JP2020182453A5 - - Google Patents

Download PDF

Info

Publication number
JP2020182453A5
JP2020182453A5 JP2020076547A JP2020076547A JP2020182453A5 JP 2020182453 A5 JP2020182453 A5 JP 2020182453A5 JP 2020076547 A JP2020076547 A JP 2020076547A JP 2020076547 A JP2020076547 A JP 2020076547A JP 2020182453 A5 JP2020182453 A5 JP 2020182453A5
Authority
JP
Japan
Prior art keywords
primer
target
nucleic acid
acid sequence
tail
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2020076547A
Other languages
English (en)
Japanese (ja)
Other versions
JP2020182453A (ja
Filing date
Publication date
Application filed filed Critical
Publication of JP2020182453A publication Critical patent/JP2020182453A/ja
Publication of JP2020182453A5 publication Critical patent/JP2020182453A5/ja
Pending legal-status Critical Current

Links

JP2020076547A 2014-01-27 2020-04-23 配列決定のために核酸を調製する方法 Pending JP2020182453A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461931959P 2014-01-27 2014-01-27
US61/931,959 2014-01-27

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
JP2016549249A Division JP7016215B2 (ja) 2014-01-27 2015-01-26 配列決定のために核酸を調製する方法

Publications (2)

Publication Number Publication Date
JP2020182453A JP2020182453A (ja) 2020-11-12
JP2020182453A5 true JP2020182453A5 (cg-RX-API-DMAC7.html) 2021-02-25

Family

ID=53678460

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2016549249A Active JP7016215B2 (ja) 2014-01-27 2015-01-26 配列決定のために核酸を調製する方法
JP2020076547A Pending JP2020182453A (ja) 2014-01-27 2020-04-23 配列決定のために核酸を調製する方法

Family Applications Before (1)

Application Number Title Priority Date Filing Date
JP2016549249A Active JP7016215B2 (ja) 2014-01-27 2015-01-26 配列決定のために核酸を調製する方法

Country Status (6)

