JP2018512891A5 - - Google Patents
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- JP2018512891A5 JP2018512891A5 JP2018503698A JP2018503698A JP2018512891A5 JP 2018512891 A5 JP2018512891 A5 JP 2018512891A5 JP 2018503698 A JP2018503698 A JP 2018503698A JP 2018503698 A JP2018503698 A JP 2018503698A JP 2018512891 A5 JP2018512891 A5 JP 2018512891A5
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- Prior art keywords
- polypeptide
- cell
- composition
- based shuttle
- shuttle agent
- Prior art date
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- 108090000765 processed proteins & peptides Proteins 0.000 claims 39
- 102000004196 processed proteins & peptides Human genes 0.000 claims 33
- 229920001184 polypeptide Polymers 0.000 claims 31
- 239000000203 mixture Substances 0.000 claims 25
- 239000003795 chemical substances by application Substances 0.000 claims 18
- 210000003527 eukaryotic cell Anatomy 0.000 claims 16
- 230000026683 transduction Effects 0.000 claims 10
- 238000010361 transduction Methods 0.000 claims 10
- 108091033409 CRISPR Proteins 0.000 claims 9
- 210000004027 cell Anatomy 0.000 claims 9
- 210000000172 cytosol Anatomy 0.000 claims 9
- -1 PTD4 Proteins 0.000 claims 8
- 108010076504 Protein Sorting Signals Proteins 0.000 claims 6
- 125000003275 alpha amino acid group Chemical group 0.000 claims 6
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims 6
- 230000035699 permeability Effects 0.000 claims 5
- 125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 claims 4
- 238000000034 method Methods 0.000 claims 4
- 108010077850 Nuclear Localization Signals Proteins 0.000 claims 3
- 230000000694 effects Effects 0.000 claims 3
- 230000002101 lytic effect Effects 0.000 claims 3
- 241000670727 Amida Species 0.000 claims 2
- 108010051109 Cell-Penetrating Peptides Proteins 0.000 claims 2
- 102000020313 Cell-Penetrating Peptides Human genes 0.000 claims 2
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- 101710135898 Myc proto-oncogene protein Proteins 0.000 claims 2
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- 241000737052 Naso hexacanthus Species 0.000 claims 2
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- 108010089610 Nuclear Proteins Proteins 0.000 claims 2
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- VDXZNPDIRNWWCW-JFTDCZMZSA-N melittin Chemical compound NCC(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(N)=O)CC1=CNC2=CC=CC=C12 VDXZNPDIRNWWCW-JFTDCZMZSA-N 0.000 claims 2
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- MCYTYTUNNNZWOK-LCLOTLQISA-N penetratin Chemical compound C([C@H](NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](N)CCCNC(N)=N)[C@@H](C)CC)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(N)=O)C1=CC=CC=C1 MCYTYTUNNNZWOK-LCLOTLQISA-N 0.000 claims 2
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- IEXIHWCBQMQDHC-KEIUHOJNSA-N 5,9-hexacosadienoic acid Chemical compound CCCCCCCCCCCCCCCC\C=C\CC\C=C\CCCC(O)=O IEXIHWCBQMQDHC-KEIUHOJNSA-N 0.000 claims 1
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- 108700031308 Antennapedia Homeodomain Proteins 0.000 claims 1
- 239000004475 Arginine Substances 0.000 claims 1
- 102100021663 Baculoviral IAP repeat-containing protein 5 Human genes 0.000 claims 1
- 238000010354 CRISPR gene editing Methods 0.000 claims 1
- 101100189913 Caenorhabditis elegans pept-1 gene Proteins 0.000 claims 1
- 108010078791 Carrier Proteins Proteins 0.000 claims 1
