JP2018522543A5 - - Google Patents

Download PDF

Info

Publication number
JP2018522543A5
JP2018522543A5 JP2017564364A JP2017564364A JP2018522543A5 JP 2018522543 A5 JP2018522543 A5 JP 2018522543A5 JP 2017564364 A JP2017564364 A JP 2017564364A JP 2017564364 A JP2017564364 A JP 2017564364A JP 2018522543 A5 JP2018522543 A5 JP 2018522543A5
Authority
JP
Japan
Prior art keywords
sequence
polynucleotides
tag
tagged
oligonucleotide tags
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2017564364A
Other languages
English (en)
Japanese (ja)
Other versions
JP2018522543A (ja
JP6698708B2 (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2016/036763 external-priority patent/WO2016201142A1/en
Publication of JP2018522543A publication Critical patent/JP2018522543A/ja
Publication of JP2018522543A5 publication Critical patent/JP2018522543A5/ja
Application granted granted Critical
Publication of JP6698708B2 publication Critical patent/JP6698708B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2017564364A 2015-06-09 2016-06-09 分子タグ付けのための方法、システム、組成物、キット、装置、及びコンピュータ可読媒体 Active JP6698708B2 (ja)

Applications Claiming Priority (15)

Application Number Priority Date Filing Date Title
US201562172836P 2015-06-09 2015-06-09
US62/172,836 2015-06-09
US201562207177P 2015-08-19 2015-08-19
US62/207,177 2015-08-19
US201562248978P 2015-10-30 2015-10-30
US62/248,978 2015-10-30
US201662304530P 2016-03-07 2016-03-07
US62/304,530 2016-03-07
US201662310647P 2016-03-18 2016-03-18
US62/310,647 2016-03-18
US201662311276P 2016-03-21 2016-03-21
US62/311,276 2016-03-21
US201662323142P 2016-04-15 2016-04-15
US62/323,142 2016-04-15
PCT/US2016/036763 WO2016201142A1 (en) 2015-06-09 2016-06-09 Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging

Publications (3)

Publication Number Publication Date
JP2018522543A JP2018522543A (ja) 2018-08-16
JP2018522543A5 true JP2018522543A5 (enExample) 2019-07-04
JP6698708B2 JP6698708B2 (ja) 2020-05-27

Family

ID=56292898

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2017564364A Active JP6698708B2 (ja) 2015-06-09 2016-06-09 分子タグ付けのための方法、システム、組成物、キット、装置、及びコンピュータ可読媒体

Country Status (5)

Country Link
US (3) US10344336B2 (enExample)
EP (2) EP3307908B1 (enExample)
JP (1) JP6698708B2 (enExample)
CN (1) CN107849600A (enExample)
WO (1) WO2016201142A1 (enExample)