Country Link
US (4) US10450597B2 (cg-RX-API-DMAC7.html)
EP (2) EP4219744A3 (cg-RX-API-DMAC7.html)
JP (2) JP7016215B2 (cg-RX-API-DMAC7.html)
CN (1) CN106661612A (cg-RX-API-DMAC7.html)
AU (2) AU2015209079B2 (cg-RX-API-DMAC7.html)
WO (1) WO2015112974A1 (cg-RX-API-DMAC7.html)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US8825412B2 (en) 2010-05-18 2014-09-02 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
RU2671980C2 (ru) 2011-02-09 2018-11-08 Натера, Инк. Способы неинвазивного пренатального установления плоидности
US10941396B2 (en) 2012-02-27 2021-03-09 Becton, Dickinson And Company Compositions and kits for molecular counting
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
EP3039158B1 (en) 2013-08-28 2018-11-14 Cellular Research, Inc. Massively parallel single cell analysis
ES2819277T3 (es) 2014-02-11 2021-04-15 Hoffmann La Roche Secuenciación dirigida y filtrado de UID
US12492429B2 (en) 2014-04-21 2025-12-09 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
JP6659575B2 (ja) 2014-04-21 2020-03-04 ナテラ, インコーポレイテッド 変異の検出および染色体分節の倍数性
US20180173845A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
US20160257993A1 (en) * 2015-02-27 2016-09-08 Cellular Research, Inc. Methods and compositions for labeling targets
US9727810B2 (en) 2015-02-27 2017-08-08 Cellular Research, Inc. Spatially addressable molecular barcoding
JP7508191B2 (ja) 2015-03-30 2024-07-01 ベクトン・ディキンソン・アンド・カンパニー コンビナトリアルバーコーディングのための方法および組成物
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
EP4428863A3 (en) 2015-05-11 2024-12-11 Natera, Inc. Methods and compositions for determining ploidy
WO2017021449A1 (en) 2015-08-06 2017-02-09 F. Hoffmann-La Roche Ag Target enrichment by single probe primer extension
KR102395450B1 (ko) 2015-09-11 2022-05-09 셀룰러 리서치, 인크. 핵산 라이브러리 정규화를 위한 방법 및 조성물
AU2016362875B2 (en) * 2015-12-01 2022-12-08 LGC Clinical Diagnostics, Inc. Multiplex cellular reference materials
WO2017181202A2 (en) 2016-04-15 2017-10-19 Natera, Inc. Methods for lung cancer detection
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
WO2017210469A2 (en) 2016-06-01 2017-12-07 F. Hoffman-La Roche Ag Immuno-pete
EP3485038B1 (en) * 2016-07-12 2020-12-23 H. Hoffnabb-La Roche Ag Primer extension target enrichment
US20220282305A1 (en) * 2016-09-15 2022-09-08 Archerdx, Llc Methods of nucleic acid sample preparation
CN110023504B (zh) * 2016-09-15 2023-05-09 阿谢尔德克斯有限责任公司 用于分析无细胞dna的核酸样品制备方法
ES2903357T3 (es) 2016-09-15 2022-04-01 Archerdx Llc Métodos de preparación de muestras de ácido nucleico
EP3516400B1 (en) 2016-09-26 2023-08-16 Becton, Dickinson and Company Measurement of protein expression using reagents with barcoded oligonucleotide sequences
US11485996B2 (en) 2016-10-04 2022-11-01 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
US11667951B2 (en) 2016-10-24 2023-06-06 Geneinfosec, Inc. Concealing information present within nucleic acids
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
CA3042391A1 (en) * 2016-11-02 2018-05-11 ArcherDX, Inc. Methods of nucleic acid sample preparation for immune repertoire sequencing
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
CN106755451A (zh) * 2017-01-05 2017-05-31 苏州艾达康医疗科技有限公司 核酸制备及分析
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
US12297491B2 (en) 2017-03-08 2025-05-13 Roche Sequencing Solutions, Inc. Primer extension target enrichment and improvements thereto including simultaneous enrichment of DNA and RNA
JP6970205B2 (ja) 2017-03-08 2021-11-24 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft Dnaおよびrnaの同時濃縮を含むプライマー伸長標的濃縮およびそれに対する向上
JP7536450B2 (ja) 2017-06-05 2024-08-20 ベクトン・ディキンソン・アンド・カンパニー 単一細胞用のサンプルインデックス付加
US11505826B2 (en) * 2017-07-12 2022-11-22 Agilent Technologies, Inc. Sequencing method for genomic rearrangement detection
JP6998404B2 (ja) * 2017-08-01 2022-02-04 深▲セン▼恒特基因有限公司 標的ヌクレオチド配列の富化及び決定方法
WO2019090482A1 (zh) * 2017-11-07 2019-05-16 北京优乐复生科技有限责任公司 一种第二代高通量测序文库构建方法
CA3085933A1 (en) 2017-12-14 2019-06-20 Tai Diagnostics, Inc. Assessing graft suitability for transplantation
JP2021506314A (ja) * 2017-12-21 2021-02-22 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 単一方向デュアルプローブプライマー伸長による標的濃縮
CN110241209B (zh) * 2018-03-09 2022-11-29 浙江品级基因科技有限公司 一种引物、试剂盒及用途
AU2019251504B2 (en) 2018-04-14 2025-09-25 Natera, Inc. Methods for cancer detection and monitoring by means of personalized detection of circulating tumor DNA
CN112272710A (zh) 2018-05-03 2021-01-26 贝克顿迪金森公司 高通量多组学样品分析
CN118910215A (zh) 2018-05-03 2024-11-08 贝克顿迪金森公司 在相对的转录物末端进行分子条形码化
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
US11639517B2 (en) 2018-10-01 2023-05-02 Becton, Dickinson And Company Determining 5′ transcript sequences
US11932849B2 (en) 2018-11-08 2024-03-19 Becton, Dickinson And Company Whole transcriptome analysis of single cells using random priming
JP2022509868A (ja) 2018-11-30 2022-01-24 ジェネインフォセック、インコーポレイテッド ランダムオリゴヌクレオチドの生成方法およびその配列決定方法
CN113195717A (zh) 2018-12-13 2021-07-30 贝克顿迪金森公司 单细胞全转录组分析中的选择性延伸
EP4242322B1 (en) 2019-01-23 2024-08-21 Becton, Dickinson and Company Oligonucleotides associated with antibodies
US12071617B2 (en) 2019-02-14 2024-08-27 Becton, Dickinson And Company Hybrid targeted and whole transcriptome amplification
EP3980559A1 (en) 2019-06-06 2022-04-13 Natera, Inc. Methods for detecting immune cell dna and monitoring immune system
US11939622B2 (en) 2019-07-22 2024-03-26 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
JP7522189B2 (ja) 2019-11-08 2024-07-24 ベクトン・ディキンソン・アンド・カンパニー 免疫レパートリーシーケンシングのための完全長v(d)j情報を得るためのランダムプライミングの使用
WO2021146207A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and rna
ES2993319T3 (en) 2020-01-29 2024-12-27 Becton Dickinson Co Barcoded wells for spatial mapping of single cells through sequencing
CN115151810A (zh) 2020-02-25 2022-10-04 贝克顿迪金森公司 实现使用单细胞样品作为单色补偿对照的双特异性探针
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司(Us) 用于免疫组库谱分析的引物
CN115803445A (zh) 2020-06-02 2023-03-14 贝克顿迪金森公司 用于5撇基因表达测定的寡核苷酸和珠
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
EP4240842B1 (en) * 2020-11-03 2024-12-18 ACT Genomics (IP) Limited Targeted sequencing method and kit thereof for detecting gene alteration
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
US20250243531A1 (en) * 2021-12-14 2025-07-31 Arizona Board Of Regents On Behalf Of Arizona State University Methods for combining in situ single cell dna and rna sequencing

