JP2020519265A5 - - Google Patents

Info

Publication number

JP2020519265A5

JP2020519265A5 JP2019561819A JP2019561819A JP2020519265A5 JP 2020519265 A5 JP2020519265 A5 JP 2020519265A5 JP 2019561819 A JP2019561819 A JP 2019561819A JP 2019561819 A JP2019561819 A JP 2019561819A JP 2020519265 A5 JP2020519265 A5 JP 2020519265A5

Authority

JP

Japan

Prior art keywords

antibody

cell culture

collection

during

hydrogen peroxide

Prior art date

2018-05-08

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
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• 238000000034 method Methods 0.000 claims description 14
• 238000004113 cell culture Methods 0.000 claims description 7
• 238000011177 media preparation Methods 0.000 claims description 7
• 238000003860 storage Methods 0.000 claims description 7
• 238000004519 manufacturing process Methods 0.000 claims description 6
• 239000006143 cell culture medium Substances 0.000 claims description 5
• RMRCNWBMXRMIRW-BYFNXCQMSA-M cyanocobalamin Chemical compound N#C[Co+]N([C@]1([H])[C@H](CC(N)=O)[C@]\2(CCC(=O)NC[C@H](C)OP(O)(=O)OC3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)C)C/2=C(C)\C([C@H](C/2(C)C)CCC(N)=O)=N\C\2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O RMRCNWBMXRMIRW-BYFNXCQMSA-M 0.000 claims description 5
• 238000006243 chemical reaction Methods 0.000 claims description 4
• FDJOLVPMNUYSCM-UVKKECPRSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2,7, Chemical compound [Co+3].N#[C-].C1([C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)[N-]\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O FDJOLVPMNUYSCM-UVKKECPRSA-L 0.000 claims description 4
• 230000016784 immunoglobulin production Effects 0.000 claims description 4
• 230000002401 inhibitory effect Effects 0.000 claims description 4
• 235000000639 cyanocobalamin Nutrition 0.000 claims description 3
• 239000011666 cyanocobalamin Substances 0.000 claims description 3
• 239000001963 growth medium Substances 0.000 claims description 2
• MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims 12
• YOZNUFWCRFCGIH-BYFNXCQMSA-L hydroxocobalamin Chemical compound O[Co+]N([C@]1([H])[C@H](CC(N)=O)[C@]\2(CCC(=O)NC[C@H](C)OP(O)(=O)OC3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)C)C/2=C(C)\C([C@H](C/2(C)C)CCC(N)=O)=N\C\2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O YOZNUFWCRFCGIH-BYFNXCQMSA-L 0.000 claims 4
• 150000002978 peroxides Chemical class 0.000 claims 4
• 238000012258 culturing Methods 0.000 claims 2
• 229960002104 cyanocobalamin Drugs 0.000 claims 2
• 235000004867 hydroxocobalamin Nutrition 0.000 claims 2
• 239000011704 hydroxocobalamin Substances 0.000 claims 2
• 229960001103 hydroxocobalamin Drugs 0.000 claims 2
• 239000002609 medium Substances 0.000 claims 2
• VTIIJXUACCWYHX-UHFFFAOYSA-L disodium;carboxylatooxy carbonate Chemical group [Na+].[Na+].[O-]C(=O)OOC([O-])=O VTIIJXUACCWYHX-UHFFFAOYSA-L 0.000 claims 1
• 150000001451 organic peroxides Chemical class 0.000 claims 1
• 102000004169 proteins and genes Human genes 0.000 claims 1
• 108090000623 proteins and genes Proteins 0.000 claims 1
• 229960001922 sodium perborate Drugs 0.000 claims 1
• 229940045872 sodium percarbonate Drugs 0.000 claims 1
• YKLJGMBLPUQQOI-UHFFFAOYSA-M sodium;oxidooxy(oxo)borane Chemical compound [Na+].[O-]OB=O YKLJGMBLPUQQOI-UHFFFAOYSA-M 0.000 claims 1
• 210000004027 cell Anatomy 0.000 description 2
• 241000699802 Cricetulus griseus Species 0.000 description 1
• 238000010364 biochemical engineering Methods 0.000 description 1
• 238000011217 control strategy Methods 0.000 description 1
• 238000011118 depth filtration Methods 0.000 description 1
• 238000005189 flocculation Methods 0.000 description 1
• 230000016615 flocculation Effects 0.000 description 1
• 239000011521 glass Substances 0.000 description 1
• 239000007788 liquid Substances 0.000 description 1
• 210000004962 mammalian cell Anatomy 0.000 description 1
• 239000000463 material Substances 0.000 description 1
• 210000001672 ovary Anatomy 0.000 description 1
• 238000002360 preparation method Methods 0.000 description 1
• 238000011084 recovery Methods 0.000 description 1
• 229910001220 stainless steel Inorganic materials 0.000 description 1
• 239000010935 stainless steel Substances 0.000 description 1
• 229940045999 vitamin b 12 Drugs 0.000 description 1