KR20190116282A - 복수의 숙주를 위한 다중 dna 구조체의 조립 및 편집을 위한 모듈식 범용 플라스미드 디자인 전략 - Google Patents

복수의 숙주를 위한 다중 dna 구조체의 조립 및 편집을 위한 모듈식 범용 플라스미드 디자인 전략 Download PDF

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KR20190116282A
KR20190116282A KR1020197022014A KR20197022014A KR20190116282A KR 20190116282 A KR20190116282 A KR 20190116282A KR 1020197022014 A KR1020197022014 A KR 1020197022014A KR 20197022014 A KR20197022014 A KR 20197022014A KR 20190116282 A KR20190116282 A KR 20190116282A
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브라이언 제이켄드
로섬 헨드릭 마리누스 반
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지머젠 인코포레이티드
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KR1020197022014A 2017-02-10 2018-02-09 복수의 숙주를 위한 다중 dna 구조체의 조립 및 편집을 위한 모듈식 범용 플라스미드 디자인 전략 Withdrawn KR20190116282A (ko)

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US201762457493P 2017-02-10 2017-02-10
US62/457,493 2017-02-10
PCT/US2018/017573 WO2018148511A1 (en) 2017-02-10 2018-02-09 A modular universal plasmid design strategy for the assembly and editing of multiple dna constructs for multiple hosts

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KR20190116282A true KR20190116282A (ko) 2019-10-14

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KR1020197022014A Withdrawn KR20190116282A (ko) 2017-02-10 2018-02-09 복수의 숙주를 위한 다중 dna 구조체의 조립 및 편집을 위한 모듈식 범용 플라스미드 디자인 전략

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US (2) US11098305B2 (https=)
EP (1) EP3580337A4 (https=)
JP (1) JP2020507312A (https=)
KR (1) KR20190116282A (https=)
CN (1) CN110312797A (https=)
CA (1) CA3049989A1 (https=)
WO (1) WO2018148511A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102178813B1 (ko) 2019-10-18 2020-11-13 주식회사 다우진유전자연구소 Dna 구조체를 적용한 간이 키트
KR20210044164A (ko) * 2019-10-14 2021-04-22 연세대학교 산학협력단 신규 프로토스페이서 인접 모티프 서열 및 이를 이용한 세포의 유전체에서 표적 핵산을 변형시키는 방법

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2020507312A (ja) 2017-02-10 2020-03-12 ザイマージェン インコーポレイテッド 複数の宿主用の複数のdnaコンストラクトのアセンブリ及び編集のためのモジュラーユニバーサルプラスミド設計戦略
CA3107002A1 (en) * 2018-08-15 2020-04-30 Zymergen Inc. Applications of crispri in high throughput metabolic engineering
RU2771374C1 (ru) 2019-04-04 2022-05-04 Редженерон Фармасьютикалс, Инк. Способы для бесшовного внесения целевых модификаций в направленные векторы
EP4063500A4 (en) * 2019-11-18 2023-12-27 Suzhou Qi Biodesign biotechnology Company Limited GENE EDITING SYSTEM DERIVED FROM BACTERIA OF THE GENUS FLAVOBACTERIUM
EP4065701A4 (en) * 2019-11-27 2023-11-29 Danmarks Tekniske Universitet CONSTRUCTS, COMPOSITIONS AND METHODS THEREOF WITH IMPROVED GENOMEDITING EFFICIENCY AND SPECIFICITY
JP7742355B2 (ja) 2020-03-03 2025-09-19 コーデックス ディーエヌエー インコーポレイテッド 核酸をアセンブルするための方法
CN113373130B (zh) * 2021-05-31 2023-12-22 复旦大学 Cas12蛋白、含有Cas12蛋白的基因编辑系统及应用
GB202117455D0 (en) * 2021-12-02 2022-01-19 Academisch Ziekenhuis Leiden Method of editing nucleic acid
KR20240169697A (ko) * 2022-04-08 2024-12-03 글락소스미스클라인 인털렉츄얼 프로퍼티 디벨로프먼트 리미티드 올리고뉴클레오티드를 포함한 폴리뉴클레오티드의 신규 생산 방법
CN116284436B (zh) * 2022-11-25 2026-04-07 广州美格生物科技有限公司 嵌合体TsCas12a蛋白及其制备方法和应用
CN119432813A (zh) * 2023-12-29 2025-02-14 武汉艾迪晶生物科技有限公司 Cas蛋白、组合蛋白、核酸分子、核糖核蛋白复合物、重组载体、转基因细胞及其应用
WO2025165780A1 (en) * 2024-02-01 2025-08-07 The Board Of Trustees Of The Leland Stanford Junior University Combinatorial gene editing and screening

