgaaggtg accgtgccgg 3120

cgtcgaggtc gtagccgacg tggatgttct gctgcgccag gttcccaagg atggacaccg 3180

gctgctgctt cgtcgtcgcc acgattgcca ggcacagcgt cccctcctac accgtcacgg 3240

acgcgttctc cggcttcagc gccaccgccg cgcggccgcc gccaccgccg aactccagcg 3300

tcaagtccgg actcgcttgt tcggtttgct tcctgctgga tgtacacatg aggtcattca 3360

agatcagtaa ctcatactct attctagcaa gctggctgtt ggtgagaatt ctgaattctc 3420

attgcatcgc ctaacgaaga tagaagatcg gagtgttata catcaatcaa tttcttagct 3480

gggaatgtta ctcctgcatt gctctacaat ttatgggtca tgtaatttga tctctcacag 3540

aatctatagc gaggtaagaa agcgaaaccg gcgccacaaa atatcttgaa attctgcttg 3600

ttcatacact gttcttctga ttaaagaaca acttagcttg agattgttct gttcacttgc 3660

aatttgcagg agtaacaaag cttatttttg cattgcccga gtccacaatg atgcgggagc 3720

tcgccgccga tgccaccgtc ttgttgccga ccctgacgaa gtcgaggacc acggtgtagt 3780

acgtgtccac gtccccggcg acgagcggcg tgctcgctgc gccgagctcg gtgacgttgg 3840

cgagggtgcc gaagttgaac gccgacgatg agttgacgga gtgcgggacg aggctgtagg 3900

agaatctccg gccgagcgac gcctcgccgc cgagctgcgt cacgagggag accgcgtcgc 3960

cgctgagccc gaccagcccg tcggccggga acgagccggc cgtcgtggtg gagcagccga 4020

acttgacgct gcacacgccg cctcgtggtc gtgggcctcg tgggtggcgg ccggcggggg 4080

agcccgcgag ccgctcctac ccctgccgcc ctccaccact gcccgcccgc aggtcctccc 4140

cccactgctg ccgcgtcgcc cgtcgccgcc accaccgcag ccgcgtcgcc atcgaatcgg 4200

ggagaagtca gaaagacatg aggaaaggag agaagagggg aggagagaga tgacctggca 4260

tcctgatatg tggggcccac gtgggtccca cgctgactca accgtcacgt cacacaaaac 4320

caggatcaaa accaccggag aatctaaagt gaacggtttt attagctgag ggacgtccgg 4380

tatctggttt tgtggttaag ggacgatttt gtaactcgat gataagttga gggaccttcg 4440

gtgtactttt tcctttcaaa gcataatgaa gaaatcaaac ttgtcaggtc tcagatggct 4500

cgcaagcagt tctccggaaa aggaggagag aagaagatat ggacatacgg ggttaatact 4560

aatgcattag cataagctgt tcagttccgg gttcggttgt gacatgtact gatttctttg 4620

ttaatagtta atcagcggaa atcatgtctt tttcttattg gtgggtcccc gactgtggat 4680

ctttttgcgc gcctctcttg gtttggattg caaagaagcc taccgacccg gtttcgggtt 4740

cgggttcggg ttcgttgctc agcgcttgtg ttgctttgct caggcatact gcatacatat 4800

gctttcagaa tgtctctatc aatccgggtt gaaaaaagtg gtcttgttga gatgaaattg 4860

cttatcggca ccattcgggt tcggttcaag tcgtcggttt cctgtactac tgctagtcag 4920

aaaaccgagc tacgtggccg atcgggttcg ggtttccgag agcaaacaac gtagcgtgtg 4980

ctcgtcattt tgcaatcgct ccacttgctg cgagctcggc gaacggttaa tctggacgcc 5040

atccctacga aaacccgaga cgcggtctgg cgggggtcgg tgcttcggtt cggttcatgg 5100

tatagatggc ctttcggccc gcccggcacg gcccggccca ggaacggccc accagccatc 5160

gggccggcac ggcccgatcg gcacttcgtg ccgggccgtg ccgacccatg ggctgcacct 5220

cccgcccagg cacggcccac cagccgtcgg gccgtgccgg gccggcccga aggcgcgggt 5280

ggcccatcgt gcctttttat ataagtctat attccatccc tcctctttgg gccgtgaaat 5340

atatatagcg aaaataagtc tatttgccgt ccctcttctt tgggctgtgg catatatata 5400

gtgaaaataa gtctattttc tgtccctcct ctttgggttg acatatatat atatatagct 5460

aaaataagac tattttacgt ctctattctt tcgaccatgg catataatat gtctattttt 5520

actccctatt gtttatgtgt cgggcctagc ctgaggccca tcgtgccgtg ccggcccggc 5580

acggccgggc cggcccggta gtcgggccgg gccagcacgg gcccaacccc taacgggccg 5640

tgccgtgctt gggccgggcc aaaacgccgt gccgtgggcc gggccatcga gcgtcgggcc 5700

ttttggccat ctatagttca tggtgtgtgg gctgtgttcc ctatgggacc attcgggtga 5760

tttaattagc acgaaaaacg gagtagttaa ttagcacgtt gattaattaa gtattattta 5820

attttctttt taaaaatgga tcaatataaa ttttttaaag aactttcata cagaaatttt 5880

tttaaataaa catactgttt gtcagtttaa aaaacgtaca cgcggaaaac gagaaaggag 5940

tgttaagaat ttgctcttgc aaacacagcc atggatggac acgatggatc atggatcgct 6000

gcgacttgcc cgcgaatgaa tctccgtcga gggctcgagg cagtcctggg tttggctttg 6060

tgcgtcgggt ttcgccggtg tattaaacag gcacaagcat aaaactccaa gtcaccgagc 6120

agctccgatt tttttatttt tagtttttat taaaatattt acaaaactaa ttttgtgttt 6180

taaaaattta caaaactagt cacccatcgc ccgtggcaga tggcagttgc ctcatgccta 6240

acgggcggca aagaagggcg tgccattttg cagatggccc cccttgctgc ccttaggtta 6300

taaataccaa agaggccgct tccaaaatgc caagccgtcc tcgccgccct tttgcaggcg 6360

gcccccgcac agtagaaaat cgccctctat aagggtggca agggggccgc ctgcaaaaat 6420

gtccctacgg acggcaggag ggtagcctgc aaaatggcag gccttccttt gccggccgtt 6480

agacatgagg caacgatcgt ctgccacgtc attaaaatcg atctttagga gggatttatg 6540

gaccagtaag tccaacgtgt gcaaagtatt ccacccgaaa aatccgtcgg gaaaccgata 6600

gagtatatgc gtggccgttc gggtttatca ttttgtattt cttccgcttg cgcgcgttgg 6660

cactgcactg ctcactacgc tctcggcgag tgattactct ggtcgcgcga caacccgtaa 6720

cgcgatctcg cggtcgcggg ttcggtttcg gttgagctgt ggtgtggatc gctgcaactt 6780

ttgcttgaaa aatgaaaaac atgttcgtcc gcgattttct gtccgtcgcc tctcactgat 6840

cgagagatgc aacacagtct gagcgatcgg gttcggtttt tgtgctctgc tttcaccatg 6900

aagtcatcac caggctcact gcgtctgcct cctcgcacca ttcagcattc accgagttga 6960

aaaaaccaca aactaaggcg ttccccctga tgatacagca cgcgagctac gcacgcacag 7020

acatacggta cagacatgcc ggcgcccgca tcgacgtcga gaagctgctc actcatcccg 7080

tgccggcgtc ttgcaagtcg cggcggcggc gctctgcctg ggcttacggc ccaaattcgg 7140

cctgcaggcc cgattttgag ttgtcaagtc agagcccacc gacttggagg cagtcggccc 7200

aagcatttga aagcccagaa acatctgctc cgagtgctca ccttccgtgt agcttcctag 7260

tagttcctac actaccaaac taccgcatta tttatatccc tcacaaaaaa aaaataaaaa 7320

ggactacccg cattttatat tgctcatctg ccctcctcga atacctccca tattattccc 7380

atctctgcct tttgcttacc actgaccact gtcttctcgc gaggctcgcc actggtccat 7440

tgtcgcccaa gctccaaagg cttctcttcc gccggatctg atggaggagg tggaggtcgg 7500

tttgctggag ggagggatcg ggtggctggt gcagaccatc ctggagaacc tggacaccga 7560

taagctgggt gagtggattc gtcaggttgg gctcaccgat gacaccgaga agctcaggtc 7620

agagatcgag agggtggagg tggtgacggc tgccgtgaag gggagggcga tcgggaacag 7680

gtcgcttgcc cgatcgctca gccgtctcag ggagctgctc tacgacgccg acgacgcgat 7740

cgacgagctc gactactaca ggctccaaca gcaggttcaa ggaggtaaag cttgtgtatg 7800

caagatgtat ccatttttgt gtccaaggag gtgcattcat tccccttttt ctgttgtttc 7860

agatgcatgg caaggtggca ctggaagttt agatgaacct gaagcagagc aagcagagag 7920

accgagtatc aacgctgcta ttgcgattag cagtggtagc aaaaagcggt ccaaggcatg 7980

ggggcacttt gatatcactg aagaagaaaa tggaaagcct gtgaaggcaa ggtgtattca 8040

ctgtcacacg gtggtcaagt gcggttctga aaaagggaca tcagttttgc ataatcacct 8100

caagagtggc agctgtaaca agaagcgtga ggcaactgat cagcagccaa acccgtcatc 8160

aaggtacggt atatgattta tgtaacgtac tttcccccgc agcaagccag caactgtgaa 8220

accgttctgt ttttttttaa aaaatatttt tattttgcag tactgctgat actgcagcaa 8280

atagcactct tgttgaactc ggcggttcag gttcagacat cagaaaaaag atgaggatta 8340

atggtgagtc aacacacaac gatgcacctt atgcacaccc ttggaaaaag gctgaatgtt 8400

ccacaaggat acagcaaata actcgtgagt tacaagatgc acggggggct gtgagtgaaa 8460

ttcttaagct acatggaccg tgctctgttg gaaattcaaa ccatcgtacg agtacaacca 8520

caactctctg cagaagaacg tcaagtctta atccacacaa aatatatgga agagacgcag 8580

agaagaacac catcatgaag attattacag atgacagtta tgacggagta actgtagtcc 8640

ctattgtggg catcggagga gttgggaaga cagctctcgc tcaacttgta tacaacgaac 8700

caacggtgaa acgtgacttt gagcggatat gggtttgggt gtctgataac tatgatgaat 8760

tgaggatcac aatggagatt ctagattttg tctctcaaga aagacacgaa gaatctccct 8820

gtagaaaaga aatacggaaa ggagtaagta gctttgcaaa gcttcaggag attttgaatg 8880

ggtatatgga catccagtcg aaaaagtttt tgcttgtttt agatgacgta tgggacagca 8940

tggatgatta cagatggaat attttgttgg atccattgaa atcaaatcat ccaaaaggta 9000

atatgatcct tgtgacaact agacttttgt ctctcgcaca gaggataggc acagtcaaac 9060

caatcgagtt aggtgctttg tcaaaagagg atttttggtt gtattttaaa acatgtacat 9120

ttggtgatga gaattacaaa gcacatccaa gtttgaacat cattgggcag aagatagctg 9180

acaagttaaa gggcaatcca ttagcagcaa aagcaacagc gctgctatta agagaaaaac 9240

ttactgttga tcattggagc aacattctga tgaacgaaga ttggaaatcc ctgcatttca 9300

gtagaggcat catgcctgct ttgaagctta gctatgatca gctgccttac catttacaac 9360

agtgtttgtt gtattgttcc atattcccta gtagttatcg ctttgtcagc aaggagttga 9420

tctgtatttg gatttctcaa ggctttgtgc attgcaactc ttcaagtaag agactggagg 9480

agatagggtg ggactaccta actgatttgg tgaactctgg cttctttcag aaagttgatc 9540

atacacacta tatcatgtgt ggccttatgc atgattttgc aaggatggtt tcaaggactg 9600

agtacgcaac tatagataat ctacagagca acaaaatact gccaactata cgtcatttgt 9660

caatactaaa caattctgca cactatgaag atcctagtaa cgacaaggtt gaaggaagaa 9720

ttagaaatgc agttaaagca atgaaacatt tgaggacttt ggtgctaatt gggaaacata 9780

gctctttatt cttccaatcc ttcaaagatg tagtccagaa gggacatcat ttacgtctgt 9840

tgcaaatctc tgaaacatgt acttatgttg accccttgct ttgcaatctg gtgaatccag 9900

cccatattcg ctatatgaag cttcacaaaa gagctttgcc tcaatctttc agcaagtttt 9960

accatcttca agtattagat gttggctcaa aatctgatct gattatacct aatggtgtgg 10020

atgatctagt tagtctgcag catcttgtag cagcagagaa agcatgctcg tccatcacta 10080

gcatcagcaa aatgacctct cttcaggaac tacataactt tggtgttcaa aattctagcg 10140

gctgggagat agcacaactc cagtccatga accagcttgt acagctcggt gtgtctcaac 10200

ttgaaaatgt cacaactaga gctgaggctt gtggtgcaaa actaagagac aaacagaact 10260

tagaaaagct gcgcctttcg tggactaatt tacataaatt gggtcatttg gggactaacg 10320

tgccatggga tgaacgtgaa aatgcaagag cagtgcttga gggtcttgaa ccacatacaa 10380

atcttaagca cctagagata tattcgtaca atggtgctac ccctccaaca tggcttgcca 10440

cttcacttac ctctttacag actctccgtc tagagtgttg tggacaatgg aaaatgattc 10500

catcactgga acgtcttccc tttcttaaaa agatgaagtt ggagagtatg cagaaaataa 10560

tagaaatgac agttccttcg ctggaggagc tgatgttaat tgacatgcca aatttggaga 10620

gatgctcctg cacttccatg agggacttaa actgcagttt aagggttctg aaggttaaaa 10680

agtgccctgt gctgaaggtc tttccattgt tcgaggactg ccaaaaattt gaaattgagc 10740

ggaaatcatg gttgtcccac cttagcaagc ttactatcca tgattgtcct catttgcatg 10800

tgcacaatcc tcttccacct tctactattg ttttggaatt atccatcgcc aaagtttcaa 10860

cacttccaac gttgaagggg tcatccaatg gaacattaac aatttggctt cccaatgatg 10920

atgatgttcc tgataagctg ataacgttgg atgataacat tatgtcgttc cataacctga 10980

gtttcctaac tggattggaa atatatggtt tccaaaatcc gacgtctatt tcattccatg 11040

gtttgaggca actcagatgt ttgaagactt taaaaatata cgactgccca aaacttctcc 11100

cttcaaatgt tccatcagag cttaccggtg aatatatgtc aggagaaaat cacagcgccc 11160

ttccatctct cgtacgtctc catattgaga agtgtggaat aatgaggaag tggctgtctc 11220

tgttgttgca acatgtgcag gccctacagg aactgagttt agataactgc aagcagataa 11280

cagggctatc gttaggacag gaagaaaaca atcaaccaaa tcttatgtca gctatggagg 11340

atccatcatt aggatatcca ggtgaagata aacttatgcg ccttccatta aatctcctct 11400

cctctctgaa aaaggtatct attacattgt gcaatgatat aacattctac ggcagcaagg 11460

aagatttcgc tggatttacc tcccttgagg agttagtgat ttcacgatgc ctcaagctgg 11520

tgtcgttctt ggcgcataac gacggaaatg atgaacagtc gaatggaaga tggctcctac 11580

cgctatcact tggaaaactt gagattaaac atgttgattc cctaaaaacg ctgcagctct 11640

gctttccagg aaacctcacc cgcctgaaaa cactagtagt gttgggaaac caaagtttaa 11700

catctctgca gctccattcc tgcacagcac tccaagagtt gataattcaa agatgtgaat 11760

cacttaattc tctggaaggt ttgcaattgc tcggcaatct cagggggctg ctggcacaca 11820

gatgcctcag cggccatgga gaagatggaa ggtgtatcct tccgcaatca cttgagaaac 11880

tttacatctg ggagtactct caagagaggc tgcagctctg ctttccagga aacctcaccc 11940

gccagaaaat actaggagtg ttgggaagcc aaagtttaac atctctgcag ctccattcct 12000

gcacagcact ccaagagttg atgattcgaa gctgtgaatc gcttaattct ctggaaggct 12060

tgcaatggct cggcaacctc agggtgctgc gggcacacag atgcctcagt ggttatggag 12120

aatatggaag gtgtaccctt ccgcaatcac ttgaggaact ttacatccat gagtattctc 12180

aagaaactct gcagccctgc ttttcaggga acctcactct cctgagaaaa ttacaagtaa 12240

aggggaactc aaatttagtg tctctgcagc tccattcttg cacatcactc caagagttga 12300

taattgaaag ctgtaagtca attaattcgc tggaaggctt gcaatcgctt ggcaacctca 12360

ggttgttgcg ggcattcaga tgcctcagtg gttatggaga atatggaagg tgtatccttc 12420

cgcaatcact tgaggaactt ttcatcagtg agtattctct agaaactctg cagccctgct 12480

tcctgacgaa tctcacctgc ttaaaacaat tagaggtatc aggcaccaca agtttaaaat 12540

ctctagaact gcaatcatgc actgcactcg aacatttgaa gattcaaggt tgtgcgtcgc 12600

ttgctacatt ggaggggttg caattcctcc acgccctcag gcatatggaa gtattcagat 12660

gccctggctt gcctccatat ttggggagtt cgtcagagca gggctatgag ctatgcccac 12720

gactggaaag gctcgacatc gatgacccct ctatccttac cacgtcgttc tgcaagcacc 12780

tcacctccct ccaacgccta gagcttaact atcgcggaag tgaagtggca agactaacgg 12840

atgagcaaga gagagcgctt cagctcctat tgtccctgca agagctccgg tttaagtctt 12900

gctacgatct cgtagatctt cctgcggggc tccacagcct tccctccctc aagaggttgg 12960

agatctggtg gtgcaggagc atcgcgaggc tgccagagat gggcctccca ccttcgttgg 13020

aagaactggt tatcgtagat tgcagtgacg agctagctca tcagtgcaga actctagcaa 13080

gcaagctgaa tgtcaaaatt aatggggaat atgtgaactg attactcggt ggcttgttag 13140

gcgcaccttt ttccacctgc ccaactggcg tgggctcgtt caggcgttca agctgctgta 13200

aattccattg ccgcaatgac gaccttcaga accgttacac aatacaaagg acatatgatg 13260

gctagatcaa ctgtcgcaga gctagctttg gttcacctga aaacataagg ccaaacgcgt 13320

ggttctttta atcagaagta tcaaaaattg gttttggttt ttaacgaaaa gatgttagaa 13380

