JP7290305B2 - 例外的なスプライシング活性を有するスプリットインテイン - Google Patents

例外的なスプライシング活性を有するスプリットインテイン Download PDF

Info

Publication number
JP7290305B2
JP7290305B2 JP2018539361A JP2018539361A JP7290305B2 JP 7290305 B2 JP7290305 B2 JP 7290305B2 JP 2018539361 A JP2018539361 A JP 2018539361A JP 2018539361 A JP2018539361 A JP 2018539361A JP 7290305 B2 JP7290305 B2 JP 7290305B2
Authority
JP
Japan
Prior art keywords
seq
fragment
intein
split intein
residue
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2018539361A
Other languages
English (en)
Japanese (ja)
Other versions
JP2019506163A (ja
JP2019506163A5 (enExample
Inventor
ミューア、トム、ダブリュー
スティーブンス、アダム、ジェイ
シャー、ニール、エイチ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Princeton University
Original Assignee
Princeton University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Princeton University filed Critical Princeton University
Publication of JP2019506163A publication Critical patent/JP2019506163A/ja
Publication of JP2019506163A5 publication Critical patent/JP2019506163A5/ja
Application granted granted Critical
Publication of JP7290305B2 publication Critical patent/JP7290305B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2851—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the lectin superfamily, e.g. CD23, CD72
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
    • C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
JP2018539361A 2016-01-29 2017-01-27 例外的なスプライシング活性を有するスプリットインテイン Active JP7290305B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662288661P 2016-01-29 2016-01-29
US62/288,661 2016-01-29
PCT/US2017/015455 WO2017132580A2 (en) 2016-01-29 2017-01-27 Split inteins with exceptional splicing activity

Publications (3)

Publication Number Publication Date
JP2019506163A JP2019506163A (ja) 2019-03-07
JP2019506163A5 JP2019506163A5 (enExample) 2020-03-05
JP7290305B2 true JP7290305B2 (ja) 2023-06-13

Family

ID=59398921

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2018539361A Active JP7290305B2 (ja) 2016-01-29 2017-01-27 例外的なスプライシング活性を有するスプリットインテイン

Country Status (14)

Country Link
US (2) US11142550B2 (enExample)
EP (2) EP3408292B1 (enExample)
JP (1) JP7290305B2 (enExample)
CN (1) CN108884154A (enExample)
AU (1) AU2017211395B2 (enExample)
CA (1) CA3051195A1 (enExample)
DK (1) DK3408292T3 (enExample)
ES (1) ES2949163T3 (enExample)
FI (1) FI3408292T3 (enExample)
HU (1) HUE062276T2 (enExample)
PL (1) PL3408292T3 (enExample)
PT (1) PT3408292T (enExample)
SI (1) SI3408292T1 (enExample)
WO (1) WO2017132580A2 (enExample)

Families Citing this family (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2734621B1 (en) 2011-07-22 2019-09-04 President and Fellows of Harvard College Evaluation and improvement of nuclease cleavage specificity
EP2986635B1 (en) 2013-04-18 2018-10-03 Fondazione Telethon Effective delivery of large genes by dual aav vectors
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9340800B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College Extended DNA-sensing GRNAS
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9840699B2 (en) 2013-12-12 2017-12-12 President And Fellows Of Harvard College Methods for nucleic acid editing
WO2016022363A2 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
WO2017011550A1 (en) 2015-07-13 2017-01-19 University Of Utah Research Foundation Methods of making red blood cells and platelets in vitro and uses thereof
IL258821B (en) 2015-10-23 2022-07-01 Harvard College Nucleobase editors and their uses
PL3408292T3 (pl) * 2016-01-29 2023-10-02 The Trustees Of Princeton University Podzielone inteiny o wyjątkowej aktywności splicingu
KR20250103795A (ko) 2016-08-03 2025-07-07 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 아데노신 핵염기 편집제 및 그의 용도
CA3033327A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
JP7588390B2 (ja) 2016-10-14 2024-11-22 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸塩基エディターのａａｖ送達
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
CN110662556A (zh) 2017-03-09 2020-01-07 哈佛大学的校长及成员们 癌症疫苗
WO2018165504A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Suppression of pain by gene editing
JP2020510439A (ja) 2017-03-10 2020-04-09 プレジデント アンド フェローズ オブ ハーバード カレッジ シトシンからグアニンへの塩基編集因子
BR112019019655A2 (pt) 2017-03-23 2020-04-22 Harvard College editores de nucleobase que compreendem proteínas de ligação a dna programáveis por ácido nucleico
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
JP2020534795A (ja) 2017-07-28 2020-12-03 プレジデント アンド フェローズ オブ ハーバード カレッジ ファージによって支援される連続的進化（ｐａｃｅ）を用いて塩基編集因子を進化させるための方法および組成物
US11319532B2 (en) 2017-08-30 2022-05-03 President And Fellows Of Harvard College High efficiency base editors comprising Gam
KR20250107288A (ko) 2017-10-16 2025-07-11 더 브로드 인스티튜트, 인코퍼레이티드 아데노신 염기 편집제의 용도
WO2019118949A1 (en) 2017-12-15 2019-06-20 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
WO2019226953A1 (en) 2018-05-23 2019-11-28 The Broad Institute, Inc. Base editors and uses thereof
KR102894715B1 (ko) 2018-08-03 2025-12-03 빔 테라퓨틱스, 인크. 핵산 표적 서열을 변형시키기 위한 다중-이펙터 핵염기 편집기 및 이를 이용하는 방법
US11530245B2 (en) * 2018-08-31 2022-12-20 Washington University Split intein mediated polymerization and production of mussel foot adhesive protein materials
WO2020051562A2 (en) 2018-09-07 2020-03-12 Beam Therapeutics Inc. Compositions and methods for improving base editing
IL320368A (en) * 2018-10-15 2025-06-01 Fond Telethon Ets Intein proteins and their uses
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
LU101118B1 (en) * 2019-02-06 2020-08-06 Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Method for detecting a specific splice event of a gene of interest
EP3942042A1 (en) 2019-03-19 2022-01-26 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
EP3956349A1 (en) 2019-04-17 2022-02-23 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
CN110714020B (zh) * 2019-05-22 2021-08-03 北京理工大学 一种高效简便纯化蛋白的方法
WO2021016075A1 (en) 2019-07-19 2021-01-28 Flagship Pioneering Innovations Vi, Llc Recombinase compositions and methods of use
WO2021040703A1 (en) * 2019-08-28 2021-03-04 The Trustees Of Princeton University Atypical split inteins and uses thereof
WO2021047559A1 (zh) * 2019-09-09 2021-03-18 武汉友芝友生物制药有限公司 一种断裂型内含肽、使用其的重组多肽的制备方法
KR20220070481A (ko) * 2019-10-01 2022-05-31 유타대학연구재단 순환 종양 세포를 표적화하기 위한 혈소판 조작 방법
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
GB201917046D0 (en) * 2019-11-22 2020-01-08 Ge Healthcare Bioprocess R&D Ab Improved protein production
MX2022010991A (es) 2020-03-04 2023-02-09 Flagship Pioneering Innovations Vi Llc Metodos y composiciones para modular un genoma.
CN111235174A (zh) * 2020-03-11 2020-06-05 梦芊科技知识产权有限公司 利用枯草芽孢杆菌和核酸内切酶制备人碱性成纤维细胞生长因子
EP3885440A1 (en) * 2020-03-26 2021-09-29 Splicebio, S.L. Split inteins and their uses
WO2021226558A1 (en) 2020-05-08 2021-11-11 The Broad Institute, Inc. Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence
US20230227508A1 (en) 2020-06-12 2023-07-20 Massachusetts Institute Of Technology Split intein-based selection for peptide binders
US20250282815A1 (en) * 2021-05-12 2025-09-11 Cytiva Bioprocess R&D Ab Improved protein purification
CN113735941B (zh) * 2021-08-13 2024-01-30 江南大学 一种蛋白纯化方法及其应用
KR102663243B1 (ko) * 2021-08-30 2024-05-03 국립순천대학교산학협력단 트립신 특이적 형광 프로브 및 이의 용도
JP2024533311A (ja) 2021-09-08 2024-09-12 フラッグシップ パイオニアリング イノベーションズ シックス，エルエルシー ゲノムを調節するための方法及び組成物
WO2023081714A1 (en) * 2021-11-02 2023-05-11 Cornell University Intein systems and uses thereof
CN119630796A (zh) * 2022-05-26 2025-03-14 国立大学法人山形大学 异二聚体蛋白质的制造方法、二聚体蛋白质、单体蛋白质，以及靶标反应性异二聚体蛋白质的筛选方法
EP4594486A2 (en) * 2022-09-26 2025-08-06 Beam Therapeutics Inc. Synthetic polypeptides and uses thereof
WO2024097763A1 (en) * 2022-11-01 2024-05-10 Memorial Sloan-Kettering Cancer Center Intein-based sorting system and modular chimeric polypeptides
WO2024170051A1 (en) * 2023-02-13 2024-08-22 Kutzner Christoph Fusion polypeptides for manufacturing of cyclic peptides
WO2024258785A1 (en) 2023-06-11 2024-12-19 Regeneron Pharmaceuticals, Inc. Circularized antibody molecules
WO2025051758A1 (en) 2023-09-04 2025-03-13 Splicebio, Sl Use of split intein for the treatment of scn1a associated disease
WO2025153705A1 (en) 2024-01-18 2025-07-24 Splicebio, Sl Use of split intein for the treatment of myo7a-associated disease
WO2025194124A1 (en) 2024-03-14 2025-09-18 Tessera Therapeutics, Inc. Modified st1cas9 guide nucleic acids

