JP2018508198A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2018508198A5 JP2018508198A5 JP2017539228A JP2017539228A JP2018508198A5 JP 2018508198 A5 JP2018508198 A5 JP 2018508198A5 JP 2017539228 A JP2017539228 A JP 2017539228A JP 2017539228 A JP2017539228 A JP 2017539228A JP 2018508198 A5 JP2018508198 A5 JP 2018508198A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- barcode sequence
- separate entities
- multiple copies
- acid barcode
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 150000007523 nucleic acids Chemical class 0.000 claims 6
- 108020004707 nucleic acids Proteins 0.000 claims 6
- 230000003321 amplification Effects 0.000 claims 1
- 230000002255 enzymatic Effects 0.000 claims 1
- 238000010348 incorporation Methods 0.000 claims 1
- 230000002934 lysing Effects 0.000 claims 1
- 238000003199 nucleic acid amplification method Methods 0.000 claims 1
Claims (1)
- 核酸バーコード配列の複数のコピーを、別個の実体中に導入する方法であって、
別個の実体中に複数の核酸標的分子をカプセル封入するステップと、
別個の実体中に、核酸バーコード配列の複数のコピーを含む細胞を導入するステップと、
細胞を溶解して、別個の実体中に核酸バーコード配列の複数のコピーを放出するステップと、
別個の実体を、複数の核酸標的分子またはその増幅産物中への核酸バーコード配列の酵素的組込みに十分な条件に供するステップと
を含む、方法。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022023107A JP2022088360A (ja) | 2015-02-04 | 2022-02-17 | 別個の実体におけるバーコード付加による核酸のシーケンシング |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562112075P | 2015-02-04 | 2015-02-04 | |
| US62/112,075 | 2015-02-04 | ||
| PCT/US2016/016444 WO2016126871A2 (en) | 2015-02-04 | 2016-02-03 | Sequencing of nucleic acids via barcoding in discrete entities |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022023107A Division JP2022088360A (ja) | 2015-02-04 | 2022-02-17 | 別個の実体におけるバーコード付加による核酸のシーケンシング |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2018508198A JP2018508198A (ja) | 2018-03-29 |
| JP2018508198A5 true JP2018508198A5 (ja) | 2019-03-14 |
Family
ID=56564869
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017539228A Pending JP2018508198A (ja) | 2015-02-04 | 2016-02-03 | 別個の実体におけるバーコード付加による核酸のシーケンシング |
| JP2022023107A Pending JP2022088360A (ja) | 2015-02-04 | 2022-02-17 | 別個の実体におけるバーコード付加による核酸のシーケンシング |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022023107A Pending JP2022088360A (ja) | 2015-02-04 | 2022-02-17 | 別個の実体におけるバーコード付加による核酸のシーケンシング |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US11111519B2 (ja) |
| EP (2) | EP4112744A1 (ja) |
| JP (2) | JP2018508198A (ja) |
| CN (2) | CN107530654A (ja) |
| AU (2) | AU2016215304B2 (ja) |
| CA (1) | CA2974306A1 (ja) |
| HK (1) | HK1248169A1 (ja) |
| WO (1) | WO2016126871A2 (ja) |
Families Citing this family (169)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| ES2904816T3 (es) | 2012-02-27 | 2022-04-06 | Becton Dickinson Co | Composiciones para recuento molecular |
| WO2013177560A1 (en) | 2012-05-25 | 2013-11-28 | The Regents Of The University Of California | Microfluidic systems for particle trapping and separation |
| CA2881783A1 (en) | 2012-08-13 | 2014-02-20 | The Regents Of The University Of California | Methods and systems for detecting biological components |
| US9567631B2 (en) | 2012-12-14 | 2017-02-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CA3216609C (en) | 2012-08-14 | 2024-05-14 | 10X Genomics, Inc. | Microcapsule compositions and methods |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CA2900543C (en) | 2013-02-08 | 2023-01-31 | 10X Genomics, Inc. | Partitioning and processing of analytes and other species |
| EP3597772A1 (en) | 2013-04-17 | 2020-01-22 | Agency For Science, Technology And Research | Method for generating extended sequence reads |
