JP6810134B2 - アスパラギン含有量を低減させた植物体 - Google Patents

アスパラギン含有量を低減させた植物体 Download PDF

Info

Publication number
JP6810134B2
JP6810134B2 JP2018512365A JP2018512365A JP6810134B2 JP 6810134 B2 JP6810134 B2 JP 6810134B2 JP 2018512365 A JP2018512365 A JP 2018512365A JP 2018512365 A JP2018512365 A JP 2018512365A JP 6810134 B2 JP6810134 B2 JP 6810134B2
Authority
JP
Japan
Prior art keywords
seq
plant
tobacco plant
leaves
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2018512365A
Other languages
English (en)
Japanese (ja)
Other versions
JP2018527934A5 (enExample
JP2018527934A (ja
Inventor
ルシアン ボヴェ
ルシアン ボヴェ
Original Assignee
フィリップ・モーリス・プロダクツ・ソシエテ・アノニム
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by フィリップ・モーリス・プロダクツ・ソシエテ・アノニム filed Critical フィリップ・モーリス・プロダクツ・ソシエテ・アノニム
Publication of JP2018527934A publication Critical patent/JP2018527934A/ja
Publication of JP2018527934A5 publication Critical patent/JP2018527934A5/ja
Application granted granted Critical
Publication of JP6810134B2 publication Critical patent/JP6810134B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12Leaves
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • AHUMAN NECESSITIES
    • A24TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24BMANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18Treatment of tobacco products or tobacco substitutes
    • A24B15/20Biochemical treatment
    • AHUMAN NECESSITIES
    • A24TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24BMANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B3/00Preparing tobacco in the factory
    • A24B3/12Steaming, curing, or flavouring tobacco
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93Ligases (6)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y603/00Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/05Carbon-nitrogen ligases with glutamine as amido-N-donor (6.3.5)
    • C12Y603/05004Asparagine synthase (glutamine-hydrolyzing) (6.3.5.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Physiology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Manufacture Of Tobacco Products (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Fertilizers (AREA)
JP2018512365A 2015-09-09 2016-09-06 アスパラギン含有量を低減させた植物体 Active JP6810134B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP15184528 2015-09-09
EP15184528.6 2015-09-09
PCT/EP2016/070972 WO2017042162A1 (en) 2015-09-09 2016-09-06 Plants with reduced asparagine content

Publications (3)

Publication Number Publication Date
JP2018527934A JP2018527934A (ja) 2018-09-27
JP2018527934A5 JP2018527934A5 (enExample) 2019-06-20
JP6810134B2 true JP6810134B2 (ja) 2021-01-06

Family

ID=54105683

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2018512365A Active JP6810134B2 (ja) 2015-09-09 2016-09-06 アスパラギン含有量を低減させた植物体

Country Status (21)

Country Link
US (1) US10883114B2 (enExample)
EP (1) EP3346826B8 (enExample)
JP (1) JP6810134B2 (enExample)
KR (1) KR102207550B1 (enExample)
CN (1) CN107920489A (enExample)
AR (1) AR105968A1 (enExample)
AU (1) AU2016318423B2 (enExample)
BR (1) BR112018002914A2 (enExample)
CA (1) CA2998286C (enExample)
ES (1) ES3014919T3 (enExample)
HK (1) HK1255341A1 (enExample)
IL (1) IL257305B (enExample)
MX (1) MX392152B (enExample)
MY (1) MY187046A (enExample)
NZ (1) NZ738897A (enExample)
PH (1) PH12017502292A1 (enExample)
PL (1) PL3346826T3 (enExample)
RU (1) RU2742725C2 (enExample)
UA (1) UA125478C2 (enExample)
WO (1) WO2017042162A1 (enExample)
ZA (1) ZA201708411B (enExample)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111801423A (zh) 2018-03-28 2020-10-20 菲利普莫里斯生产公司 调节植物中的氨基酸含量
BR112020017430A2 (pt) 2018-03-28 2021-01-19 Philip Morris Products S.A. Modulação do teor de açúcar redutor em uma planta
CN113271768B (zh) 2018-12-30 2024-02-06 菲利普莫里斯生产公司 经由硝酸盐还原酶的突变来调节植物中的硝酸盐水平
US12419264B2 (en) * 2019-10-01 2025-09-23 Philip Morris Products S.A. Modulating sugar and amino acid content in a plant (SULTR3)
BR112022005552A2 (pt) 2019-10-01 2022-06-28 Philip Morris Products Sa Modulação da redução do teor de açúcar em uma planta (inv)
CN112931182B (zh) * 2021-01-29 2022-06-07 四川农业大学 一种提高小麦染色体重组频率的方法
WO2023036691A1 (en) 2021-09-10 2023-03-16 Philip Morris Products S.A. Modulating alkaloid profiles in nicotiana tabacum
WO2023117661A1 (en) 2021-12-20 2023-06-29 Philip Morris Products S.A. Increasing anatabine in tobacco leaf by regulating methyl putrescine oxidase
WO2023117701A1 (en) 2021-12-21 2023-06-29 Philip Morris Products S.A. Modulation of nicotine production by alteration of nicotinamidase expression or function in plants
CN114766359B (zh) * 2022-04-22 2023-03-31 华中农业大学 一种试管苗支架及其在柑橘茎尖微芽嫁接脱毒中的应用
JP2025533986A (ja) 2022-10-13 2025-10-09 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム Ntp2を調節することによって葉バイオマスおよび窒素利用効率を増加させる
CN120476136A (zh) 2023-02-02 2025-08-12 菲利普莫里斯生产公司 编码赖氨酸酮戊二酸还原酶的基因的调节
KR20250139371A (ko) 2023-02-02 2025-09-23 필립모리스 프로덕츠 에스.에이. 당 수송체의 조절
CN116574719B (zh) * 2023-06-28 2024-06-25 四川农业大学 AsMIPS1基因克隆、表达载体构建和应用
WO2025003105A1 (en) 2023-06-29 2025-01-02 Philip Morris Products S.A. Modulation of genes coding for glutamate dehydrogenase
CN119876252B (zh) * 2025-03-27 2025-06-20 浙江农林大学 毛竹PeNRAMP5基因在降低水稻Cd积累增加Mn吸收的应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2013200664B2 (en) * 2005-09-20 2015-04-30 J.R. Simplot Company Low acrylamide foods
PL2333076T3 (pl) 2005-09-20 2015-08-31 Simplot Co J R Żywność o niskiej zawartości akrylamidu
RU2324737C1 (ru) * 2006-10-18 2008-05-20 Институт цитологии и генетики Сибирского отделения Российской академии наук (СО РАН) Способ получения трансгенных растений табака с повышенным содержанием пролина
JP4953808B2 (ja) 2006-12-29 2012-06-13 フルタ電機株式会社 葉たばこの監視制御盤の制御盤により制御する乾燥方法
CA2744827C (en) * 2008-12-29 2019-10-15 Evogene Ltd. Polynucleotides, polypeptides encoded thereby, and methods of using same for increasing abiotic stress tolerance, biomass and/or yield in plants expressing same
KR101214690B1 (ko) * 2010-10-15 2012-12-21 고려대학교 산학협력단 병저항성 관련 고추 아스파라진합성효소 유전자 CaAS1 및 이를 이용한 식물체의 병저항성 탐색 방법
CN102121018A (zh) 2010-12-22 2011-07-13 上海大学 一种具有天冬酰胺合成酶功能的基因、其编码蛋白及其应用
BR122021009930B1 (pt) 2011-09-21 2021-10-26 Reynolds Technologies, Inc Produto de tabaco
US8716571B2 (en) * 2011-09-21 2014-05-06 Reynolds Technologies, Inc. Tobacco having reduced amounts of amino acids and methods for producing such lines
US20140033248A1 (en) 2012-07-27 2014-01-30 General Instrument Corporation Including inserts in multimedia content
KR20150085846A (ko) 2012-11-20 2015-07-24 제이.알.심프롯캄패니 Tal-매개 전이 DNA 삽입방법
US20140363561A1 (en) * 2013-03-15 2014-12-11 J.R. Simplot Company Tal-mediated transfer dna insertion

Also Published As

Publication number Publication date
RU2018112407A3 (enExample) 2020-02-25
CN107920489A (zh) 2018-04-17
KR102207550B1 (ko) 2021-01-27
AU2016318423B2 (en) 2022-01-20
ES3014919T3 (en) 2025-04-28
NZ738897A (en) 2019-10-25
US10883114B2 (en) 2021-01-05
CA2998286A1 (en) 2017-03-16
BR112018002914A2 (pt) 2018-11-06
CA2998286C (en) 2023-11-07
EP3346826B8 (en) 2025-04-16
UA125478C2 (uk) 2022-03-30
PH12017502292A1 (en) 2018-06-25
HK1255341A1 (zh) 2019-08-16
RU2742725C2 (ru) 2021-02-10
US20190169628A1 (en) 2019-06-06
IL257305B (en) 2022-06-01
MX392152B (es) 2025-03-21
EP3346826C0 (en) 2025-02-19
JP2018527934A (ja) 2018-09-27
AR105968A1 (es) 2017-11-29
RU2018112407A (ru) 2019-10-09
PL3346826T3 (pl) 2025-06-09
KR20180051530A (ko) 2018-05-16
EP3346826B1 (en) 2025-02-19
AU2016318423A1 (en) 2018-04-05
EP3346826A1 (en) 2018-07-18
MX2018002695A (es) 2018-04-13
WO2017042162A1 (en) 2017-03-16
ZA201708411B (en) 2019-01-30
MY187046A (en) 2021-08-27
IL257305A (en) 2018-03-29

Similar Documents

Publication Publication Date Title
JP6810134B2 (ja) アスパラギン含有量を低減させた植物体
JP6871158B2 (ja) 植物体におけるニコチンからノルニコチンへの変換の低減
JP6693747B2 (ja) 植物体中のたばこ特異的ニトロソアミンの低減
JP7000265B2 (ja) ニコチアナ・タバカムからのイソプロピルリンゴ酸シンターゼならびにその方法および使用
JP6225108B2 (ja) ニコチアナ・タバカムからのトレオニン合成酵素ならびにその方法および使用
US11685929B2 (en) Plants with shortened time to flowering
CN104080802B (zh) 在植物中调节β大马酮
JP2021045130A (ja) たばこプロテアーゼ遺伝子
US20170145431A1 (en) Modulation of nitrate content in plants
US20210115461A1 (en) Modulating amino acid content in a plant
JP2021519064A (ja) 植物体における還元糖含有量の調節
JP2025533986A (ja) Ntp2を調節することによって葉バイオマスおよび窒素利用効率を増加させる
BR112019010484B1 (pt) Método de diminuição do tempo para floração em uma planta de tabaco, método para a produção de material vegetal com um tempo reduzido para floração, material vegetal, e método de identificação de uma molécula que modula a expressão de um polinucleotídeo de tfl1 ou a atividade de um polipeptídeo de tfl1

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20180312

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20190128

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190426

A524 Written submission of copy of amendment under article 19 pct

Free format text: JAPANESE INTERMEDIATE CODE: A524

Effective date: 20190426

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20191007

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20191223

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200306

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20200622

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20201020

C60 Trial request (containing other claim documents, opposition documents)

Free format text: JAPANESE INTERMEDIATE CODE: C60

Effective date: 20201020

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20201027

C21 Notice of transfer of a case for reconsideration by examiners before appeal proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C21

Effective date: 20201029

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20201119

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20201210

R150 Certificate of patent or registration of utility model

Ref document number: 6810134

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250