JP2020507312A5 - - Google Patents
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- Publication number
- JP2020507312A5 JP2020507312A5 JP2019539756A JP2019539756A JP2020507312A5 JP 2020507312 A5 JP2020507312 A5 JP 2020507312A5 JP 2019539756 A JP2019539756 A JP 2019539756A JP 2019539756 A JP2019539756 A JP 2019539756A JP 2020507312 A5 JP2020507312 A5 JP 2020507312A5
- Authority
- JP
- Japan
- Prior art keywords
- dna
- crispr
- ctag
- digested
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762457493P | 2017-02-10 | 2017-02-10 | |
| US62/457,493 | 2017-02-10 | ||
| PCT/US2018/017573 WO2018148511A1 (en) | 2017-02-10 | 2018-02-09 | A modular universal plasmid design strategy for the assembly and editing of multiple dna constructs for multiple hosts |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2020507312A JP2020507312A (ja) | 2020-03-12 |
| JP2020507312A5 true JP2020507312A5 (https=) | 2021-03-25 |
Family
ID=63107838
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019539756A Withdrawn JP2020507312A (ja) | 2017-02-10 | 2018-02-09 | 複数の宿主用の複数のｄｎａコンストラクトのアセンブリ及び編集のためのモジュラーユニバーサルプラスミド設計戦略 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US11098305B2 (https=) |
| EP (1) | EP3580337A4 (https=) |
| JP (1) | JP2020507312A (https=) |
| KR (1) | KR20190116282A (https=) |
| CN (1) | CN110312797A (https=) |
| CA (1) | CA3049989A1 (https=) |
| WO (1) | WO2018148511A1 (https=) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3580337A4 (en) | 2017-02-10 | 2020-12-02 | Zymergen, Inc. | MODULAR UNIVERSAL PLASMID DESIGN STRATEGY FOR ASSEMBLY AND EDITING MULTIPLE MULTI-HOST DNA CONSTRUCTIONS |
| CA3107002A1 (en) | 2018-08-15 | 2020-04-30 | Zymergen Inc. | Applications of crispri in high throughput metabolic engineering |
| WO2020206134A1 (en) | 2019-04-04 | 2020-10-08 | Regeneron Pharmaceuticals, Inc. | Methods for scarless introduction of targeted modifications into targeting vectors |
| US12497609B2 (en) * | 2019-10-14 | 2025-12-16 | Yonsei University Biohealth Technology Holdings. Inc. | Protospacer adjacent motif sequence and method for modifying target nucleic acid in genome of cell by using same |
| KR102178813B1 (ko) | 2019-10-18 | 2020-11-13 | 주식회사 다우진유전자연구소 | Dna 구조체를 적용한 간이 키트 |
| CN115052980B (zh) * | 2019-11-18 | 2024-09-10 | 苏州齐禾生科生物科技有限公司 | 衍生自黄杆菌的基因编辑系统 |
| WO2021108324A1 (en) * | 2019-11-27 | 2021-06-03 | Technical University Of Denmark | Constructs, compositions and methods thereof having improved genome editing efficiency and specificity |
| AU2021230464A1 (en) | 2020-03-03 | 2022-09-01 | Codex Dna, Inc. | Methods for assembling nucleic acids |
| CN113373130B (zh) * | 2021-05-31 | 2023-12-22 | 复旦大学 | Cas12蛋白、含有Cas12蛋白的基因编辑系统及应用 |
| GB202117455D0 (en) * | 2021-12-02 | 2022-01-19 | Academisch Ziekenhuis Leiden | Method of editing nucleic acid |
| KR20240169697A (ko) * | 2022-04-08 | 2024-12-03 | 글락소스미스클라인 인털렉츄얼 프로퍼티 디벨로프먼트 리미티드 | 올리고뉴클레오티드를 포함한 폴리뉴클레오티드의 신규 생산 방법 |
| CN116284436B (zh) * | 2022-11-25 | 2026-04-07 | 广州美格生物科技有限公司 | 嵌合体TsCas12a蛋白及其制备方法和应用 |
| CN119432813A (zh) * | 2023-12-29 | 2025-02-14 | 武汉艾迪晶生物科技有限公司 | Cas蛋白、组合蛋白、核酸分子、核糖核蛋白复合物、重组载体、转基因细胞及其应用 |
| WO2025165780A1 (en) * | 2024-02-01 | 2025-08-07 | The Board Of Trustees Of The Leland Stanford Junior University | Combinatorial gene editing and screening |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0695305B1 (en) | 1993-04-12 | 2003-08-06 | Northwestern University | Method of forming oligonucleotides |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| ATE362986T1 (de) | 1998-10-30 | 2007-06-15 | Cornell Res Foundation Inc | High fidelity thermostabile ligase und ihre verwendung |
| KR20100133319A (ko) * | 2009-06-11 | 2010-12-21 | 주식회사 툴젠 | 표적 특이적인 게놈의 재배열을 위한 표적 특이적 뉴클레아제 및 이의 용도 |