| US9879313B2 (en) | 2013-06-25 | 2018-01-30 | Prognosys Biosciences, Inc. | Methods and systems for determining spatial patterns of biological targets in a sample |
| GB2546833B (en) | 2013-08-28 | 2018-04-18 | Cellular Res Inc | Microwell for single cell analysis comprising single cell and single bead oligonucleotide capture labels |
| US9824068B2 (en) | 2013-12-16 | 2017-11-21 | 10X Genomics, Inc. | Methods and apparatus for sorting data |
| WO2015157567A1 (en) | 2014-04-10 | 2015-10-15 | 10X Genomics, Inc. | Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same |
| EP4053292A1 (en) | 2014-06-26 | 2022-09-07 | 10X Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| WO2015200717A2 (en) | 2014-06-27 | 2015-12-30 | The Regents Of The University Of California | Pcr-activated sorting (pas) |
| GB201414177D0 (en) * | 2014-08-11 | 2014-09-24 | Sphere Fluidics Ltd | Droplet Sorting |
| WO2016065056A1 (en) | 2014-10-22 | 2016-04-28 | The Regents Of The University Of California | High definition microdroplet printer |
| CN114807307A (zh) | 2014-10-29 | 2022-07-29 | 10X 基因组学有限公司 | 用于靶核酸测序的方法和组合物 |
| US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
| US10900065B2 (en) | 2014-11-14 | 2021-01-26 | University Of Washington | Methods and kits for labeling cellular molecules |
| BR112017014902A2 (pt) | 2015-01-12 | 2018-03-13 | 10X Genomics Inc | processos e sistemas para a preparação de bibliotecas de sequenciamento de ácido nucleico e bibliotecas preparadas usando os mesmos |
| US11111519B2 (en) | 2015-02-04 | 2021-09-07 | The Regents Of The University Of California | Sequencing of nucleic acids via barcoding in discrete entities |
| US11274343B2 (en) | 2015-02-24 | 2022-03-15 | 10X Genomics, Inc. | Methods and compositions for targeted nucleic acid sequence coverage |
| WO2016137973A1 (en) | 2015-02-24 | 2016-09-01 | 10X Genomics Inc | Partition processing methods and systems |
| EP4119677B1 (en) | 2015-04-10 | 2023-06-28 | Spatial Transcriptomics AB | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| US10982258B2 (en) * | 2015-04-27 | 2021-04-20 | Abvitro Llc | Methods of sequencing, determining, pairing, and validating therapeutic agents and disease specific antigens |
| US20180237836A1 (en) * | 2015-08-17 | 2018-08-23 | The Regents Of The University Of California | Microdroplet-Based Multiple Displacement Amplification (MDA) Methods and Related Compositions |
| US9862941B2 (en) | 2015-10-14 | 2018-01-09 | Pioneer Hi-Bred International, Inc. | Single cell microfluidic device |
| WO2017083375A1 (en) * | 2015-11-10 | 2017-05-18 | Illumina, Inc. | Inertial droplet generation and particle encapsulation |
| US11371094B2 (en) | 2015-11-19 | 2022-06-28 | 10X Genomics, Inc. | Systems and methods for nucleic acid processing using degenerate nucleotides |
| CN115369161A (zh) | 2015-12-04 | 2022-11-22 | 10X 基因组学有限公司 | 用于核酸分析的方法和组合物 |
| EP3414341A4 (en) | 2016-02-11 | 2019-10-09 | 10X Genomics, Inc. | SYSTEMS, METHODS, AND MEDIA FOR ASSEMBLING NOVO OF GENOME SEQUENCE DATA OVERALL |
| WO2017181161A1 (en) | 2016-04-15 | 2017-10-19 | Predicine, Inc. | Systems and methods for detecting genetic alterations |
| WO2018057820A1 (en) | 2016-09-21 | 2018-03-29 | Predicine, Inc. | Systems and methods for combined detection of genetic alterations |
| WO2018183796A1 (en) * | 2017-03-31 | 2018-10-04 | Predicine, Inc. | Systems and methods for predicting and monitoring cancer therapy |
| WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
