CN110241209B - 一种引物、试剂盒及用途 - Google Patents
一种引物、试剂盒及用途 Download PDFInfo
- Publication number
- CN110241209B CN110241209B CN201810432612.6A CN201810432612A CN110241209B CN 110241209 B CN110241209 B CN 110241209B CN 201810432612 A CN201810432612 A CN 201810432612A CN 110241209 B CN110241209 B CN 110241209B
- Authority
- CN
- China
- Prior art keywords
- primer
- sequence
- dna
- circulating tumor
- molecular barcode
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Hospice & Palliative Care (AREA)
- Biophysics (AREA)
- Oncology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (3)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2018101934488 | 2018-03-09 | ||
CN201810193448 | 2018-03-09 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN110241209A CN110241209A (zh) | 2019-09-17 |
CN110241209B true CN110241209B (zh) | 2022-11-29 |
Family
ID=67882887
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201810432612.6A Active CN110241209B (zh) | 2018-03-09 | 2018-05-08 | 一种引物、试剂盒及用途 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN110241209B (zh) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2017024784A1 (zh) * | 2015-08-10 | 2017-02-16 | 北京吉因加科技有限公司 | 一种血浆中游离的目标dna低频突变富集测序方法 |
CN106661612A (zh) * | 2014-01-27 | 2017-05-10 | 通用医疗公司 | 制备用于测序的核酸的方法 |
CN107002080A (zh) * | 2014-12-18 | 2017-08-01 | 深圳华大基因研究院 | 一种基于多重pcr的目标区域富集方法和试剂 |
CN107475403A (zh) * | 2017-09-14 | 2017-12-15 | 深圳因合生物科技有限公司 | 从外周血游离dna中检测循环肿瘤dna的方法、试剂盒及其测序结果的分析方法 |
CN107709557A (zh) * | 2015-04-15 | 2018-02-16 | 通用医疗公司 | 基于lna的突变型富集下一代测序测定 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2971152B1 (en) * | 2013-03-15 | 2018-08-01 | The Board Of Trustees Of The Leland Stanford Junior University | Identification and use of circulating nucleic acid tumor markers |
-
2018
- 2018-05-08 CN CN201810432612.6A patent/CN110241209B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106661612A (zh) * | 2014-01-27 | 2017-05-10 | 通用医疗公司 | 制备用于测序的核酸的方法 |
CN107002080A (zh) * | 2014-12-18 | 2017-08-01 | 深圳华大基因研究院 | 一种基于多重pcr的目标区域富集方法和试剂 |
CN107709557A (zh) * | 2015-04-15 | 2018-02-16 | 通用医疗公司 | 基于lna的突变型富集下一代测序测定 |
WO2017024784A1 (zh) * | 2015-08-10 | 2017-02-16 | 北京吉因加科技有限公司 | 一种血浆中游离的目标dna低频突变富集测序方法 |
CN107475403A (zh) * | 2017-09-14 | 2017-12-15 | 深圳因合生物科技有限公司 | 从外周血游离dna中检测循环肿瘤dna的方法、试剂盒及其测序结果的分析方法 |
Non-Patent Citations (3)
Title |
---|
"Circulating Tumor DNA in Non–Small-Cell Lung Cancer:A Primer for the Clinician";Aditi P. Singh et al.;《JCO Precision Oncology》;20171231;第1-13页 * |
"Highly sensitive detection of ESR1 mutations in cell-free DNA from patients with metastatic breast cancer using molecular barcode sequencing";Nanae Masunaga et al.;《Breast Cancer Res Treat》;20170913;第1-10页 * |
"循环肿瘤DNA与肿瘤精准治疗";高芃等;《实用医院临床杂志》;20160531;第13卷(第3期);第1-6页 * |
Also Published As
Publication number | Publication date |
---|---|
CN110241209A (zh) | 2019-09-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US11667959B2 (en) | Systems and methods to detect rare mutations and copy number variation | |
JP6664025B2 (ja) | まれな変異およびコピー数多型を検出するためのシステムおよび方法 | |
ES2929281T3 (es) | Métodos para el enriquecimiento de secuencias de ácidos nucleicos dirigidos con aplicaciones para la secuenciación de ácidos nucleicos con corrección de errores | |
US10894974B2 (en) | Systems and methods to detect rare mutations and copy number variation | |
EP3763825B1 (en) | High multiplex pcr with molecular barcoding | |
CN104995309B (zh) | 多重核酸检测方法 | |
CN110785490A (zh) | 用于检测基因组变异和dna甲基化状态的组合物和方法 | |
JP2018521675A (ja) | 単一プローブプライマー伸長による標的濃縮 | |
WO2014149134A2 (en) | Systems and methods to detect rare mutations and copy number variation | |
EP3122894A1 (en) | Accurate detection of rare genetic variants in next generation sequencing | |
KR102648647B1 (ko) | 짧은 호모폴리머릭 반복서열의 개선된 검출법 | |
US11913065B2 (en) | Systems and methods to detect rare mutations and copy number variation | |
CN108504649B (zh) | 编码pcr二代测序建库方法、试剂盒及检测方法 | |
CN110241209B (zh) | 一种引物、试剂盒及用途 | |
WO2023159817A1 (zh) | 一种基因诊断探针及其应用 | |
US20220389489A1 (en) | Systems and methods to detect rare mutations and copy number variation | |
KR20220164753A (ko) | 플로팅 바코드 | |
CN108103064B (zh) | 长链非编码rna及其应用 | |
CN110546272B (zh) | 将衔接子附接至样品核酸的方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20200119 Address after: No. 2, Zhongke Road, Zhanmao street, Putuo District, Zhoushan City, Zhejiang Province Applicant after: Zhejiang grade Gene Technology Co.,Ltd. Address before: Dunlop apartment, Austin, Texas 11216, USA Applicant before: Lin Yunfu |
|
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20230414 Address after: Room 1001-75, Block B, Donggang Wealth Center, No. 211 Qilin Street, Donggang Street, Putuo District, Zhoushan City, Zhejiang Province, 316104 Patentee after: Zhejiang grade Gene Technology Co.,Ltd. Patentee after: Zhoushan Grade Medical Laboratory Co.,Ltd. Address before: No. 2, Zhongke Road, Zhanmao Street, Putuo District, Zhoushan City, Zhejiang Province, 316104 Patentee before: Zhejiang grade Gene Technology Co.,Ltd. |