Family Cites Families (108)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4868104A (en) 1985-09-06 1989-09-19 Syntex (U.S.A.) Inc. Homogeneous assay for specific polynucleotides
US6027913A (en) 1988-01-28 2000-02-22 Sommer; Steven S. Nucleic acid amplification with direct sequencing
GB8818020D0 (en) 1988-07-28 1988-09-01 Ici Plc Method for amplification of nucleotide sequences
US6087101A (en) 1990-05-18 2000-07-11 Gruelich; Karl Otto Optical characterization of nucleic acids and oligonucleotides
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5565340A (en) 1995-01-27 1996-10-15 Clontech Laboratories, Inc. Method for suppressing DNA fragment amplification during PCR
CA2239683A1 (en) 1995-12-22 1997-07-03 Dade Behring Marburg Gmbh Homogeneous amplification and detection of nucleic acids
US6013439A (en) 1995-12-22 2000-01-11 Dade Behring Marburg Gmbh Detection of differences in nucleic acids
US5827658A (en) 1996-08-09 1998-10-27 The United States Of America As Reprsented By The Department Of Health And Human Services Isolation of amplified genes via cDNA subtractive hybridization
US6482590B1 (en) 1996-12-20 2002-11-19 Aventis Behring Gmbh Method for polynucleotide amplification
US6365346B1 (en) 1998-02-18 2002-04-02 Dade Behring Inc. Quantitative determination of nucleic acid amplification products
US6235502B1 (en) 1998-09-18 2001-05-22 Molecular Staging Inc. Methods for selectively isolating DNA using rolling circle amplification
US6200757B1 (en) 1999-01-19 2001-03-13 Dade Behring Inc. Method for controlling the extension of an oligonucleotide
WO2000043543A1 (en) 1999-01-19 2000-07-27 Dade Behring Inc. Detection of differences between polynucleotides
WO2000070095A2 (en) 1999-05-17 2000-11-23 Dade Behring Inc. Homogeneous isothermal amplification and detection of nucleic acids using a template switch oligonucleotide
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6232104B1 (en) 1999-08-17 2001-05-15 Dade Behring Inc. Detection of differences in nucleic acids by inhibition of spontaneous DNA branch migration
BR0014182A (pt) 1999-09-13 2002-05-21 Nugen Technologies Inc Processos e composições para amplificação isotérmica linear de sequências polinucleotìdicas
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
WO2001023610A2 (en) * 1999-09-29 2001-04-05 Solexa Ltd. Polynucleotide sequencing
US6309836B1 (en) 1999-10-05 2001-10-30 Marek Kwiatkowski Compounds for protecting hydroxyls and methods for their use
WO2001083696A2 (en) 2000-04-28 2001-11-08 Digital Gene Tech Inc Methods for rapid isolation and sequence determination of gene-specific sequences
DE60141087D1 (de) 2000-06-26 2010-03-04 Nugen Technologies Inc Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren
EP1176212A1 (en) 2000-07-24 2002-01-30 Centre National de Genotype Method for haplotyping by mass spectrometry
US6815164B2 (en) 2000-10-06 2004-11-09 Nugen Technologies, Inc. Methods and probes for detection and/or quantification of nucleic acid sequences
JP2002153271A (ja) * 2000-11-17 2002-05-28 Jeol Ltd Dnaあるいはrnaの塩基配列決定方法およびdnaシーケンサー
CA2430329A1 (en) 2000-12-13 2002-06-20 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
CN1289690C (zh) 2001-03-09 2006-12-13 纽亘技术公司 Rna序列扩增的方法和组合物
AU2005203617A1 (en) 2001-03-09 2005-09-01 Nugen Technologies, Inc Methods and compositions for amplification of RNA sequences
US6632611B2 (en) 2001-07-20 2003-10-14 Affymetrix, Inc. Method of target enrichment and amplification
US20030104432A1 (en) 2001-07-27 2003-06-05 The Regents Of The University Of California Methods of amplifying sense strand RNA
DE60323067D1 (de) 2002-03-11 2008-10-02 Nugen Technologies Inc Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination
JP2005521409A (ja) 2002-03-29 2005-07-21 ニューゲン テクノロジーズ, インコーポレイテッド 単一プライマー等温核酸増幅−増強型被分析物検出および定量
US20040005614A1 (en) 2002-05-17 2004-01-08 Nurith Kurn Methods for fragmentation, labeling and immobilization of nucleic acids
WO2003100019A2 (en) 2002-05-24 2003-12-04 Invitrogen Corporation Nested pcr employing degradable primers
US7459273B2 (en) 2002-10-04 2008-12-02 Affymetrix, Inc. Methods for genotyping selected polymorphism