| US11513076B2 (en) | 2016-06-15 | 2022-11-29 | Ludwig-Maximilians-Universität München | Single molecule detection or quantification using DNA nanotechnology |
| US10830545B2 (en) | 2016-07-12 | 2020-11-10 | Fractal Heatsink Technologies, LLC | System and method for maintaining efficiency of a heat sink |
| JP6853523B2 (ja) * | 2016-07-27 | 2021-03-31 | 国立大学法人京都大学 | ヘリカーゼを用いたｐｃｒ |
| WO2018031691A1 (en) | 2016-08-10 | 2018-02-15 | The Regents Of The University Of California | Combined multiple-displacement amplification and pcr in an emulsion microdroplet |
| CA3032528A1 (en) | 2016-09-15 | 2018-03-22 | Augmenta Bioworks, Inc. | Immune repertoire sequence amplification methods and applications |
| AU2017331459B2 (en) | 2016-09-26 | 2023-04-13 | Becton, Dickinson And Company | Measurement of protein expression using reagents with barcoded oligonucleotide sequences |
| WO2018067792A1 (en) * | 2016-10-07 | 2018-04-12 | President And Fellows Of Harvard College | Sequencing of bacteria or other species |
| EP3525933A1 (en) * | 2016-10-11 | 2019-08-21 | The Regents of the University of California | Systems and methods to encapsulate and preserve organic matter for analysis |
| EP3985122A1 (en) * | 2016-11-10 | 2022-04-20 | Takara Bio USA, Inc. | Methods of producing amplified double stranded deoxyribonucleic acids and compositions and kits for use therein |
| US20190316180A1 (en) * | 2016-11-23 | 2019-10-17 | Universite De Strasbourg | Tandem barcoding of target molecules for their absolute quantification at single-entity resolution |
| CN110462053A (zh) * | 2016-12-21 | 2019-11-15 | 加利福尼亚大学董事会 | 使用基于水凝胶的液滴进行单细胞基因组测序 |
| US10011872B1 (en) * | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| EP4029939B1 (en) | 2017-01-30 | 2023-06-28 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
| US10995333B2 (en) | 2017-02-06 | 2021-05-04 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation |
| CN107083423B (zh) * | 2017-03-27 | 2022-01-28 | 北京极客基因科技有限公司 | 一种药物靶点预测和药物全方面评价方法 |
| WO2018191701A1 (en) * | 2017-04-14 | 2018-10-18 | The Broad Institute, Inc. | High-throughput screens for exploring biological functions of microscale biological systems |
| EP4215616A1 (en) | 2017-05-18 | 2023-07-26 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| US10544413B2 (en) | 2017-05-18 | 2020-01-28 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| SG11201901822QA (en) | 2017-05-26 | 2019-03-28 | 10X Genomics Inc | Single cell analysis of transposase accessible chromatin |
| US20180340169A1 (en) | 2017-05-26 | 2018-11-29 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| AU2018273999A1 (en) * | 2017-05-26 | 2019-12-05 | Abvitro Llc | High-throughput polynucleotide library sequencing and transcriptome analysis |
| WO2018227210A1 (en) | 2017-06-09 | 2018-12-13 | The Regents Of The University Of California | High-efficiency encapsulation in droplets based on hydrodynamic vortices control |
| US11517901B2 (en) | 2017-06-09 | 2022-12-06 | The Regents Of The University Of California | High-efficiency particle encapsulation in droplets with particle spacing and downstream droplet sorting |
| WO2018236918A1 (en) | 2017-06-20 | 2018-12-27 | Bio-Rad Laboratories, Inc. | MDA USING A BALL OLIGONUCLEOTIDE |
| TWI637834B (zh) * | 2017-07-11 | 2018-10-11 | 台灣奈米碳素股份有限公司 | 微流道裝置的製造方法及其結構 |
| US11031312B2 (en) | 2017-07-17 | 2021-06-08 | Fractal Heatsink Technologies, LLC | Multi-fractal heatsink system and method |
| CN111511912A (zh) * | 2017-08-10 | 2020-08-07 | 梅塔生物科技公司 | 标记来自单个细胞的核酸分子以进行定相测序 |
