JP2020501577A5 - - Google Patents
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- JP2020501577A5 JP2020501577A5 JP2019533340A JP2019533340A JP2020501577A5 JP 2020501577 A5 JP2020501577 A5 JP 2020501577A5 JP 2019533340 A JP2019533340 A JP 2019533340A JP 2019533340 A JP2019533340 A JP 2019533340A JP 2020501577 A5 JP2020501577 A5 JP 2020501577A5
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- solution
- affinity ligand
- light chain
- stationary phase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 description 8
- 239000000126 substance Substances 0.000 description 7
- 239000003446 ligand Substances 0.000 description 6
- 230000005526 G1 to G0 transition Effects 0.000 description 5
- 230000004048 modification Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Chemical group CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 3
- PNNNRSAQSRJVSB-SLPGGIOYSA-N Fucose Chemical group C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C=O PNNNRSAQSRJVSB-SLPGGIOYSA-N 0.000 description 3
- SHZGCJCMOBCMKK-DHVFOXMCSA-N L-fucopyranose Chemical group C[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@@H]1O SHZGCJCMOBCMKK-DHVFOXMCSA-N 0.000 description 3
- 230000004988 N-glycosylation Effects 0.000 description 3
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical group OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 3
- 230000013595 glycosylation Effects 0.000 description 3
- 238000006206 glycosylation reaction Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 1
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 238000011091 antibody purification Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 238000011026 diafiltration Methods 0.000 description 1
- 238000011038 discontinuous diafiltration by volume reduction Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000009144 enzymatic modification Effects 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP16205587.5 | 2016-12-21 | ||
| EP16205587 | 2016-12-21 | ||
| EP17157002.1 | 2017-02-20 | ||
| EP17157002 | 2017-02-20 | ||
| PCT/EP2017/083429 WO2018114877A1 (en) | 2016-12-21 | 2017-12-19 | In vitro glycoengineering of antibodies |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020501577A JP2020501577A (ja) | 2020-01-23 |
| JP2020501577A5 true JP2020501577A5 (enExample) | 2021-01-07 |
| JP6850351B2 JP6850351B2 (ja) | 2021-03-31 |
Family
ID=60997429
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019533340A Active JP6850351B2 (ja) | 2016-12-21 | 2017-12-19 | 抗体のインビトロ糖鎖工学 |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US20200165320A1 (enExample) |
| EP (1) | EP3559248B2 (enExample) |
| JP (1) | JP6850351B2 (enExample) |
| KR (1) | KR102317884B1 (enExample) |
| CN (2) | CN119685431A (enExample) |
| AU (1) | AU2017384276B9 (enExample) |
| CA (1) | CA3044920C (enExample) |
| IL (1) | IL267351A (enExample) |
| MX (1) | MX2019006266A (enExample) |
| TW (1) | TWI780097B (enExample) |
| WO (1) | WO2018114877A1 (enExample) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6850351B2 (ja) | 2016-12-21 | 2021-03-31 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 抗体のインビトロ糖鎖工学 |
| EP3559250A1 (en) | 2016-12-21 | 2019-10-30 | H. Hoffnabb-La Roche Ag | Re-use of enzymes in in vitro glycoengineering of antibodies |
| AU2017381657B2 (en) * | 2016-12-21 | 2020-07-23 | F. Hoffmann-La Roche Ag | Method for in vitro glycoengineering of antibodies |
| CA3106146A1 (en) | 2018-07-13 | 2020-01-16 | Genmab A/S | Variants of cd38 antibody and uses thereof |
| US20240254252A1 (en) | 2018-07-13 | 2024-08-01 | Genmab A/S | Trogocytosis-mediated therapy using cd38 antibodies |
| PE20230113A1 (es) | 2020-01-16 | 2023-01-27 | Genmab As | Formulaciones de anticuerpos anti-cd38 y usos de las mismas |
| KR20240051280A (ko) | 2021-09-06 | 2024-04-19 | 젠맵 에이/에스 | Cd27과 결합할 수 있는 항체, 그의 변이체 및 그의 용도 |
| AR132668A1 (es) | 2023-05-12 | 2025-07-16 | Genmab As | Anticuerpos capaces de unirse a ox40, variantes de los mismos y usos de los mismos |
| WO2025049881A1 (en) * | 2023-09-01 | 2025-03-06 | Catalent Pharma Solutions, Llc | Dnase co-expression in host cells |
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| BR112014019825B1 (pt) * | 2012-02-10 | 2021-08-24 | University Of Maryland, Baltimore | Glicoengenharia quimioenzimática de anticorpos e fragmentos fc dos mesmos |
| WO2015123754A1 (en) | 2014-02-18 | 2015-08-27 | The University Of Manitoba | Methods to produce single glycoform antibodies |
| CN114181312A (zh) | 2014-09-10 | 2022-03-15 | 豪夫迈·罗氏有限公司 | 半乳糖改造的免疫球蛋白1抗体 |
| JP6850351B2 (ja) | 2016-12-21 | 2021-03-31 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 抗体のインビトロ糖鎖工学 |
-
2017
- 2017-12-19 JP JP2019533340A patent/JP6850351B2/ja active Active
- 2017-12-19 EP EP17829955.8A patent/EP3559248B2/en active Active
- 2017-12-19 MX MX2019006266A patent/MX2019006266A/es unknown
- 2017-12-19 KR KR1020197017701A patent/KR102317884B1/ko active Active
- 2017-12-19 WO PCT/EP2017/083429 patent/WO2018114877A1/en not_active Ceased
- 2017-12-19 AU AU2017384276A patent/AU2017384276B9/en active Active
- 2017-12-19 CN CN202411870260.4A patent/CN119685431A/zh active Pending
- 2017-12-19 CA CA3044920A patent/CA3044920C/en active Active
- 2017-12-19 CN CN201780079057.7A patent/CN110088291A/zh active Pending
- 2017-12-20 TW TW106144931A patent/TWI780097B/zh active
-
2019
- 2019-06-13 IL IL267351A patent/IL267351A/en unknown
- 2019-06-20 US US16/447,093 patent/US20200165320A1/en not_active Abandoned
-
2023
- 2023-05-16 US US18/318,487 patent/US20240101643A1/en active Pending
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