Families Citing this family (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015113725A1 (en) * 2014-02-03 2015-08-06 Thermo Fisher Scientific Baltics Uab Method for controlled dna fragmentation
US11136576B2 (en) 2014-02-03 2021-10-05 Thermo Fisher Scientific Baltics Uab Method for controlled DNA fragmentation
US10196678B2 (en) 2014-10-06 2019-02-05 ALVEO Technologies Inc. System and method for detection of nucleic acids
WO2016201142A1 (en) 2015-06-09 2016-12-15 Life Technologies Corporation Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging
WO2017100441A1 (en) 2015-12-08 2017-06-15 Twinstrand Biosciences, Inc. Improved adapters, methods, and compositions for duplex sequencing
AU2017330304A1 (en) 2016-09-23 2019-04-11 Alveo Technologies, Inc. Methods and compositions for detecting analytes
BR112019005810A2 (pt) 2016-09-27 2019-06-25 uBiome, Inc. método para sequenciamento de microbioma melhorado para caracterizar uma afecção relacionada a micro-organismo para um usuário associado a uma amostra biológica, e método para sequenciamento de microbioma melhorado
CN110312825A (zh) 2016-10-24 2019-10-08 吉内恩福赛克公司 隐藏核酸内存在的信息
WO2018089944A1 (en) 2016-11-11 2018-05-17 uBiome, Inc. Method and system for fragment assembly and sequence identification
CA3194557A1 (en) * 2017-01-20 2018-07-26 Sequenom, Inc. Sequencing adapter manufacture and use
CN110914911B (zh) 2017-05-16 2023-09-22 生命科技股份有限公司 压缩分子标记的核酸序列数据的方法
US12209274B2 (en) * 2017-05-31 2025-01-28 Centrillion Technology Holdings Corporation Oligonucleotide probe array with electronic detection system
US11542540B2 (en) 2017-06-16 2023-01-03 Life Technologies Corporation Control nucleic acids, and compositions, kits, and uses thereof
WO2018236631A1 (en) * 2017-06-20 2018-12-27 Illumina, Inc. METHODS AND COMPOSITIONS FOR RESOLVING INEFFICIENCY IN AMPLIFICATION REACTIONS
JP2020532976A (ja) * 2017-09-14 2020-11-19 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 環状一本鎖dnaライブラリーを生成するための新規な方法
CN111108218B (zh) 2017-09-20 2024-06-25 生命科技股份有限公司 使用压缩的分子标记的核酸序列数据检测融合的方法
US20190241982A1 (en) * 2018-02-02 2019-08-08 Apdn (B.V.I.) Inc. Systems and methods for tracking the origin of cannabis products and cannabis derivative products
GB201804641D0 (en) * 2018-03-22 2018-05-09 Inivata Ltd Methods of sequencing nucleic acids and error correction of sequence reads
GB201804642D0 (en) * 2018-03-22 2018-05-09 Inivata Ltd Methods of labelling nucleic acids
CA3302676A1 (en) 2018-04-02 2026-05-13 Grail, Inc. Methylation markers and targeted methylation probe panels
CN108486230B (zh) * 2018-05-18 2022-02-08 中国人民解放军陆军军医大学第一附属医院 用于无创检测mitf基因突变的试剂盒及其制备方法
AU2019287163B2 (en) 2018-06-12 2025-08-21 Keygene N.V. Nucleic acid amplification method
CN108959851B (zh) * 2018-06-12 2022-03-18 哈尔滨工程大学 一种Illumina高通量测序数据误差校正方法
JP7407193B2 (ja) 2018-08-08 2023-12-28 イニヴァータ リミテッド 可変の複製多重pcrを使用した配列決定方法
WO2020036926A1 (en) * 2018-08-17 2020-02-20 Cellecta, Inc. Multiplex preparation of barcoded gene specific dna fragments
MY204234A (en) 2018-08-27 2024-08-16 Regeneron Pharma Use of raman spectroscopy in downstream purification
EP3856903A4 (en) 2018-09-27 2022-07-27 Grail, LLC METHYLATION MARKER AND TARGETED METHYLATION PROBE PANEL
DE202019005610U1 (de) * 2018-12-07 2021-06-10 Element Biosciences, Inc. Durchflusszellenvorrichtung und ihre Verwendung
BR112020026667A2 (pt) * 2018-12-19 2021-07-27 Illumina, Inc. métodos para melhorar a prioridade de clonalidade do agrupamento de polinucleotídeo
AU2019401601B2 (en) 2018-12-20 2026-04-02 Alveo Technologies, Inc. Handheld impedance-based diagnostic test system for detecting analytes
AU2019402900A1 (en) * 2018-12-20 2021-07-15 Alveo Technologies, Inc. Isothermal amplification with electrical detection
