CN110831964A - 在抗体制造期间控制粉红色形成的方法 - Google Patents

在抗体制造期间控制粉红色形成的方法 Download PDF

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Publication number
CN110831964A
CN110831964A CN201880031241.9A CN201880031241A CN110831964A CN 110831964 A CN110831964 A CN 110831964A CN 201880031241 A CN201880031241 A CN 201880031241A CN 110831964 A CN110831964 A CN 110831964A
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CN
China
Prior art keywords
antibody
cbl
cell culture
harvest
hydrogen peroxide
Prior art date
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Pending
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CN201880031241.9A
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English (en)
Chinese (zh)
Inventor
杜诚
A·U·博尔万卡尔
余骏昌
谭志俊
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Bristol Myers Squibb Co
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Bristol Myers Squibb Co
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Publication date
Application filed by Bristol Myers Squibb Co filed Critical Bristol Myers Squibb Co
Publication of CN110831964A publication Critical patent/CN110831964A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C12N5/163—Animal cells one of the fusion partners being a B or a T lymphocyte
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201880031241.9A 2017-05-09 2018-05-08 在抗体制造期间控制粉红色形成的方法 Pending CN110831964A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762503615P 2017-05-09 2017-05-09
US62/503,615 2017-05-09
PCT/US2018/031542 WO2018208743A1 (en) 2017-05-09 2018-05-08 Method of controlling the formation of pink color during antibody manufacturing

Publications (1)

Publication Number Publication Date
CN110831964A true CN110831964A (zh) 2020-02-21

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CN201880031241.9A Pending CN110831964A (zh) 2017-05-09 2018-05-08 在抗体制造期间控制粉红色形成的方法

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US (1) US12241107B2 (enExample)
EP (1) EP3621989B1 (enExample)
JP (2) JP7453788B2 (enExample)
KR (1) KR102664829B1 (enExample)
CN (1) CN110831964A (enExample)
ES (1) ES2951287T3 (enExample)
WO (1) WO2018208743A1 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112022012467A2 (pt) 2020-01-31 2022-09-06 Chugai Pharmaceutical Co Ltd Método para produção de composição contendo polipeptídeo com coloração suprimida
JP2025529484A (ja) * 2022-09-15 2025-09-04 サノフイ タンパク質の還元感受性の決定方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006060083A1 (en) * 2004-10-22 2006-06-08 Amgen Inc. Methods for refolding polypeptides
US20090053786A1 (en) * 2007-07-09 2009-02-26 Yung-Hsiang Kao Prevention of disulfide bond reduction during recombinant production of polypeptides
US20170002393A1 (en) * 2013-12-04 2017-01-05 Immunogen, Inc. Compositions and methods for antibody production

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2689458B2 (ja) * 1988-02-09 1997-12-10 武田薬品工業株式会社 粒状ないし粉末状のビタミンｂ▲下１▼▲下２▼類含有組成物
JP2734709B2 (ja) * 1990-01-05 1998-04-02 東レ株式会社 インクジェット染色用布帛およびそれを用いてなるインクジェット染色法
SE525083C2 (sv) * 2003-04-23 2004-11-23 Kemira Kemi Ab Sätt att behandla rötslam
EP1971488A2 (en) * 2005-12-05 2008-09-24 Lifeline Cell Technology Cell culture medium container assembly
EP2453874A2 (en) 2009-07-14 2012-05-23 Biogen Idec MA Inc. Methods for inhibiting yellow color and peroxide formation in a composition

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006060083A1 (en) * 2004-10-22 2006-06-08 Amgen Inc. Methods for refolding polypeptides
US20090053786A1 (en) * 2007-07-09 2009-02-26 Yung-Hsiang Kao Prevention of disulfide bond reduction during recombinant production of polypeptides
US20170002393A1 (en) * 2013-12-04 2017-01-05 Immunogen, Inc. Compositions and methods for antibody production

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
GAYLE E DERFUS等: "Red colored IgG4 caused by vitamin B12 from cell culture media combined with disulfide reduction at harvest", 《MABS》 *
GAYLE E DERFUS等: "Red colored IgG4 caused by vitamin B12 from cell culture media combined with disulfide reduction at harvest", 《MABS》, vol. 6, no. 3, 19 February 2014 (2014-02-19), pages 679 - 688, XP002784132 *
KENNETH M PRENTICE等: "Hydroxocobalamin association during cell culture results in pink therapeutic proteins", 《MABS》 *
KENNETH M PRENTICE等: "Hydroxocobalamin association during cell culture results in pink therapeutic proteins", 《MABS》, vol. 5, no. 6, 26 July 2013 (2013-07-26), pages 974 - 981, XP002784134 *
M. V. SIDOROVA等: "The Use of Hydrogen Peroxide for Closing Disulfide Bridges in Peptides", 《RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY》 *
M. V. SIDOROVA等: "The Use of Hydrogen Peroxide for Closing Disulfide Bridges in Peptides", 《RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY》, vol. 30, no. 2, 31 March 2004 (2004-03-31), pages 101 - 110, XP002605163 *

Also Published As

Publication number Publication date
EP3621989A1 (en) 2020-03-18
EP3621989B1 (en) 2023-06-28
JP7453788B2 (ja) 2024-03-21
JP2020519265A (ja) 2020-07-02
US20230103511A1 (en) 2023-04-06
JP2023182593A (ja) 2023-12-26
WO2018208743A9 (en) 2019-12-05
KR20200004355A (ko) 2020-01-13
US12241107B2 (en) 2025-03-04
ES2951287T3 (es) 2023-10-19
KR102664829B1 (ko) 2024-05-09
WO2018208743A1 (en) 2018-11-15

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