- 101800003223 Cecropin-A Proteins 0.000 claims 1
- 102000000634 Cytochrome c oxidase subunit IV Human genes 0.000 claims 1
- 108050008072 Cytochrome c oxidase subunit IV Proteins 0.000 claims 1
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- 108020004414 DNA Proteins 0.000 claims 1
- 101100129232 Danio rerio mafaa gene Proteins 0.000 claims 1
- 102000016607 Diphtheria Toxin Human genes 0.000 claims 1
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- 101100206886 Drosophila melanogaster Tim9a gene Proteins 0.000 claims 1
- 101150099612 Esrrb gene Proteins 0.000 claims 1
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- 102100028404 Homeobox protein Hox-B4 Human genes 0.000 claims 1
- 101000580577 Homo sapiens ATP-dependent DNA helicase Q4 Proteins 0.000 claims 1
- 101000877681 Homo sapiens Forkhead box protein O3 Proteins 0.000 claims 1
- 101000839788 Homo sapiens Homeobox protein Hox-B4 Proteins 0.000 claims 1
- 101001053263 Homo sapiens Insulin gene enhancer protein ISL-1 Proteins 0.000 claims 1
- 101000740178 Homo sapiens Sal-like protein 4 Proteins 0.000 claims 1
- 101000713602 Homo sapiens T-box transcription factor TBX21 Proteins 0.000 claims 1
- 101000711846 Homo sapiens Transcription factor SOX-9 Proteins 0.000 claims 1
- 102100022875 Hypoxia-inducible factor 1-alpha Human genes 0.000 claims 1
- 108050009527 Hypoxia-inducible factor-1 alpha Proteins 0.000 claims 1
- 102100024392 Insulin gene enhancer protein ISL-1 Human genes 0.000 claims 1
- 101150051019 Klrg1 gene Proteins 0.000 claims 1
- 108700021430 Kruppel-Like Factor 4 Proteins 0.000 claims 1
- 102100022699 Lymphoid enhancer-binding factor 1 Human genes 0.000 claims 1
- 108090001093 Lymphoid enhancer-binding factor 1 Proteins 0.000 claims 1
- 101150084866 MAFA gene Proteins 0.000 claims 1
- 101100381525 Mus musculus Bcl6 gene Proteins 0.000 claims 1
- 101100445364 Mus musculus Eomes gene Proteins 0.000 claims 1
- 101000687343 Mus musculus PR domain zinc finger protein 1 Proteins 0.000 claims 1
- 101100189471 Mus musculus Pbx1 gene Proteins 0.000 claims 1
- 101100078259 Mus musculus Runx1t1 gene Proteins 0.000 claims 1
- 102000002488 Nucleoplasmin Human genes 0.000 claims 1
- 108010062618 Oncogene Proteins v-rel Proteins 0.000 claims 1
- 102100041030 Pancreas/duodenum homeobox protein 1 Human genes 0.000 claims 1
- 101710144033 Pancreas/duodenum homeobox protein 1 Proteins 0.000 claims 1
- 108010088535 Pep-1 peptide Proteins 0.000 claims 1
- 229920000776 Poly(Adenosine diphosphate-ribose) polymerase Polymers 0.000 claims 1
- 101800000795 Proadrenomedullin N-20 terminal peptide Proteins 0.000 claims 1
- 241000589516 Pseudomonas Species 0.000 claims 1
- 101710183548 Pyridoxal 5'-phosphate synthase subunit PdxS Proteins 0.000 claims 1
- 102100033975 Ran-binding protein 3 Human genes 0.000 claims 1
- 101710176056 Ran-binding protein 3 Proteins 0.000 claims 1
- 101100247004 Rattus norvegicus Qsox1 gene Proteins 0.000 claims 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims 1
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- 101150086694 SLC22A3 gene Proteins 0.000 claims 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 1
- 101100465559 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PRE7 gene Proteins 0.000 claims 1
- 102100037192 Sal-like protein 4 Human genes 0.000 claims 1
- 108010002687 Survivin Proteins 0.000 claims 1
- 102100036840 T-box transcription factor TBX21 Human genes 0.000 claims 1
