JP2018527934A5 - - Google Patents
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- JP2018527934A5 JP2018527934A5 JP2018512365A JP2018512365A JP2018527934A5 JP 2018527934 A5 JP2018527934 A5 JP 2018527934A5 JP 2018512365 A JP2018512365 A JP 2018512365A JP 2018512365 A JP2018512365 A JP 2018512365A JP 2018527934 A5 JP2018527934 A5 JP 2018527934A5
- Authority
- JP
- Japan
- Prior art keywords
- plant
- asparagine
- curing
- tobacco
- plants
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP15184528 | 2015-09-09 | ||
| EP15184528.6 | 2015-09-09 | ||
| PCT/EP2016/070972 WO2017042162A1 (en) | 2015-09-09 | 2016-09-06 | Plants with reduced asparagine content |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018527934A JP2018527934A (ja) | 2018-09-27 |
| JP2018527934A5 true JP2018527934A5 (enExample) | 2019-06-20 |
| JP6810134B2 JP6810134B2 (ja) | 2021-01-06 |
Family
ID=54105683
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018512365A Active JP6810134B2 (ja) | 2015-09-09 | 2016-09-06 | アスパラギン含有量を低減させた植物体 |
Country Status (21)
| Country | Link |
|---|---|
| US (1) | US10883114B2 (enExample) |
| EP (1) | EP3346826B8 (enExample) |
| JP (1) | JP6810134B2 (enExample) |
| KR (1) | KR102207550B1 (enExample) |
| CN (1) | CN107920489A (enExample) |
| AR (1) | AR105968A1 (enExample) |
| AU (1) | AU2016318423B2 (enExample) |
| BR (1) | BR112018002914A2 (enExample) |
| CA (1) | CA2998286C (enExample) |
| ES (1) | ES3014919T3 (enExample) |
| HK (1) | HK1255341A1 (enExample) |
| IL (1) | IL257305B (enExample) |
| MX (1) | MX392152B (enExample) |
| MY (1) | MY187046A (enExample) |
| NZ (1) | NZ738897A (enExample) |
| PH (1) | PH12017502292A1 (enExample) |
| PL (1) | PL3346826T3 (enExample) |
| RU (1) | RU2742725C2 (enExample) |
| UA (1) | UA125478C2 (enExample) |
| WO (1) | WO2017042162A1 (enExample) |
| ZA (1) | ZA201708411B (enExample) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111801423A (zh) | 2018-03-28 | 2020-10-20 | 菲利普莫里斯生产公司 | 调节植物中的氨基酸含量 |
| BR112020017430A2 (pt) | 2018-03-28 | 2021-01-19 | Philip Morris Products S.A. | Modulação do teor de açúcar redutor em uma planta |
| CN113271768B (zh) | 2018-12-30 | 2024-02-06 | 菲利普莫里斯生产公司 | 经由硝酸盐还原酶的突变来调节植物中的硝酸盐水平 |
| US12419264B2 (en) * | 2019-10-01 | 2025-09-23 | Philip Morris Products S.A. | Modulating sugar and amino acid content in a plant (SULTR3) |
| BR112022005552A2 (pt) | 2019-10-01 | 2022-06-28 | Philip Morris Products Sa | Modulação da redução do teor de açúcar em uma planta (inv) |
| CN112931182B (zh) * | 2021-01-29 | 2022-06-07 | 四川农业大学 | 一种提高小麦染色体重组频率的方法 |
| WO2023036691A1 (en) | 2021-09-10 | 2023-03-16 | Philip Morris Products S.A. | Modulating alkaloid profiles in nicotiana tabacum |
| WO2023117661A1 (en) | 2021-12-20 | 2023-06-29 | Philip Morris Products S.A. | Increasing anatabine in tobacco leaf by regulating methyl putrescine oxidase |
| WO2023117701A1 (en) | 2021-12-21 | 2023-06-29 | Philip Morris Products S.A. | Modulation of nicotine production by alteration of nicotinamidase expression or function in plants |
| CN114766359B (zh) * | 2022-04-22 | 2023-03-31 | 华中农业大学 | 一种试管苗支架及其在柑橘茎尖微芽嫁接脱毒中的应用 |
| JP2025533986A (ja) | 2022-10-13 | 2025-10-09 | フィリップ・モーリス・プロダクツ・ソシエテ・アノニム | Ntp2を調節することによって葉バイオマスおよび窒素利用効率を増加させる |
| CN120476136A (zh) | 2023-02-02 | 2025-08-12 | 菲利普莫里斯生产公司 | 编码赖氨酸酮戊二酸还原酶的基因的调节 |
| KR20250139371A (ko) | 2023-02-02 | 2025-09-23 | 필립모리스 프로덕츠 에스.에이. | 당 수송체의 조절 |
| CN116574719B (zh) * | 2023-06-28 | 2024-06-25 | 四川农业大学 | AsMIPS1基因克隆、表达载体构建和应用 |
| WO2025003105A1 (en) | 2023-06-29 | 2025-01-02 | Philip Morris Products S.A. | Modulation of genes coding for glutamate dehydrogenase |
| CN119876252B (zh) * | 2025-03-27 | 2025-06-20 | 浙江农林大学 | 毛竹PeNRAMP5基因在降低水稻Cd积累增加Mn吸收的应用 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2013200664B2 (en) * | 2005-09-20 | 2015-04-30 | J.R. Simplot Company | Low acrylamide foods |
| PL2333076T3 (pl) | 2005-09-20 | 2015-08-31 | Simplot Co J R | Żywność o niskiej zawartości akrylamidu |
| RU2324737C1 (ru) * | 2006-10-18 | 2008-05-20 | Институт цитологии и генетики Сибирского отделения Российской академии наук (СО РАН) | Способ получения трансгенных растений табака с повышенным содержанием пролина |
| JP4953808B2 (ja) | 2006-12-29 | 2012-06-13 | フルタ電機株式会社 | 葉たばこの監視制御盤の制御盤により制御する乾燥方法 |
| CA2744827C (en) * | 2008-12-29 | 2019-10-15 | Evogene Ltd. | Polynucleotides, polypeptides encoded thereby, and methods of using same for increasing abiotic stress tolerance, biomass and/or yield in plants expressing same |
| KR101214690B1 (ko) * | 2010-10-15 | 2012-12-21 | 고려대학교 산학협력단 | 병저항성 관련 고추 아스파라진합성효소 유전자 CaAS1 및 이를 이용한 식물체의 병저항성 탐색 방법 |
| CN102121018A (zh) | 2010-12-22 | 2011-07-13 | 上海大学 | 一种具有天冬酰胺合成酶功能的基因、其编码蛋白及其应用 |
| BR122021009930B1 (pt) | 2011-09-21 | 2021-10-26 | Reynolds Technologies, Inc | Produto de tabaco |
| US8716571B2 (en) * | 2011-09-21 | 2014-05-06 | Reynolds Technologies, Inc. | Tobacco having reduced amounts of amino acids and methods for producing such lines |
| US20140033248A1 (en) | 2012-07-27 | 2014-01-30 | General Instrument Corporation | Including inserts in multimedia content |
| KR20150085846A (ko) | 2012-11-20 | 2015-07-24 | 제이.알.심프롯캄패니 | Tal-매개 전이 DNA 삽입방법 |
| US20140363561A1 (en) * | 2013-03-15 | 2014-12-11 | J.R. Simplot Company | Tal-mediated transfer dna insertion |
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