JP2020507312A5 - - Google Patents
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- JP2020507312A5 JP2020507312A5 JP2019539756A JP2019539756A JP2020507312A5 JP 2020507312 A5 JP2020507312 A5 JP 2020507312A5 JP 2019539756 A JP2019539756 A JP 2019539756A JP 2019539756 A JP2019539756 A JP 2019539756A JP 2020507312 A5 JP2020507312 A5 JP 2020507312A5
- Authority
- JP
- Japan
- Prior art keywords
- dna
- crispr
- ctag
- digested
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762457493P | 2017-02-10 | 2017-02-10 | |
| US62/457,493 | 2017-02-10 | ||
| PCT/US2018/017573 WO2018148511A1 (en) | 2017-02-10 | 2018-02-09 | A modular universal plasmid design strategy for the assembly and editing of multiple dna constructs for multiple hosts |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2020507312A JP2020507312A (ja) | 2020-03-12 |
| JP2020507312A5 true JP2020507312A5 (https=) | 2021-03-25 |
Family
ID=63107838
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019539756A Withdrawn JP2020507312A (ja) | 2017-02-10 | 2018-02-09 | 複数の宿主用の複数のｄｎａコンストラクトのアセンブリ及び編集のためのモジュラーユニバーサルプラスミド設計戦略 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US11098305B2 (https=) |
| EP (1) | EP3580337A4 (https=) |
| JP (1) | JP2020507312A (https=) |
| KR (1) | KR20190116282A (https=) |
| CN (1) | CN110312797A (https=) |
| CA (1) | CA3049989A1 (https=) |
| WO (1) | WO2018148511A1 (https=) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2020507312A (ja) | 2017-02-10 | 2020-03-12 | ザイマージェン インコーポレイテッド | 複数の宿主用の複数のｄｎａコンストラクトのアセンブリ及び編集のためのモジュラーユニバーサルプラスミド設計戦略 |
| CA3107002A1 (en) * | 2018-08-15 | 2020-04-30 | Zymergen Inc. | Applications of crispri in high throughput metabolic engineering |
| RU2771374C1 (ru) | 2019-04-04 | 2022-05-04 | Редженерон Фармасьютикалс, Инк. | Способы для бесшовного внесения целевых модификаций в направленные векторы |
| WO2021075827A1 (ko) * | 2019-10-14 | 2021-04-22 | 연세대학교 산학협력단 | 신규 프로토스페이서 인접 모티프 서열 및 이를 이용한 세포의 유전체에서 표적 핵산을 변형시키는 방법 |
| KR102178813B1 (ko) | 2019-10-18 | 2020-11-13 | 주식회사 다우진유전자연구소 | Dna 구조체를 적용한 간이 키트 |
| EP4063500A4 (en) * | 2019-11-18 | 2023-12-27 | Suzhou Qi Biodesign biotechnology Company Limited | GENE EDITING SYSTEM DERIVED FROM BACTERIA OF THE GENUS FLAVOBACTERIUM |
| EP4065701A4 (en) * | 2019-11-27 | 2023-11-29 | Danmarks Tekniske Universitet | CONSTRUCTS, COMPOSITIONS AND METHODS THEREOF WITH IMPROVED GENOMEDITING EFFICIENCY AND SPECIFICITY |
| JP7742355B2 (ja) | 2020-03-03 | 2025-09-19 | コーデックス ディーエヌエー インコーポレイテッド | 核酸をアセンブルするための方法 |
| CN113373130B (zh) * | 2021-05-31 | 2023-12-22 | 复旦大学 | Cas12蛋白、含有Cas12蛋白的基因编辑系统及应用 |
| GB202117455D0 (en) * | 2021-12-02 | 2022-01-19 | Academisch Ziekenhuis Leiden | Method of editing nucleic acid |
| KR20240169697A (ko) * | 2022-04-08 | 2024-12-03 | 글락소스미스클라인 인털렉츄얼 프로퍼티 디벨로프먼트 리미티드 | 올리고뉴클레오티드를 포함한 폴리뉴클레오티드의 신규 생산 방법 |
| CN116284436B (zh) * | 2022-11-25 | 2026-04-07 | 广州美格生物科技有限公司 | 嵌合体TsCas12a蛋白及其制备方法和应用 |
| CN119432813A (zh) * | 2023-12-29 | 2025-02-14 | 武汉艾迪晶生物科技有限公司 | Cas蛋白、组合蛋白、核酸分子、核糖核蛋白复合物、重组载体、转基因细胞及其应用 |
| WO2025165780A1 (en) * | 2024-02-01 | 2025-08-07 | The Board Of Trustees Of The Leland Stanford Junior University | Combinatorial gene editing and screening |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2204913T3 (es) | 1993-04-12 | 2004-05-01 | Northwestern University | Metodo para formacion de oligonucleotidos. |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| EP1127135B1 (en) | 1998-10-30 | 2007-05-23 | Cornell Research Foundation, Inc. | High fidelity thermostable ligase and uses thereof |