- 101150023892 TIMM9 gene Proteins 0.000 claims 1
- 101001023030 Toxoplasma gondii Myosin-D Proteins 0.000 claims 1
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- 241000269370 Xenopus <genus> Species 0.000 claims 1
- 101100445365 Xenopus laevis eomes gene Proteins 0.000 claims 1
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- 125000002091 cationic group Chemical group 0.000 claims 1
- HCQPHKMLKXOJSR-IRCPFGJUSA-N cecropin-a Chemical compound C([C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)NCC(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)NCC(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(N)=O)[C@@H](C)CC)C(C)C)[C@@H](C)CC)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@@H](N)CCCCN)C1=CC=CC=C1 HCQPHKMLKXOJSR-IRCPFGJUSA-N 0.000 claims 1
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- 229940088597 hormone Drugs 0.000 claims 1
- 210000005260 human cell Anatomy 0.000 claims 1
- 206010022000 influenza Diseases 0.000 claims 1
- 101150111214 lin-28 gene Proteins 0.000 claims 1
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- 231100000252 nontoxic Toxicity 0.000 claims 1
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- 239000003053 toxin Substances 0.000 claims 1
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Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2021092230A JP2021121224A (ja) | 2015-04-10 | 2021-06-01 | 標的真核細胞のサイトゾルへのポリペプチドカーゴの形質導入効率を改善するためのポリペプチドベースのシャトル剤、その使用、それと関連する方法及びキット |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562145760P | 2015-04-10 | 2015-04-10 | |
| US62/145,760 | 2015-04-10 | ||
| US201562246892P | 2015-10-27 | 2015-10-27 | |
| US62/246,892 | 2015-10-27 | ||
| PCT/CA2016/050403 WO2016161516A1 (en) | 2015-04-10 | 2016-04-08 | Polypeptide-based shuttle agents for improving the transduction efficiency of polypeptide cargos to the cytosol of target eukaryotic cells, uses thereof, methods and kits relating to same |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021092230A Division JP2021121224A (ja) | 2015-04-10 | 2021-06-01 | 標的真核細胞のサイトゾルへのポリペプチドカーゴの形質導入効率を改善するためのポリペプチドベースのシャトル剤、その使用、それと関連する方法及びキット |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018512891A JP2018512891A (ja) | 2018-05-24 |
| JP2018512891A5 true JP2018512891A5 (https=) | 2019-05-16 |
| JP6956705B2 JP6956705B2 (ja) | 2021-11-02 |
Family
ID=57071637
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018503698A Active JP6956705B2 (ja) | 2015-04-10 | 2016-04-08 | 標的真核細胞のサイトゾルへのポリペプチドカーゴの形質導入効率を改善するためのポリペプチドベースのシャトル剤、その使用、それと関連する方法及びキット |
| JP2021092230A Pending JP2021121224A (ja) | 2015-04-10 | 2021-06-01 | 標的真核細胞のサイトゾルへのポリペプチドカーゴの形質導入効率を改善するためのポリペプチドベースのシャトル剤、その使用、それと関連する方法及びキット |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021092230A Pending JP2021121224A (ja) | 2015-04-10 | 2021-06-01 | 標的真核細胞のサイトゾルへのポリペプチドカーゴの形質導入効率を改善するためのポリペプチドベースのシャトル剤、その使用、それと関連する方法及びキット |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US9738687B2 (https=) |
| EP (1) | EP3280739B1 (https=) |
| JP (2) | JP6956705B2 (https=) |
| KR (2) | KR102697262B1 (https=) |
| CN (1) | CN107636017B (https=) |
| AU (1) | AU2016245347B2 (https=) |
| CA (2) | CA2981716C (https=) |
| ES (1) | ES2864758T3 (https=) |
| IL (1) | IL254919B (https=) |
| SG (1) | SG11201708336YA (https=) |
| WO (1) | WO2016161516A1 (https=) |