| GB2481425A (en) * | 2010-06-23 | 2011-12-28 | Iti Scotland Ltd | Method and device for assembling polynucleic acid sequences |
| EP3241902B1 (en) | 2012-05-25 | 2018-02-28 | The Regents of The University of California | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
| AU2013359262C1 (en) | 2012-12-12 | 2021-05-13 | Massachusetts Institute Of Technology | CRISPR-Cas component systems, methods and compositions for sequence manipulation |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| WO2014093694A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Crispr-cas nickase systems, methods and compositions for sequence manipulation in eukaryotes |
| CN105121648B (zh) | 2012-12-12 | 2021-05-07 | 布罗德研究所有限公司 | 用于序列操纵的系统、方法和优化的指导组合物的工程化 |
| CA2894684A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Engineering and optimization of improved crispr-cas systems, methods and enzyme compositions for sequence manipulation in eukaryotes |
| DK2931898T3 (en) | 2012-12-12 | 2016-06-20 | Massachusetts Inst Technology | CONSTRUCTION AND OPTIMIZATION OF SYSTEMS, PROCEDURES AND COMPOSITIONS FOR SEQUENCE MANIPULATION WITH FUNCTIONAL DOMAINS |
| AU2014227653B2 (en) * | 2013-03-15 | 2017-04-20 | The General Hospital Corporation | Using RNA-guided foki nucleases (RFNs) to increase specificity for RNA-guided genome editing |
| US11414695B2 (en) | 2013-05-29 | 2022-08-16 | Agilent Technologies, Inc. | Nucleic acid enrichment using Cas9 |
| EP3004370B1 (en) | 2013-06-05 | 2024-08-21 | Duke University | Rna-guided gene editing and gene regulation |
| WO2014204724A1 (en) * | 2013-06-17 | 2014-12-24 | The Broad Institute Inc. | Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation |
| MX2015017312A (es) * | 2013-06-17 | 2017-04-10 | Broad Inst Inc | Suministro y uso de composiciones, vectores y sistemas crispr-cas para la modificación dirigida y terapia hepáticas. |
| US10287590B2 (en) | 2014-02-12 | 2019-05-14 | Dna2.0, Inc. | Methods for generating libraries with co-varying regions of polynuleotides for genome modification |
| HUE049405T2 (hu) * | 2014-06-23 | 2020-09-28 | Regeneron Pharma | Nukleáz-közvetített DNS-összeállítás |
| US20180002706A1 (en) * | 2014-12-30 | 2018-01-04 | University Of South Florida | Methods and compositions for cloning into large vectors |
| WO2016205759A1 (en) * | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation |
| EP4159856A1 (en) * | 2015-06-18 | 2023-04-05 | The Broad Institute, Inc. | Novel crispr enzymes and systems |
| US9790490B2 (en) * | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| US20190330659A1 (en) | 2016-07-15 | 2019-10-31 | Zymergen Inc. | Scarless dna assembly and genome editing using crispr/cpf1 and dna ligase |
| CN109563505A (zh) * | 2016-07-28 | 2019-04-02 | 帝斯曼知识产权资产管理有限公司 | 用于真核细胞的组装系统 |
| EP3580337A4 (en) | 2017-02-10 | 2020-12-02 | Zymergen, Inc. | MODULAR UNIVERSAL PLASMID DESIGN STRATEGY FOR ASSEMBLY AND EDITING MULTIPLE MULTI-HOST DNA CONSTRUCTIONS |
| CA3107002A1 (en) * | 2018-08-15 | 2020-04-30 | Zymergen Inc. | Applications of crispri in high throughput metabolic engineering |
-
2018
- 2018-02-09 EP EP18751852.7A patent/EP3580337A4/en not_active Withdrawn
- 2018-02-09 WO PCT/US2018/017573 patent/WO2018148511A1/en not_active Ceased
- 2018-02-09 KR KR1020197022014A patent/KR20190116282A/ko not_active Withdrawn
- 2018-02-09 CA CA3049989A patent/CA3049989A1/en active Pending
- 2018-02-09 CN CN201880012880.0A patent/CN110312797A/zh active Pending
- 2018-02-09 JP JP2019539756A patent/JP2020507312A/ja not_active Withdrawn
-
2019
- 2019-08-08 US US16/535,245 patent/US11098305B2/en not_active Expired - Fee Related
-
2021
- 2021-08-03 US US17/392,366 patent/US20210371856A1/en not_active Abandoned
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