US12139753B2 (en) 2019-03-22 2024-11-12 Life Technologies Corporation Methods for partner agnostic gene fusion detection
EP3748015A1 (en) * 2019-06-04 2020-12-09 Sysmex Corporation Method for analyzing nucleic acid sequence, apparatus for analyzing nucleic acid sequence, and program for analyzing nucleic acid sequence
EP3748014A1 (en) 2019-06-04 2020-12-09 Sysmex Corporation Method for analyzing nucleic acid sequence, apparatus for analyzing nucleic acid sequence, and program for analyzing nucleic acid sequence
EP3748013A1 (en) * 2019-06-04 2020-12-09 Sysmex Corporation Method for analyzing a nucleic acid sequence
CN114514328A (zh) * 2019-08-21 2022-05-17 生命技术公司 用于测序的系统和方法
WO2021042131A1 (en) * 2019-08-30 2021-03-04 Life Technologies Corporation Compositions and methods for oncology precision assays
WO2021137845A1 (en) * 2019-12-30 2021-07-08 Dna Syntech Inc. Method for preparing nucleic acid sequences using enzyme
WO2021146187A1 (en) 2020-01-13 2021-07-22 Fluent Biosciences Inc. Emulsion based drug screening
JP2023511279A (ja) 2020-01-13 2023-03-17 フルーエント バイオサイエンシーズ インコーポレイテッド 単一細胞シーケンシング
JP7730449B2 (ja) 2020-01-13 2025-08-28 イルミナ インコーポレイテッド 単一細胞遺伝子プロファイリングのための方法およびシステム
US12663345B2 (en) 2020-01-13 2026-06-23 Illumina, Inc. Devices for generating monodisperse droplets from a bulk liquid
WO2021146168A1 (en) * 2020-01-13 2021-07-22 Fluent Biosciences Inc. Methods and systems for amplifying low concentrations of nucleic acids
CN111254198A (zh) * 2020-03-09 2020-06-09 苏州宏元生物科技有限公司 一种尿路上皮癌诊断和预后评估试剂盒
CA3175931A1 (en) 2020-03-16 2021-09-23 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
US12428685B2 (en) 2020-03-24 2025-09-30 Illumina, Inc. Viral detection using template emulsification
CN111466508A (zh) * 2020-04-10 2020-07-31 四川长虹电器股份有限公司 射频解冻装置、冰箱及射频解冻方法
JP7828901B2 (ja) 2020-06-18 2026-03-12 リゾルブ バイオサイエンシズ ゲゼルシャフト ミット ベシュレンクテル ハフツング 試料中の異なる分析物及び分析物の異なるサブグループ/変形物を検出するための多重的方法
CA3189597A1 (en) 2020-07-15 2022-01-20 Fluent Biosciences Inc. Tiered ligation oligos
US12472492B2 (en) 2020-08-14 2025-11-18 Alveo Technologies, Inc. Systems and methods of sample depositing and testing
CN117083394A (zh) 2020-11-14 2023-11-17 生命技术公司 用于自动重复测序的系统和方法
WO2022251445A1 (en) 2021-05-28 2022-12-01 Fluent Biosciences Inc. Spatial transcriptomics in pips
US11220707B1 (en) 2021-06-17 2022-01-11 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
US11859241B2 (en) 2021-06-17 2024-01-02 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
CN119061125A (zh) 2021-07-21 2024-12-03 元素生物科学公司 用于核酸测序的方法
EP4426475A4 (en) 2021-11-03 2025-12-24 Fluent Biosciences Inc DEVICES TO IMPROVE THE PREPARATION AND PROCESSING OF SAMPLES
CA3264896A1 (en) 2022-08-15 2024-02-22 Element Biosciences, Inc. SPATIAL RESOLUTION SURFACE CAPTURE OF NUCLEIC ACIDS
US12534721B2 (en) 2022-10-06 2026-01-27 Illumina, Inc. Quantitative detection and analysis of moleculesip
EP4627115A1 (en) 2022-11-29 2025-10-08 Element Biosciences, Inc. Flow cell devices and optical systems for nucleic acid sequencing
US12605715B2 (en) * 2022-12-02 2026-04-21 Celo Biotech Inc. Magnetic stand
US20240294982A1 (en) * 2023-03-01 2024-09-05 Personal Genomics Taiwan, Inc. Method, kit and cartridge for detecting nucleic acid molecule
EP4680764A2 (en) * 2023-03-17 2026-01-21 Bruker Spatial Biology, Inc. Assay for recombinase accessible chromatin and related compositions and methods
WO2025085618A1 (en) * 2023-10-20 2025-04-24 Illumina, Inc. Methods for appending adaptors onto polynucleotides
WO2026107240A1 (en) * 2024-11-15 2026-05-21 Neogenomics Laboratories, Inc. Methods and systems for analyzing low-input samples