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| CA2981716C (en) | 2015-04-10 | 2022-04-12 | Feldan Bio Inc. | Polypeptide-based shuttle agents for improving the transduction efficiency of polypeptide cargos to the cytosol of target eukaryotic cells, uses thereof, methods and kits relating to same |
| US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
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| US10544390B2 (en) | 2016-06-30 | 2020-01-28 | Zymergen Inc. | Methods for generating a bacterial hemoglobin library and uses thereof |
| EP4485466A3 (en) | 2016-08-17 | 2025-04-02 | The Broad Institute Inc. | Novel crispr enzymes and systems |
| WO2018035387A1 (en) | 2016-08-17 | 2018-02-22 | The Broad Institute, Inc. | Novel crispr enzymes and systems |
| US11629170B2 (en) | 2016-10-12 | 2023-04-18 | Feldan Bio Inc. | Rationally-designed synthetic peptide shuttle agents for delivering polypeptide cargos from an extracellular space to the cytosol and/or nucleus of a target eukaryotic cell, uses thereof, methods and kits relating to same |
| CN110291100B (zh) * | 2016-10-12 | 2024-06-11 | 费尔丹生物公司 | 用于将多肽负荷从细胞外空间递送至靶真核细胞的胞质溶胶和/或细胞核的合理设计的合成肽穿梭剂，其用途、与其相关的方法和试剂盒 |
| US9982267B2 (en) | 2016-10-12 | 2018-05-29 | Feldan Bio Inc. | Rationally-designed synthetic peptide shuttle agents for delivering polypeptide cargos from an extracellular space to the cytosol and/or nucleus of a target eukaryotic cell, uses thereof, methods and kits relating to same |
| WO2018129440A1 (en) * | 2017-01-09 | 2018-07-12 | University Of Massachusetts | Complexes for gene deletion and editing |
| KR102746901B1 (ko) | 2017-03-03 | 2024-12-26 | 옵시디안 테라퓨틱스, 인크. | Cd19 조성물 및 면역요법을 위한 방법 |
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| WO2018183850A2 (en) * | 2017-03-31 | 2018-10-04 | Arizona Board Of Regents On Behalf Of The University Of Arizona | Methods, systems, and compositions for inhibiting virulance of a/e family pathogens |
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| US12590288B2 (en) | 2017-04-12 | 2026-03-31 | The Broad Institute, Inc. | Method of treating an inflammatory disease by administering an agent which binds a surface receptor on a tuft cell that induces an ILC class 2 inflammatory response |
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| WO2018213726A1 (en) | 2017-05-18 | 2018-11-22 | The Broad Institute, Inc. | Systems, methods, and compositions for targeted nucleic acid editing |
| WO2018226900A2 (en) | 2017-06-06 | 2018-12-13 | Zymergen Inc. | A htp genomic engineering platform for improving fungal strains |
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| WO2018232195A1 (en) | 2017-06-14 | 2018-12-20 | The Broad Institute, Inc. | Compositions and methods targeting complement component 3 for inhibiting tumor growth |
| KR102761791B1 (ko) | 2017-06-26 | 2025-02-05 | 더 브로드 인스티튜트, 인코퍼레이티드 | 표적화된 핵산 편집을 위한 crispr/cas-아데닌 데아미나아제 기반 조성물, 시스템 및 방법 |
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| AU2018338318B2 (en) | 2017-09-21 | 2022-12-22 | Massachusetts Institute Of Technology | Systems, methods, and compositions for targeted nucleic acid editing |
| WO2019071054A1 (en) | 2017-10-04 | 2019-04-11 | The Broad Institute, Inc. | METHODS AND COMPOSITIONS FOR MODIFYING THE FUNCTION AND STRUCTURE OF BUCKLES AND / OR CHROMATIN DOMAINS |
| WO2019094955A1 (en) | 2017-11-13 | 2019-05-16 | The Broad Institute, Inc. | Methods and compositions for targeting developmental and oncogenic programs in h3k27m gliomas |
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