| US10610865B2 (en) | 2017-08-22 | 2020-04-07 | 10X Genomics, Inc. | Droplet forming devices and system with differential surface properties |
| US11680283B2 (en) | 2017-09-22 | 2023-06-20 | University Of Washington | In situ combinatorial labeling of cellular molecules |
| US11639933B2 (en) * | 2017-09-27 | 2023-05-02 | Bio-Rad Laboratories, Inc. | Digital affinity linkage assay |
| US20200261879A1 (en) | 2017-09-29 | 2020-08-20 | The Regents Of The University Of California | Method of generating monodisperse emulsions |
| US10837047B2 (en) | 2017-10-04 | 2020-11-17 | 10X Genomics, Inc. | Compositions, methods, and systems for bead formation using improved polymers |
| WO2019075409A1 (en) | 2017-10-12 | 2019-04-18 | The Regents Of The University Of California | ISOLATION AND IDENTIFICATION WITHOUT MICROFLUIDIC LABEL OF CELLS USING FLUORESCENCE LIFE IMAGING (FLIM) |
| US10501739B2 (en) | 2017-10-18 | 2019-12-10 | Mission Bio, Inc. | Method, systems and apparatus for single cell analysis |
| WO2019079640A1 (en) * | 2017-10-18 | 2019-04-25 | Mission Bio, Inc. | METHOD, SYSTEMS, AND DEVICE FOR HIGH-EFFICIENCY UNICELLULAR DNA SEQUENCING OF DROPLET MICROFLUIDS |
| WO2019079787A1 (en) | 2017-10-20 | 2019-04-25 | The Regents Of The University Of California | MICROFLUIDIC SYSTEMS AND METHODS FOR CELL TRANSFECTION VIA LIPOFLEX |
| US11499127B2 (en) | 2017-10-20 | 2022-11-15 | The Regents Of The University Of California | Multi-layered microfluidic systems for in vitro large-scale perfused capillary networks |
| WO2019083852A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING |
| WO2019084043A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS |
| EP3700672B1 (en) | 2017-10-27 | 2022-12-28 | 10X Genomics, Inc. | Methods for sample preparation and analysis |
| CN111051523B (zh) | 2017-11-15 | 2024-03-19 | 10X基因组学有限公司 | 功能化凝胶珠 |
| US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| WO2019104337A1 (en) | 2017-11-27 | 2019-05-31 | The Trustees Of Columbia University In The City Of New York | Rna printing and sequencing devices, methods, and systems |
| WO2019108851A1 (en) | 2017-11-30 | 2019-06-06 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation and analysis |
| WO2019113235A1 (en) * | 2017-12-06 | 2019-06-13 | 10X Genomics, Inc. | Methods and systems for processing nucleic acid molecules |
| CN111699388A (zh) * | 2017-12-12 | 2020-09-22 | 10X基因组学有限公司 | 用于单细胞处理的系统和方法 |
| CN111556900A (zh) * | 2017-12-22 | 2020-08-18 | 赛默飞世尔科技波罗的海封闭股份公司 | 包括胺的聚合酶链反应组合物 |
| EP3752832A1 (en) | 2018-02-12 | 2020-12-23 | 10X Genomics, Inc. | Methods characterizing multiple analytes from individual cells or cell populations |
| US11639928B2 (en) | 2018-02-22 | 2023-05-02 | 10X Genomics, Inc. | Methods and systems for characterizing analytes from individual cells or cell populations |
| WO2019169028A1 (en) * | 2018-02-28 | 2019-09-06 | 10X Genomics, Inc. | Transcriptome sequencing through random ligation |
| WO2019173460A1 (en) * | 2018-03-07 | 2019-09-12 | Wisconsin Alumni Research Foundation | High throughput nucleic acid profiling of single cells |
| SG11202009889VA (en) | 2018-04-06 | 2020-11-27 | 10X Genomics Inc | Systems and methods for quality control in single cell processing |
| WO2019224219A1 (en) * | 2018-05-22 | 2019-11-28 | Universite De Strasbourg | Absolute quantification of target molecules at single-entity resolution using tandem barcoding |
| US11932899B2 (en) | 2018-06-07 | 2024-03-19 | 10X Genomics, Inc. | Methods and systems for characterizing nucleic acid molecules |
