AU2016318423B2 - Plants with reduced asparagine content - Google Patents

Plants with reduced asparagine content Download PDF

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Publication number
AU2016318423B2
AU2016318423B2 AU2016318423A AU2016318423A AU2016318423B2 AU 2016318423 B2 AU2016318423 B2 AU 2016318423B2 AU 2016318423 A AU2016318423 A AU 2016318423A AU 2016318423 A AU2016318423 A AU 2016318423A AU 2016318423 B2 AU2016318423 B2 AU 2016318423B2
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AU
Australia
Prior art keywords
plant
seq
cured
asparagine
mutant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2016318423A
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English (en)
Other versions
AU2016318423A1 (en
Inventor
Lucien Bovet
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Philip Morris Products SA
Original Assignee
Philip Morris Products SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Philip Morris Products SA filed Critical Philip Morris Products SA
Publication of AU2016318423A1 publication Critical patent/AU2016318423A1/en
Application granted granted Critical
Publication of AU2016318423B2 publication Critical patent/AU2016318423B2/en
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12—Leaves
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A24B15/20—Biochemical treatment
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B3/00—Preparing tobacco in the factory
    • A24B3/12—Steaming, curing, or flavouring tobacco
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/05—Carbon-nitrogen ligases with glutamine as amido-N-donor (6.3.5)
    • C12Y603/05004—Asparagine synthase (glutamine-hydrolyzing) (6.3.5.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Physiology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Manufacture Of Tobacco Products (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Fertilizers (AREA)
AU2016318423A 2015-09-09 2016-09-06 Plants with reduced asparagine content Ceased AU2016318423B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP15184528 2015-09-09
EP15184528.6 2015-09-09
PCT/EP2016/070972 WO2017042162A1 (en) 2015-09-09 2016-09-06 Plants with reduced asparagine content

Publications (2)

Publication Number Publication Date
AU2016318423A1 AU2016318423A1 (en) 2018-04-05
AU2016318423B2 true AU2016318423B2 (en) 2022-01-20

Family

ID=54105683

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2016318423A Ceased AU2016318423B2 (en) 2015-09-09 2016-09-06 Plants with reduced asparagine content

Country Status (21)

Country Link
US (1) US10883114B2 (enExample)
EP (1) EP3346826B8 (enExample)
JP (1) JP6810134B2 (enExample)
KR (1) KR102207550B1 (enExample)
CN (1) CN107920489A (enExample)
AR (1) AR105968A1 (enExample)
AU (1) AU2016318423B2 (enExample)
BR (1) BR112018002914A2 (enExample)
CA (1) CA2998286C (enExample)
ES (1) ES3014919T3 (enExample)
HK (1) HK1255341A1 (enExample)
IL (1) IL257305B (enExample)
MX (1) MX392152B (enExample)
MY (1) MY187046A (enExample)
NZ (1) NZ738897A (enExample)
PH (1) PH12017502292A1 (enExample)
PL (1) PL3346826T3 (enExample)
RU (1) RU2742725C2 (enExample)
UA (1) UA125478C2 (enExample)
WO (1) WO2017042162A1 (enExample)
ZA (1) ZA201708411B (enExample)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111801423A (zh) 2018-03-28 2020-10-20 菲利普莫里斯生产公司 调节植物中的氨基酸含量
BR112020017430A2 (pt) 2018-03-28 2021-01-19 Philip Morris Products S.A. Modulação do teor de açúcar redutor em uma planta
CN113271768B (zh) 2018-12-30 2024-02-06 菲利普莫里斯生产公司 经由硝酸盐还原酶的突变来调节植物中的硝酸盐水平
US12419264B2 (en) * 2019-10-01 2025-09-23 Philip Morris Products S.A. Modulating sugar and amino acid content in a plant (SULTR3)
BR112022005552A2 (pt) 2019-10-01 2022-06-28 Philip Morris Products Sa Modulação da redução do teor de açúcar em uma planta (inv)
CN112931182B (zh) * 2021-01-29 2022-06-07 四川农业大学 一种提高小麦染色体重组频率的方法
WO2023036691A1 (en) 2021-09-10 2023-03-16 Philip Morris Products S.A. Modulating alkaloid profiles in nicotiana tabacum
WO2023117661A1 (en) 2021-12-20 2023-06-29 Philip Morris Products S.A. Increasing anatabine in tobacco leaf by regulating methyl putrescine oxidase
WO2023117701A1 (en) 2021-12-21 2023-06-29 Philip Morris Products S.A. Modulation of nicotine production by alteration of nicotinamidase expression or function in plants
CN114766359B (zh) * 2022-04-22 2023-03-31 华中农业大学 一种试管苗支架及其在柑橘茎尖微芽嫁接脱毒中的应用
JP2025533986A (ja) 2022-10-13 2025-10-09 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム Ｎｔｐ２を調節することによって葉バイオマスおよび窒素利用効率を増加させる
CN120476136A (zh) 2023-02-02 2025-08-12 菲利普莫里斯生产公司 编码赖氨酸酮戊二酸还原酶的基因的调节
KR20250139371A (ko) 2023-02-02 2025-09-23 필립모리스 프로덕츠 에스.에이. 당 수송체의 조절
CN116574719B (zh) * 2023-06-28 2024-06-25 四川农业大学 AsMIPS1基因克隆、表达载体构建和应用
WO2025003105A1 (en) 2023-06-29 2025-01-02 Philip Morris Products S.A. Modulation of genes coding for glutamate dehydrogenase
CN119876252B (zh) * 2025-03-27 2025-06-20 浙江农林大学 毛竹PeNRAMP5基因在降低水稻Cd积累增加Mn吸收的应用