| KR20100133319A (ko) * | 2009-06-11 | 2010-12-21 | 주식회사 툴젠 | 표적 특이적인 게놈의 재배열을 위한 표적 특이적 뉴클레아제 및 이의 용도 |
| GB2481425A (en) * | 2010-06-23 | 2011-12-28 | Iti Scotland Ltd | Method and device for assembling polynucleic acid sequences |
| AU2013266968B2 (en) | 2012-05-25 | 2017-06-29 | Emmanuelle CHARPENTIER | Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription |
| CN113355357B (zh) | 2012-12-12 | 2024-12-03 | 布罗德研究所有限公司 | 对用于序列操纵的改进的系统、方法和酶组合物进行的工程化和优化 |
| IL239344B2 (en) | 2012-12-12 | 2024-06-01 | Broad Inst Inc | Systems engineering, methods and optimal guiding components for sequence manipulation |
| WO2014093655A2 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| EP2840140B2 (en) | 2012-12-12 | 2023-02-22 | The Broad Institute, Inc. | Crispr-Cas based method for mutation of prokaryotic cells |
| US20140310830A1 (en) | 2012-12-12 | 2014-10-16 | Feng Zhang | CRISPR-Cas Nickase Systems, Methods And Compositions For Sequence Manipulation in Eukaryotes |
| CN112301024A (zh) * | 2013-03-15 | 2021-02-02 | 通用医疗公司 | 使用RNA引导的FokI核酸酶(RFN)提高RNA引导的基因组编辑的特异性 |
| US9873907B2 (en) | 2013-05-29 | 2018-01-23 | Agilent Technologies, Inc. | Method for fragmenting genomic DNA using CAS9 |
| WO2014197748A2 (en) | 2013-06-05 | 2014-12-11 | Duke University | Rna-guided gene editing and gene regulation |
| RU2716420C2 (ru) * | 2013-06-17 | 2020-03-11 | Те Брод Инститьют Инк. | Доставка и применение систем crispr-cas, векторов и композиций для целенаправленного воздействия и терапии в печени |
| WO2014204724A1 (en) * | 2013-06-17 | 2014-12-24 | The Broad Institute Inc. | Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation |
| US10287590B2 (en) | 2014-02-12 | 2019-05-14 | Dna2.0, Inc. | Methods for generating libraries with co-varying regions of polynuleotides for genome modification |
| ES2781323T3 (es) * | 2014-06-23 | 2020-09-01 | Regeneron Pharma | Montaje de ADN mediado por nucleasas |
| WO2016109255A1 (en) * | 2014-12-30 | 2016-07-07 | University Of South Florida | Methods and compositions for cloning into large vectors |
| AU2016279077A1 (en) * | 2015-06-18 | 2019-03-28 | Omar O. Abudayyeh | Novel CRISPR enzymes and systems |
| WO2016205759A1 (en) * | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation |
| US9790490B2 (en) * | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| US20190330659A1 (en) | 2016-07-15 | 2019-10-31 | Zymergen Inc. | Scarless dna assembly and genome editing using crispr/cpf1 and dna ligase |
| WO2017037304A2 (en) * | 2016-07-28 | 2017-03-09 | Dsm Ip Assets B.V. | An assembly system for a eukaryotic cell |
| JP2020507312A (ja) | 2017-02-10 | 2020-03-12 | ザイマージェン インコーポレイテッド | 複数の宿主用の複数のｄｎａコンストラクトのアセンブリ及び編集のためのモジュラーユニバーサルプラスミド設計戦略 |
| CA3107002A1 (en) * | 2018-08-15 | 2020-04-30 | Zymergen Inc. | Applications of crispri in high throughput metabolic engineering |
-
2018
- 2018-02-09 JP JP2019539756A patent/JP2020507312A/ja not_active Withdrawn
- 2018-02-09 CN CN201880012880.0A patent/CN110312797A/zh active Pending
- 2018-02-09 EP EP18751852.7A patent/EP3580337A4/en not_active Withdrawn
- 2018-02-09 WO PCT/US2018/017573 patent/WO2018148511A1/en not_active Ceased
- 2018-02-09 KR KR1020197022014A patent/KR20190116282A/ko not_active Withdrawn
- 2018-02-09 CA CA3049989A patent/CA3049989A1/en active Pending
-
2019
- 2019-08-08 US US16/535,245 patent/US11098305B2/en not_active Expired - Fee Related
-
2021
- 2021-08-03 US US17/392,366 patent/US20210371856A1/en not_active Abandoned
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