| US11703427B2 (en) | 2018-06-25 | 2023-07-18 | 10X Genomics, Inc. | Methods and systems for cell and bead processing |
| US20210261953A1 (en) * | 2018-07-03 | 2021-08-26 | Chan Zuckerberg Biohub, Inc. | Method to perform high-throughput single cell genomic and phenotypic analyses |
| WO2020010258A1 (en) * | 2018-07-05 | 2020-01-09 | Accuragen Holdings Limited | Compositions and methods for digital polymerase chain reaction |
| US20200032335A1 (en) | 2018-07-27 | 2020-01-30 | 10X Genomics, Inc. | Systems and methods for metabolome analysis |
| US20200048626A1 (en) * | 2018-08-07 | 2020-02-13 | Augmenta Bioworks, Inc. | Methods for Making Gel Beads and Core and Shell Beads with a Cell |
| US20210346888A1 (en) | 2018-08-17 | 2021-11-11 | The Regents Of The University Of California | Monodispersed Particle-Triggered Droplet Formation from Stable Jets |
| US20200056223A1 (en) * | 2018-08-20 | 2020-02-20 | 10X Genomics, Inc. | Compositions and methods for cellular processing |
| JP2022511398A (ja) | 2018-10-01 | 2022-01-31 | ベクトン・ディキンソン・アンド・カンパニー | ５’転写物配列の決定 |
| WO2020077017A2 (en) | 2018-10-10 | 2020-04-16 | Augmenta Bioworks, Inc. | Methods for isolating immune binding proteins |
| US11608524B1 (en) * | 2018-10-25 | 2023-03-21 | Wisconsin Alumni Research Foundation | Methods of analyzing cells |
| US11459607B1 (en) | 2018-12-10 | 2022-10-04 | 10X Genomics, Inc. | Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes |
| EP3894593A2 (en) | 2018-12-13 | 2021-10-20 | DNA Script | Direct oligonucleotide synthesis on cells and biomolecules |
| CN111378557B (zh) | 2018-12-26 | 2023-06-06 | 财团法人工业技术研究院 | 用于产生液珠的管状结构及液珠产生方法 |
| US11845983B1 (en) | 2019-01-09 | 2023-12-19 | 10X Genomics, Inc. | Methods and systems for multiplexing of droplet based assays |
| CN113490751A (zh) * | 2019-01-22 | 2021-10-08 | 使命生物公司 | 核酸检测和引物设计方法 |
| US11467153B2 (en) | 2019-02-12 | 2022-10-11 | 10X Genomics, Inc. | Methods for processing nucleic acid molecules |
| SG11202108788TA (en) | 2019-02-12 | 2021-09-29 | 10X Genomics Inc | Methods for processing nucleic acid molecules |
| US11851683B1 (en) | 2019-02-12 | 2023-12-26 | 10X Genomics, Inc. | Methods and systems for selective analysis of cellular samples |
| US11655499B1 (en) | 2019-02-25 | 2023-05-23 | 10X Genomics, Inc. | Detection of sequence elements in nucleic acid molecules |
| SG11202111242PA (en) * | 2019-03-11 | 2021-11-29 | 10X Genomics Inc | Systems and methods for processing optically tagged beads |
| WO2020183591A1 (en) * | 2019-03-11 | 2020-09-17 | Enplas Corporation | Fluid handling method, and fluid handling device and fluid handling system used for the method |
| EP3947724A4 (en) * | 2019-04-02 | 2023-06-14 | Mission Bio, Inc. | METHODS AND SYSTEMS FOR CHARACTERIZING TUMORS AND IDENTIFYING TUMOR HETEROGENY |
| EP3947674A4 (en) * | 2019-04-02 | 2023-06-07 | Mission Bio, Inc. | METHODS, SYSTEMS AND APPARATUS FOR NUCLEIC ACID DETECTION |
| EP3973074A4 (en) | 2019-05-22 | 2023-09-06 | Mission Bio, Inc. | METHOD AND DEVICE FOR SIMULTANEOUS TARGETED SEQUENCING OF DNA, RNA AND PROTEIN |
| WO2020252387A2 (en) * | 2019-06-12 | 2020-12-17 | Ultima Genomics, Inc. | Methods for accurate base calling using molecular barcodes |
| US20230212556A1 (en) * | 2019-06-27 | 2023-07-06 | Cell Microsystems, Inc. | Systems and methods for associating single cell imaging with rna transcriptomics |
| US11667954B2 (en) | 2019-07-01 | 2023-06-06 | Mission Bio, Inc. | Method and apparatus to normalize quantitative readouts in single-cell experiments |