attggtttta atgtgccaga ttctgtacca gaattctgta ttttgctccg taattctgtg 13440

ctacgactga tgttgtattt aactgatagc aatcgaccgg caaaaccaat ccgctccaat 13500

gcattgtcac atacaaattc tataattcta tctactgaaa aggatgattg ctgttcattt 13560

tcgtgattac agaaggaact gtgtatatcg tgctattttt gcattcacat tgtgtcccag 13620

gttgtgctcg gatcagccaa tttcggtggt taattcactt tgctgtgtcc tctgtgcact 13680

caagaattgt tcgcacatgc aattcagttg agcatcgcac tacgcaagtt ttttttttgt 13740

taacaaagga gtggttgaca gcattgcagg ttgctaaaac agtgtcaaaa atttgctaac 13800

aaaagaattc tcttcagaaa tagtatgaaa ataaatgcca cataagtaat ctgagtaaaa 13860

cagacataat cactagagaa gactaacgac aaactcttct ttttctattt ctgtggtgaa 13920

aaactcagca tataatctca tgtatgcatt ggaggtgcat accatttcga tcatgtatgc 13980

atttagctga acattatcaa gaagcaaatt tattgcacgg gcaaacataa gctaaactaa 14040

ttctcaagca atttaataat cacaaaacag tctgcaatac caagatatat aaaacttttg 14100

ccagcatgga gcaacagcac tgaaacacta caatcaagac agctcccaga aaagattata 14160

tgttgctact tcaattgcca cagttacaat cacccataat gacacatcaa attacgtaag 14220

agtattaaga gtatatgaca gttgtaacaa aacaactgca ttgagaacta gaagaacaga 14280

cagccacatg aaccatattc acttcttcag atcagaaagc ctggtgaact accagtctac 14340

caattgctac cgtagtctcc atttttcact tatatgtaca taggacacct gcgctatcag 14400

aatagccaca aagccagttc actcgctaca aattaatctt gactaaatcc aaacagatac 14460

caactcctct taatcaactt acaaaacacc tacacttttg gtgtctctac accaccaatc 14520

acacaaaagg aaattaatca tccacctact cgtatcctac tgttagaatg gaggccttac 14580

accagagaat gggcattgcc agattctgag acactgtcct ttgagctccc tgaatctgaa 14640

ccagtaacaa aaatcaaata agacacaacc caaaaccttc cgttcatcaa gaagttggat 14700

gttcaaatta taaagtgaca gttcgatcgt accactggat gatgaggacc cactgctcgc 14760

ggcctcagta tccttctcaa tctccactga ttggtaagtt gctgttggca tttcatcccc 14820

gatatctaca tactcatcca actgctcggc ctgtgtagtg atcttgtcag gatccttgct 14880

ctcctgtaaa caacaaaaca cattaaccat tataagagct catctcacac ataagctaat 14940

agcaaccaag cgagacgtca tcacgttaca cctaaaatgt aggctatacc acgcgttaac 15000

tagccgaggc cagacgtttg tacgccaagg taatgattta ggtgtaacgt gatgacgtct 15060

cggtcgcagt cataacttcg tatagcatac attgtcgcga agagctcgtt agatgctgcg 15120

aaaaagctta catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 15180

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 15240

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 15300

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 15360

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 15420

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 15480

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 15540

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 15600

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 15660

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 15720

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 15780

aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 15840

ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 15900

gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 15960

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 16020

tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 16080

tgataccgcg agaaccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 16140

gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 16200

gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 16260

ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 16320

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 16380

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 16440

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 16500

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 16560

cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 16620

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 16680

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 16740

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 16800

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 16860

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcg 16915

<210> 18

<211> 14727

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 18

cacatttccc cgaaaagtgc cacctgacgt ctttaggtgt aacgtgatga cgtctcgaca 60

cattaaccat tataagagct catctcacac ataagctaat agcaaccaag aacaaagcat 120

gctgctcact attgccattg tcacatcacc gttgtccacc atcgtagcgg tactgccatt 180

caccagcata tcactatcat tagccacgga agcatcaatg acatcggcat tttcaccatt 240

aacaatggca gcccgtcgac tcttgttgag cgccttcttg aagttgttga cgaatcgatc 300

aagctcccac tgtgtctcga catccatctc atcaatatcc agctcgatct cacccccaac 360

cagctcagga ttgccattcc tcttccgcac aatctgcagc acattatgca tcttctcctc 420

tggcaagctc tccaacccaa ccctcaacaa gttcttctcc tccagcgtca tatccctctt 480

gttcggctcc cttgccttcg gcttccgcat tttcacattc gctgcccttg gtttcacctg 540

caccggagct gtcgccggtg gcaattccgg tggcggcact ggcatcggcg gctcaaggag 600

cttgagttcc tgctcgaacc aagacataga tgccttgtac atcttctcaa aggacgccag 660

gaggtcgccg gcgaaggtgt ggacctcgtg ccccgcaggg ttgtatctca gcgcgttgct 720

gaacgtgagc cggacgtcgg cggcgaagtc gtcgtgtgag gggtaccttc cggcggcgag 780

gttcgccctc accgtgccga gatccatggg gcacttgatg actgcgtggt agtcgtggag 840

cccgaggcgg tcgacctcga cgggggcgtt gaaccagatg ctccgcttgt ccttccgcag 900

cttcgccagg atctgctcgc aacgcttccg catcgccgcc ctgagcttcg ccggcggcgt 960

ggggaggtcc cggcgaggcg agccatggcg cttcacctga ccctgctgcc acgtgtcgat 1020

ccgggcgatg agggcgcgga cctggccaag ctcgccggcg aggcggtccc gcagcgcgcg 1080

ggcctcgcgg tgagccattg cccccggccg cagcgccacg tagcttgacg gcggcggcct 1140

cgtggccgag ccgttggcgc gctgctggag agggtggttg ggatcggaat tgggggccac 1200

gggcgcgagc ggggcacggg tctcccccca ctggtggtgg tggtggtggt ggttaacccc 1260

gccccggccg gcgagcaggg cggaggccat caccgtcgcc gtcgccggcg agggtttcgc 1320

gtgctgctgc tgctgcgctg gcgagagtgg gtggatccgc acggggaaag gggagaagcc 1380

gaggagatat tttttttctc tctctgtggc gggtggatgt gcccgaggcg agatcctgac 1440

cgttgatgtc tattcggagg cagatcgaac ggtggagatc tgcgtagagt tgcccgcggg 1500

cagtggggag tggacggcta ggatgttggt aatggtgtga cgtggcaaga tcttgctgcg 1560

tgatcgttat gacgtgattc gtgaacatat aacggtagtg aaggtgcaac tgggttatta 1620

tttgagtgat tagtactagt ttaagtctta ctacgtgatt gtacatttgc acttagctta 1680

ttactccatc tgttctatat tactcgttgc tttgattttt tttcgtagtc aactttttaa 1740

aatatgatag aaaaatatag caacatttga aacacaaaat tagtttgatt aaatctaata 1800

ttgtatatat tttgatgata tgtttaattg gtgttgaaaa tgctactatt ttttctctaa 1860

acttagttaa atataaagaa atttgattat gaaaaagaaa taaaaaaact tataacataa 1920

aatggggtcg tattttttta cacacttagc ttattacatt gtgatacaac tgtatgcatg 1980

tgccaatgct cccgtgtctt gctttcgaat ctctctgtaa agcgtgcgca ccaaaagaaa 2040

gcagcattat atggagggag ccacgccatg tgcccctttg ttctgcaagc atggtttgaa 2100

ctggtctccc aacctcctga tcaaaaggcc tacatgtatg tagccatttt tcttgcaaca 2160

attatatata caaagtagag atcatagtaa aagtcatcga tatatctaac atatagatac 2220

ataatataaa acgattacca ttttattagc tagcaagtac atagctgcca tgagcaggga 2280

ggcattagct actacttcct tttctttctt tctttctttt ttttttttac gttggttagc 2340

tcaaaattat atatgggaaa ggacgtagta gtatgacacg tttcgttctt cgtttaaaac 2400

cacaacgaga gcatcactgt atatatatgc ggatcgaata tatttacgcg ccgatctcgg 2460

agcacgctgc tgcttcggac gccggagcgg cggcgaggtg gagccgcttc tcgccgtgga 2520

cgatggcggc ggaggcggcg gagctcccga cgacgacgac gacgcaccgc ttctccttgg 2580

cctcggcgcg gcgcaccagg accaacgagc tgcagctgct gccgaccatc acattgcgag 2640

gaggaggagg cggcgctggc gccacggcgg cggcggccgc ggcgacgcca cggagcagcg 2700

cgtagctcgc cgacagctcg cgcgccatga ctatggccgg cagcggcggc gcgtcgggct 2760

cctcctccgc tatcgtgtcc agcatcgccg ggaacccgcg gcggcggcgg ggcctgatga 2820

tcgacgacag cagcggcggc gcgacctcga cggagaaccg ggcgacgagc tgggccttcg 2880

ccattgtcgg cgtcagcgtc agcgtcaagc gtgcgctacg cacagcgatc ggcagctcaa 2940

gaatctgtgg ccatggaagc ttcgtgctaa gcttactgga aattaatcgt agtctgggtg 3000

ctcgtattta tagacccgta cactgtcaat ttggaattgc aatttaattt tgagccatgc 3060

aatttgcaag tggatggttc tctctggtgg gaaactgacg agtagataaa ttttggaaag 3120

aaaatactag aaatgagacg attttggagg cagggataaa tgagttgaaa cttgaaagtg 3180

atcaggcgac cagagacact atcaacgtca ctgtgataat attaatgtag tagtaatgcc 3240

tagagaagtg ttgctatact gtcccctgat aaatgaagaa ttgtaaatga tgaatattca 3300

gcagggagtt tttttgtcag aaaatacatt gaaccttcag gcgtctaaag acagcatgaa 3360

caaaaaaaat ttacatacga acctcaaatg aacaatacaa aattttcaga gctaagacag 3420

cgtgaacaaa aaaaaaattg agagtgagaa catatgcata aatcattcat atagcatcca 3480

acacgtactg atgcatcatc tctctcatta cgcgacatac agtacagtat acaatataaa 3540

tgttgttttg taagcattct aatgaagaaa aaaatctcgg tacgcatcgc caataagaat 3600

ttcagcaaaa gaaaaacgat atatttagta aagactactg tcaatttcat ccaacttttt 3660

gtagaagatt gtagcgaggt ttccccatgg aggacggcaa tcacatgagg cggataagat 3720

cggaggagtt cctttcatct ccttttagga aatttaactt ccaggggcgc taggctgtcc 3780

ctgtacaagc cgtcctctgc ttgctcagcc agcaacttgg cattcttcgt acagtaccat 3840

ggtgcacgca tgggttatga aaatgaaacg aaagtttttt taaaaaaaaa caaatatttt 3900

gaagccaata ttatagttat aagaagtatt tgttgccaag attgaagcta tgtaaaactg 3960

tgatgatcaa taggacaata ggtgtgagtg cacctgtgat attggtatag gcccatttgt 4020

caatgtgtgt tacaagatta ttcgacagta atagttttca tgtatgggtt gtcagttcca 4080

tttccatgtc catgtcgaaa tgaaatatct ggtaattgaa acatcattat caaatgttta 4140

tttttagaga tcaaattgca gtaatttgcc ggaacggagc tgatgatata tgatcacagg 4200

ttttttttct ttttggcatt tcatctctct tctcatccac atgaacccag tgagtaactg 4260

ttgctccaca atcagtgagc agtgaatgtc aatgccaaga acagtagtat atcaagattc 4320

agtgaacaac atcccgagag tacttacatt ttggatgtcg tttggtgcca attggcagca 4380

ttcagaataa tgtgcagaga atctcatgcc catcatctta cgtttcagaa cagtagatga 4440

aaattcgcaa gccacagaat gatgacatgg aaggccagta ggagcacagt aaattaaaat 4500

cagcacaatc cattgctccc cttctctgag cttggttaaa ggattggaga tcagtggtgc 4560

tgatctatcg gtcgaaatca gtgtagctgt tactggaagc ccttcaccct ctgaaacacg 4620

ctgtgagctt tgtgaccagg taggttcagc cgttcagggg atccaaattc caatggcatc 4680

aatcaacatc gcgctctacg tcaatttcga gccggtgctt gccctttgca ggcagaaata 4740

tacagttgaa caaatactgt actaccccta gcatgttcct ttgcaaacca ccactttctg 4800

aagtcatact tgtatcatat tcatatgcct aaccttggcg tatccctctt atgctgttat 4860

gcaaatagga tggcataagc ttcatggaga tgtaaccagg catgcaaatt ctaactcgac 4920

caagatgaca ggaggcggct gcagtatttg ggcaataaaa atggcagttt caagtttcaa 4980

ccatgttctg cagctgaggg ctttggtggt gttttctttt cctttaagta tctttaaaag 5040

ggatacaagt agtaaccacc agttaatgaa atcccagcaa ttatgtcaaa aaaaattgaa 5100

atcccagcaa aactaggctc ttttggctct agttcctacc gggcatgctc ccgtagaaaa 5160

caacattacg tcaaacttgg gggcaaagca ctcattcatt ttcaatatcg atcgtttaaa 5220

ttaattgttc gtcacacata caccatcttg ttcagtaatg atggctcaca ttgtccgtaa 5280

tccctgctta actacatatt gactccctca ttttatactc aacaatacac accagactcc 5340

cacactccat tttcacagtg actttattta gcgcaccgca agctgcacca gcacactcct 5400

catgccaagg agaagcaaat aagataattg ctccaatgct gccttcctcg agcaatcagg 5460

ggaatggctt agtgcgctcg ccgggtgcca agtgccaaat ggccttagaa gactataatt 5520

tttgtgcagc tcctggtctg ttcacagccg ggagatgagc agtgggactg agacagtggc 5580

ggtctgctcc cccattgcag ggtggcacat ctccagcatg tccagctccc tgaaccgctc 5640

aagaaccctc attcggcact cctcagctgc catcagacca gtggagaagg cgccatgcac 5700

cgtgccagtg tactggacac tggtggcttc tccagcaaag aataggttat cgactgggat 5760

ccgcagcttc tcatacaggt cacgaggttt gcccacccca tcgaaggtgt aggatccaag 5820

tgtattctcg tctgagcccc aatgtgacac taggtaatgt atctgcaata agatttgggc 5880

agcatggttg taagaactag cataaagaga ttcttgattg ccaaatttga tatttttgtc 5940

aagatactaa ccggctcggc agcgttgggc aggatcttct tcagctgaga gaaggcaaat 6000

tgggcagcag cctcatctga cagcttttca atgtcacatg caagccgacc cgcaggcatg 6060

taaactagaa caggatggcc agtagccttg tggaggttga ggaaatagct gcagccatat 6120

gtggtggatg aaactactcc aaggaactcc acattaggcc agaaaacctc gctgaagtgg 6180

agaattattt tgttctcgac tccaactgag agttctctta ttgcttcctc cttccactct 6240

ggcagcctag gttcaaattt aatggtgttt gctttaagaa cacctaaggg gacagcaatg 6300

actgcagcat ccgcaacaaa tgttttacca ctgcttacag taacctccac cctattcctg 6360

tggcgaacaa tctcaacaac cctaaagaaa agaaaataat gtggattagc aataaggaat 6420

ggtggatgga accatgaatt ttgcttttaa agtgagaagg aaaaactaaa tggatatgca 6480

tacctgtggc caaggcgtat atctaggcct tttgccagag tatttattac tggacgatat 6540

ccacgaacca tgagaccatg gccaccagga agcagtacct cctgcaccca cagcacagta 6600

cgaacatgag acatagcaat caaactagca tgagtacatc atgagcaaaa tattggcaat 6660

tgtagctgat atcaggaacc aaagtcctac ctggtcccaa ccctgaagag agattgcgtc 6720

cgcatcagta gcaaaccaac cttccatgcg gcacaaatac cactgaagaa catcatgagc 6780

aatcccttct tgcctgttgt gaacaaggac aggtcaggca aaatgaccaa aagatcagga 6840

taacctatgc acatgggcag aaattggatg gtacaaacct caagtgtgga tttctctcca 6900