Citations (3)

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WO2007035752A2 (en) * 2005-09-20 2007-03-29 J.R. Simplot Company Low acrylamide foods
WO2013043853A1 (en) * 2011-09-21 2013-03-28 Reynolds Technologies, Inc. Tobacco having reduced amounts of amino acids and methods for producing such lines
WO2014144094A1 (en) * 2013-03-15 2014-09-18 J.R. Simplot Company Tal-mediated transfer dna insertion

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AU2013200664B2 (en) * 2005-09-20 2015-04-30 J.R. Simplot Company Low acrylamide foods
RU2324737C1 (ru) * 2006-10-18 2008-05-20 Институт цитологии и генетики Сибирского отделения Российской академии наук (СО РАН) Способ получения трансгенных растений табака с повышенным содержанием пролина
JP4953808B2 (ja) 2006-12-29 2012-06-13 フルタ電機株式会社 葉たばこの監視制御盤の制御盤により制御する乾燥方法
CA2744827C (en) * 2008-12-29 2019-10-15 Evogene Ltd. Polynucleotides, polypeptides encoded thereby, and methods of using same for increasing abiotic stress tolerance, biomass and/or yield in plants expressing same
KR101214690B1 (ko) * 2010-10-15 2012-12-21 고려대학교 산학협력단 병저항성 관련 고추 아스파라진합성효소 유전자 ＣａＡＳ１ 및 이를 이용한 식물체의 병저항성 탐색 방법
CN102121018A (zh) 2010-12-22 2011-07-13 上海大学 一种具有天冬酰胺合成酶功能的基因、其编码蛋白及其应用
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Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
WO2007035752A2 (en) * 2005-09-20 2007-03-29 J.R. Simplot Company Low acrylamide foods
WO2013043853A1 (en) * 2011-09-21 2013-03-28 Reynolds Technologies, Inc. Tobacco having reduced amounts of amino acids and methods for producing such lines
WO2014144094A1 (en) * 2013-03-15 2014-09-18 J.R. Simplot Company Tal-mediated transfer dna insertion

Non-Patent Citations (1)

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Also Published As

Publication number Publication date
RU2018112407A3 (enExample) 2020-02-25
CN107920489A (zh) 2018-04-17
KR102207550B1 (ko) 2021-01-27
ES3014919T3 (en) 2025-04-28
NZ738897A (en) 2019-10-25
US10883114B2 (en) 2021-01-05
CA2998286A1 (en) 2017-03-16
BR112018002914A2 (pt) 2018-11-06
CA2998286C (en) 2023-11-07
EP3346826B8 (en) 2025-04-16
UA125478C2 (uk) 2022-03-30
PH12017502292A1 (en) 2018-06-25
JP6810134B2 (ja) 2021-01-06
HK1255341A1 (zh) 2019-08-16
RU2742725C2 (ru) 2021-02-10
US20190169628A1 (en) 2019-06-06
IL257305B (en) 2022-06-01
MX392152B (es) 2025-03-21
EP3346826C0 (en) 2025-02-19
JP2018527934A (ja) 2018-09-27
AR105968A1 (es) 2017-11-29
RU2018112407A (ru) 2019-10-09
PL3346826T3 (pl) 2025-06-09
KR20180051530A (ko) 2018-05-16
EP3346826B1 (en) 2025-02-19
AU2016318423A1 (en) 2018-04-05
EP3346826A1 (en) 2018-07-18
MX2018002695A (es) 2018-04-13
WO2017042162A1 (en) 2017-03-16
ZA201708411B (en) 2019-01-30
MY187046A (en) 2021-08-27
IL257305A (en) 2018-03-29

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Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired