JP7106625B2 - ゲノム大断片のダイレクトクローニングおよびｄｎａマルチ分子構築の新手法 - Google Patents

ゲノム大断片のダイレクトクローニングおよびｄｎａマルチ分子構築の新手法 Download PDF

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JP7106625B2
JP7106625B2 JP2020500937A JP2020500937A JP7106625B2 JP 7106625 B2 JP7106625 B2 JP 7106625B2 JP 2020500937 A JP2020500937 A JP 2020500937A JP 2020500937 A JP2020500937 A JP 2020500937A JP 7106625 B2 JP7106625 B2 JP 7106625B2
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exonuclease
dna
nucleic acid
fragments
acid molecules
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ヂャン，イォウミン
ワン，ハイロン
フゥー，ジュン
フランシス スチュアート，アドリアン
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シャントン ユニバーシティ
テクニシェ ウニヴェルジテート ドレスデン
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JP2020500937A 2017-03-23 2017-08-02 ゲノム大断片のダイレクトクローニングおよびｄｎａマルチ分子構築の新手法 Expired - Fee Related JP7106625B2 (ja)

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Application Number Priority Date Filing Date Title
CN201710177676.1 2017-03-23
CN201710177676.1A CN106995813B (zh) 2017-03-23 2017-03-23 基因组大片段直接克隆和dna多分子组装新技术
PCT/CN2017/000483 WO2018170614A1 (zh) 2017-03-23 2017-08-02 基因组大片段直接克隆和dna多分子组装新技术

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JP2020519304A JP2020519304A (ja) 2020-07-02
JP2020519304A5 JP2020519304A5 (https=) 2021-09-24
JP7106625B2 true JP7106625B2 (ja) 2022-07-26

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KR (1) KR102488128B1 (https=)
CN (1) CN106995813B (https=)
CA (1) CA3060585A1 (https=)
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CN106995813B (zh) * 2017-03-23 2020-06-16 山东大学 基因组大片段直接克隆和dna多分子组装新技术
KR20210152597A (ko) 2017-12-22 2021-12-15 (주)지플러스생명과학 키메라 게놈 조작 분자 및 방법
CN108676847A (zh) * 2017-12-30 2018-10-19 深圳人体密码基因科技有限公司 大片段基因组的捕获探针的制备方法及试剂盒
CN111088275B (zh) * 2018-10-23 2023-06-27 黄菁 Dna大片段的克隆方法
CA3129869A1 (en) * 2019-03-08 2020-09-17 Zymergen Inc. Pooled genome editing in microbes
CN110846239B (zh) * 2019-11-29 2022-08-23 南京工业大学 具有高同源重组效率的重组解脂耶氏酵母菌及其构建方法和应用
CN112852849B (zh) * 2019-12-31 2023-03-14 湖北伯远合成生物科技有限公司 一种用于大片段dna无缝组装的系统及组装方法
IL294909A (en) * 2020-02-13 2022-09-01 Zymergen Inc Metagenomic library and natural product discovery platform
CN113355339B (zh) * 2020-03-05 2023-01-24 山东大学 一种大型基因簇的无痕定点改造方法及其应用
CN111440753A (zh) * 2020-03-16 2020-07-24 扬州大学 禽致病性大肠杆菌clbH基因缺失株及其构建方法和应用
CN116135982A (zh) * 2021-11-16 2023-05-19 北京光华生明生物科技有限公司 用于基因片段连接的应用方法和试剂盒
WO2023178544A1 (zh) * 2022-03-23 2023-09-28 深圳华大生命科学研究院 表达元件组合、表达载体、宿主、应用及组装方法
CN115960733B (zh) * 2022-09-19 2023-11-24 苏州泓迅生物科技股份有限公司 一种用于大片段dna组装的基因工程酵母菌、构建方法及其应用
CN115948445A (zh) * 2022-12-12 2023-04-11 河南科技大学 谷氨酸棒杆菌CRISPR-Cpf1基因编辑系统、构建方法及应用
CN116355931A (zh) * 2023-05-29 2023-06-30 北京因诺惟康医药科技有限公司 一种快速分子克隆方法

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JP2004535773A (ja) 2001-02-09 2004-12-02 ジーン ブリッジス ゲゼルシャフト ミット ベシュレンクテル ハフツング 組換え法
US20070037197A1 (en) 2005-08-11 2007-02-15 Lei Young In vitro recombination method
US20070037196A1 (en) 2005-08-11 2007-02-15 The J. Craig Venter Institute, Inc. Method for in vitro recombination
JP2013530689A (ja) 2010-06-10 2013-08-01 ジーン ブリッジス ゲゼルシャフト ミット ベシュレンクテル ハフツング 直接クローニング
US20150072381A1 (en) 2012-03-30 2015-03-12 Shenzhen Zhonglian Biotechnology Co., Ltd Homologous recombination-based nucleic acid molecular cloning method and related kit
WO2016033315A2 (en) 2014-08-27 2016-03-03 New England Biolabs, Inc. Synthon formation

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JP2004535773A (ja) 2001-02-09 2004-12-02 ジーン ブリッジス ゲゼルシャフト ミット ベシュレンクテル ハフツング 組換え法
US20070037197A1 (en) 2005-08-11 2007-02-15 Lei Young In vitro recombination method
US20070037196A1 (en) 2005-08-11 2007-02-15 The J. Craig Venter Institute, Inc. Method for in vitro recombination
JP2013530689A (ja) 2010-06-10 2013-08-01 ジーン ブリッジス ゲゼルシャフト ミット ベシュレンクテル ハフツング 直接クローニング
US20150072381A1 (en) 2012-03-30 2015-03-12 Shenzhen Zhonglian Biotechnology Co., Ltd Homologous recombination-based nucleic acid molecular cloning method and related kit
WO2016033315A2 (en) 2014-08-27 2016-03-03 New England Biolabs, Inc. Synthon formation

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DK3604524T3 (da) 2021-12-20
CN106995813B (zh) 2020-06-16
ES2902210T3 (es) 2022-03-25
CN106995813A (zh) 2017-08-01
KR102488128B1 (ko) 2023-01-12
JP2020519304A (ja) 2020-07-02
WO2018170614A1 (zh) 2018-09-27
WO2018170614A8 (zh) 2018-11-01
KR20190133200A (ko) 2019-12-02
EP3604524A1 (en) 2020-02-05
US20200010867A1 (en) 2020-01-09
CA3060585A1 (en) 2018-09-27
EP3604524A4 (en) 2020-04-29
EP3604524B1 (en) 2021-10-06

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