taacaattgc aatggccttc gcaatagaaa tgtcttcctt agtttcttcc ctcagtttgc 6960

cagtctgcac atataaatgt tagggaaaaa caagtaccaa aaatattatc atgcaacaac 7020

aataagcatg gaaactgaaa caaggtaata cctcttccaa tatagtctca aaaaccttcc 7080

ctatcttttc taccagctct tggggaactt gatggccctt agtgtcatag agagcataac 7140

tgagggaaaa aacatgttaa atggttgatt attatggtcc atcatctctc aagttacata 7200

caaaataaac tagctctagt aacctctcaa ggtcatgatc aaacagcaca gagtcatctc 7260

cacttgtgcg atacagtgga agtccaagcc ttccaataat tggtgccagg ggattttcct 7320

cacaaacacc atgaagcctg atgctcacac aaaatggaag tggattaaaa ataatggttc 7380

cagataaact tttgccacca tttcaaataa ttcagaaaat attgtcagag aggaacctca 7440

ccaggatgct cccagatcaa caggaaagcc aaaagagtaa tcagtgtgaa ttctaccacc 7500

tatcctgtca cgagattcta gaagaacaac ctggaaagaa accacattag aataaccagt 7560

ggggaagaat ttcacaacat ttgaaatgca cttcaaggga tcattgaacc tatatcatta 7620

caattaggcc ataaactgca tcacatatta cctcaaatga tgcattcctg agagcgttgg 7680

ctgcagcaat gcctgcaaac ccactgccga taacaatagc agaaggtgtg tgggactttc 7740

tcctaacatt ttcaccatat gaacctggat tttacaaaaa aaaaaggaaa aaaaaaagga 7800

acctcaatga gagtgacaaa gtgtagtaat aaaaagttaa aacattataa aataataatg 7860

atgtgtcaat ttttggttat tatcttgcca aaatattgca ttgaaaaagg agctcattca 7920

gcttaaatct aaagtgcaaa tactggctgg cacatgtacc atcatatgtt ctttatatga 7980

ctgtcaacac aggaagcaac ctgaaagttt cacatgtttg atgtagttgg atggatgctt 8040

gtgtttcatg aatctaaagg ttttgcacct taaggctaac ggtttgcttc attgtgaaag 8100

ggggttttat acctccaaat gataaaaatc agtttataca gaattaacat aaagaagcct 8160

accaagtgat gaaattgccg atatcgcctg ttcttctcac atcaaccctc ctctaccccc 8220

tccatcatgg agggttagta gtgaggcatg ttcccctgtc tcagaacaat gatggtgagg 8280

taagacagga caattttaac attctgttga ttccccacag aaacctaacc gcttggtctg 8340

tagctacaca ttggaagctg agatgtagga ctgatcaaat ttttcgctgg gcaggcacac 8400

caaagactct ccgttaaaac acaatgacct caaaggatgt gcaaaaggca aggacacaat 8460

attattattg tactgttctt tctagttatg tggacatggt gcaaattcag actaatgggc 8520

gatagacatg acatgaattt gtggctatat actgtttatc ttggaattat ttggtggcat 8580

actataaatt agacaatctt ttagcgagac aaccctctgc cggatatgct tcagagcact 8640

taacaagcaa aaaataatgg ccaccgaaca tctcacacaa tgactatagg cgcactaaaa 8700

ggcacaatcc aaaagtaaag atatccataa atgagacagc aacgaaagcc aacagaaaaa 8760

acattttata agagctgaac agtaaaataa gaaaatggtg gcattggaag ataaagaaac 8820

ttagataact agatccacca gcgaatattc tcatataata atctgcaccc tacatgcatt 8880

acaatactat atgaatcatg catgcataga atgtccgcat ggcactaaac ttaacatgac 8940

atgcactttt gctgatctaa ttttagaata gtaagtatat gatatttaaa gttcaagcac 9000

tactgcatct gcatgttgtt agacctcaac tgcaaccagt atgtgagtgc ttgcatgaac 9060

ggtttacact agagtcttac aaattgcaag ctcttcagga actaaaaggc gcagcaggag 9120

cctacaactc cacatggagg actttctctc gccatgagaa aaaccctaga aggttccaat 9180

tggttgaggc gctggtacaa caggtgtaaa cagctaatgt tttgttttct cttcctacca 9240

ccgacagagg aatatgggaa ctcatccacg atggctgatg tctctttcat ctggcccact 9300

aggcacctcc caacaaccat aagcaactca gtgtgtttag attatcaaag caattttatc 9360

actacaacca cctagagagt ggaaaatttc ggtttttgca ctaaggttgc attaagtgca 9420

caaatcccta aaacaataca tgttcgatat tctactacta gcagaggtat tagcatacat 9480

cagcaaactt tataaaacag tttcttctaa attcgatttt ccacctaact ttacattaac 9540

agatgcaaaa aaaaaaagtc ttgaactgag agggggactt tataatggaa taaacaaaat 9600

aacataaaaa tcagcaacag aatcaccaca aaaacaatgg atgagaaaat ctgccaaacg 9660

aagccaggaa cttactgttg ttcgccattg ttggggttct gtttctctcc ttcacgatta 9720

ctactcgaat tcgaattaca attggatgga tttaacagaa actgcgggga gaaatgcagc 9780

aggaagatga tgaggcaggc cacgtagtgg ttcgagttcg agttgagtcc caggttcacg 9840

aaacagttca tgcagtcgtg cagccaaatc gccgcaacct cctagcaatt tgatgtccaa 9900

ccctttcacg atctcacgtc agcgccgaga attcgcgcga tcccatcaac ttctcaggca 9960

atccggttta agtctggatc aactcccgcg tgatatcact gcgaaaaaat caaaggaaac 10020

caaccgttta ttattcaccc aaatttataa accttcagtt ttagtcacac aaaaaccgct 10080

tcccagcaca cgaaatccgc cgcaaaatcg agagatttaa gcgattcaag aaagccgatg 10140

attaaaacta gaccaaaaat caacccccta agtcatcagg aagccaatct ccgaatccca 10200

caccagaagc gatcgaatcg agccaaggca atcaacaaac ctagggctcc ctatctttcc 10260

cctcgcaaac ccagtccaaa aatcgcacgc aagctatacg aaatcgcgcc acacacccac 10320

ctcgagattt cgattcgagg cgcacgcagt cgatcggagc aagagcaaaa aaaaacccga 10380

agcagtcttg cggcgatcga tcgatcgatg gatcggatgg ttttcgctgg atttttggag 10440

attttcggag agggggattt ggttttgggg agaggaggag aacgagacct tccgctttcc 10500

tggcgtttcg ggagcttttt gggcttttgg aggtgacggg atcctgtcga gttggggggg 10560

gggggggggg ggggatttat agcttccttt tcccgggggt tacgtgcacc accagggtca 10620

gcttaccggc tacgtggggg ccaccccacc aaacccgaca tatcttcacc gttgatctgg 10680

attggacggt ccggatcgac gccacgtcgc ccgaaacgtt ggtgaccgca catctgacga 10740

tattttcagc gtacatttac accctactat gaaatacaca accgtaaatc gtacataatc 10800

atcgtgtaca atagggtcta ctaagtaatt tttaagattg tacaccctcc gtttcacaat 10860

gtaagtcatt caacggaggg agtactctag tactgttttg tcttaaatta ttttttttct 10920

ttttatattt aattaaagta taaacggacg cacacgatac atacacttca ctttttactc 10980

actccaccac acacccacac acgcatgcac gtctttttat agccaggcta gaactcgtaa 11040

cagtattgta ttatgcaaaa actgaacaat ttcacgtgaa acatctgcaa atctcacggg 11100

aagccctcga gtttataaaa aagtttcata ccgaataata atgtaatgtc atctgtgctc 11160

atccaaggtc atttgtgtga tacaataaca cccaatggct ccactagatg gtactacaat 11220

tggaggagca caaactgcac agatgagaca tgtcctgcac agatgagaca tgtcattgga 11280

cgttggaatt taactaggta tatcttcctc caattgtccc atgattaatt ttaaaaatac 11340

ttaatacctt tcgtcttcca tggtgagttc attgatgatg caacacacaa tcagaggacg 11400

gtcgttttat tttcttgacc tggtaaaggt gcatttatag gtaacacgtt tttttaatcc 11460

tctccttaac tatacccctt ttgcatatga gagccacttg cacatttatc taacaaggag 11520

caactcgaaa aatcaaaatc aaaaggaact taattcacca ctctgactca ttccattgac 11580

acgtcaccag ttgcatttcc acagcttatc attgcatgca acacaaaaat tgggaggaaa 11640

attatcacaa ggaggaccat ctcttctccg ttctcacaaa ccatgcatta gcaccgtttc 11700

acagagaaga gatccccttc cgaaaaaaac aaactttagt ttatctgata tttcaaaaag 11760

ttatataata cgtagtacta actggcatgt atatcttgaa acccgtcgat ccaaaaagaa 11820

ttctcaaatc gtaggaagaa catattcact ggaaaggttg atatcgactg gagcaaacag 11880

catcacgatc acgactggag tacagtaaca agatggcaaa ttaatgattg tctaattgac 11940

gtactagtac agtttttttt tttatttttc gtgaacgcgt aaaaactgtt caagtcactg 12000

tggatatgtt tggaagatca taaaatttta aaaagctacc ggtaaggata tgaaaaggtt 12060

aagttcattt tctaaattat ataactatat attctcaaaa tttaaataaa aatctagacg 12120

gttgagaaag cttcgcaact gaaaaatttt aaaagaagct accggctacc ggccctaccg 12180

ctgtcagaag ttgtccaaac agacctatat agtttcgtgc ccgtttatca ttggaccgcc 12240

tatgaaccca ctgctagcct cgtgctcgta gattggcgac aactagctac gcatgcaaaa 12300

acgatttgag aaggaccgag aaagacgtgc gtgatggggc cagataccgt ggcctggcct 12360

ggcctaaccg gggccatcat ttcatccaac gcacagacca ttaggctctc gctatccgaa 12420

agaacagaaa attaaaacaa attcgccacc acaaacgatc gcgtcgctgc atctgctctc 12480

tatctgtaca cggagataag acacgaggga attatgcaca aaaaagtctc tctctctctc 12540

tctctctctc tctctctctc tctctcattt tcggaggacg acgttaatct cgttaattaa 12600

ttcagaacac gtaatattca gaatatcacc agttgatatt atcacataat ttagactttt 12660

aacagagacg tgatctacag tgcattattc agaggatgcg gcaaatatag agatgatcat 12720

tatatcgttg aaacggatga gcgcgcgtag ttaatctgtc aacgaagagc tcgttagatg 12780

ctgcgaaaat gtaggctata ccacgcgtta actagccgag gccagacgtt tgtacgccaa 12840

ggtaatgatt tcgcagcatc taacgagctc ttcggtcgca gtcataactt cgtatagcat 12900

acattgtcgc gagacgtcat cacgttacac ctaaaaagct tacatgtgag caaaaggcca 12960

gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 13020

ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 13080

ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 13140

gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 13200

ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 13260

cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 13320

cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 13380

gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 13440

aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 13500

tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 13560

gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 13620

tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 13680

gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 13740

tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 13800

ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 13860

ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagaaccac gctcaccggc 13920

tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 13980

aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 14040

gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 14100

gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 14160

ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 14220

gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 14280

gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 14340

gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 14400

tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 14460

gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 14520

agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 14580

aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 14640

ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 14700

gaaaaataaa caaatagggg ttccgcg 14727

<210> 19

<211> 53049

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 19

tagaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg 60

ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg 120

gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga 180

caacttaata acacattgcg gacgttttta atgttagact gaattaacgc cgaattaatt 240

cgggggatct ggattttagt actggatttt ggttttagga attagaaatt ttattgatag 300

aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga 360

aaccctatag gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa 420

ctcgagcttg tcgatcgaca gatccggtcg gcatctactc tatttctttg ccctcggacg 480

agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac 540

ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat 600

tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg 660

atagagttgg tcaagaccaa tgcggagcat atacgcccgg agtcgtggcg atcctgcaag 720

ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct 780

ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc 840

aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg 900

cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg 960

cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc 1020

agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa 1080

tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tagcctccgc 1140

gaccggttgt agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg 1200

tgcacggcgg gagatgcaat aggtcaggct ctcgctaaac tccccaatgt caagcacttc 1260

cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc 1320

atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc 1380

acgagattct tcgccctccg agagctgcat caggtcggag acactgtcga acttttcgat 1440

cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatctc attgcccccc 1500

cggatctgcg aaagctcgag agagatagat ttgtagagag agactggtga tttcagcgtg 1560

tcctctccaa atgaaatgaa cttccttata tagaggaagg tcttgcgaag gatagtggga 1620

ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt 1680

ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc 1740

actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta 1800

ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa 1860

tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc 1920

tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttatc 1980

acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct 2040

cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt 2100

tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat 2160

gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg 2220

aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac 2280

gagagtgtcg tgctccacca tgttggcaag ctgctctagc caatacgcaa accgcctgca 2340

ggtctagagc ttcccggggc gcttatctcg tgcggcgtcc tccaagccgc ggctgcactc 2400

tccctcgtct tcttcatcgc tccgagcgga gtcttcggca accatggtaa ggcactccac 2460

tggctgtact acggaagcct tgtcaccgtg gtgctcgtcg ggtttgtaga ggcttctgtc 2520

gggttttggg tggcaggtga tctggcaaac cggcgagcag tgggcaggac gatgctcttg 2580

gtttgcctct tcagcctgat cttcgtggtc actctttggg gctctgttct tgtgagatga 2640

ttgcattctt tgctgtatgt acaagattta gcattccttt gtgagtatgt ggtatttttc 2700

aactgtgttg tactgttgtc taaatatatt cattgtaata ttcaaactgt gtgagatttt 2760

catttgtagt ttcagaaatt cttggacgat ttttaccgta cttttgattt gggctgtttt 2820

cgtgtaatta gtactactag tatttacgtt gtcttttcag acgatcgcag ttctgacatg 2880

tgcttacgct gcctctattc tacttccgat agcgctgcac atatttgcac tcgcgttcag 2940

aaggcaaatg cagcaaacaa gttgtaatgg ggttcagcac ttcagcctct tctaaatata 3000

tccagcactg cagcatgctg aaacttctcc ccttccagtc caccaagatt tcaacttcaa 3060

attttgttca tacttaactc aaaatttcac gaactgatca ccctcgagtc gaaaattttc 3120

aatccacatg caaacttctg tagctttcac tagtctacac taatataaaa agcaccagtt 3180

catcaaaccc aaatattttc tgacctttag atctattcat ccaatggctc agatcaagag 3240

ttggatcaac ctctacaatt agcgattcca cgtcgtgtaa gtagcggtta caaaaattaa 3300

ttagcgatca tcaaacaaaa agaaaaaaga agaagttatt agtgatccat ctcccactcc 3360

cctaccctgc cgcctccgcc agtccgcctc cttgcgccta ccccaccgcc gtcgcccgcc 3420

gccttctcca ccagcgccga cgcctcctca cgcctacccc attgccgtcg ctgtcgtcgg 3480

ccccgccttc tccgccggcg gcagccgcga aacctacccc gccgccgacg tctcctcgcg 3540

cctaccaccc cctactctca cgctccgtcg ccgatctcct tcgccgttgc cccgccatcg 3600

atctccgacg ccggaactcc gttgcatgtc gcacgtcacc cagatcggcg tcgctccaag 3660

attggcgtca cggtcgcggg cgtcgatctc gctcccctat cggcatcgca atcactggcg 3720

atgatatcgt ggcctcgaac cttcgtagct gtctccatcg ccttcgccgc tgctgatcct 3780

gcttccccag ttcgccgtcg tcgccttcga tccggcgggc cacgcctctg tccacgccca 3840

ccgcggagtg cgcccctcca gccagtacag cagcatcgcc actgccgaca ggagcagcat 3900

ccccgtccag attgtcagca gacaaccagg tgattggatt ggttttcttt tatttggttg 3960

gattctgcat tttgaacgaa agcagtaaaa ttcatcattc tttgatttgg ttggattcag 4020

aattgacaat acatcataaa tctgagtaaa tcattaagca atattacatt ctctattatt 4080

taattattgc aattgttaat catgcttaaa aaacataatg ttattcatgc tcaaccataa 4140

tgctggcaac taaaaataat acctagattg acagtagtac agttgaacaa tacctagact 4200

gaccgtagta cagttgggtg gcgaaggtct cattgctcac tgatggcttt ctaaatcccc 4260

tattccgtcg taagcctgat tatcgtggta tattatttct tggaggtgtt ataggtgata 4320

tgaatggaag ggcatgtaca ctgttatagc aggaataaaa cctaggcttg atgctaattt 4380

acatatgaag aacacgcaaa ttaatttaat taattgttat gctttattct gcttgtttct 4440

atgcactaca attccaattt gagtgcctat cttgtcatgc tattcagtat aatgtcttgg 4500

caggatatga atctgttcat gcatctatat acagccttca tataattaag taaggattaa 4560

ccagattact tgctttgtct aatgcagcct caccatttca tttgtggcga gcaccttaat 4620

tccatcttgc cattgtcaag agtatatcat ctgcatctaa ttctgtatat tgagtatata 4680

ttgactagga tcttggaaat ttttgaattg ttgtcagata ttaagttcct tagtttgatg 4740

atttgattag ttttgcaact aatcaccatt gatcacaagt aaacaccact tatagcagtt 4800

taatggctat ttatctaaaa aggaaaaaac tagaagttta atgactgaaa ctgcagtggt 4860

actatgatct ataaacaaga aatctgctga gtttcctagc agttgttggg tgcactttcc 4920

tcttgctgtg tcatccatta aatttcaaaa actgcaaatt ctttgtatag gtattactgg 4980

gactcaaatg tacacctttc agttcacact caattttgta gaacagtaaa agcgtgaaaa 5040

tataggaaat ataattgatt tgttcagaaa attattcaaa tgctaacaca ctgcctaggt 5100

aatcactttc tcaacataag tatatgtaca acaatgcata ttgtttctac ttttcacaat 5160

tgggaacctg aatacataag tatatgtaca acaatgcata ttgtttctac ttttcacaat 5220

tgggaacctg aaatatcaag attctaaaac taatgtgtgt ctgctgtaca gttcttaaaa 5280

caatgctatg gaatattcta tgactaatat gttgcactgt agagttctga agattcttca 5340

atcaaagcgt gggaaacttc tgagggaaag tttgtcaaaa aaattcaggt taccagtgtt 5400

ttggtggaca tttacatact tatgcaattt gatatgctaa atgaatttac tactaataca 5460

ttacatcaat cttctgtttg taactggttc aatggaccat tagagctttc tgttgacaaa 5520

ttaaatgagc tttgttcatt tatccaacaa cttttctttt tctgaatctt gctccaaatt 5580

ttgtcatatt attgactatt atttttttta aaccatgcaa ataatttgtt gatataacga 5640

tgttcacttg gcataagaaa tcatagcagt ggaggtaatt ttttttctct ttccactctg 5700

gaaacccatg cccctttttt tttcctgatt cattacaagt gacattactg tgcaatcttg 5760

caactgtttc acagatgcaa cccttagtgc tttgaataat ataataagat aatgattccc 5820

ttttagctgt tctatgtgca gcaaatatta ttattggctc cgttcactat ttggtagatt 5880

gtttcccatt tcgttggaca accagaacaa caatcgattg tatgatttgt atcttagtcc 5940

tttgcaagta aggtattgag aaaatgacat aatgggaaca gaggaagtga tgtaattctg 6000

taccttattt gtgaacctta atgtatacat aatatttatt tcaggaagac gggactgaat 6060

acatatttct taattccaaa ggcattcagg atgtttatga aagataatta ctgttgtttt 6120

taggaaacat attacatcgg cgttgtcagg taagctaaca tagagtgatg atgttcatgg 6180

tttcaaatga gaatatatat attcctcgca tggacaactt tactatgctt tgtcaaggag 6240

tatgtaaggc aaacagtaac agggattttt gccaaactac caaactagga aagagataga 6300

ccttaccgaa aatattatgt ataaaggttt cttggaatat tagaaaaata taatgacacc 6360

gtagcgttag cacgggcata ttactagtag aacaaaagaa acacttatgc atttataccc 6420

aaaaaaaacc gtaattaatg gaccgcattt cgtactgtgt agcggcccgt cgaagcataa 6480

ctcaaacttt taagccagag atgggcctgg gccaacatcg acataaactg gaataagtcc 6540

acttgccttc cctcaacttt acatggagtg tgtatgatgg ccctaattcc caataccaga 6600

tgtcgacccc tcaagtcttc aaaaccatgc aaagtagttc ctcgagggta tagaccatat 6660

ttcccctggt ttccattgat gtggcatata tcagggccca ctacctgaac catcgccacg 6720

tccagtcctt tcggcctgtt cgcgatgacg agctcggcca ccacctcaac atcgtccatg 6780

ctgcggcgat ggcgaggaga cagctgggac ggtgaacctc atagctccgt ctgttgcaac 6840

atcctctcgc catgctcacg ccgccacgac acccttgtcc tcctgctccg tagcagcctc 6900

gcaagctcac gccgcggcct catcgaggtc ccctgtcccc gagccgcttc tagcatcgat 6960

gagctgagga gctcccccac tgccgaggcg atgacgattt ggcagggagg gctcgattcg 7020

cccactccaa ttcgcagcgt cacacctccc ctcccccctc ctattcatcc accaccaacg 7080

cggataggag aagggataga ggaaggtgtg cgtggacact tacatgttgg ttccatattt 7140

tttttttact cggatgccac gtcagataaa acaaccgtcg gtcaacattt ttcgtagtgg 7200

ggattaggga tgtcatacat gactcgacgc agagctgagg gatggcaagt gaacttattc 7260

ccataaactt ccaaaagttt ggaatgctca acttaggctt ctcttcggat gctgaagtgc 7320

taatgggcca aataaccaac tagcccacag cccatcgaag tggaagccga cgagtccacc 7380

gtcaacgcgg cagtagtaat ccgagaaaac gcgggtcgcg cgaagcaatc gatcatcatc 7440

agacgattaa tccacgacga ccaggagcgc tgccccgagc cacacgccca cacgcactgg 7500

tgaatcttct cctttccacc tagttttcac tctgcagtct cctctcctag ctactactct 7560

gccttcccat cctcgcttgt tgcagccctc ttgcacacgc cattggccac agtccaagga 7620

ctgctcctgt tccgatggag gaggtggaag ccggttggct ggagggcggg atcaggtggc 7680

tggcggagac catcctggat aacctggacg ccgacaagct ggatgaatgg attcgccaga 7740

ttaggctcgc cgctgacacc gagaagctac gggctgagat cgagaaggtg gatggggtgg 7800

tggctgccgt gaaggggagg gcgatcggga acaggtcgct ggcccgatcg ctcggccgtc 7860

tcagggggtt gctgtacgac gccgacgatg cggtcgacga gctcgactac ttcaggctcc 7920

agcagcaggt cgagggagga ggtactgtct ttgcatatat ccgtgccttt taattaagtt 7980

tgcaagctgc gttgcctgca acaatggcgt attggcgtca gtttccaatc catgcttgtg 8040

ctacagttac tacacggttt gaggctgaag agacggtcgg agatggagca gaggacgagg 8100

acgatattcc gatggacaat actgatgtac cggaggcagt ggcggcaggc agcagcaaga 8160

aacggtccaa ggcatgggaa cactttacta ccgtagagtt cactgctgac gggaaggatt 8220

ctaaagcacg gtgcaagtac tgccacaagg acctatgttg cacatctaag aacgggacat 8280

cagctttgcg caaccatctc aatgtttgca agaggaaacg tgtaacaagt actgaccaac 8340

cggtaaatcc atcaaggtaa tgctaatgga gttctgaatt tagtgtaaat ccgttgaagt 8400

gtaaatttgg cccgttacat ctgcttaaga tctcattctg tctctaatct tctaatagcc 8460

aactcatggt catttttttt cctaatatat agtaccggtg atggtgcacc aaatgtaatt 8520

agatgcaagg aaacaaaagt gaacaattgt atatatcaaa tataattata tctaaaacat 8580

gagtagtgta tcaaatccaa ttctttcaaa aatctactat gcaaaattga gtgacaaaat 8640

ctgctgcctt ttttttttta cagaaagcaa ccaattaata taagtcaaat ataaaaacgc 8700

tttgtagtct ccaataaaat agctcattgt ttcgtttata cttatgttta taaatttaaa 8760

tttaaaactt aattttggag ttgattttgt ggttttcttt tcatcctatt ttattttaca 8820

acatttgatt ttgaatagtt aagaatgcgt atataaaaat tttacccata agttattttt 8880

taaattgtta ataaatcgta aggataatca taagtataag tgaaatgatt cgctcttcat 8940

ctacttaaga ttgcgttata ttgctgacct ttctaatcgc ctaaccacga tcacatgctc 9000

ttccagtgcc ggtgagggtg catcaaatgc aactggtaat tcagttggca gaaaaaggat 9060

gagaatggat gggacttcaa cacaccacga ggcagttagc acgcaccctt ggaacaaggc 9120

tgaactttcc aacaggatcc aatgcatgac tcatcagtta gaagaggctg taaatgaggt 9180

tatgaggcta tgtcgatcct caagttcaaa ccagagtcga cagggtacac caccggccac 9240

aaatgcaaca acatcgtctt atcttccgga gcccatagtg tatgggaggg ctgcagagat 9300

ggaaaccatc aaacagctga tcatgagcaa tagatctaat ggcataaccg tcctgccaat 9360

tgtaggcaat ggagggatag gaaaaaccac tttggcgcaa ctggtctgca aagatctggt 9420

aattaaaagt cagtttaatg ttaagatatg ggtgtatgta tctgataaat ttgatgtagt 9480

taagattaca aggcagattt tggatcatgt ctccaaccag agccacgaag gaataagcaa 9540

ccttgatacg cttcagcagg atcttgagga acaaatgaaa tctaagaagt tcctcattgt 9600

cttagatgat gtgtgggaaa tccgtacaga tgactggaaa aaactactgg ctcctttaag 9660

acctaatgat caggtgaatt cgtcacagga agaggcaaca ggtaatatga taattttgac 9720

aactcgtata cagagtattg ccaaaagtct tggaacagta caatcaatta agttagaagc 9780

tctgaaagat gacgatatat ggtcactatt taaagtgcat gcttttggta atgataaaca 9840

tgatagtagt ccaggcttac aggttcttgg gaagcaaatt gctagcgagc taaaaggcaa 9900

cccactggca gcaaaaactg tgggttcact attaggaacg aatcttacca tcgatcattg 9960

ggatagcatt ataaagagtg aagaatggaa atccctgcaa caagcttatg gcatcatgca 10020

agcgctgaag ttgtgctatg atcatctatc caacccctta cagcaatgcg tctcttattg 10080

ttctcttttc cccaagggtt attctttcag caaagcacaa ctaatacaaa tatggattgc 10140

tcaaggattt gtggaagaat ccagtgagaa gttggagcag aaaggatgga aatatctagc 10200

tgagttggta aattcgggtt tccttcagca agttgaaagc acacggtttt catcagaata 10260

ttttgttgtg cacgatctta tgcatgattt agcgcaaaag gtttcacaaa cagaatatgc 10320

aactatagat ggctcagagt gcacagagtt agccccaagt atacgccatt tgtcaatagt 10380

aactgattct gcataccgca aggagaaata tagaaacata tctcgtaatg aggtgtttga 10440

gaaaaggttg atgaaagtta agtcaaggag taagttgagg tcactggtat taattgggca 10500

atatgattct cattttttta aatatttcaa agatgctttc aaggaagcac aacatctgcg 10560

actgctgcag atcactgcaa cttatgctga ttctgattca tttctctcca gtttggtaaa 10620

ttctacacat ctccggtatc tgaaaattgt gactgaagaa tccggcagaa ctttgccccg 10680

atctctaagg aagtattacc atcttcaagt actagatatt ggctatagat ttggaattcc 10740

ccgtatatct aatgatataa ataatcttct cagcctgcgg catcttgttg catatgatga 10800

agtgtgttct tccattgcta acattggtaa aatgacctca cttcaggaac taggcaattt 10860

tattgttcag aataatttaa gtggttttga ggtgacacaa ttgaaatcca tgaacaagct 10920

tgtacaactt agtgtgtctc aacttgaaaa tgttagaact caggaggagg catgtggggc 10980

aaaactgaaa gacaaacaac acttagaaaa gctacatttg tcctggaagg atgcatggaa 11040

tggatatgac agtgacgaaa gctatgaaga tgaatacggc agtgatatga atatagaaac 11100

agaaggggag gaactgtcag ttggtgatgc caatggtgcc caaagcttac aacatcacag 11160

taatataagc tctgaacttg cttcaagtga ggtgctcgaa ggtcttgaac cacatcacgg 11220

cctcaagtat ctacggatat ctgggtataa tggatctacc tccccaactt ggcttccttc 11280

ttcacttacc tgtctgcaaa cacttcatct agaaaaatgt ggaaaatggc aaatacttcc 11340

tttagaaagg ctagggttac ttgtaaagct cgtgttgatc aaaatgagga atgcaacaga 11400

actctcaatc ccttcactgg aggagcttgt gttaattgca ttgccaagct tgaacacatg 11460

ctcctgcact tccatcagga acttgaactc cagtttaaag gttctgaaaa ttaagaattg 11520

ccctgtactg aaggtatttc ccttgtttga gatttgccag aaatttgaaa tcgagcggac 11580

gtcgtcatgg ttgccccatc ttagcaagct taccatctat aattgtcctc tttcctgtgt 11640

gcacagttct ctgccacctt catctattgt ttccaaatta tcgatcggta aagtttcaac 11700

acttccaacg gtgagggggt catctagtgg aacattaata attggactgc accccgatga 11760

agttgatgat gatgatggtt tggaggattc tgatcagctg aaaacgttgg atgacaaagt 11820

actattattc cataacctga ggttcctaac tagcttggca atatatggtt gtcgaaatct 11880

tgcgactatt tcaattgaaa gtttaaggca actcgtttgt ttgaagagtt tggaattata 11940

cggctgccca aaacttttct cttcagatgt tccaccagag cttacatgtg aatatatgtc 12000

aggagcaaat cacagcgccc tcccatctct cgaatgtctc tatattgagg attgtggaat 12060

aacggggaag tggctgtctc tgatgttgca acatgtgcag gccctacagg aactgagttt 12120

agaggactgc cagcagataa caaggctatc gataggagag gaagaaaaca gtcaaccaaa 12180

tcttatgtca gctatggagg atccgtcatt aggatatcca gatcgagacg aacttctgcg 12240

ccttccgtta aatctcatct cttctctgaa aaaggtatct attacatatt gctatgattt 12300

aacattctac ggcagcaagg tagatttcgc tggatttacc tcccttgagg agttagtgat 12360

ttcacgatgc cccaagctgg tgtcgttctt ggcgcataac gacggaaatg atgaacagtc 12420

gaatggaaga tggctcctac cgctatcact tggaaaactt gagattaact atgttgattc 12480

cctaaaaacg ctgcagctct gctttccggg gaacctcacc cgcctgaaaa aactagtagt 12540

gttgggaaac caaagtttaa catctctgca gctccattcc tgcacagcac tccaagagtt 12600

gataattcga agctgtgagt cgcttaattc tctggaaggc ttgcaattgc tcggcaatct 12660

caggttgctg tgtgcacaca gatgcctcag cggccatgaa gaagatggaa tgtgtatcct 12720

tccgcaatca cttgaggaaa tttacatctg cgagtactct caagagaggc tgcagctctg 12780

ctttccagga agcctcaccc gcctgaaaaa actagtagtg ttgggaaacc aaagtttaac 12840

atctctgcag ctccattcct gcacagcact ccaagagttg ataattcaaa gctgtgagtc 12900

gcttaattct ctggaaggct tgcaatggct cggcaacctc aggttgctgc aggcacacag 12960

atgcctcagt ggttatggag aaaatggaag gtgtatcctt ccacaatcac ttgaggaact 13020

ttacatcaga gagtattctc aagaaacgct gcagccctgc tttccaggga acctcaccag 13080

cttgaaaaaa ctagaagtac agggaagcca aaagttaata tctctgcagc tgtattcctg 13140