Family Cites Families (136)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5333675C1 (en) 1986-02-25 2001-05-01 Perkin Elmer Corp Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
CA2036946C (en) 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US5273881A (en) 1990-05-07 1993-12-28 Daikin Industries, Ltd. Diagnostic applications of double D-loop formation
KR100245284B1 (ko) 1990-05-07 2000-03-02 이노우에 노리유끼 이중 d-루프 형성의 진단학적 응용
US5194370A (en) 1990-05-16 1993-03-16 Life Technologies, Inc. Promoter ligation activated transcription amplification of nucleic acid sequences
US6261808B1 (en) 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
US5695934A (en) 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5882856A (en) 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification
US6444806B1 (en) 1996-04-30 2002-09-03 Hisamitsu Pharmaceutical Co., Inc. Conjugates and methods of forming conjugates of oligonucleotides and carbohydrates
EP2327797B1 (en) 1997-04-01 2015-11-25 Illumina Cambridge Limited Method of nucleic acid sequencing
ATE358177T1 (de) 1997-07-07 2007-04-15 Medical Res Council Ein in vitro sortierverfahren
FI103809B1 (fi) 1997-07-14 1999-09-30 Finnzymes Oy In vitro -menetelmä templaattien tuottamiseksi DNA-sekventointia varten
US6607878B2 (en) 1997-10-06 2003-08-19 Stratagene Collections of uniquely tagged molecules
WO2000015779A2 (en) 1998-09-15 2000-03-23 Yale University Molecular cloning using rolling circle amplification
US6287825B1 (en) 1998-09-18 2001-09-11 Molecular Staging Inc. Methods for reducing the complexity of DNA sequences
US6480791B1 (en) 1998-10-28 2002-11-12 Michael P. Strathmann Parallel methods for genomic analysis
US6830884B1 (en) 1998-12-15 2004-12-14 Molecular Staging Inc. Method of amplification
US6521428B1 (en) 1999-04-21 2003-02-18 Genome Technologies, Llc Shot-gun sequencing and amplification without cloning
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
JP2003521252A (ja) 2000-02-07 2003-07-15 イルミナ インコーポレイテッド ユニバーサルプライミングを用いる核酸検出方法
EP3034627B1 (en) 2000-10-06 2019-01-30 The Trustees of Columbia University in the City of New York Massive parallel method for decoding dna and rna
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
GB2378245A (en) 2001-08-03 2003-02-05 Mats Nilsson Nucleic acid amplification method
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
WO2003050304A1 (en) 2001-12-08 2003-06-19 Seegene, Inc Annealing control primer system for regulating primer annealing specificity and its applications
US20030175749A1 (en) 2001-12-08 2003-09-18 Jong-Yoon Chun Annealing control primer and its uses
EP1319718A1 (en) 2001-12-14 2003-06-18 Keygene N.V. High throughput analysis and detection of multiple target sequences
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
DK1499738T3 (da) 2002-02-21 2008-11-17 Asm Scient Inc Recombinase-polymerease-amplifikation
GB0208768D0 (en) 2002-04-17 2002-05-29 Univ Newcastle DNA polymerases
AU2003259350A1 (en) 2002-08-23 2004-03-11 Solexa Limited Modified nucleotides for polynucleotide sequencing
US20040038331A1 (en) 2002-08-23 2004-02-26 Reddy M. Parameswara Solid phase synthesis of biomolecule conjugates
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
DE602004026033D1 (de) 2003-01-29 2010-04-29 454 Corp Zweiendige sequenzierung
US20080261204A1 (en) 2004-01-23 2008-10-23 Lingvitae As Polynucleotide Ligation Reactions
EP2857523A1 (en) 2005-02-01 2015-04-08 Applied Biosystems, LLC Method for identifying a sequence in a polynucleotide
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
DE602006015633D1 (de) 2005-04-29 2010-09-02 Synthetic Genomics Inc Amplifikation und klonierung einzelner dna-moleküle mittels rolling-circle-amplifikation
US20060263789A1 (en) 2005-05-19 2006-11-23 Robert Kincaid Unique identifiers for indicating properties associated with entities to which they are attached, and methods for using
US8785353B2 (en) 2005-06-23 2014-07-22 Keygene N.V. Strategies for high throughput identification and detection of polymorphisms
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
EP2829615B1 (en) 2005-07-25 2018-05-09 Alere San Diego, Inc. Kit for multiplexing recombinase polymerase amplification
DK1924704T3 (da) 2005-08-02 2011-09-05 Rubicon Genomics Inc Sammensætninger og fremgangsmåder til bearbejdning og mangfoldiggørelse af DNA, herunder ved anvendelse af flere enzymer i en enkelt reaktion
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
CA2910861C (en) 2005-09-29 2018-08-07 Michael Josephus Theresia Van Eijk High throughput screening of mutagenized populations
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
WO2007111937A1 (en) 2006-03-23 2007-10-04 Applera Corporation Directed enrichment of genomic dna for high-throughput sequencing
EP2029782B1 (en) 2006-05-04 2014-11-26 Alere San Diego, Inc. Recombinase polymerase amplification
US8644588B2 (en) 2006-09-20 2014-02-04 Luminescent Technologies, Inc. Photo-mask and wafer image reconstruction
EP2071927A2 (en) 2006-09-28 2009-06-24 Illumina, Inc. Compositions and methods for nucleotide sequencing
GB2457402B (en) 2006-12-01 2011-10-19 Univ Columbia Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
EP2677309B9 (en) 2006-12-14 2014-11-19 Life Technologies Corporation Methods for sequencing a nucleic acid using large scale FET arrays, configured to measure a limited pH range
US7932034B2 (en) 2006-12-20 2011-04-26 The Board Of Trustees Of The Leland Stanford Junior University Heat and pH measurement for sequencing of DNA
EP2121983A2 (en) 2007-02-02 2009-11-25 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
CA2679586A1 (en) 2007-02-27 2008-10-23 Northwestern University Molecule attachment to nanoparticles
EP2203566A4 (en) 2007-09-28 2011-02-09 Pacific Biosciences California ERROR-FREE DNA AMPLIFICATION FOR CLONAL SEQUENCING
JP2011515102A (ja) 2008-03-28 2011-05-19 パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド 核酸シーケンシング用組成物及び方法
US8271206B2 (en) * 2008-04-21 2012-09-18 Softgenetics Llc DNA sequence assembly methods of short reads
WO2009133466A2 (en) 2008-04-30 2009-11-05 Population Genetics Technologies Ltd. Asymmetric adapter library construction
GB2461127B (en) 2008-06-25 2010-07-14 Ion Torrent Systems Inc Methods and apparatus for measuring analytes using large scale FET arrays
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US8546128B2 (en) 2008-10-22 2013-10-01 Life Technologies Corporation Fluidics system for sequential delivery of reagents
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US9394567B2 (en) * 2008-11-07 2016-07-19 Adaptive Biotechnologies Corporation Detection and quantification of sample contamination in immune repertoire analysis
EP2358875B1 (en) 2008-11-19 2015-08-12 Amyris, Inc. Compositions and methods for the assembly of polynucleotides
US9085798B2 (en) 2009-04-30 2015-07-21 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
US20120156728A1 (en) 2010-12-17 2012-06-21 Life Technologies Corporation Clonal amplification of nucleic acid on solid surface with template walking
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US9309566B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
US9334531B2 (en) 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
WO2010141940A1 (en) 2009-06-05 2010-12-09 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
WO2012024500A1 (en) 2010-08-18 2012-02-23 Life Technologies Corporation Chemical coating of microwell for electrochemical detection device
EP2619327B1 (en) 2010-09-21 2014-10-22 Population Genetics Technologies LTD. Increasing confidence of allele calls with molecular counting
EP2633470B1 (en) 2010-10-27 2016-10-26 Life Technologies Corporation Predictive model for use in sequencing-by-synthesis
US20130060482A1 (en) 2010-12-30 2013-03-07 Life Technologies Corporation Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
EP3225697A3 (en) 2010-12-30 2017-11-22 Foundation Medicine, Inc. Optimization of multigene analysis of tumor samples
US20130090860A1 (en) 2010-12-30 2013-04-11 Life Technologies Corporation Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
EP2668273B9 (en) 2011-01-28 2017-03-29 Illumina, Inc. Oligonucleotide replacement for di-tagged and directional libraries