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014528720A (ja) 2011-09-28 2014-10-30 エラ、ビオテック、ソシエダッド、アノニマＥｒａ Ｂｉｏｔｅｃｈ， Ｓ．Ａ． スプリットインテインおよびその使用
JP2015522020A (ja) 2012-06-27 2015-08-03 ザ・トラスティーズ・オブ・プリンストン・ユニバーシティＴｈｅ Ｔｒｕｓｔｅｅｓ Ｏｆ Ｐｒｉｎｃｅｔｏｎ Ｕｎｉｖｅｒｓｉｔｙ スプリットインテイン、複合体およびそれらの使用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10087213B2 (en) * 2013-01-11 2018-10-02 The Texas A&M University System Intein mediated purification of protein
PL3408292T3 (pl) * 2016-01-29 2023-10-02 The Trustees Of Princeton University Podzielone inteiny o wyjątkowej aktywności splicingu

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014528720A (ja) 2011-09-28 2014-10-30 エラ、ビオテック、ソシエダッド、アノニマＥｒａ Ｂｉｏｔｅｃｈ， Ｓ．Ａ． スプリットインテインおよびその使用
JP2015522020A (ja) 2012-06-27 2015-08-03 ザ・トラスティーズ・オブ・プリンストン・ユニバーシティＴｈｅ Ｔｒｕｓｔｅｅｓ Ｏｆ Ｐｒｉｎｃｅｔｏｎ Ｕｎｉｖｅｒｓｉｔｙ スプリットインテイン、複合体およびそれらの使用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Shah, N. H. et al.，Ultrafast protein splicing is common among cyanobacterial split inteins: implications for protein engineering，J. Am. Chem. Soc.，2012年，Vol. 134(28)，pp. 11338-11341, Supporting information
山崎俊夫 ほか，インテインを利用した蛋白質フラグメントのライゲーション，生物物理，1999年，Vol. 39，pp. 182-184

Also Published As

Publication number Publication date
FI3408292T3 (fi) 2023-06-30
EP3408292A4 (en) 2019-09-18
CA3051195A1 (en) 2017-08-03
JP2019506163A (ja) 2019-03-07
PL3408292T3 (pl) 2023-10-02
EP4234689A2 (en) 2023-08-30
US20210371467A1 (en) 2021-12-02
AU2017211395B2 (en) 2024-04-18
DK3408292T3 (da) 2023-06-12
EP3408292A2 (en) 2018-12-05
WO2017132580A3 (en) 2017-09-14
SI3408292T1 (sl) 2023-09-29
EP3408292B1 (en) 2023-04-26
EP4234689A3 (en) 2023-09-13
CN108884154A (zh) 2018-11-23
US11142550B2 (en) 2021-10-12
HUE062276T2 (hu) 2023-10-28
PT3408292T (pt) 2023-07-19
US12297472B2 (en) 2025-05-13
US20200055900A1 (en) 2020-02-20
WO2017132580A2 (en) 2017-08-03
ES2949163T3 (es) 2023-09-26
AU2017211395A1 (en) 2018-09-06

Similar Documents

Publication Publication Date Title
US12297472B2 (en) Split inteins with exceptional splicing activity
US12054541B2 (en) Split inteins, conjugates and uses thereof
ES2880336T3 (es) Métodos y productos para la síntesis de proteínas de fusión
Johnson et al. Towards the total chemical synthesis of integral membrane proteins: a general method for the synthesis of hydrophobic peptide-αthioester building blocks
CN109790205B (zh) 酶促肽连接的方法
EP2075336B1 (en) Purification of recombinant proteins fused to multiple epitopes
CA3087218C (en) Biological synthesis of amino acid chains for preparation of peptides and proteins
EP3750911A1 (en) Cysteine-free inteins
CN111073925B (zh) 一种基于无序蛋白偶联酶的高效多肽-多肽偶联系统和方法
CN117801123B (zh) 沃索利肽可溶性中间体、中间体制备方法及沃索利肽的制备方法
HK40098890A (en) Split inteins with exceptional splicing activity
HK40001246B (en) Split inteins with exceptional splicing activity
HK40001246A (en) Split inteins with exceptional splicing activity
KR20210079235A (ko) Whep 도메인 융합에 의한 목적 단백질의 수용성 증진 방법
Adelakun et al. Analyzing protein posttranslational modifications using enzyme-catalyzed expressed protein ligation
Stevens et al. Supporting Information for: Design of a Split Intein with Exceptional Protein Splicing Activity
Khairil Extending the scope of covalent peptide-protein conjugation: from purification to expanded nanoassembly
Cappellini An evaluation of an innovative technological platform for straightforward purification of recombinant proteins

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200123

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20200123

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20210302

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20210531

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210802

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20210803

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20211221

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20220322

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220413

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20220830

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20221226

C60 Trial request (containing other claim documents, opposition documents)

Free format text: JAPANESE INTERMEDIATE CODE: C60

Effective date: 20221226

C11 Written invitation by the commissioner to file amendments

Free format text: JAPANESE INTERMEDIATE CODE: C11

Effective date: 20230110

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20230307

C21 Notice of transfer of a case for reconsideration by examiners before appeal proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C21

Effective date: 20230314

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20230502

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20230525

R150 Certificate of patent or registration of utility model

Ref document number: 7290305

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150