| CN113891961A (zh) * | 2019-07-12 | 2022-01-04 | 深圳华大生命科学研究院 | 一种全基因组全流程微流控自动化建库方法和装置 |
| US11939622B2 (en) | 2019-07-22 | 2024-03-26 | Becton, Dickinson And Company | Single cell chromatin immunoprecipitation sequencing assay |
| WO2021022085A2 (en) * | 2019-07-31 | 2021-02-04 | Bioskryb, Inc. | Single cell analysis |
| US20220282326A1 (en) * | 2019-08-05 | 2022-09-08 | Mission Bio, Inc. | Method and Apparatus for Single-Cell Analysis for Determining a Cell Trajectory |
| EP4025909A4 (en) * | 2019-09-04 | 2023-10-18 | Vanderbilt University | METHODS OF IDENTIFYING ANTIBODY ANTIGEN BINDING SPECIFICITY |
| WO2021067286A1 (en) * | 2019-09-30 | 2021-04-08 | Shield Diagnostics Corp. | Devices for determining a target nucleic acid profile and methods of use thereof |
| JP2022550596A (ja) * | 2019-10-05 | 2022-12-02 | ミッション バイオ インコーポレイテッド | シングルセルで同時に検出されるコピー数変動及び単一ヌクレオチド変動のための方法、システム、及び装置 |
| US11919000B2 (en) | 2019-10-10 | 2024-03-05 | 1859, Inc. | Methods and systems for microfluidic screening |
| US20220381781A1 (en) * | 2019-10-16 | 2022-12-01 | Georgia Tech Research Corporation | Methods for ultrasensitive detection of protein and cellular biomarkers |
| CN110777048A (zh) * | 2019-11-01 | 2020-02-11 | 上海市第六人民医院 | 一种高通量单外泌体分离装置 |
| CN114729350A (zh) | 2019-11-08 | 2022-07-08 | 贝克顿迪金森公司 | 使用随机引发获得用于免疫组库测序的全长v(d)j信息 |
| US20210205811A1 (en) * | 2019-11-19 | 2021-07-08 | 10X Genomics, Inc. | Methods and devices for sorting droplets and particles |
| WO2021128035A1 (zh) * | 2019-12-25 | 2021-07-01 | 苏州绘真生物科技有限公司 | 高通量单细胞转录组测序方法及试剂盒 |
| CN115244184A (zh) | 2020-01-13 | 2022-10-25 | 贝克顿迪金森公司 | 用于定量蛋白和rna的方法和组合物 |
| US11512337B2 (en) | 2020-01-13 | 2022-11-29 | Fluent Biosciences Inc. | Emulsion based drug screening |
| WO2021146184A1 (en) | 2020-01-13 | 2021-07-22 | Fluent Biosciences Inc. | Single cell sequencing |
| WO2021146166A1 (en) | 2020-01-13 | 2021-07-22 | Fluent Biosciences Inc. | Methods and systems for single cell gene profiling |
| US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
| EP4095256A4 (en) * | 2020-01-23 | 2023-03-15 | DGI Tech (Qing Dao) Co., Limited | DROPLET MICROFLUIDIC-BASED SINGLE CELL SEQUENCING AND APPLICATIONS |
| US11835462B2 (en) | 2020-02-11 | 2023-12-05 | 10X Genomics, Inc. | Methods and compositions for partitioning a biological sample |
| US11926863B1 (en) | 2020-02-27 | 2024-03-12 | 10X Genomics, Inc. | Solid state single cell method for analyzing fixed biological cells |
| WO2021188500A1 (en) | 2020-03-16 | 2021-09-23 | Fluent Biosciences Inc. | Multi-omic analysis in monodisperse droplets |
| CN111454942A (zh) * | 2020-03-16 | 2020-07-28 | 张晓鲁 | 一种同一样品的转录组和基因组同时建立测序文库的构建方法 |
| WO2021222512A1 (en) * | 2020-04-30 | 2021-11-04 | Dimensiongen | Devices and methods for macromolecular manipulation |
| GB202007059D0 (en) * | 2020-05-13 | 2020-06-24 | Univ Oxford Innovation Ltd | Polynucleotide arrays |
| US11851700B1 (en) | 2020-05-13 | 2023-12-26 | 10X Genomics, Inc. | Methods, kits, and compositions for processing extracellular molecules |
| EP4153775A1 (en) | 2020-05-22 | 2023-03-29 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| CA3185619A1 (en) * | 2020-05-29 | 2021-12-02 | Riken | Method for treating cell population and method for analyzing genes included in cell population |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| GB202012730D0 (en) * | 2020-08-14 | 2020-09-30 | Cambridge Entpr Ltd | Modular microfluidic devices, systems and methodds for total RNA analyses |
| WO2022099208A1 (en) * | 2020-11-09 | 2022-05-12 | The General Hospital Corporation | High throughput microfluidics for analysis of immune cell activation |
| EP4244379A1 (en) * | 2020-11-13 | 2023-09-20 | 10X Genomics, Inc. | Nano-partitions (encapsulated nucleic acid processing enzymes) for cell-lysis and multiple reactions in partition-based assays |
| US11739443B2 (en) | 2020-11-20 | 2023-08-29 | Becton, Dickinson And Company | Profiling of highly expressed and lowly expressed proteins |
| US20220243264A1 (en) * | 2020-11-23 | 2022-08-04 | The Broad Institute, Inc. | Systems and methods for amplifying rna |
| CN113322254B (zh) * | 2021-01-06 | 2022-05-20 | 南京诺唯赞生物科技股份有限公司 | 多靶点蛋白质-dna相互作用的研究方法和工具 |
| CN114836838A (zh) * | 2021-02-01 | 2022-08-02 | 南方医科大学 | 一种中通量单细胞拷贝数文库构建的方法及其应用 |
| WO2022182682A1 (en) | 2021-02-23 | 2022-09-01 | 10X Genomics, Inc. | Probe-based analysis of nucleic acids and proteins |
| WO2023044307A1 (en) * | 2021-09-14 | 2023-03-23 | Becton, Dickinson And Company | Full length single cell rna sequencing |
| WO2023154816A1 (en) * | 2022-02-09 | 2023-08-17 | Mission Bio, Inc. | Systems and methods of detecting merged droplets in single cell sequencing |
| DK202200230A1 (en) * | 2022-03-21 | 2023-12-11 | Samplix Aps | Targeted enrichment of large dna molecules for long-read sequencing using facs or microfluidic partitioning |
| WO2023212223A1 (en) * | 2022-04-28 | 2023-11-02 | BioSkryb Genomics, Inc. | Single cell multiomics |
| WO2024097393A1 (en) * | 2022-11-04 | 2024-05-10 | Fluid Discovery | Systems, compositions, and methods for single cell analysis |
Family Cites Families (119)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU5733994A (en) * | 1992-11-30 | 1994-06-22 | Wellcome Foundation Limited, The | Sequential targeting of tumor sites with oligonucleotide conjugates of antibody and complementary oligonucleotide conjugates of chelated radionuclide |
| US20020068357A1 (en) | 1995-09-28 | 2002-06-06 | Mathies Richard A. | Miniaturized integrated nucleic acid processing and analysis device and method |
| US6143496A (en) | 1997-04-17 | 2000-11-07 | Cytonix Corporation | Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly |
| US6319507B1 (en) | 1997-05-02 | 2001-11-20 | Kobo Products, Inc. | Agar gel bead composition and method |
| ES2283936T3 (es) | 1997-07-07 | 2007-11-01 | Medical Research Council | Procedimiento de clasificacion in vitro. |
| US6436677B1 (en) | 2000-03-02 | 2002-08-20 | Promega Corporation | Method of reverse transcription |
| US7041208B2 (en) | 2001-12-11 | 2006-05-09 | Sau Lan Tang Staats | Microfluidic devices and methods for two-dimensional separations |
| JP2006507921A (ja) | 2002-06-28 | 2006-03-09 | プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ | 流体分散のための方法および装置 |
| JP4480715B2 (ja) | 2003-01-29 | 2010-06-16 | ４５４ コーポレーション | 二重末端シーケンシング |
| US10533998B2 (en) * | 2008-07-18 | 2020-01-14 | Bio-Rad Laboratories, Inc. | Enzyme quantification |
| US7041481B2 (en) | 2003-03-14 | 2006-05-09 | The Regents Of The University Of California | Chemical amplification based on fluid partitioning |
| DE10339452A1 (de) * | 2003-08-22 | 2005-03-17 | Institut für Physikalische Hochtechnologie e.V. | Vorrichtung und Verfahren zur Strukturierung von Flüssigkeiten |
| EP2662136A3 (en) | 2003-08-27 | 2013-12-25 | President and Fellows of Harvard College | Method for handling and mixing droplets |
| US20050069938A1 (en) | 2003-09-26 | 2005-03-31 | Youxiang Wang | Amplification of polynucleotides by rolling circle amplification |
| EP1691792A4 (en) | 2003-11-24 | 2008-05-28 | Yeda Res & Dev | COMPOSITIONS AND METHODS FOR IN VITRO / I SORTING OF MOLECULAR AND CELLULAR BANKS |
