JP2018513685A5 - - Google Patents
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- JP2018513685A5 JP2018513685A5 JP2017553115A JP2017553115A JP2018513685A5 JP 2018513685 A5 JP2018513685 A5 JP 2018513685A5 JP 2017553115 A JP2017553115 A JP 2017553115A JP 2017553115 A JP2017553115 A JP 2017553115A JP 2018513685 A5 JP2018513685 A5 JP 2018513685A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- complementary region
- region
- mir
- strand
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108091070501 miRNA Proteins 0.000 claims description 17
- 239000002679 microRNA Substances 0.000 claims description 17
- 230000000295 complement effect Effects 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 13
- 108020004707 nucleic acids Proteins 0.000 claims description 12
- 150000007523 nucleic acids Chemical class 0.000 claims description 12
- 102000039446 nucleic acids Human genes 0.000 claims description 12
- 230000000903 blocking effect Effects 0.000 claims 14
- 108090000364 Ligases Proteins 0.000 claims 4
- 102000003960 Ligases Human genes 0.000 claims 4
- 239000000758 substrate Substances 0.000 claims 4
- 238000000137 annealing Methods 0.000 claims 3
- -1 mir-15a Proteins 0.000 claims 3
- 238000003757 reverse transcription PCR Methods 0.000 claims 3
- 108010061982 DNA Ligases Proteins 0.000 claims 1
- 101710086015 RNA ligase Proteins 0.000 claims 1
- 230000006154 adenylylation Effects 0.000 claims 1
- 108091031326 miR-15b stem-loop Proteins 0.000 claims 1
- 108091027943 miR-16 stem-loop Proteins 0.000 claims 1
- 108091046553 miR-26 stem-loop Proteins 0.000 claims 1
- 108091023821 miR-26-1 stem-loop Proteins 0.000 claims 1
- 108091045094 miR-26-2 stem-loop Proteins 0.000 claims 1
- 108091030938 miR-424 stem-loop Proteins 0.000 claims 1
- 108091052738 miR-486-1 stem-loop Proteins 0.000 claims 1
- 108091030654 miR-486-2 stem-loop Proteins 0.000 claims 1
- 108091063340 miR-497 stem-loop Proteins 0.000 claims 1
- 238000009396 hybridization Methods 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562146015P | 2015-04-10 | 2015-04-10 | |
| US62/146,015 | 2015-04-10 | ||
| PCT/US2016/026846 WO2016164866A1 (en) | 2015-04-10 | 2016-04-10 | METHOD FOR BLOCKING miRNA |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018513685A JP2018513685A (ja) | 2018-05-31 |
| JP2018513685A5 true JP2018513685A5 (enExample) | 2020-07-02 |
| JP6791875B2 JP6791875B2 (ja) | 2020-11-25 |
Family
ID=57072747
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017553115A Active JP6791875B2 (ja) | 2015-04-10 | 2016-04-10 | ｍｉＲＮＡをブロックするための方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US10480023B2 (enExample) |
| EP (1) | EP3280805B1 (enExample) |
| JP (1) | JP6791875B2 (enExample) |
| AU (1) | AU2016246820B2 (enExample) |
| CA (1) | CA2982369A1 (enExample) |
| IL (1) | IL254972B (enExample) |
| MX (1) | MX386064B (enExample) |
| WO (1) | WO2016164866A1 (enExample) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016161388A1 (en) | 2015-04-03 | 2016-10-06 | University Of Massachusetts | Fully stabilized asymmetric sirna |
| WO2016196824A1 (en) | 2015-06-02 | 2016-12-08 | Children's Medical Center Corporation | Nucleic acid complexes for screening barcoded compounds |
| US12077807B2 (en) | 2015-06-27 | 2024-09-03 | The Research Foundation For The State University Of New York | Compositions and methods for analyte detection using nanoswitches |
| WO2017030973A1 (en) | 2015-08-14 | 2017-02-23 | University Of Massachusetts | Bioactive conjugates for oligonucleotide delivery |
| JP7749201B6 (ja) | 2016-01-31 | 2025-10-21 | ユニバーシティー オブ マサチューセッツ | 分岐オリゴヌクレオチド |
| US10287625B2 (en) * | 2016-03-18 | 2019-05-14 | Norgen Biotek Corp. | Methods and kits for separating nucleic acids by size |
| WO2017165647A1 (en) | 2016-03-23 | 2017-09-28 | Children's Medical Center Corporation | Rapid and sensitive detection and quantification of analytes in complex samples using polymer-based methods |
| CA3033368A1 (en) | 2016-08-12 | 2018-02-15 | University Of Massachusetts | Conjugated oligonucleotides |
| WO2018106721A1 (en) * | 2016-12-05 | 2018-06-14 | Children's Medical Center Corporation | Nucleic acid nanoswitch construction methods |
| WO2018157100A1 (en) * | 2017-02-27 | 2018-08-30 | Bioo Scientific Corporation | Methods and kits for depleting undesired nucleic acids |
| CN110799647A (zh) | 2017-06-23 | 2020-02-14 | 马萨诸塞大学 | 双尾自递送sirna及相关的方法 |
| CN111278844B (zh) * | 2017-09-13 | 2023-12-19 | 贝克顿·迪金森公司 | 用于使用氧化铁颗粒提取核酸的方法和组合物 |
| US11591636B2 (en) | 2017-11-20 | 2023-02-28 | Children's Medical Center Corporation | Force-controlled nanoswitch assays for single-molecule detection in complex biological fluids |
| CN108676846B (zh) * | 2018-05-25 | 2022-04-01 | 艾吉泰康生物科技(北京)有限公司 | 一种桥式寡核苷酸在文库目标区域捕获中的应用 |
| EP3833763A4 (en) | 2018-08-10 | 2023-07-19 | University of Massachusetts | MODIFIED OLIGONUCLEOTIDES FOR TARGETING SNPS |
| US11279930B2 (en) | 2018-08-23 | 2022-03-22 | University Of Massachusetts | O-methyl rich fully stabilized oligonucleotides |
| JP2022523467A (ja) | 2019-01-18 | 2022-04-25 | ユニバーシティ・オブ・マサチューセッツ | 動的な薬物動態を修飾するアンカー |
| CN114502730A (zh) | 2019-08-09 | 2022-05-13 | 马萨诸塞大学 | 经化学修饰的靶向snp的寡核苷酸 |
| US12365894B2 (en) | 2019-09-16 | 2025-07-22 | University Of Massachusetts | Branched lipid conjugates of siRNA for specific tissue delivery |
| EP4359539A4 (en) | 2021-06-23 | 2025-05-14 | University Of Massachusetts | ANTI-FLT1 OLIGONUCLEOTIDE COMPOUNDS OPTIMIZED FOR THE TREATMENT OF PREECLAMPSIA AND OTHER ANGIOGENIC DISORDERS |
| WO2023066534A1 (en) * | 2021-10-22 | 2023-04-27 | Hummingbird Diagnostics Gmbh | Targeted inhibition of reverse transcription using antisense oligos |
| US20230323341A1 (en) * | 2022-03-25 | 2023-10-12 | Bioo Scientific Corporation | Methods, compositions, and kits for inhibiting formation of adapter dimers |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1253205A1 (en) | 2001-04-24 | 2002-10-30 | LION Bioscience AG | Method of blocking amplification of selected sequences |
| US20030211483A1 (en) * | 2002-05-09 | 2003-11-13 | Schroeder Benjamin G. | Methods for the enrichment of low-abundance polynucleotides |
| WO2007025281A2 (en) * | 2005-08-24 | 2007-03-01 | Applera Corporation | A method to quantify sirnas, mirnas and polymorphic mirnas |
| WO2010126913A1 (en) | 2009-04-27 | 2010-11-04 | Gen-Probe Incorporated | Methods and kits for use in the selective amplification of target sequences |
| CN102791877A (zh) * | 2009-11-06 | 2012-11-21 | 酶学有限公司 | 使用双链核酸复合物与耐热聚合酶用于合成脱氧核糖核苷酸链的组合物和方法 |
| CN103237901B (zh) * | 2010-03-01 | 2016-08-03 | 卡里斯生命科学瑞士控股有限责任公司 | 用于治疗诊断的生物标志物 |
| WO2012005572A1 (en) * | 2010-07-06 | 2012-01-12 | Interna Technologies Bv | Mirna and its diagnostic and therapeutic uses in diseases or conditions associated with melanoma, or in diseases or conditions associated with activated braf pathway |
| EP3620533B1 (en) * | 2011-09-06 | 2023-01-18 | Gen-Probe Incorporated | Closed nucleic acid structures |
| WO2014071322A1 (en) * | 2012-11-02 | 2014-05-08 | Life Technologies Corporation | Small RNA Capture, Detection and Quantification |
-
2016
- 2016-04-10 US US15/565,652 patent/US10480023B2/en active Active
- 2016-04-10 WO PCT/US2016/026846 patent/WO2016164866A1/en not_active Ceased
- 2016-04-10 MX MX2017013022A patent/MX386064B/es unknown
- 2016-04-10 AU AU2016246820A patent/AU2016246820B2/en active Active
- 2016-04-10 JP JP2017553115A patent/JP6791875B2/ja active Active
- 2016-04-10 EP EP16777458.7A patent/EP3280805B1/en active Active
- 2016-04-10 CA CA2982369A patent/CA2982369A1/en active Pending
-
2017
- 2017-10-10 IL IL254972A patent/IL254972B/en active IP Right Grant
-
2019
- 2019-09-24 US US16/581,129 patent/US11781176B2/en active Active
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