US9260753B2 (en) 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
EP3978620A1 (en) 2011-04-07 2022-04-06 Abbott Diagnostics Scarborough, Inc. Monitoring recombinase polymerase amplification mixtures
US9617296B2 (en) 2011-04-08 2017-04-11 Life Technologies Corporation Methods and kits for breaking emulsions
EP3246416B1 (en) 2011-04-15 2024-06-05 The Johns Hopkins University Safe sequencing system
US9809904B2 (en) * 2011-04-21 2017-11-07 University Of Washington Through Its Center For Commercialization Methods for retrieval of sequence-verified DNA constructs
EP2714928B1 (en) 2011-05-27 2017-08-02 Life Technologies Corporation Methods for manipulating biomolecules
US20130017978A1 (en) 2011-07-11 2013-01-17 Finnzymes Oy Methods and transposon nucleic acids for generating a dna library
US9145623B2 (en) 2011-07-20 2015-09-29 Thermo Fisher Scientific Oy Transposon nucleic acids comprising a calibration sequence for DNA sequencing
WO2013130512A2 (en) * 2012-02-27 2013-09-06 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
EP3321378B1 (en) * 2012-02-27 2021-11-10 Becton, Dickinson and Company Compositions for molecular counting
US9862995B2 (en) * 2012-03-13 2018-01-09 Abhijit Ajit Patel Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
WO2013142389A1 (en) 2012-03-20 2013-09-26 University Of Washington Through Its Center For Commercialization Methods of lowering the error rate of massively parallel dna sequencing using duplex consensus sequencing
AU2013267609C1 (en) 2012-05-31 2019-01-03 Board Of Regents, The University Of Texas System Method for accurate sequencing of DNA
WO2013188840A1 (en) 2012-06-14 2013-12-19 Fred Hutchinson Cancer Research Center Compositions and methods for sensitive mutation detection in nucleic acid molecules
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
WO2014026031A1 (en) 2012-08-10 2014-02-13 Sequenta, Inc. High sensitivity mutation detection using sequence tags
US20140066317A1 (en) * 2012-09-04 2014-03-06 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
PL2893040T3 (pl) 2012-09-04 2019-05-31 Guardant Health Inc Sposoby wykrywania rzadkich mutacji i wariantu liczby kopii
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
CN103045726B (zh) * 2012-11-20 2015-11-25 南方科技大学 对多个混合dna或rna序列进行基因测序的方法及装置
CN105531375B (zh) 2012-12-10 2020-03-03 分析生物科学有限公司 靶向基因组分析的方法
HK1217971A1 (zh) * 2013-02-21 2017-01-27 Toma Biosciences Inc 用於核酸分析的方法、組合物和試劑盒
US20140255929A1 (en) * 2013-03-11 2014-09-11 Sequenta, Inc. Mosaic tags for labeling templates in large-scale amplifications
WO2014151961A1 (en) 2013-03-14 2014-09-25 Life Technologies Corporation Matrix arrays and methods for making same
ITMI20130496A1 (it) 2013-03-29 2014-09-30 Atlas Copco Blm Srl Gruppo e disposizione di montaggio di una spina di un connettore circolare a baionetta su un pannello o una parete, in particolare un pannello o una parete di un corpo scatolare di contenimento di un dispositivo elettrico o elettronico
US20140357499A1 (en) * 2013-05-30 2014-12-04 Washington University METHODS OF LOW ERROR AMPLICON SEQUENCING (LEA-Seq) AND THE USE THEREOF
WO2015031559A1 (en) 2013-08-30 2015-03-05 Tensor Surgical, Inc Passive retrieving interosseous suture passing device
GB2518668B (en) 2013-09-28 2017-07-12 Design Blue Ltd Flexible pads and shield systems
US9476853B2 (en) 2013-12-10 2016-10-25 Life Technologies Corporation System and method for forming microwells
CN111534580B (zh) 2013-12-28 2024-06-04 夸登特健康公司 用于检测遗传变异的方法和系统
JP6484636B2 (ja) 2014-01-16 2019-03-13 イラミーナ インコーポレーテッド 固相担体におけるアンプリコン調製および配列決定
WO2015113725A1 (en) 2014-02-03 2015-08-06 Thermo Fisher Scientific Baltics Uab Method for controlled dna fragmentation
WO2016004244A1 (en) 2014-07-02 2016-01-07 Life Technologies Corporation Surface treatment of semiconductor sensors
EP3169799B1 (en) 2014-07-15 2019-09-04 Qiagen Sciences, LLC Semi-random barcodes for nucleic acid analysis
WO2016201142A1 (en) 2015-06-09 2016-12-15 Life Technologies Corporation Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging

Similar Documents

Publication Publication Date Title
US20230295704A1 (en) Barcoded Protein Array for Multiplex Single-Molecule Interaction Profiling
US7378242B2 (en) DNA sequence detection by limited primer extension
US12516446B2 (en) Array and method for detecting spatial information of nucleic acids
US11326206B2 (en) Methods of quantifying target nucleic acids and identifying sequence variants
US20140364323A1 (en) Multi-sample indexing for multiplex genotyping
CA2505254A1 (en) Universal tag assay
KR20210142690A (ko) 시스템
CN110878345A (zh) 通过分子计数提高等位基因调用的置信度
JP2023539360A5 (enExample)
JP2013507992A5 (enExample)
US8685646B2 (en) Methods and reagents for shortening incubation times in hybridization assays
US20240167088A1 (en) Methods and devices of generating clusters of amplicons
US20220064726A1 (en) Method for detecting polymerase incorporation of nucleotides
JP2016527904A5 (enExample)
MX2010012275A (es) Metodo para detectar agentes virales respiratorios en una muestra de ensayo.
WO2008119046A3 (en) Methods and compositions for multiplexing detection of nucleic acid sequences within an array element
US7501254B2 (en) Methods and compositions for amplification and capture of nucleic acid sequences
US11739373B2 (en) Systems and methods for multiplexed analyte detection using antibody-oligonucleotide conjugates
CN117625768A (zh) 一种通用数字pcr检测体系及其应用
US7914983B2 (en) Detection method for gene expression
US10876153B2 (en) Nucleic acid detection method
JP4535266B2 (ja) Dnaコンピュータ技術による核酸の定量検出方法
CN115976179B (zh) 一种高灵敏度探针及其制备方法和应用
WO2020132714A1 (en) Method for amplification of nucleic acids
US9145586B2 (en) Method of labeling a target nucleic acid