JP2019506163A5 - - Google Patents

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Publication number
JP2019506163A5
JP2019506163A5 JP2018539361A JP2018539361A JP2019506163A5 JP 2019506163 A5 JP2019506163 A5 JP 2019506163A5 JP 2018539361 A JP2018539361 A JP 2018539361A JP 2018539361 A JP2018539361 A JP 2018539361A JP 2019506163 A5 JP2019506163 A5 JP 2019506163A5
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JP
Japan
Prior art keywords
fragment
intein
split intein
compound
split
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JP2018539361A
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English (en)
Japanese (ja)
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JP7290305B2 (ja
JP2019506163A (ja
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Priority claimed from PCT/US2017/015455 external-priority patent/WO2017132580A2/en
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Publication of JP2019506163A5 publication Critical patent/JP2019506163A5/ja
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JP2018539361A 2016-01-29 2017-01-27 例外的なスプライシング活性を有するスプリットインテイン Active JP7290305B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662288661P 2016-01-29 2016-01-29
US62/288,661 2016-01-29
PCT/US2017/015455 WO2017132580A2 (en) 2016-01-29 2017-01-27 Split inteins with exceptional splicing activity

Publications (3)

Publication Number Publication Date
JP2019506163A JP2019506163A (ja) 2019-03-07
JP2019506163A5 true JP2019506163A5 (enExample) 2020-03-05
JP7290305B2 JP7290305B2 (ja) 2023-06-13

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Family Applications (1)

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JP2018539361A Active JP7290305B2 (ja) 2016-01-29 2017-01-27 例外的なスプライシング活性を有するスプリットインテイン

Country Status (14)

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US (2) US11142550B2 (enExample)
EP (2) EP3408292B1 (enExample)
JP (1) JP7290305B2 (enExample)
CN (1) CN108884154A (enExample)
AU (1) AU2017211395B2 (enExample)
CA (1) CA3051195A1 (enExample)
DK (1) DK3408292T3 (enExample)
ES (1) ES2949163T3 (enExample)
FI (1) FI3408292T3 (enExample)
HU (1) HUE062276T2 (enExample)
PL (1) PL3408292T3 (enExample)
PT (1) PT3408292T (enExample)
SI (1) SI3408292T1 (enExample)
WO (1) WO2017132580A2 (enExample)

Families Citing this family (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3613852A3 (en) 2011-07-22 2020-04-22 President and Fellows of Harvard College Evaluation and improvement of nuclease cleavage specificity
DK2986635T3 (en) 2013-04-18 2019-01-28 Fond Telethon EFFECTIVE DELIVERY OF BIG GENES THROUGH DUAL-AAV VECTORS
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
US11053481B2 (en) 2013-12-12 2021-07-06 President And Fellows Of Harvard College Fusions of Cas9 domains and nucleic acid-editing domains
AU2015298571B2 (en) 2014-07-30 2020-09-03 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
US11319528B2 (en) 2015-07-13 2022-05-03 University Of Utah Research Foundation Methods of making red blood cells and platelets in vitro and uses thereof
US12043852B2 (en) 2015-10-23 2024-07-23 President And Fellows Of Harvard College Evolved Cas9 proteins for gene editing
JP7290305B2 (ja) * 2016-01-29 2023-06-13 ザ・トラスティーズ・オブ・プリンストン・ユニバーシティ 例外的なスプライシング活性を有するスプリットインテイン
GB2568182A (en) 2016-08-03 2019-05-08 Harvard College Adenosine nucleobase editors and uses thereof
WO2018031683A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
GB2573062A (en) * 2016-10-14 2019-10-23 Harvard College AAV delivery of nucleobase editors
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
US11898179B2 (en) 2017-03-09 2024-02-13 President And Fellows Of Harvard College Suppression of pain by gene editing
WO2018165631A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Cancer vaccine
CN110914310A (zh) 2017-03-10 2020-03-24 哈佛大学的校长及成员们 胞嘧啶至鸟嘌呤碱基编辑器
US11268082B2 (en) 2017-03-23 2022-03-08 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable DNA binding proteins
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
JP2020534795A (ja) 2017-07-28 2020-12-03 プレジデント アンド フェローズ オブ ハーバード カレッジ ファージによって支援される連続的進化(pace)を用いて塩基編集因子を進化させるための方法および組成物
WO2019139645A2 (en) 2017-08-30 2019-07-18 President And Fellows Of Harvard College High efficiency base editors comprising gam
CA3082251A1 (en) 2017-10-16 2019-04-25 The Broad Institute, Inc. Uses of adenosine base editors
US12406749B2 (en) 2017-12-15 2025-09-02 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
US20210277379A1 (en) 2018-08-03 2021-09-09 Beam Therapeutics Inc. Multi-effector nucleobase editors and methods of using same to modify a nucleic acid target sequence
US11530245B2 (en) * 2018-08-31 2022-12-20 Washington University Split intein mediated polymerization and production of mussel foot adhesive protein materials
US12454694B2 (en) 2018-09-07 2025-10-28 Beam Therapeutics Inc. Compositions and methods for improving base editing
CN113348249B (zh) 2018-10-15 2025-09-23 特莱索恩基金会 内蛋白及其用途
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
LU101118B1 (en) * 2019-02-06 2020-08-06 Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Method for detecting a specific splice event of a gene of interest
AU2020240109A1 (en) 2019-03-19 2021-09-30 President And Fellows Of Harvard College Methods and compositions for editing nucleotide sequences
WO2020214842A1 (en) 2019-04-17 2020-10-22 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
CN110714020B (zh) * 2019-05-22 2021-08-03 北京理工大学 一种高效简便纯化蛋白的方法
CA3147875A1 (en) 2019-07-19 2021-01-28 Flagship Pioneering Innovations Vi, Llc Recombinase compositions and methods of use
AU2019463636A1 (en) * 2019-08-28 2022-03-17 Albert Einstein College Of Medicine Atypical split inteins and uses thereof
CN114450292B (zh) * 2019-09-09 2024-07-23 武汉友芝友生物制药股份有限公司 断裂型内含肽、使用其的重组多肽的制备方法
CA3153342A1 (en) * 2019-10-01 2021-04-08 Tara DEANS Methods of engineering platelets for targeting circulating tumor cells
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
GB201917046D0 (en) * 2019-11-22 2020-01-08 Ge Healthcare Bioprocess R&D Ab Improved protein production
IL296024A (en) 2020-03-04 2022-10-01 Flagship Pioneering Innovations Vi Llc Methods and compositions for modulating a genome
CN111235174A (zh) * 2020-03-11 2020-06-05 梦芊科技知识产权有限公司 利用枯草芽孢杆菌和核酸内切酶制备人碱性成纤维细胞生长因子
EP3885440A1 (en) * 2020-03-26 2021-09-29 Splicebio, S.L. Split inteins and their uses
BR112022022603A2 (pt) 2020-05-08 2023-01-17 Broad Inst Inc Métodos e composições para edição simultânea de ambas as fitas de sequência alvo de nucleotídeos de fita dupla
US20230227508A1 (en) 2020-06-12 2023-07-20 Massachusetts Institute Of Technology Split intein-based selection for peptide binders
CN117355536A (zh) * 2021-05-12 2024-01-05 思拓凡生物工艺研发有限公司 改进的蛋白质纯化
CN113735941B (zh) * 2021-08-13 2024-01-30 江南大学 一种蛋白纯化方法及其应用
KR102663243B1 (ko) * 2021-08-30 2024-05-03 국립순천대학교산학협력단 트립신 특이적 형광 프로브 및 이의 용도
MX2024002927A (es) 2021-09-08 2024-05-29 Flagship Pioneering Innovations Vi Llc Metodos y composiciones para modular un genoma.
WO2023081714A1 (en) * 2021-11-02 2023-05-11 Cornell University Intein systems and uses thereof
WO2023229029A1 (ja) * 2022-05-26 2023-11-30 国立大学法人山形大学 ヘテロダイマータンパク質の製造方法、ダイマータンパク質、モノマータンパク質、および標的反応性のヘテロダイマータンパク質のスクリーニング方法
CA3267375A1 (en) * 2022-09-26 2024-04-04 Beam Therapeutics Inc. SYNTHETIC POLYPEPTIDES AND THEIR USES
CN120641436A (zh) * 2022-11-01 2025-09-12 纪念斯隆-凯特琳癌症中心 基于内含肽的分选系统和模块化嵌合多肽
WO2024170051A1 (en) * 2023-02-13 2024-08-22 Kutzner Christoph Fusion polypeptides for manufacturing of cyclic peptides
WO2024258785A1 (en) 2023-06-11 2024-12-19 Regeneron Pharmaceuticals, Inc. Circularized antibody molecules
WO2025051758A1 (en) 2023-09-04 2025-03-13 Splicebio, Sl Use of split intein for the treatment of scn1a associated disease
WO2025153705A1 (en) 2024-01-18 2025-07-24 Splicebio, Sl Use of split intein for the treatment of myo7a-associated disease
WO2025194124A1 (en) 2024-03-14 2025-09-18 Tessera Therapeutics, Inc. Modified st1cas9 guide nucleic acids

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102096534B1 (ko) 2011-09-28 2020-04-03 에라 바이오테크, 에스.에이. 분할된 인테인 및 그의 이용
US20150344549A1 (en) 2012-06-27 2015-12-03 The Trustees Of Princeton University Split inteins, conjugates and uses thereof
US10087213B2 (en) * 2013-01-11 2018-10-02 The Texas A&M University System Intein mediated purification of protein
JP7290305B2 (ja) * 2016-01-29 2023-06-13 ザ・トラスティーズ・オブ・プリンストン・ユニバーシティ 例外的なスプライシング活性を有するスプリットインテイン

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