ES2864758T3 - Agentes lanzadera basados en polipéptidos para mejorar la eficiencia de transducción de cargas polipeptídicas al citosol de células eucariotas objetivo, usos de los mismos, métodos y kits relacionados con los mismos - Google Patents

Agentes lanzadera basados en polipéptidos para mejorar la eficiencia de transducción de cargas polipeptídicas al citosol de células eucariotas objetivo, usos de los mismos, métodos y kits relacionados con los mismos Download PDF

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ES2864758T3
ES2864758T3 ES16775984T ES16775984T ES2864758T3 ES 2864758 T3 ES2864758 T3 ES 2864758T3 ES 16775984 T ES16775984 T ES 16775984T ES 16775984 T ES16775984 T ES 16775984T ES 2864758 T3 ES2864758 T3 ES 2864758T3
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polypeptide
cells
shuttle agent
cpd
domain
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David Guay
Thomas Del'guidice
Jean-Pascal Lepetit-Stoffaes
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Feldan Bio Inc
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Feldan Bio Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/998—Proteins not provided for elsewhere

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
ES16775984T 2015-04-10 2016-04-08 Agentes lanzadera basados en polipéptidos para mejorar la eficiencia de transducción de cargas polipeptídicas al citosol de células eucariotas objetivo, usos de los mismos, métodos y kits relacionados con los mismos Active ES2864758T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562145760P 2015-04-10 2015-04-10
US201562246892P 2015-10-27 2015-10-27
PCT/CA2016/050403 WO2016161516A1 (en) 2015-04-10 2016-04-08 Polypeptide-based shuttle agents for improving the transduction efficiency of polypeptide cargos to the cytosol of target eukaryotic cells, uses thereof, methods and kits relating to same

Publications (1)

Publication Number Publication Date
ES2864758T3 true ES2864758T3 (es) 2021-10-14

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ES16775984T Active ES2864758T3 (es) 2015-04-10 2016-04-08 Agentes lanzadera basados en polipéptidos para mejorar la eficiencia de transducción de cargas polipeptídicas al citosol de células eucariotas objetivo, usos de los mismos, métodos y kits relacionados con los mismos

Country Status (11)

Country Link
US (3) US9738687B2 (https=)
EP (1) EP3280739B1 (https=)
JP (2) JP6956705B2 (https=)
KR (2) KR102697262B1 (https=)
CN (1) CN107636017B (https=)
AU (1) AU2016245347B2 (https=)
CA (2) CA2981716C (https=)
ES (1) ES2864758T3 (https=)
IL (1) IL254919B (https=)
SG (1) SG11201708336YA (https=)
WO (1) WO2016161516A1 (https=)

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