RU2742725C2 - Растения со сниженным содержанием аспарагина - Google Patents

Растения со сниженным содержанием аспарагина Download PDF

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Publication number
RU2742725C2
RU2742725C2 RU2018112407A RU2018112407A RU2742725C2 RU 2742725 C2 RU2742725 C2 RU 2742725C2 RU 2018112407 A RU2018112407 A RU 2018112407A RU 2018112407 A RU2018112407 A RU 2018112407A RU 2742725 C2 RU2742725 C2 RU 2742725C2
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Prior art keywords
plant
seq
asparagine
expression
treated
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RU2018112407A
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English (en)
Russian (ru)
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RU2018112407A3 (enExample
RU2018112407A (ru
Inventor
Люсьен БОВЕ
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Филип Моррис Продактс С.А.
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12Leaves
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • AHUMAN NECESSITIES
    • A24TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24BMANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18Treatment of tobacco products or tobacco substitutes
    • A24B15/20Biochemical treatment
    • AHUMAN NECESSITIES
    • A24TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24BMANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B3/00Preparing tobacco in the factory
    • A24B3/12Steaming, curing, or flavouring tobacco
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93Ligases (6)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y603/00Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/05Carbon-nitrogen ligases with glutamine as amido-N-donor (6.3.5)
    • C12Y603/05004Asparagine synthase (glutamine-hydrolyzing) (6.3.5.4)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Physiology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Manufacture Of Tobacco Products (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Fertilizers (AREA)
RU2018112407A 2015-09-09 2016-09-06 Растения со сниженным содержанием аспарагина RU2742725C2 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP15184528 2015-09-09
EP15184528.6 2015-09-09
PCT/EP2016/070972 WO2017042162A1 (en) 2015-09-09 2016-09-06 Plants with reduced asparagine content

Publications (3)

Publication Number Publication Date
RU2018112407A RU2018112407A (ru) 2019-10-09
RU2018112407A3 RU2018112407A3 (enExample) 2020-02-25
RU2742725C2 true RU2742725C2 (ru) 2021-02-10

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Application Number Title Priority Date Filing Date
RU2018112407A RU2742725C2 (ru) 2015-09-09 2016-09-06 Растения со сниженным содержанием аспарагина

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US (1) US10883114B2 (enExample)
EP (1) EP3346826B8 (enExample)
JP (1) JP6810134B2 (enExample)
KR (1) KR102207550B1 (enExample)
CN (1) CN107920489A (enExample)
AR (1) AR105968A1 (enExample)
AU (1) AU2016318423B2 (enExample)
BR (1) BR112018002914A2 (enExample)
CA (1) CA2998286C (enExample)
ES (1) ES3014919T3 (enExample)
HK (1) HK1255341A1 (enExample)
IL (1) IL257305B (enExample)
MX (1) MX392152B (enExample)
MY (1) MY187046A (enExample)
NZ (1) NZ738897A (enExample)
PH (1) PH12017502292A1 (enExample)
PL (1) PL3346826T3 (enExample)
RU (1) RU2742725C2 (enExample)
UA (1) UA125478C2 (enExample)
WO (1) WO2017042162A1 (enExample)
ZA (1) ZA201708411B (enExample)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111801423A (zh) 2018-03-28 2020-10-20 菲利普莫里斯生产公司 调节植物中的氨基酸含量
BR112020017430A2 (pt) 2018-03-28 2021-01-19 Philip Morris Products S.A. Modulação do teor de açúcar redutor em uma planta
CN113271768B (zh) 2018-12-30 2024-02-06 菲利普莫里斯生产公司 经由硝酸盐还原酶的突变来调节植物中的硝酸盐水平
US12419264B2 (en) * 2019-10-01 2025-09-23 Philip Morris Products S.A. Modulating sugar and amino acid content in a plant (SULTR3)
BR112022005552A2 (pt) 2019-10-01 2022-06-28 Philip Morris Products Sa Modulação da redução do teor de açúcar em uma planta (inv)
CN112931182B (zh) * 2021-01-29 2022-06-07 四川农业大学 一种提高小麦染色体重组频率的方法
WO2023036691A1 (en) 2021-09-10 2023-03-16 Philip Morris Products S.A. Modulating alkaloid profiles in nicotiana tabacum
WO2023117661A1 (en) 2021-12-20 2023-06-29 Philip Morris Products S.A. Increasing anatabine in tobacco leaf by regulating methyl putrescine oxidase
WO2023117701A1 (en) 2021-12-21 2023-06-29 Philip Morris Products S.A. Modulation of nicotine production by alteration of nicotinamidase expression or function in plants
CN114766359B (zh) * 2022-04-22 2023-03-31 华中农业大学 一种试管苗支架及其在柑橘茎尖微芽嫁接脱毒中的应用
JP2025533986A (ja) 2022-10-13 2025-10-09 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム Ntp2を調節することによって葉バイオマスおよび窒素利用効率を増加させる
CN120476136A (zh) 2023-02-02 2025-08-12 菲利普莫里斯生产公司 编码赖氨酸酮戊二酸还原酶的基因的调节
KR20250139371A (ko) 2023-02-02 2025-09-23 필립모리스 프로덕츠 에스.에이. 당 수송체의 조절
CN116574719B (zh) * 2023-06-28 2024-06-25 四川农业大学 AsMIPS1基因克隆、表达载体构建和应用
WO2025003105A1 (en) 2023-06-29 2025-01-02 Philip Morris Products S.A. Modulation of genes coding for glutamate dehydrogenase
CN119876252B (zh) * 2025-03-27 2025-06-20 浙江农林大学 毛竹PeNRAMP5基因在降低水稻Cd积累增加Mn吸收的应用

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WO2007035752A2 (en) * 2005-09-20 2007-03-29 J.R. Simplot Company Low acrylamide foods
RU2324737C1 (ru) * 2006-10-18 2008-05-20 Институт цитологии и генетики Сибирского отделения Российской академии наук (СО РАН) Способ получения трансгенных растений табака с повышенным содержанием пролина
US20130068240A1 (en) * 2011-09-21 2013-03-21 Patsy Elizabeth Elliott Tobacco Having Reduced Amounts of Amino Acids and Methods for Producing Such Lines

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JP4953808B2 (ja) 2006-12-29 2012-06-13 フルタ電機株式会社 葉たばこの監視制御盤の制御盤により制御する乾燥方法
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Publication number Priority date Publication date Assignee Title
WO2007035752A2 (en) * 2005-09-20 2007-03-29 J.R. Simplot Company Low acrylamide foods
RU2324737C1 (ru) * 2006-10-18 2008-05-20 Институт цитологии и генетики Сибирского отделения Российской академии наук (СО РАН) Способ получения трансгенных растений табака с повышенным содержанием пролина
US20130068240A1 (en) * 2011-09-21 2013-03-21 Patsy Elizabeth Elliott Tobacco Having Reduced Amounts of Amino Acids and Methods for Producing Such Lines

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Also Published As

Publication number Publication date
RU2018112407A3 (enExample) 2020-02-25
CN107920489A (zh) 2018-04-17
KR102207550B1 (ko) 2021-01-27
AU2016318423B2 (en) 2022-01-20
ES3014919T3 (en) 2025-04-28
NZ738897A (en) 2019-10-25
US10883114B2 (en) 2021-01-05
CA2998286A1 (en) 2017-03-16
BR112018002914A2 (pt) 2018-11-06
CA2998286C (en) 2023-11-07
EP3346826B8 (en) 2025-04-16
UA125478C2 (uk) 2022-03-30
PH12017502292A1 (en) 2018-06-25
JP6810134B2 (ja) 2021-01-06
HK1255341A1 (zh) 2019-08-16
US20190169628A1 (en) 2019-06-06
IL257305B (en) 2022-06-01
MX392152B (es) 2025-03-21
EP3346826C0 (en) 2025-02-19
JP2018527934A (ja) 2018-09-27
AR105968A1 (es) 2017-11-29
RU2018112407A (ru) 2019-10-09
PL3346826T3 (pl) 2025-06-09
KR20180051530A (ko) 2018-05-16
EP3346826B1 (en) 2025-02-19
AU2016318423A1 (en) 2018-04-05
EP3346826A1 (en) 2018-07-18
MX2018002695A (es) 2018-04-13
WO2017042162A1 (en) 2017-03-16
ZA201708411B (en) 2019-01-30
MY187046A (en) 2021-08-27
IL257305A (en) 2018-03-29

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