cacagcactc caagagttga tgattgaaag ttgtgtgtcg cttaattctc tggaaggcct 13200

gcaatggctc gtcaacctca ggttgctgcg ggcacacaga tgcctcagtg gttatggaga 13260

aaatggaagg tgtatccttc cacaatcact tgagggactt tacatcagag agtattctca 13320

agaaattcta cagccctgct tccagacgaa tctcacttgc ttaaaaagat tagaggtatc 13380

aggcactgga agtttcaaat ctctggagtt gcaatcatgc actgcactcg aacatttgaa 13440

gattgaaggt tgttcatcac ttgccacatt agagggcttg cgattcctcc acaccctcag 13500

gcatttgaaa gtacacagat gtcccagatt gcctccatat tttgagagtt tgtcaggaca 13560

gggctatgag ctatgcccac gactggaaag gctcgagatc aattatccct caatccttac 13620

cacgtcgttt tgcaagaacc tcacctctct acaataccta gagctttgca atcacggatt 13680

ggaaatggaa agactaacgg acgaggaaga gagagcgctt caactcctca cttccctgca 13740

agagctccga tttaactgtt gctacaatct cgtagatctt cccacagggc tccacaacct 13800

tccctccctc aagaggttgg agatctggaa ttgcgggagc atcgcgaggc cgctggaaaa 13860

gggtctccca ccttcgttgg aagaactggc tatcgtagat tgcagtaatg agctagctca 13920

gcagtgcaga ttgctagcaa gcaagcggaa ggtcaaaatt aatcagagat atgtgaattg 13980

attactcggt ggctttttcc acctgcccaa ctggcatggg ctcgttcagg cgttcaagct 14040

gctgtaaatt ccattgccgc aatgacgacc ttcagaaccg ttacacaata caaaggacat 14100

atgatggcta gatcaactgt cacatacaaa ttctataatt ctatctactg aaaaggatga 14160

ttgctgttca ttttcgtgat tacagaagga actgtgtata tcgtgctatt tttgcattca 14220

cattgtgttc caggttgtgc tcggatcagc caatttcggt ggttaattca ctttgctgtg 14280

tcctctgtgc agtgtgcact caagaattgt tcgcacatgc aattcagttg agcatcgcac 14340

tacgcaagtt tttttttttt tgttaacaaa ggagtggttg acagcattgc aggttgctaa 14400

aacagtgtca aaaatttgct aacaaaagaa ttctcttcag aaatagtatg aaaataaatg 14460

ccacataagt aatctgagta aaacagacag aatcactaga gaagactaac gacaaactct 14520

tctttttcta tttctgtggt gaaaaactca gcatataatc tcatgtatgc attggaggtg 14580

cataccattt cgatcatgta tgcatttagc tgaacattat caagaagcaa atttattgca 14640

cgggcaacca taagctaaac taattctcaa gcaatttaat aatcacaaaa cagtctgcaa 14700

taccaagata tataaaactt ttgccagcat ggagcaacaa cactgaaaca ctacagtcaa 14760

gacagctccc agaaaagatt atatgttgct acttcaattg ccacagttac aatcacccat 14820

aatgacacat caaattacat aagagtatta agagtatatg acagttgtaa caaaacaact 14880

gcattgagaa ctagaagaac agacagccac atgaaccata ttcacttctt cagatcagaa 14940

agcctggtga actaccagtc taccaattgc taccgtagtc tccatttttc acttatatgt 15000

acataggaca cctgcgctat cagaatagcc acaaagccag ttcactcgct acaaattaat 15060

cttgactaaa tccaaacaga taccaactcc tcttaatcaa cttacaaaac acctacactt 15120

ttgttgtctc tacaccacca atcacacaaa aggaaattaa tcatccacct actcgtatcc 15180

tactgttaga atggaggcct tacaccagag aatgggcatt gccagattct gagacactgt 15240

cctttgagct ccctgaatct gaaccagtaa caaaaatcaa ataagacaca acccaaaacc 15300

ttccgttcat caagaagttg gatgttcaaa ttataaagtg acagttcgat cgtaccactg 15360

gatgatgagg acccactgct cgcggcctca gtatccttct caatctccac tgattggtaa 15420

gttgctgttg gcatttcatc cccgacatct acacaggggc ggacccaggg ctggtgccgg 15480

gtatgcaccg gcatacccag aaaattcgga aaagatttag taggcagtat gcatcagtgg 15540

gctgaaacta agcaaaagcc caagaagtaa aagtctagct gttgcacagc ccaccagaag 15600

atgatgttcc cctttggcta gcactcatgc ggtagggttt ggagcaggcg ttggcggcgc 15660

tgcgacagcg tggcgcgctg tggcactgta tcattgatcg gcagcctcat ggcaagcacg 15720

acgcatggac ggtttgagcg tcgtggcgct gcggccggac agcgagcacc ggtggcagag 15780

gctgccggtc actgggctag gcgctgacta aatcacctgg tccagttcca ctcattatct 15840

gtccatgtcc aagtatactc aaacttcttc agttctttgt ctctctgatt tttttttctt 15900

aacagatgga gacatagatt gattgaaaat tgaaaactat aatgagctaa caagtgaaat 15960

caatgagagt acaaacttca aaaattgaat acatgttttt gtgatttgta cgctgagatc 16020

aagttgaatc aatcagatta gaatacgaag aagaatatgg gtattctttt tctttcttta 16080

gcaaagcacc attagattct gcttcatcag gccatgccct atgcttcaca tttctaatgg 16140

tgatgggaaa ccttagaaca gggagggaaa gtaactgaac aaagtgagtt gtgttatttt 16200

gttcttcaat tcaccctcag gatacaccaa tactttccta aaattaagac tatatttgtg 16260

tcatcatgtg catatagtgt ttgatttgcc tctcttgata gctagcatgg gcaaacccag 16320

gacaaaaatt ctgggtccgc cactgcatct acatactcat ccaactgctc ggcctgtgta 16380

gtgatcttgt caggatcctt gctctcctgt aaacaacaaa acacatctat tatacaatta 16440

aaggaataga aaaaggagcc tccacgttcg ctctcatggc ctagaaattc tcacattaat 16500

cgaagaaaaa gaaaaacaga gtccatatat agaaatacaa tttagaaata gttgaaattc 16560

gaaattaaaa aataaggaat attagaagat gagactagag tccatataga aatacacttt 16620

agaaatagtt gaaattcgga attaaaaaat aaggaatatt agaagaggag tatagagtcc 16680

atatagaaat acaattagga aataatagaa attcggaatt aaaaataagg aatattagaa 16740

gtagagtata gagtccatat agaaatacaa ttaagaaaaa aaatagaaat tcggaattaa 16800

aaaataagaa atattagaag tagagtatag agtccatata ggaatttaaa actaactaaa 16860

attcggaaaa aaaaaagaag ccaccacgtt cgctctcatg acctagaaat tctcacatta 16920

atcggagaaa aagaaaaggc agagtctata tagaaataca attcagaaat agctgaaatt 16980

cagaattaaa aaaataagga atattaaaag aggagactag agtctatata gaaatacaat 17040

ttagaaatag ctaaaattcg gaattaaaaa ataaggaata ttagaagagg agactagagt 17100

ccatatagaa atatgattca gaaatagctg aaattcggaa ttaaaaaata aggaatatta 17160

gaacaggaga ctagagtcca tatagaaata caattaggaa ataacaaaaa ttcggaatta 17220

aaaataagga atattagaag tagagtatag agtccatata gaaatacaat taagaaataa 17280

ccgaaattcg gaattaaaaa taagaaatat tagaagtaga gtatagagtc catatagaaa 17340

tacaattaag aaaaaaaata gaaatttgaa attaaaaaat aaggaatact ataagtagag 17400

tatagagttc atattggaat ttaaaaccaa ctaaaattcg gaataaaaag tagcctccac 17460

gttcgctctc atggtctaga aattctcaca ttaatcagaa aaaaagaaaa agcagagtcc 17520

atatagaaat acaatttaga aatagctgaa attcagaatt aaaaaataag gaatattaga 17580

agaggagact agagtagtta ttatacatta gtagttttga aaagttattg caaaatttaa 17640

aattatgttg tcattgtaat atattttaat aatataatga gaaaatatat atgatattat 17700

ataagagaaa atataatgat gctagccgcg taatctacgc ggaccaccat gctagttaac 17760

cattataaga gctcatttca cacataagct aatagcaacc aagaacaaag catgctgctc 17820

actattgcca tcgtcacatc accgttgtcc accatcgtag cggtactgcc attcaccagc 17880

atatcactat cattagccac ggaagcatca atgacatcgg cattttcacc attaacaatg 17940

gcagcccgtc gactcttgtt gagcgccttc ttgaatttgt tgacgaatcg atcaagctcc 18000

cactgtgtct cgacatccat ctcatcaata tccagctcga tctcaccccc aaccagctca 18060

ggattgccat tcctcttccg cacaatctgc aacacattat gcatcttctc ctctggcaag 18120

ctctccaacc caaccctcaa caagttcttc tcctccaggg tcatctccct cttgttcggc 18180

tcccttgcct tcgttttccg cattttcaca ttccctgccc ttggtttcac ctgcgccgga 18240

gctttcgccg gtggcaattc cggtggcggc actggcatcg gcggctcaag gatcttgagt 18300

tcctgctcga accaagacac cgacgctttg tacatcttct caaaggacgc gaggaggtcg 18360

ccggcgaagg tgtggacctc gtgtccagca gggttgtacc gcagcgcgtt gctgaacgtg 18420

agccggatgt cggcggcgaa gtcgtcgtgc gaggggtacc ttccggcggc gaggttcgcc 18480

ctcaccgtgc cgagatccat ggggcacttg atgacggcgt ggtagtcttg gagtccgagg 18540

cggtcgacct ccacgggggc gttgaaccag atgctccgct tgtccttccg cagcttcgcc 18600

aggatctggt cgcaccgctt ccgcatcgcc gccctgagct tcgccggcgg cggggggagg 18660

ccccggcgag gcgagccatg ggcgcttcgg cggcttctgc tgttgctgac cccacgagcc 18720

aatgcgggag atgaggcgcg gaccaggccg aggcccaact cgcctgcgac tccgcgaggc 18780

ccatgagagt tcgtttgggc tatcagtcca ttacggacaa gaagcccgag ggcgcggccc 18840

acatccttcc gtcttccccg cctttccttc cctgcctcgt cgaccgcgtt atcccgtctc 18900

aagtctcaac tcccaacctg cgatgcgccg tagaaatttg atactcgtgt cactcgtcgt 18960

cttcctttgc ttcactaatc actccaatga cgcgacacgc atctctctca aaaagaaaag 19020

gattggtatg taactgtcta caagtgatga attcatcact cacgcagttc actgcacatc 19080

gtcccagtac gcgcactatc ttggcagcaa aaagttgtac cagaatgatc ttgcgttctt 19140

ggcaggacat gatcgatcaa aataatctca agcactttgg ctacacacaa tgaacgaaat 19200

tttgcagctc ggcagctcac caaggatggg cgcaactcaa ccacgactga aatcggtgct 19260

gacactttta gcagcatctt tgtgattcag ccgtggtttc tccggcgcgt ctccttctgt 19320

tcagagaaaa ccgaatagtt cgggttcgga ttcagttcga agcaagcatg gcttaaaatc 19380

agttcatgca cacacagctg cccccttggc atttgtgtgc actgacacga tgccgtctgt 19440

cctacgcggg ttggtagtat gatgaaaggg tttggaaacc gatctaaaaa accgttcgga 19500

tcatcgggtt tttgactagc catgaaagtg aaattgtccg aaaaggataa aagagcgaac 19560

gacgtcgtcg gtccagcaca aagctcagcg ctcaatcacg tcagagaaac cgatcacttc 19620

gggtccgggt tcggttctgg agagctaact aaggacttac ggagtggtat gttcaactgt 19680

tcagagtatt taacttcacg gccaatacgt tgtcgtccac cagagtgcat gtgagcgtca 19740

cgacgcatcg gataatttga ccattcgtag gttgctgttg gcgagcacgg cgaaacccga 19800

gacgcgggtt cggtttcggt taatccgttt cgcggtcgcg cgcggccacg ggcacacggc 19860

catggcgcca tgccgctgtc tccccctcgc tggctgcgac gcttgactat ttttattctt 19920

catctccatt tgattgattt aatctctcgt tgcagcagca ggcaacgtgc aatcggttcg 19980

gttttgctac tgttcgggtt cccctggccc tctcccccct ataaatacac gcctccaaag 20040

tccaatccaa tccgccatga acactcggtg ctcgagccat ttccaattcc aagcttacat 20100

cgaatcacca tccacgacaa agaagacgca tacgctggtg atggagctca tggcctccct 20160

catcaacatc ttggcgctga tctccgaggc atgccgcagc gcggagaagc tgccggcggc 20220

gctgatcact ggcggcgtcg tggaggccgc ggcggcgatc ttcgtcgcct tcttcaagcc 20280

gcccggtggc gtatttcagc accacggcaa ggcaccattc tacctgtatt acggcattat 20340

aggaggcgtg gccatcttcg gcttcgcgga ggcgtgggcc gggttctggg tctccggcga 20400

cctgaacggc cggcgcgccg tcggaaagac gatactgtgg gtgtcgattt tgcctcttgt 20460

cttggtagct gcgctcggag ggttcgtctt catgagatag ttgcaaacct atgttaattt 20520

cctctgtttg tacgagtggg tgtaatctcc tgtacctctg tgtgtagtag ttgtagcgtg 20580

tatgcactag tttttgcttt gtaattttta gcctggtgta tttatgatat ttcagttatt 20640

atgtgatgta acagtatgat tgttaacttt tacaaggttt tatgtaatta tacataaggt 20700

tattttttgt gtgatactcc ctccgttttt taatagtgac gccgttgact ttttctcaca 20760

tgtttaacca ttcgtcttat tcaaaaaatt tatgtaatta taatttattt tgttatgagt 20820

tattttatca ctcatagtac ttcaagtatg atttatatca tatacatttg cataaaattt 20880

ttgaataaga cgaatcatca aacatgtgag aaaaagccaa tggcgtcata tattaaaaaa 20940

cggaggtagt atgtgattat gcgcgttttc cttgcatttc tttagctacg gatgttgtac 21000

aggtgtacct cttttatgag tattcctcca atcttctgct tatatccttc tcgaaatcat 21060

ggcagtaatc aaccaatcat aatcttaaaa gaatcatggt agacgcagag caaccagtta 21120

gtacatattt gaaagaacag gcccttgcat tgcagggccg tatcctgtag gctgcggtgt 21180

ttcaaagcag gaaaaagtac accgaaggtc cctcaacttg ttatcgagtt acaaaatcgt 21240

ccctcaaccg caaaaccata ccggacgtcc ctcaactaac aaaactgttc actttaacgg 21300

tggttttgac cccggtttta tttgaggtgg cggctgagtc agcgtgggac ccacgtgggc 21360

cccacatgtc agaatgccag gtcgtctctc tctcctcttc tctcccttcc tcgtctctcc 21420

ctctcacttc tctcctctct gcagggcggc tggcgggtgg ggaggaggtc cggcagccag 21480

cggcggcgcg tgcgacctcg gggtcggacc gtcggagagg cccgggaggc ggctgcggcg 21540

gcggcggcga cgcttgagaa gggagaggcg gccggtggcg ccggcgcggg aagaagctgt 21600

cttcggcgtc gtgcccgctc tgccctccac cgcctgcttc gcccgccgct gccccgcgct 21660

cacacactcc cgccggcgac gccgctgacg gcgcgggcgc gagaactgcg gccatggatg 21720

acgacggcgg cgacatcctc gctccccccg ttccgctccc cgtcggcgcc atcttcctct 21780

cccgcagcgc gcctgcccgc cgctcgtctg ctccgtcggc cggccgctcc ccgcgcagtc 21840

ggcggtggcg aaggtgaccg tgccggcgtc gaggtcgtag ccgacgtgga tgttctgctg 21900

cgccaggttc ccaaggatgg acaccggctg ctgcttcgtc gtcgccacga ttgccaggca 21960

cagcgtcccc tcctacaccg tcacggacgc gttctccggc ttcagcgcca ccgccgcgcg 22020

gccgccgcca ccgccgaact ccagcgtcaa gtccggactc gcttgttcgg tttgcttcct 22080

gctggatgta cacatgaggt cattcaagat cagtaactca tactctattc tagcaagctg 22140

gctgttggtg agaattctga attctcattg catcgcctaa cgaagataga agatcggagt 22200

gttatacatc aatcaatttc ttagctggga atgttactcc tgcattgctc tacaatttat 22260

gggtcatgta atttgatctc tcacagaatc tatagcgagg taagaaagcg aaaccggcgc 22320

cacaaaatat cttgaaattc tgcttgttca tacactgttc ttctgattaa agaacaactt 22380

agcttgagat tgttctgttc acttgcaatt tgcaggagta acaaagctta tttttgcatt 22440

gcccgagtcc acaatgatgc gggagctcgc cgccgatgcc accgtcttgt tgccgaccct 22500

gacgaagtcg aggaccacgg tgtagtacgt gtccacgtcc ccggcgacga gcggcgtgct 22560

cgctgcgccg agctcggtga cgttggcgag ggtgccgaag ttgaacgccg acgatgagtt 22620

gacggagtgc gggacgaggc tgtaggagaa tctccggccg agcgacgcct cgccgccgag 22680

ctgcgtcacg agggagaccg cgtcgccgct gagcccgacc agcccgtcgg ccgggaacga 22740

gccggccgtc gtggtggagc agccgaactt gacgctgcac acgccgcctc gtggtcgtgg 22800

gcctcgtggg tggcggccgg cgggggagcc cgcgagccgc tcctacccct gccgccctcc 22860

accactgccc gcccgcaggt cctcccccca ctgctgccgc gtcgcccgtc gccgccacca 22920

ccgcagccgc gtcgccatcg aatcggggag aagtcagaaa gacatgagga aaggagagaa 22980

gaggggagga gagagatgac ctggcatcct gatatgtggg gcccacgtgg gtcccacgct 23040

gactcaaccg tcacgtcaca caaaaccagg atcaaaacca ccggagaatc taaagtgaac 23100

ggttttatta gctgagggac gtccggtatc tggttttgtg gttaagggac gattttgtaa 23160

ctcgatgata agttgaggga ccttcggtgt actttttcct ttcaaagcat aatgaagaaa 23220

tcaaacttgt caggtctcag atggctcgca agcagttctc cggaaaagga ggagagaaga 23280

agatatggac atacggggtt aatactaatg cattagcata agctgttcag ttccgggttc 23340

ggttgtgaca tgtactgatt tctttgttaa tagttaatca gcggaaatca tgtctttttc 23400

ttattggtgg gtccccgact gtggatcttt ttgcgcgcct ctcttggttt ggattgcaaa 23460

gaagcctacc gacccggttt cgggttcggg ttcgggttcg ttgctcagcg cttgtgttgc 23520

tttgctcagg catactgcat acatatgctt tcagaatgtc tctatcaatc cgggttgaaa 23580

aaagtggtct tgttgagatg aaattgctta tcggcaccat tcgggttcgg ttcaagtcgt 23640

cggtttcctg tactactgct agtcagaaaa ccgagctacg tggccgatcg ggttcgggtt 23700

tccgagagca aacaacgtag cgtgtgctcg tcattttgca atcgctccac ttgctgcgag 23760

ctcggcgaac ggttaatctg gacgccatcc ctacgaaaac ccgagacgcg gtctggcggg 23820

ggtcggtgct tcggttcggt tcatggtata gatggccttt cggcccgccc ggcacggccc 23880

ggcccaggaa cggcccacca gccatcgggc cggcacggcc cgatcggcac ttcgtgccgg 23940

gccgtgccga cccatgggct gcacctcccg cccaggcacg gcccaccagc cgtcgggccg 24000

tgccgggccg gcccgaaggc gcgggtggcc catcgtgcct ttttatataa gtctatattc 24060

catccctcct ctttgggccg tgaaatatat atagcgaaaa taagtctatt tgccgtccct 24120

cttctttggg ctgtggcata tatatagtga aaataagtct attttctgtc cctcctcttt 24180

gggttgacat atatatatat atagctaaaa taagactatt ttacgtctct attctttcga 24240

ccatggcata taatatgtct atttttactc cctattgttt atgtgtcggg cctagcctga 24300

ggcccatcgt gccgtgccgg cccggcacgg ccgggccggc ccggtagtcg ggccgggcca 24360

gcacgggccc aacccctaac gggccgtgcc gtgcttgggc cgggccaaaa cgccgtgccg 24420

tgggccgggc catcgagcgt cgggcctttt ggccatctat agttcatggt gtgtgggctg 24480

tgttccctat gggaccattc gggtgattta attagcacga aaaacggagt agttaattag 24540

cacgttgatt aattaagtat tatttaattt tctttttaaa aatggatcaa tataaatttt 24600

ttaaagaact ttcatacaga aattttttta aataaacata ctgtttgtca gtttaaaaaa 24660

cgtacacgcg gaaaacgaga aaggagtgtt aagaatttgc tcttgcaaac acagccatgg 24720

atggacacga tggatcatgg atcgctgcga cttgcccgcg aatgaatctc cgtcgagggc 24780

tcgaggcagt cctgggtttg gctttgtgcg tcgggtttcg ccggtgtatt aaacaggcac 24840

aagcataaaa ctccaagtca ccgagcagct ccgatttttt tatttttagt ttttattaaa 24900

atatttacaa aactaatttt gtgttttaaa aatttacaaa actagtcacc catcgcccgt 24960

ggcagatggc agttgcctca tgcctaacgg gcggcaaaga agggcgtgcc attttgcaga 25020

tggcccccct tgctgccctt aggttataaa taccaaagag gccgcttcca aaatgccaag 25080

ccgtcctcgc cgcccttttg caggcggccc ccgcacagta gaaaatcgcc ctctataagg 25140

gtggcaaggg ggccgcctgc aaaaatgtcc ctacggacgg caggagggta gcctgcaaaa 25200

tggcaggcct tcctttgccg gccgttagac atgaggcaac gatcgtctgc cacgtcatta 25260

aaatcgatct ttaggaggga tttatggacc agtaagtcca acgtgtgcaa agtattccac 25320

ccgaaaaatc cgtcgggaaa ccgatagagt atatgcgtgg ccgttcgggt ttatcatttt 25380

gtatttcttc cgcttgcgcg cgttggcact gcactgctca ctacgctctc ggcgagtgat 25440

tactctggtc gcgcgacaac ccgtaacgcg atctcgcggt cgcgggttcg gtttcggttg 25500

agctgtggtg tggatcgctg caacttttgc ttgaaaaatg aaaaacatgt tcgtccgcga 25560

ttttctgtcc gtcgcctctc actgatcgag agatgcaaca cagtctgagc gatcgggttc 25620

ggtttttgtg ctctgctttc accatgaagt catcaccagg ctcactgcgt ctgcctcctc 25680

gcaccattca gcattcaccg agttgaaaaa accacaaact aaggcgttcc ccctgatgat 25740

acagcacgcg agctacgcac gcacagacat acggtacaga catgccggcg cccgcatcga 25800

cgtcgagaag ctgctcactc atcccgtgcc ggcgtcttgc aagtcgcggc ggcggcgctc 25860

tgcctgggct tacggcccaa attcggcctg caggcccgat tttgagttgt caagtcagag 25920

cccaccgact tggaggcagt cggcccaagc atttgaaagc ccagaaacat ctgctccgag 25980

tgctcacctt ccgtgtagct tcctagtagt tcctacacta ccaaactacc gcattattta 26040

tatccctcac aaaaaaaaaa taaaaaggac tacccgcatt ttatattgct catctgccct 26100

cctcgaatac ctcccatatt attcccatct ctgccttttg cttaccactg accactgtct 26160

tctcgcgagg ctcgccactg gtccattgtc gcccaagctc caaaggcttc tcttccgccg 26220

gatctgatgg aggaggtgga ggtcggtttg ctggagggag ggatcgggtg gctggtgcag 26280

accatcctgg agaacctgga caccgataag ctgggtgagt ggattcgtca ggttgggctc 26340

accgatgaca ccgagaagct caggtcagag atcgagaggg tggaggtggt gacggctgcc 26400

gtgaagggga gggcgatcgg gaacaggtcg cttgcccgat cgctcagccg tctcagggag 26460

ctgctctacg acgccgacga cgcgatcgac gagctcgact actacaggct ccaacagcag 26520

gttcaaggag gtaaagcttg tgtatgcaag atgtatccat ttttgtgtcc aaggaggtgc 26580

attcattccc ctttttctgt tgtttcagat gcatggcaag gtggcactgg aagtttagat 26640

gaacctgaag cagagcaagc agagagaccg agtatcaacg ctgctattgc gattagcagt 26700

ggtagcaaaa agcggtccaa ggcatggggg cactttgata tcactgaaga agaaaatgga 26760

aagcctgtga aggcaaggtg tattcactgt cacacggtgg tcaagtgcgg ttctgaaaaa 26820

gggacatcag ttttgcataa tcacctcaag agtggcagct gtaacaagaa gcgtgaggca 26880

actgatcagc agccaaaccc gtcatcaagg tacggtatat gatttatgta acgtactttc 26940

ccccgcagca agccagcaac tgtgaaaccg ttctgttttt ttttaaaaaa tatttttatt 27000

ttgcagtact gctgatactg cagcaaatag cactcttgtt gaactcggcg gttcaggttc 27060

agacatcaga aaaaagatga ggattaatgg tgagtcaaca cacaacgatg caccttatgc 27120

acacccttgg aaaaaggctg aatgttccac aaggatacag caaataactc gtgagttaca 27180

agatgcacgg ggggctgtga gtgaaattct taagctacat ggaccgtgct ctgttggaaa 27240

ttcaaaccat cgtacgagta caaccacaac tctctgcaga agaacgtcaa gtcttaatcc 27300

acacaaaata tatggaagag acgcagagaa gaacaccatc atgaagatta ttacagatga 27360

cagttatgac ggagtaactg tagtccctat tgtgggcatc ggaggagttg ggaagacagc 27420

tctcgctcaa cttgtataca acgaaccaac ggtgaaacgt gactttgagc ggatatgggt 27480

ttgggtgtct gataactatg atgaattgag gatcacaatg gagattctag attttgtctc 27540

tcaagaaaga cacgaagaat ctccctgtag aaaagaaata cggaaaggag taagtagctt 27600

tgcaaagctt caggagattt tgaatgggta tatggacatc cagtcgaaaa agtttttgct 27660

tgttttagat gacgtatggg acagcatgga tgattacaga tggaatattt tgttggatcc 27720

attgaaatca aatcatccaa aaggtaatat gatccttgtg acaactagac ttttgtctct 27780

cgcacagagg ataggcacag tcaaaccaat cgagttaggt gctttgtcaa aagaggattt 27840

ttggttgtat tttaaaacat gtacatttgg tgatgagaat tacaaagcac atccaagttt 27900

gaacatcatt gggcagaaga tagctgacaa gttaaagggc aatccattag cagcaaaagc 27960

aacagcgctg ctattaagag aaaaacttac tgttgatcat tggagcaaca ttctgatgaa 28020

cgaagattgg aaatccctgc atttcagtag aggcatcatg cctgctttga agcttagcta 28080

tgatcagctg ccttaccatt tacaacagtg tttgttgtat tgttccatat tccctagtag 28140

ttatcgcttt gtcagcaagg agttgatctg tatttggatt tctcaaggct ttgtgcattg 28200

caactcttca agtaagagac tggaggagat agggtgggac tacctaactg atttggtgaa 28260

ctctggcttc tttcagaaag ttgatcatac acactatatc atgtgtggcc ttatgcatga 28320

ttttgcaagg atggtttcaa ggactgagta cgcaactata gataatctac agagcaacaa 28380

aatactgcca actatacgtc atttgtcaat actaaacaat tctgcacact atgaagatcc 28440

tagtaacgac aaggttgaag gaagaattag aaatgcagtt aaagcaatga aacatttgag 28500

gactttggtg ctaattggga aacatagctc tttattcttc caatccttca aagatgtagt 28560

ccagaaggga catcatttac gtctgttgca aatctctgaa acatgtactt atgttgaccc 28620

cttgctttgc aatctggtga atccagccca tattcgctat atgaagcttc acaaaagagc 28680

tttgcctcaa tctttcagca agttttacca tcttcaagta ttagatgttg gctcaaaatc 28740

tgatctgatt atacctaatg gtgtggatga tctagttagt ctgcagcatc ttgtagcagc 28800

agagaaagca tgctcgtcca tcactagcat cagcaaaatg acctctcttc aggaactaca 28860

taactttggt gttcaaaatt ctagcggctg ggagatagca caactccagt ccatgaacca 28920

gcttgtacag ctcggtgtgt ctcaacttga aaatgtcaca actagagctg aggcttgtgg 28980

tgcaaaacta agagacaaac agaacttaga aaagctgcgc ctttcgtgga ctaatttaca 29040

taaattgggt catttgggga ctaacgtgcc atgggatgaa cgtgaaaatg caagagcagt 29100

gcttgagggt cttgaaccac atacaaatct taagcaccta gagatatatt cgtacaatgg 29160

tgctacccct ccaacatggc ttgccacttc acttacctct ttacagactc tccgtctaga 29220

gtgttgtgga caatggaaaa tgattccatc actggaacgt cttccctttc ttaaaaagat 29280

gaagttggag agtatgcaga aaataataga aatgacagtt ccttcgctgg aggagctgat 29340

gttaattgac atgccaaatt tggagagatg ctcctgcact tccatgaggg acttaaactg 29400

cagtttaagg gttctgaagg ttaaaaagtg ccctgtgctg aaggtctttc cattgttcga 29460

ggactgccaa aaatttgaaa ttgagcggaa atcatggttg tcccacctta gcaagcttac 29520

tatccatgat tgtcctcatt tgcatgtgca caatcctctt ccaccttcta ctattgtttt 29580

ggaattatcc atcgccaaag tttcaacact tccaacgttg aaggggtcat ccaatggaac 29640

attaacaatt tggcttccca atgatgatga tgttcctgat aagctgataa cgttggatga 29700

taacattatg tcgttccata acctgagttt cctaactgga ttggaaatat atggtttcca 29760

aaatccgacg tctatttcat tccatggttt gaggcaactc agatgtttga agactttaaa 29820

aatatacgac tgcccaaaac ttctcccttc aaatgttcca tcagagctta ccggtgaata 29880

tatgtcagga gaaaatcaca gcgcccttcc atctctcgta cgtctccata ttgagaagtg 29940

tggaataatg aggaagtggc tgtctctgtt gttgcaacat gtgcaggccc tacaggaact 30000

gagtttagat aactgcaagc agataacagg gctatcgtta ggacaggaag aaaacaatca 30060

accaaatctt atgtcagcta tggaggatcc atcattagga tatccaggtg aagataaact 30120

tatgcgcctt ccattaaatc tcctctcctc tctgaaaaag gtatctatta cattgtgcaa 30180

tgatataaca ttctacggca gcaaggaaga tttcgctgga tttacctccc ttgaggagtt 30240

agtgatttca cgatgcctca agctggtgtc gttcttggcg cataacgacg gaaatgatga 30300

acagtcgaat ggaagatggc tcctaccgct atcacttgga aaacttgaga ttaaacatgt 30360

tgattcccta aaaacgctgc agctctgctt tccaggaaac ctcacccgcc tgaaaacact 30420

agtagtgttg ggaaaccaaa gtttaacatc tctgcagctc cattcctgca cagcactcca 30480

agagttgata attcaaagat gtgaatcact taattctctg gaaggtttgc aattgctcgg 30540

caatctcagg gggctgctgg cacacagatg cctcagcggc catggagaag atggaaggtg 30600

tatccttccg caatcacttg agaaacttta catctgggag tactctcaag agaggctgca 30660

gctctgcttt ccaggaaacc tcacccgcca gaaaatacta ggagtgttgg gaagccaaag 30720

tttaacatct ctgcagctcc attcctgcac agcactccaa gagttgatga ttcgaagctg 30780

tgaatcgctt aattctctgg aaggcttgca atggctcggc aacctcaggg tgctgcgggc 30840

acacagatgc ctcagtggtt atggagaata tggaaggtgt acccttccgc aatcacttga 30900

ggaactttac atccatgagt attctcaaga aactctgcag ccctgctttt cagggaacct 30960

cactctcctg agaaaattac aagtaaaggg gaactcaaat ttagtgtctc tgcagctcca 31020

ttcttgcaca tcactccaag agttgataat tgaaagctgt aagtcaatta attcgctgga 31080

aggcttgcaa tcgcttggca acctcaggtt gttgcgggca ttcagatgcc tcagtggtta 31140

tggagaatat ggaaggtgta tccttccgca atcacttgag gaacttttca tcagtgagta 31200

ttctctagaa actctgcagc cctgcttcct gacgaatctc acctgcttaa aacaattaga 31260

ggtatcaggc accacaagtt taaaatctct agaactgcaa tcatgcactg cactcgaaca 31320

tttgaagatt caaggttgtg cgtcgcttgc tacattggag gggttgcaat tcctccacgc 31380

cctcaggcat atggaagtat tcagatgccc tggcttgcct ccatatttgg ggagttcgtc 31440

agagcagggc tatgagctat gcccacgact ggaaaggctc gacatcgatg acccctctat 31500

ccttaccacg tcgttctgca agcacctcac ctccctccaa cgcctagagc ttaactatcg 31560

cggaagtgaa gtggcaagac taacggatga gcaagagaga gcgcttcagc tcctattgtc 31620

cctgcaagag ctccggttta agtcttgcta cgatctcgta gatcttcctg cggggctcca 31680

cagccttccc tccctcaaga ggttggagat ctggtggtgc aggagcatcg cgaggctgcc 31740

agagatgggc ctcccacctt cgttggaaga actggttatc gtagattgca gtgacgagct 31800

agctcatcag tgcagaactc tagcaagcaa gctgaatgtc aaaattaatg gggaatatgt 31860

gaactgatta ctcggtggct tgttaggcgc acctttttcc acctgcccaa ctggcgtggg 31920

ctcgttcagg cgttcaagct gctgtaaatt ccattgccgc aatgacgacc ttcagaaccg 31980

ttacacaata caaaggacat atgatggcta gatcaactgt cgcagagcta gctttggttc 32040

acctgaaaac ataaggccaa acgcgtggtt cttttaatca gaagtatcaa aaattggttt 32100

tggtttttaa cgaaaagatg ttagaaattg gttttaatgt gccagattct gtaccagaat 32160

tctgtatttt gctccgtaat tctgtgctac gactgatgtt gtatttaact gatagcaatc 32220

gaccggcaaa accaatccgc tccaatgcat tgtcacatac aaattctata attctatcta 32280

ctgaaaagga tgattgctgt tcattttcgt gattacagaa ggaactgtgt atatcgtgct 32340

atttttgcat tcacattgtg tcccaggttg tgctcggatc agccaatttc ggtggttaat 32400

tcactttgct gtgtcctctg tgcactcaag aattgttcgc acatgcaatt cagttgagca 32460

tcgcactacg caagtttttt ttttgttaac aaaggagtgg ttgacagcat tgcaggttgc 32520

taaaacagtg tcaaaaattt gctaacaaaa gaattctctt cagaaatagt atgaaaataa 32580

atgccacata agtaatctga gtaaaacaga cataatcact agagaagact aacgacaaac 32640

tcttcttttt ctatttctgt ggtgaaaaac tcagcatata atctcatgta tgcattggag 32700

gtgcatacca tttcgatcat gtatgcattt agctgaacat tatcaagaag caaatttatt 32760

gcacgggcaa acataagcta aactaattct caagcaattt aataatcaca aaacagtctg 32820

caataccaag atatataaaa cttttgccag catggagcaa cagcactgaa acactacaat 32880

caagacagct cccagaaaag attatatgtt gctacttcaa ttgccacagt tacaatcacc 32940

cataatgaca catcaaatta cgtaagagta ttaagagtat atgacagttg taacaaaaca 33000

actgcattga gaactagaag aacagacagc cacatgaacc atattcactt cttcagatca 33060

gaaagcctgg tgaactacca gtctaccaat tgctaccgta gtctccattt ttcacttata 33120

tgtacatagg acacctgcgc tatcagaata gccacaaagc cagttcactc gctacaaatt 33180

aatcttgact aaatccaaac agataccaac tcctcttaat caacttacaa aacacctaca 33240

cttttggtgt ctctacacca ccaatcacac aaaaggaaat taatcatcca cctactcgta 33300

tcctactgtt agaatggagg ccttacacca gagaatgggc attgccagat tctgagacac 33360

tgtcctttga gctccctgaa tctgaaccag taacaaaaat caaataagac acaacccaaa 33420

accttccgtt catcaagaag ttggatgttc aaattataaa gtgacagttc gatcgtacca 33480

ctggatgatg aggacccact gctcgcggcc tcagtatcct tctcaatctc cactgattgg 33540

taagttgctg ttggcatttc atccccgata tctacatact catccaactg ctcggcctgt 33600

gtagtgatct tgtcaggatc cttgctctcc tgtaaacaac aaaacacatt aaccattata 33660

agagctcatc tcacacataa gctaatagca accaagaaca aagcatgctg ctcactattg 33720

ccattgtcac atcaccgttg tccaccatcg tagcggtact gccattcacc agcatatcac 33780

tatcattagc cacggaagca tcaatgacat cggcattttc accattaaca atggcagccc 33840

gtcgactctt gttgagcgcc ttcttgaagt tgttgacgaa tcgatcaagc tcccactgtg 33900

tctcgacatc catctcatca atatccagct cgatctcacc cccaaccagc tcaggattgc 33960

cattcctctt ccgcacaatc tgcagcacat tatgcatctt ctcctctggc aagctctcca 34020

acccaaccct caacaagttc ttctcctcca gcgtcatatc cctcttgttc ggctcccttg 34080

ccttcggctt ccgcattttc acattcgctg cccttggttt cacctgcacc ggagctgtcg 34140

ccggtggcaa ttccggtggc ggcactggca tcggcggctc aaggagcttg agttcctgct 34200

cgaaccaaga catagatgcc ttgtacatct tctcaaagga cgccaggagg tcgccggcga 34260

aggtgtggac ctcgtgcccc gcagggttgt atctcagcgc gttgctgaac gtgagccgga 34320

cgtcggcggc gaagtcgtcg tgtgaggggt accttccggc ggcgaggttc gccctcaccg 34380

tgccgagatc catggggcac ttgatgactg cgtggtagtc gtggagcccg aggcggtcga 34440

cctcgacggg ggcgttgaac cagatgctcc gcttgtcctt ccgcagcttc gccaggatct 34500

gctcgcaacg cttccgcatc gccgccctga gcttcgccgg cggcgtgggg aggtcccggc 34560

gaggcgagcc atggcgcttc acctgaccct gctgccacgt gtcgatccgg gcgatgaggg 34620

cgcggacctg gccaagctcg ccggcgaggc ggtcccgcag cgcgcgggcc tcgcggtgag 34680

ccattgcccc cggccgcagc gccacgtagc ttgacggcgg cggcctcgtg gccgagccgt 34740

tggcgcgctg ctggagaggg tggttgggat cggaattggg ggccacgggc gcgagcgggg 34800

cacgggtctc cccccactgg tggtggtggt ggtggtggtt aaccccgccc cggccggcga 34860

gcagggcgga ggccatcacc gtcgccgtcg ccggcgaggg tttcgcgtgc tgctgctgct 34920

gcgctggcga gagtgggtgg atccgcacgg ggaaagggga gaagccgagg agatattttt 34980

tttctctctc tgtggcgggt ggatgtgccc gaggcgagat cctgaccgtt gatgtctatt 35040

cggaggcaga tcgaacggtg gagatctgcg tagagttgcc cgcgggcagt ggggagtgga 35100

cggctaggat gttggtaatg gtgtgacgtg gcaagatctt gctgcgtgat cgttatgacg 35160

tgattcgtga acatataacg gtagtgaagg tgcaactggg ttattatttg agtgattagt 35220

actagtttaa gtcttactac gtgattgtac atttgcactt agcttattac tccatctgtt 35280

ctatattact cgttgctttg attttttttc gtagtcaact ttttaaaata tgatagaaaa 35340

atatagcaac atttgaaaca caaaattagt ttgattaaat ctaatattgt atatattttg 35400

atgatatgtt taattggtgt tgaaaatgct actatttttt ctctaaactt agttaaatat 35460

aaagaaattt gattatgaaa aagaaataaa aaaacttata acataaaatg gggtcgtatt 35520

tttttacaca cttagcttat tacattgtga tacaactgta tgcatgtgcc aatgctcccg 35580

tgtcttgctt tcgaatctct ctgtaaagcg tgcgcaccaa aagaaagcag cattatatgg 35640

agggagccac gccatgtgcc cctttgttct gcaagcatgg tttgaactgg tctcccaacc 35700

tcctgatcaa aaggcctaca tgtatgtagc catttttctt gcaacaatta tatatacaaa 35760

gtagagatca tagtaaaagt catcgatata tctaacatat agatacataa tataaaacga 35820

ttaccatttt attagctagc aagtacatag ctgccatgag cagggaggca ttagctacta 35880

cttccttttc tttctttctt tctttttttt ttttacgttg gttagctcaa aattatatat 35940

gggaaaggac gtagtagtat gacacgtttc gttcttcgtt taaaaccaca acgagagcat 36000

cactgtatat atatgcggat cgaatatatt tacgcgccga tctcggagca cgctgctgct 36060

tcggacgccg gagcggcggc gaggtggagc cgcttctcgc cgtggacgat ggcggcggag 36120

gcggcggagc tcccgacgac gacgacgacg caccgcttct ccttggcctc ggcgcggcgc 36180

accaggacca acgagctgca gctgctgccg accatcacat tgcgaggagg aggaggcggc 36240

gctggcgcca cggcggcggc ggccgcggcg acgccacgga gcagcgcgta gctcgccgac 36300

agctcgcgcg ccatgactat ggccggcagc ggcggcgcgt cgggctcctc ctccgctatc 36360

gtgtccagca tcgccgggaa cccgcggcgg cggcggggcc tgatgatcga cgacagcagc 36420

ggcggcgcga cctcgacgga gaaccgggcg acgagctggg ccttcgccat tgtcggcgtc 36480

agcgtcagcg tcaagcgtgc gctacgcaca gcgatcggca gctcaagaat ctgtggccat 36540

ggaagcttcg tgctaagctt actggaaatt aatcgtagtc tgggtgctcg tatttataga 36600

cccgtacact gtcaatttgg aattgcaatt taattttgag ccatgcaatt tgcaagtgga 36660

tggttctctc tggtgggaaa ctgacgagta gataaatttt ggaaagaaaa tactagaaat 36720

gagacgattt tggaggcagg gataaatgag ttgaaacttg aaagtgatca ggcgaccaga 36780

gacactatca acgtcactgt gataatatta atgtagtagt aatgcctaga gaagtgttgc 36840

tatactgtcc cctgataaat gaagaattgt aaatgatgaa tattcagcag ggagtttttt 36900

tgtcagaaaa tacattgaac cttcaggcgt ctaaagacag catgaacaaa aaaaatttac 36960

atacgaacct caaatgaaca atacaaaatt ttcagagcta agacagcgtg aacaaaaaaa 37020

aaattgagag tgagaacata tgcataaatc attcatatag catccaacac gtactgatgc 37080

atcatctctc tcattacgcg acatacagta cagtatacaa tataaatgtt gttttgtaag 37140

cattctaatg aagaaaaaaa tctcggtacg catcgccaat aagaatttca gcaaaagaaa 37200

aacgatatat ttagtaaaga ctactgtcaa tttcatccaa ctttttgtag aagattgtag 37260

cgaggtttcc ccatggagga cggcaatcac atgaggcgga taagatcgga ggagttcctt 37320

tcatctcctt ttaggaaatt taacttccag gggcgctagg ctgtccctgt acaagccgtc 37380

ctctgcttgc tcagccagca acttggcatt cttcgtacag taccatggtg cacgcatggg 37440

ttatgaaaat gaaacgaaag tttttttaaa aaaaaacaaa tattttgaag ccaatattat 37500

agttataaga agtatttgtt gccaagattg aagctatgta aaactgtgat gatcaatagg 37560

acaataggtg tgagtgcacc tgtgatattg gtataggccc atttgtcaat gtgtgttaca 37620

agattattcg acagtaatag ttttcatgta tgggttgtca gttccatttc catgtccatg 37680

tcgaaatgaa atatctggta attgaaacat cattatcaaa tgtttatttt tagagatcaa 37740

attgcagtaa tttgccggaa cggagctgat gatatatgat cacaggtttt ttttcttttt 37800

ggcatttcat ctctcttctc atccacatga acccagtgag taactgttgc tccacaatca 37860

gtgagcagtg aatgtcaatg ccaagaacag tagtatatca agattcagtg aacaacatcc 37920

cgagagtact tacattttgg atgtcgtttg gtgccaattg gcagcattca gaataatgtg 37980

cagagaatct catgcccatc atcttacgtt tcagaacagt agatgaaaat tcgcaagcca 38040

cagaatgatg acatggaagg ccagtaggag cacagtaaat taaaatcagc acaatccatt 38100

gctccccttc tctgagcttg gttaaaggat tggagatcag tggtgctgat ctatcggtcg 38160

aaatcagtgt agctgttact ggaagccctt caccctctga aacacgctgt gagctttgtg 38220

accaggtagg ttcagccgtt caggggatcc aaattccaat ggcatcaatc aacatcgcgc 38280

tctacgtcaa tttcgagccg gtgcttgccc tttgcaggca gaaatataca gttgaacaaa 38340

tactgtacta cccctagcat gttcctttgc aaaccaccac tttctgaagt catacttgta 38400

tcatattcat atgcctaacc ttggcgtatc cctcttatgc tgttatgcaa ataggatggc 38460

ataagcttca tggagatgta accaggcatg caaattctaa ctcgaccaag atgacaggag 38520

gcggctgcag tatttgggca ataaaaatgg cagtttcaag tttcaaccat gttctgcagc 38580

tgagggcttt ggtggtgttt tcttttcctt taagtatctt taaaagggat acaagtagta 38640

accaccagtt aatgaaatcc cagcaattat gtcaaaaaaa attgaaatcc cagcaaaact 38700

aggctctttt ggctctagtt cctaccgggc atgctcccgt agaaaacaac attacgtcaa 38760

acttgggggc aaagcactca ttcattttca atatcgatcg tttaaattaa ttgttcgtca 38820

cacatacacc atcttgttca gtaatgatgg ctcacattgt ccgtaatccc tgcttaacta 38880

catattgact ccctcatttt atactcaaca atacacacca gactcccaca ctccattttc 38940

acagtgactt tatttagcgc accgcaagct gcaccagcac actcctcatg ccaaggagaa 39000

gcaaataaga taattgctcc aatgctgcct tcctcgagca atcaggggaa tggcttagtg 39060

cgctcgccgg gtgccaagtg ccaaatggcc ttagaagact ataatttttg tgcagctcct 39120

ggtctgttca cagccgggag atgagcagtg ggactgagac agtggcggtc tgctccccca 39180

ttgcagggtg gcacatctcc agcatgtcca gctccctgaa ccgctcaaga accctcattc 39240

ggcactcctc agctgccatc agaccagtgg agaaggcgcc atgcaccgtg ccagtgtact 39300

ggacactggt ggcttctcca gcaaagaata ggttatcgac tgggatccgc agcttctcat 39360

acaggtcacg aggtttgccc accccatcga aggtgtagga tccaagtgta ttctcgtctg 39420

agccccaatg tgacactagg taatgtatct gcaataagat ttgggcagca tggttgtaag 39480

aactagcata aagagattct tgattgccaa atttgatatt tttgtcaaga tactaaccgg 39540

ctcggcagcg ttgggcagga tcttcttcag ctgagagaag gcaaattggg cagcagcctc 39600

atctgacagc ttttcaatgt cacatgcaag ccgacccgca ggcatgtaaa ctagaacagg 39660

atggccagta gccttgtgga ggttgaggaa atagctgcag ccatatgtgg tggatgaaac 39720

tactccaagg aactccacat taggccagaa aacctcgctg aagtggagaa ttattttgtt 39780

ctcgactcca actgagagtt ctcttattgc ttcctccttc cactctggca gcctaggttc 39840

aaatttaatg gtgtttgctt taagaacacc taaggggaca gcaatgactg cagcatccgc 39900

aacaaatgtt ttaccactgc ttacagtaac ctccacccta ttcctgtggc gaacaatctc 39960

aacaacccta aagaaaagaa aataatgtgg attagcaata aggaatggtg gatggaacca 40020

tgaattttgc ttttaaagtg agaaggaaaa actaaatgga tatgcatacc tgtggccaag 40080

gcgtatatct aggccttttg ccagagtatt tattactgga cgatatccac gaaccatgag 40140

accatggcca ccaggaagca gtacctcctg cacccacagc acagtacgaa catgagacat 40200

agcaatcaaa ctagcatgag tacatcatga gcaaaatatt ggcaattgta gctgatatca 40260

ggaaccaaag tcctacctgg tcccaaccct gaagagagat tgcgtccgca tcagtagcaa 40320

accaaccttc catgcggcac aaataccact gaagaacatc atgagcaatc ccttcttgcc 40380

tgttgtgaac aaggacaggt caggcaaaat gaccaaaaga tcaggataac ctatgcacat 40440

gggcagaaat tggatggtac aaacctcaag tgtggatttc tctccataac aattgcaatg 40500

gccttcgcaa tagaaatgtc ttccttagtt tcttccctca gtttgccagt ctgcacatat 40560

aaatgttagg gaaaaacaag taccaaaaat attatcatgc aacaacaata agcatggaaa 40620

ctgaaacaag gtaatacctc ttccaatata gtctcaaaaa ccttccctat cttttctacc 40680

agctcttggg gaacttgatg gcccttagtg tcatagagag cataactgag ggaaaaaaca 40740

tgttaaatgg ttgattatta tggtccatca tctctcaagt tacatacaaa ataaactagc 40800

tctagtaacc tctcaaggtc atgatcaaac agcacagagt catctccact tgtgcgatac 40860

agtggaagtc caagccttcc aataattggt gccaggggat tttcctcaca aacaccatga 40920

agcctgatgc tcacacaaaa tggaagtgga ttaaaaataa tggttccaga taaacttttg 40980

ccaccatttc aaataattca gaaaatattg tcagagagga acctcaccag gatgctccca 41040

gatcaacagg aaagccaaaa gagtaatcag tgtgaattct accacctatc ctgtcacgag 41100

attctagaag aacaacctgg aaagaaacca cattagaata accagtgggg aagaatttca 41160

caacatttga aatgcacttc aagggatcat tgaacctata tcattacaat taggccataa 41220

actgcatcac atattacctc aaatgatgca ttcctgagag cgttggctgc agcaatgcct 41280

gcaaacccac tgccgataac aatagcagaa ggtgtgtggg actttctcct aacattttca 41340

ccatatgaac ctggatttta caaaaaaaaa aggaaaaaaa aaaggaacct caatgagagt 41400

gacaaagtgt agtaataaaa agttaaaaca ttataaaata ataatgatgt gtcaattttt 41460

ggttattatc ttgccaaaat attgcattga aaaaggagct cattcagctt aaatctaaag 41520

tgcaaatact ggctggcaca tgtaccatca tatgttcttt atatgactgt caacacagga 41580

agcaacctga aagtttcaca tgtttgatgt agttggatgg atgcttgtgt ttcatgaatc 41640

taaaggtttt gcaccttaag gctaacggtt tgcttcattg tgaaaggggg ttttatacct 41700

ccaaatgata aaaatcagtt tatacagaat taacataaag aagcctacca agtgatgaaa 41760

ttgccgatat cgcctgttct tctcacatca accctcctct accccctcca tcatggaggg 41820

ttagtagtga ggcatgttcc cctgtctcag aacaatgatg gtgaggtaag acaggacaat 41880

tttaacattc tgttgattcc ccacagaaac ctaaccgctt ggtctgtagc tacacattgg 41940

aagctgagat gtaggactga tcaaattttt cgctgggcag gcacaccaaa gactctccgt 42000

taaaacacaa tgacctcaaa ggatgtgcaa aaggcaagga cacaatatta ttattgtact 42060

gttctttcta gttatgtgga catggtgcaa attcagacta atgggcgata gacatgacat 42120

gaatttgtgg ctatatactg tttatcttgg aattatttgg tggcatacta taaattagac 42180

aatcttttag cgagacaacc ctctgccgga tatgcttcag agcacttaac aagcaaaaaa 42240

taatggccac cgaacatctc acacaatgac tataggcgca ctaaaaggca caatccaaaa 42300

gtaaagatat ccataaatga gacagcaacg aaagccaaca gaaaaaacat tttataagag 42360

ctgaacagta aaataagaaa atggtggcat tggaagataa agaaacttag ataactagat 42420

ccaccagcga atattctcat ataataatct gcaccctaca tgcattacaa tactatatga 42480

atcatgcatg catagaatgt ccgcatggca ctaaacttaa catgacatgc acttttgctg 42540

atctaatttt agaatagtaa gtatatgata tttaaagttc aagcactact gcatctgcat 42600

gttgttagac ctcaactgca accagtatgt gagtgcttgc atgaacggtt tacactagag 42660

tcttacaaat tgcaagctct tcaggaacta aaaggcgcag caggagccta caactccaca 42720

tggaggactt tctctcgcca tgagaaaaac cctagaaggt tccaattggt tgaggcgctg 42780

gtacaacagg tgtaaacagc taatgttttg ttttctcttc ctaccaccga cagaggaata 42840

tgggaactca tccacgatgg ctgatgtctc tttcatctgg cccactaggc acctcccaac 42900

aaccataagc aactcagtgt gtttagatta tcaaagcaat tttatcacta caaccaccta 42960

gagagtggaa aatttcggtt tttgcactaa ggttgcatta agtgcacaaa tccctaaaac 43020

aatacatgtt cgatattcta ctactagcag aggtattagc atacatcagc aaactttata 43080

aaacagtttc ttctaaattc gattttccac ctaactttac attaacagat gcaaaaaaaa 43140

aaagtcttga actgagaggg ggactttata atggaataaa caaaataaca taaaaatcag 43200

caacagaatc accacaaaaa caatggatga gaaaatctgc caaacgaagc caggaactta 43260

ctgttgttcg ccattgttgg ggttctgttt ctctccttca cgattactac tcgaattcga 43320

attacaattg gatggattta acagaaactg cggggagaaa tgcagcagga agatgatgag 43380

gcaggccacg tagtggttcg agttcgagtt gagtcccagg ttcacgaaac agttcatgca 43440

gtcgtgcagc caaatcgccg caacctccta gcaatttgat gtccaaccct ttcacgatct 43500

cacgtcagcg ccgagaattc gcgcgatccc atcaacttct caggcaatcc ggtttaagtc 43560

tggatcaact cccgcgtgat atcactgcga aaaaatcaaa ggaaaccaac cgtttattat 43620

tcacccaaat ttataaacct tcagttttag tcacacaaaa accgcttccc agcacacgaa 43680

atccgccgca aaatcgagag atttaagcga ttcaagaaag ccgatgatta aaactagacc 43740

aaaaatcaac cccctaagtc atcaggaagc caatctccga atcccacacc agaagcgatc 43800

gaatcgagcc aaggcaatca acaaacctag ggctccctat ctttcccctc gcaaacccag 43860

tccaaaaatc gcacgcaagc tatacgaaat cgcgccacac acccacctcg agatttcgat 43920

tcgaggcgca cgcagtcgat cggagcaaga gcaaaaaaaa acccgaagca gtcttgcggc 43980

gatcgatcga tcgatggatc ggatggtttt cgctggattt ttggagattt tcggagaggg 44040

ggatttggtt ttggggagag gaggagaacg agaccttccg ctttcctggc gtttcgggag 44100

ctttttgggc ttttggaggt gacgggatcc tgtcgagttg gggggggggg gggggggggg 44160

atttatagct tccttttccc gggggttacg tgcaccacca gggtcagctt accggctacg 44220

tgggggccac cccaccaaac ccgacatatc ttcaccgttg atctggattg gacggtccgg 44280

atcgacgcca cgtcgcccga aacgttggtg accgcacatc tgacgatatt ttcagcgtac 44340

atttacaccc tactatgaaa tacacaaccg taaatcgtac ataatcatcg tgtacaatag 44400

ggtctactaa gtaattttta agattgtaca ccctccgttt cacaatgtaa gtcattcaac 44460

ggagggagta ctctagtact gttttgtctt aaattatttt ttttcttttt atatttaatt 44520

aaagtataaa cggacgcaca cgatacatac acttcacttt ttactcactc caccacacac 44580

ccacacacgc atgcacgtct ttttatagcc aggctagaac tcgtaacagt attgtattat 44640

gcaaaaactg aacaatttca cgtgaaacat ctgcaaatct cacgggaagc cctcgagttt 44700

ataaaaaagt ttcataccga ataataatgt aatgtcatct gtgctcatcc aaggtcattt 44760

gtgtgataca ataacaccca atggctccac tagatggtac tacaattgga ggagcacaaa 44820

ctgcacagat gagacatgtc ctgcacagat gagacatgtc attggacgtt ggaatttaac 44880

taggtatatc ttcctccaat tgtcccatga ttaattttaa aaatacttaa tacctttcgt 44940

cttccatggt gagttcattg atgatgcaac acacaatcag aggacggtcg ttttattttc 45000

ttgacctggt aaaggtgcat ttataggtaa cacgtttttt taatcctctc cttaactata 45060

ccccttttgc atatgagagc cacttgcaca tttatctaac aaggagcaac tcgaaaaatc 45120

aaaatcaaaa ggaacttaat tcaccactct gactcattcc attgacacgt caccagttgc 45180

atttccacag cttatcattg catgcaacac aaaaattggg aggaaaatta tcacaaggag 45240

gaccatctct tctccgttct cacaaaccat gcattagcac cgtttcacag agaagagatc 45300

cccttccgaa aaaaacaaac tttagtttat ctgatatttc aaaaagttat ataatacgta 45360

gtactaactg gcatgtatat cttgaaaccc gtcgatccaa aaagaattct caaatcgtag 45420

gaagaacata ttcactggaa aggttgatat cgactggagc aaacagcatc acgatcacga 45480

ctggagtaca gtaacaagat ggcaaattaa tgattgtcta attgacgtac tagtacagtt 45540

ttttttttta tttttcgtga acgcgtaaaa actgttcaag tcactgtgga tatgtttgga 45600

agatcataaa attttaaaaa gctaccggta aggatatgaa aaggttaagt tcattttcta 45660

aattatataa ctatatattc tcaaaattta aataaaaatc tagacggttg agaaagcttc 45720

gcaactgaaa aattttaaaa gaagctaccg gctaccggcc ctaccgctgt cagaagttgt 45780

ccaaacagac ctatatagtt tcgtgcccgt ttatcattgg accgcctatg aacccactgc 45840

tagcctcgtg ctcgtagatt ggcgacaact agctacgcat gcaaaaacga tttgagaagg 45900

accgagaaag acgtgcgtga tggggccaga taccgtggcc tggcctggcc taaccggggc 45960

catcatttca tccaacgcac agaccattag gctctcgcta tccgaaagaa cagaaaatta 46020

aaacaaattc gccaccacaa acgatcgcgt cgctgcatct gctctctatc tgtacacgga 46080

gataagacac gagggaatta tgcacaaaaa agtctctctc tctctctctc tctctctctc 46140

tctctctctc tcattttcgg aggacgacgt taatctcgtt aattaattca gaacacgtaa 46200

tattcagaat atcaccagtt gatattatca cataatttag acttttaaca gagacgtgat 46260

ctacagtgca ttattcagag gatgcggcaa atatagagat gatcattata tcgttgaaac 46320

ggatgagcgc gcgtagttaa tctgtcaacg aagagctcgt tagatgctgc gaaaatgtag 46380

gctataccac gcgttaacta gccgaggcca gacgtttgta cgccaaggta atgatttcgc 46440

agcatctaac gagctcttcg gtcgcagtca taacttcgta tagcatacat tgtcggtcgc 46500

agtcataact tcgtatagca tacattgtcg agcttggcac tggccgtcgt tttacaacgt 46560

cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 46620

gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 46680

ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag 46740

tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt 46800

tattagaata acggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat 46860

gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct 46920

gctatagtgc agtcggcttc tgacgttcag tgcaggagat gatcgcggcc gggtacgtgt 46980

tcgagccgcc cgcgcatgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg 47040

atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc 47100

taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga 47160

tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa 47220

ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact 47280

cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc 47340

ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg 47400

cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc 47460

ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag 47520

cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt 47580

cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat 47640

cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat 47700

cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga 47760

acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact 47820

catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc 47880

cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc 47940

atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg 48000

gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag 48060

taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga 48120

aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg 48180

ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa 48240

gcccgaggaa tcggcgtgac ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc 48300

tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg 48360

aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat 48420

cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc 48480

aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca 48540

tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct 48600

acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt 48660

gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc 48720

gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca 48780

agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc 48840

ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg 48900

tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg 48960

ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag 49020

gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg 49080

gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt 49140

tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg 49200

tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc 49260

aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg 49320

gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa 49380

aagatctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc 49440

ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga 49500

ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa 49560

ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagctcccg 49620

gatacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg 49680

tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga 49740

gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc 49800

ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgt tcatccgctt 49860

cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 49920

caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 49980

caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 50040

ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 50100

cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 50160

ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 50220

tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 50280

gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 50340

ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 50400

ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 50460

gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 50520

aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 50580

tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 50640

ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatt 50700

ctagggaagg tgcgaacaag tccctgatat gagatcatgt ttgtcatctg gagccataga 50760

acagggttca tcatgagtca tcaacttacc ttcgccgaca gtgaattcag cagtaagcgc 50820

cgtcagacca gaaaagagat tttcttgtcc cgcatggagc agattctgcc atggcaaaac 50880

atggtggaag tcatcgagcc gttttacccc aaggctggta atggccggcg accttatccg 50940

ctggaaacca tgctacgcat tcactgcatg cagcattggt acaacctgag cgatggcgcg 51000

atggaagatg ctctgtacga aatcgcctcc atgcgtctgt ttgcccggtt atccctggat 51060

agcgccttgc cggaccgcac caccatcatg aatttccgcc acctgctgga acagcatcaa 51120

ctggcccgcc aattgttcaa gaccatcaat cgctggctgg ccgaagcagg cgtcatgatg 51180

actcaaggca ccttggtcga tgccaccatc attgaggcac ccagctcgac caagaacaaa 51240

gagcagcaac gcgatccgga gatgcatcag accaagaaag gcaatcagtg gcactttggc 51300

atgaaggccc acattggtgt cgatgccaag agtggcctga cccacagcct ggtcaccacc 51360

gcggccaacg agcatgacct caatcagctg ggtaatctgc tgcatggaga ggagcaattt 51420

gtctcagccg atgccggcta ccaaggggcg ccacagcgcg aggagctggc cgaggtggat 51480

gtggactggc tgatcgccga gcgccccggc aaggtaagaa ccttgaaaca gcatccacgc 51540

aagaacaaaa cggccatcaa catcgaatac atgaaagcca gcatccgggc cagggtggag 51600

cacccatttc gcatcatcaa gcgacagttc ggcttcgtga aagccagata caaggggttg 51660

ctgaaaaacg ataaccaact ggcgatgtta ttcacgctgg ccaacctgtt tcgggcggac 51720

caaatgatac gtcagtggga gagatctcac taaaaactgg ggataacgcc ttaaatggcg 51780

aagaaacggt ctaaataggc tgattcaagg catttacggg agaaaaaatc ggctcaaaca 51840

tgaagaaatg aaatgactga gtcagccgag aagaatttcc ccgcttattc gcaccttccc 51900

taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt 51960

gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat 52020

cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc 52080

aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg 52140

ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg 52200

atctttaaat ggagtgtcct cttcccagtt ttcgcaatcc acatcggcca gatcgttatt 52260

cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga 52320

tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg 52380

gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag 52440

attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc 52500

cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct 52560

gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg 52620

agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg 52680

tgatattctc attttagcca tttattattt ccttcctctt ttctacagta tttaaagata 52740

ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac 52800

cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta 52860

tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg tcatcgttac 52920

aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg 52980

ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc 53040

tgacgccgt 53049

Claims (7)

1.一种用于大片段DNA无缝组装的系统，其特征在于，包括由接受载体A、供给载体D1和供给载体D2组成的系统；

所述接受载体A的多克隆位点中含有一组核酸内切酶识别靶位点EN1，且按照：“载体骨架---反向EN1靶位点---任意碱基---同源臂13R---正向EN1靶位点---载体骨架”排列，所述接受载体A的载体骨架中不含有EN1靶位点；

所述供给载体D1的多克隆位点中含有三组核酸内切酶识别靶位点，且从上游到下游按照：“载体骨架---正向EN1靶位点---反向EN3靶位点---任意碱基---正向EN3靶位点---反向EN2靶位点---任意碱基---正向EN2靶位点---同源臂13R---反向EN1靶位点---载体骨架”排列，所述供给载体D1的载体骨架中不含有EN1、EN2、EN3靶位点；

所述供给载体D2的多克隆位点中含有三组核酸内切酶识别靶位点，且从上游到下游按照：“载体骨架---正向EN2靶位点---反向EN3靶位点---任意碱基---正向EN3靶位点---反向EN1靶位点---任意碱基---正向EN1靶位点---同源臂13R---反向EN2靶位点---载体骨架”排列，所述供给载体D2的载体骨架中不含有EN1、EN2、EN3靶位点；

所述核酸内切酶为识别位点和酶切位点不完全重复的DNA内切酶；

所述核酸内切酶为识别靶位点长度大于等于12bp的RNA介导的LbCas12a核酸内切酶；

所述EN1、EN2和EN3靶位点中含有限制性内切酶的酶切位点，所述EN1、EN2和EN3中限制性内切酶的酶切位点不同，且所述载体骨架不含有EN1、EN2、EN3靶位点以及该限制性内切酶的酶切位点。

2.如权利要求1所述的用于大片段DNA无缝组装的系统，其特征在于，所述核酸内切酶的正向识别靶位点的酶切位点在识别靶位点的下游，反向识别靶位点的酶切位点在识别靶位点的上游。

3.如权利要求1所述的用于大片段DNA无缝组装的系统，其特征在于，所述供给载体D1和供给载体D2的反向EN3靶位点和正向EN3靶位点之间添加负筛选基因或颜色显示基因。

4.如权利要求1所述的用于大片段DNA无缝组装的系统，其特征在于，所述接受载体A的反向EN1靶位点和正向EN1靶位点之间、所述供给载体D1的反向EN2靶位点和正向EN2靶位点之间以及所述供给载体D2的反向EN1靶位点和正向EN1靶位点之间添加颜色显示基因。

5.一种采用权利要求1～4任一项所述的用于大片段DNA无缝组装的系统的组装方法，其特征在于，包括以下步骤：

S1、构建载体系统；构建包括接受载体A和供给载体D1、供给载体D2的载体系统；所述接受载体A为pBWRA；

S2、目标片段克隆：将大片段DNA分成多个目标片段，并将目标片段按顺序依次交替克隆到供给载体D1和供给载体D2上，得到供给载体D1-seg1、D2-seg2、D1-seg3、D2-seg4…D1-segn-1、D2-segn等，n＝1,2,3,4,5,6，······等自然数；

S3、连续组装：用核酸内切酶EN1同时酶切接受载体A和酶切供给载体D1-seg1，通过组装反应将第一个目标片段装配到接受载体A中，得到pBWRA-seg1，然后采用核酸内切酶EN2同时酶切组装有第一个片段的pBWRA-seg1和酶切供给载体D2-seg2，通过组装反应将第二个片段装配到pBWRA-seg1中，得到pBWRA-seg1-seg2；

S4、重复步骤S3，根据确定的组装顺序将目标片段分步组装连接到接受载体A上，形成最终所需要的载体。

6.如权利要求5所述的组装方法，其特征在于，组装方式为同源重组，所述同源重组为任何一种对具体序列不依赖的同源重组方法。

7.权利要求5所述的组装方法在合成生物学、大片段DNA克隆或多基因载体构建中的应用。

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