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2204913T3 (es) 1993-04-12 2004-05-01 Northwestern University Metodo para formacion de oligonucleotidos.
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
EP1127135B1 (en) 1998-10-30 2007-05-23 Cornell Research Foundation, Inc. High fidelity thermostable ligase and uses thereof
KR20100133319A (ko) * 2009-06-11 2010-12-21 주식회사 툴젠 표적 특이적인 게놈의 재배열을 위한 표적 특이적 뉴클레아제 및 이의 용도
GB2481425A (en) * 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
AU2013266968B2 (en) 2012-05-25 2017-06-29 Emmanuelle CHARPENTIER Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription
CN113355357B (zh) 2012-12-12 2024-12-03 布罗德研究所有限公司 对用于序列操纵的改进的系统、方法和酶组合物进行的工程化和优化
IL239344B2 (en) 2012-12-12 2024-06-01 Broad Inst Inc Systems engineering, methods and optimal guiding components for sequence manipulation
WO2014093655A2 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
EP2840140B2 (en) 2012-12-12 2023-02-22 The Broad Institute, Inc. Crispr-Cas based method for mutation of prokaryotic cells
US20140310830A1 (en) 2012-12-12 2014-10-16 Feng Zhang CRISPR-Cas Nickase Systems, Methods And Compositions For Sequence Manipulation in Eukaryotes
CN112301024A (zh) * 2013-03-15 2021-02-02 通用医疗公司 使用RNA引导的FokI核酸酶(RFN)提高RNA引导的基因组编辑的特异性
US9873907B2 (en) 2013-05-29 2018-01-23 Agilent Technologies, Inc. Method for fragmenting genomic DNA using CAS9
WO2014197748A2 (en) 2013-06-05 2014-12-11 Duke University Rna-guided gene editing and gene regulation
RU2716420C2 (ru) * 2013-06-17 2020-03-11 Те Брод Инститьют Инк. Доставка и применение систем crispr-cas, векторов и композиций для целенаправленного воздействия и терапии в печени
WO2014204724A1 (en) * 2013-06-17 2014-12-24 The Broad Institute Inc. Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation
US10287590B2 (en) 2014-02-12 2019-05-14 Dna2.0, Inc. Methods for generating libraries with co-varying regions of polynuleotides for genome modification
ES2781323T3 (es) * 2014-06-23 2020-09-01 Regeneron Pharma Montaje de ADN mediado por nucleasas
WO2016109255A1 (en) * 2014-12-30 2016-07-07 University Of South Florida Methods and compositions for cloning into large vectors
AU2016279077A1 (en) * 2015-06-18 2019-03-28 Omar O. Abudayyeh Novel CRISPR enzymes and systems
WO2016205759A1 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation
US9790490B2 (en) * 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
US20190330659A1 (en) 2016-07-15 2019-10-31 Zymergen Inc. Scarless dna assembly and genome editing using crispr/cpf1 and dna ligase
WO2017037304A2 (en) * 2016-07-28 2017-03-09 Dsm Ip Assets B.V. An assembly system for a eukaryotic cell
JP2020507312A (ja) 2017-02-10 2020-03-12 ザイマージェン インコーポレイテッド 複数の宿主用の複数のdnaコンストラクトのアセンブリ及び編集のためのモジュラーユニバーサルプラスミド設計戦略
CA3107002A1 (en) * 2018-08-15 2020-04-30 Zymergen Inc. Applications of crispri in high throughput metabolic engineering

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210044164A (ko) * 2019-10-14 2021-04-22 연세대학교 산학협력단 신규 프로토스페이서 인접 모티프 서열 및 이를 이용한 세포의 유전체에서 표적 핵산을 변형시키는 방법
KR102178813B1 (ko) 2019-10-18 2020-11-13 주식회사 다우진유전자연구소 Dna 구조체를 적용한 간이 키트

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US20200123540A1 (en) 2020-04-23
CA3049989A1 (en) 2018-08-16
WO2018148511A1 (en) 2018-08-16
US20210371856A1 (en) 2021-12-02
CN110312797A (zh) 2019-10-08
EP3580337A1 (en) 2019-12-18
JP2020507312A (ja) 2020-03-12
EP3580337A4 (en) 2020-12-02
US11098305B2 (en) 2021-08-24

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