DE602004026033D1 (de) 2003-01-29 2010-04-29 454 Corp Zweiendige sequenzierung
ATE328112T1 (de) 2003-03-07 2006-06-15 Ist Naz Stud Cura Dei Tumori Anaplastisches lymphoma kinase testverfahren, reagenzien und kompositionen davon
JP2006523465A (ja) 2003-04-14 2006-10-19 ニューゲン テクノロジーズ, インコーポレイテッド ランダムにプライミングされる複合プライマーを用いる大規模増幅
US7226720B2 (en) 2003-09-08 2007-06-05 General Electric Company Limited play data storage media and method for limiting access to data thereon
SG149007A1 (en) 2003-09-10 2009-01-29 Althea Technologies Inc Expression profiling using microarrays
WO2005045073A1 (en) * 2003-11-10 2005-05-19 Seegene, Inc. Method for amplifying unknown dna sequence adjacent to known sequence
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
JP5138938B2 (ja) 2003-12-19 2013-02-06 プレキシコン インコーポレーテッド Ret調節剤の開発のための化合物および方法
EP1711591A4 (en) 2003-12-29 2010-04-28 Nugen Technologies Inc METHODS FOR ANALYZING THE STATE OF METHYLATION OF NUCLEIC ACIDS AND METHODS FOR FRAGMENTING, MARKING AND IMMOBILIZING NUCLEIC ACIDS
JP3972106B2 (ja) 2004-03-03 2007-09-05 大学共同利用機関法人情報・システム研究機構 ゲノムライブラリー作製方法、および同方法により作製されたゲノムライブラリー
GB0421525D0 (en) 2004-09-28 2004-10-27 Novartis Ag Inhibitors of protein kineses
US20070070349A1 (en) 2005-09-23 2007-03-29 Helicos Biosciences Corporation Optical train and method for TIRF single molecule detection and analysis
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
EP1929046B1 (en) 2005-09-07 2012-07-11 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
US20070141604A1 (en) 2005-11-15 2007-06-21 Gormley Niall A Method of target enrichment
US7282337B1 (en) 2006-04-14 2007-10-16 Helicos Biosciences Corporation Methods for increasing accuracy of nucleic acid sequencing
EP2010670B1 (en) 2006-05-04 2012-11-28 Seegene, Inc. Method for amplifying unknown dna sequence adjacent to known sequence
CA2652052A1 (en) 2006-05-16 2007-11-29 Nugen Technologies, Inc. Nucleic acid separation and purification method based on reversible charge interactions
EP2038429B1 (en) 2006-06-30 2013-08-21 Nugen Technologies, Inc. Methods for fragmentation and labeling of nucleic acids
EP3285067B1 (en) 2006-12-14 2022-06-22 Life Technologies Corporation Apparatus for measuring analytes using fet arrays
EP2121983A2 (en) 2007-02-02 2009-11-25 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US8716190B2 (en) 2007-09-14 2014-05-06 Affymetrix, Inc. Amplification and analysis of selected targets on solid supports
JP2011500063A (ja) 2007-10-22 2011-01-06 モノクアント・ピーティーワイ・リミテッド Dna増幅方法
US8034568B2 (en) * 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
EP2250283A2 (en) 2008-02-12 2010-11-17 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
US7846666B2 (en) 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
US8029993B2 (en) 2008-04-30 2011-10-04 Population Genetics Technologies Ltd. Asymmetric adapter library construction
US20110172105A1 (en) 2008-06-04 2011-07-14 Salk Institute For Biological Studies GREPSEQ: An Almost Inexhaustible, Cost-Effective, High-Throughput Protocol for the Generation of Selector Sequences
EP2313091A4 (en) 2008-07-14 2012-04-04 Univ Kingston PHARMACEUTICAL COMPOSITIONS WITH RET-HEMMERN AND METHOD FOR THE TREATMENT OF CANCER
WO2010077288A2 (en) 2008-12-09 2010-07-08 The Salk Institute For Biological Studies Methods for identifying differences in alternative splicing between two rna samples
WO2010083046A2 (en) 2009-01-15 2010-07-22 The Salk Institute For Biological Studies Methods for using next generation sequencing to identify 5-methyl cytosines in the genome
US20100286143A1 (en) * 2009-04-24 2010-11-11 Dora Dias-Santagata Methods and materials for genetic analysis of tumors
WO2011019964A1 (en) 2009-08-12 2011-02-17 Nugen Technologies, Inc. Methods, compositions, and kits for generating nucleic acid products substantially free of template nucleic acid
US20110189679A1 (en) 2009-09-11 2011-08-04 Nugen Technologies, Inc. Compositions and methods for whole transcriptome analysis
US20110105364A1 (en) 2009-11-02 2011-05-05 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence selection and amplification
US8574864B2 (en) 2009-11-05 2013-11-05 Epicentre Technologies Corporation Methods and kits for 3'-end-tagging of RNA
EP2545189B1 (en) * 2010-03-08 2018-01-10 Dana-Farber Cancer Institute, Inc. Full cold-pcr enrichment with reference blocking sequence
US20110269647A1 (en) 2010-04-28 2011-11-03 Medical Research Council Method
US8825412B2 (en) 2010-05-18 2014-09-02 Natera, Inc. Methods for non-invasive prenatal ploidy calling
EP2580378A4 (en) 2010-06-08 2014-01-01 Nugen Technologies Inc MULTIPLEX SEQUENCING METHODS AND COMPOSITION
WO2012003374A2 (en) 2010-07-02 2012-01-05 The Board Of Trustees Of The Leland Stanford Junior University Targeted sequencing library preparation by genomic dna circularization
US8481292B2 (en) 2010-09-21 2013-07-09 Population Genetics Technologies Litd. Increasing confidence of allele calls with molecular counting
KR20130113447A (ko) 2010-09-24 2013-10-15 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 고정된 프라이머들을 이용하여 표적 dna의 직접적인 캡쳐, 증폭 및 서열화
US20120100549A1 (en) * 2010-10-01 2012-04-26 Ibis Biosciences, Inc. Targeted genome amplification methods
ES2688458T5 (es) 2010-10-22 2022-04-13 Cold Spring Harbor Laboratory Recuento varietal de ácidos nucleicos para obtener información del número de copias genómicas
WO2012064739A2 (en) 2010-11-08 2012-05-18 The Trustees Of Columbia University In The City Of New York Microbial enrichment primers
EP2656263B1 (en) 2010-12-22 2019-11-06 Natera, Inc. Methods for non-invasive prenatal paternity testing
WO2012103154A1 (en) 2011-01-24 2012-08-02 Nugen Technologies, Inc. Stem-loop composite rna-dna adaptor-primers: compositions and methods for library generation, amplification and other downstream manipulations
RU2671980C2 (ru) 2011-02-09 2018-11-08 Натера, Инк. Способы неинвазивного пренатального установления плоидности
EP3736281A1 (en) 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
WO2012131616A1 (en) * 2011-03-30 2012-10-04 Chromous Biotech Pvt. Ltd. Primer set, kit and methods thereof
PL3246416T3 (pl) 2011-04-15 2024-09-30 The Johns Hopkins University Bezpieczny system sekwencjonowania
US9074204B2 (en) * 2011-05-20 2015-07-07 Fluidigm Corporation Nucleic acid encoding reactions
US9617598B2 (en) 2011-05-27 2017-04-11 President And Fellows Of Harvard College Methods of amplifying whole genome of a single cell
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
WO2013059746A1 (en) 2011-10-19 2013-04-25 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
WO2013074833A1 (en) 2011-11-16 2013-05-23 The Board Of Trustees Of The Leland Stanford Junior University Capture probe and assay for analysis of fragmented nucleic acids
EP3578697B1 (en) 2012-01-26 2024-03-06 Tecan Genomics, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
US20150045258A1 (en) 2012-02-14 2015-02-12 Gnubio, Inc. Cascaded addition of target specific universal adapters to nucleic acids
DK3363901T3 (da) 2012-02-17 2021-02-22 Hutchinson Fred Cancer Res Sammensætninger og fremgangsmåder til præcis identificering af mutationer
CA2873176C (en) 2012-05-10 2024-02-27 The General Hospital Corporation Methods for determining a nucleotide sequence
CN104619894B (zh) 2012-06-18 2017-06-06 纽亘技术公司 用于非期望核酸序列的阴性选择的组合物和方法
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
WO2014047678A1 (en) 2012-09-25 2014-04-03 Agriculture Victoria Services Pty Ltd Method of producing a normalised nucleic acid library using solid state capture material
US20140274729A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Methods, compositions and kits for generation of stranded rna or dna libraries
US20140274738A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
EP3068883B1 (en) 2013-11-13 2020-04-29 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
US9689027B2 (en) 2013-11-15 2017-06-27 The Board Of Trustees Of The Leland Stanford Junior University High efficiency multiplexed nucleic acid capture in a structured microenvironment
ES2903357T3 (es) 2016-09-15 2022-04-01 Archerdx Llc Métodos de preparación de muestras de ácido nucleico
CN110023504B (zh) 2016-09-15 2023-05-09 阿谢尔德克斯有限责任公司 用于分析无细胞dna的核酸样品制备方法
CA3042391A1 (en) 2016-11-02 2018-05-11 ArcherDX, Inc. Methods of nucleic acid sample preparation for immune repertoire sequencing

Similar Documents

Publication Publication Date Title
JP2020182453A5 (cg-RX-API-DMAC7.html)
KR100977186B1 (ko) 이중 특이성 올리고뉴클레오타이드를 사용한 방법 및 이중특이성 올리고뉴클레오타이드
KR101534228B1 (ko) 이중 특이성 올리고뉴클레오타이드를 사용한 방법 및 이중 특이성 올리고뉴클레오타이드
US9057110B2 (en) Method for direct amplification from crude nucleic acid samples
JP2024114703A5 (cg-RX-API-DMAC7.html)
JP2011500092A5 (cg-RX-API-DMAC7.html)
JP2020516281A5 (cg-RX-API-DMAC7.html)
CA2468754A1 (en) Annealing control primer and its uses
CA2476564A1 (en) Polynomial amplification of nucleic acids
JP2021094038A (ja) 核酸を調製するための等温方法および関連組成物
US20200232011A1 (en) Methods of nucleic acid detection and primer design
JP6847364B2 (ja) オリゴカチオン複合プライマー配列を使用する等温増幅
JP2021503903A (ja) 非対称pcr法
JP2009513142A5 (cg-RX-API-DMAC7.html)
WO2017183648A1 (ja) 多項目増幅手法
JPWO2020126602A5 (cg-RX-API-DMAC7.html)
US7501254B2 (en) Methods and compositions for amplification and capture of nucleic acid sequences
WO2012032510A1 (en) Primers for amplifying dna and methods of selecting same
CN109415762B (zh) 通过多重扩增双重信号扩增的目标核酸序列的检测方法
JP6983906B2 (ja) ライブラリーの定量および定性
JPWO2020132103A5 (cg-RX-API-DMAC7.html)
CN111334562A (zh) 一种含有修饰引物的核酸扩增方法和试剂盒
JP2024002889A (ja) Dnaライブラリーの作製キット及び作製方法、遺伝子多型の検出方法
KR20240086799A (ko) 염소의 초위성체 마커를 이용한 개체식별 및 성 판별 방법
JP2009219445A (ja) Vkorc1遺伝子上の1塩基多型を検出する方法およびプライマーセット