| KR100647282B1 (ko) | 2003-12-24 | 2006-11-17 | 삼성전자주식회사 | ＤＮＡ 마이크로어레이 스폿팅(Ｓｐｏｔｉｎｇ) 장치 및이를 이용한 스폿팅 방법 |
| US20060201868A1 (en) | 2005-03-11 | 2006-09-14 | Simmons Blake A | Methods and devices for high-throughput dielectrophoretic concentration |
| WO2007024798A2 (en) | 2005-08-22 | 2007-03-01 | Applera Corporation | Apparatus, system, and method using immiscible-fluid-discrete-volumes |
| US8383059B2 (en) | 2005-09-30 | 2013-02-26 | University Of Utah Research Foundation | Microfluidic interface for highly parallel addressing of sensing arrays |
| US9183349B2 (en) | 2005-12-16 | 2015-11-10 | Nextbio | Sequence-centric scientific information management |
| EP1984738A2 (en) | 2006-01-11 | 2008-10-29 | Raindance Technologies, Inc. | Microfluidic devices and methods of use in the formation and control of nanoreactors |
| US7998708B2 (en) | 2006-03-24 | 2011-08-16 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
| EP2530167A1 (en) | 2006-05-11 | 2012-12-05 | Raindance Technologies, Inc. | Microfluidic Devices |
| WO2007140015A2 (en) * | 2006-05-26 | 2007-12-06 | Althea Technologies, Inc | Biochemical analysis of partitioned cells |
| ES2397971T3 (es) | 2006-06-02 | 2013-03-12 | Pfizer Products Incorporated | Ensayo de células tumorales circulantes |
| DK2415872T3 (en) | 2006-12-14 | 2016-08-22 | Dow Agrosciences Llc | Optimized non-canonical zinc finger proteins |
| US8338166B2 (en) | 2007-01-04 | 2012-12-25 | Lawrence Livermore National Security, Llc | Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture |
| WO2008109176A2 (en) | 2007-03-07 | 2008-09-12 | President And Fellows Of Harvard College | Assays and other reactions involving droplets |
| US8454906B2 (en) | 2007-07-24 | 2013-06-04 | The Regents Of The University Of California | Microfabricated droplet generator for single molecule/cell genetic analysis in engineered monodispersed emulsions |
| US8268564B2 (en) | 2007-09-26 | 2012-09-18 | President And Fellows Of Harvard College | Methods and applications for stitched DNA barcodes |
| GB0720202D0 (en) * | 2007-10-16 | 2007-11-28 | Cambridge Entpr Ltd | Microfluidic systems |
| WO2009092106A1 (en) | 2008-01-18 | 2009-07-23 | The Regents Of The University Of California | Accurate and rapid micromixer for integrated microfluidic devices |
| US8030095B2 (en) | 2008-03-04 | 2011-10-04 | Crystal Bioscience Inc. | Gel microdrop composition and method of using the same |
| US9409177B2 (en) | 2008-03-21 | 2016-08-09 | Lawrence Livermore National Security, Llc | Chip-based device for parallel sorting, amplification, detection, and identification of nucleic acid subsequences |
| WO2009137415A2 (en) | 2008-05-03 | 2009-11-12 | Advanced Liquid Logic, Inc. | Reagent and sample preparation, loading, and storage |
| WO2011120024A1 (en) | 2010-03-25 | 2011-09-29 | Quantalife, Inc. | Droplet generation for droplet-based assays |
| US9156010B2 (en) | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
| US8709762B2 (en) | 2010-03-02 | 2014-04-29 | Bio-Rad Laboratories, Inc. | System for hot-start amplification via a multiple emulsion |
| US20130084572A1 (en) | 2011-09-30 | 2013-04-04 | Quantalife, Inc. | Calibrations and controls for droplet-based assays |
| US9132394B2 (en) | 2008-09-23 | 2015-09-15 | Bio-Rad Laboratories, Inc. | System for detection of spaced droplets |
| JP5457222B2 (ja) | 2009-02-25 | 2014-04-02 | エフ．ホフマン−ラ ロシュ アーゲー | 小型化ハイスループット核酸分析 |
| DK2977455T3 (da) | 2009-06-15 | 2020-07-13 | Complete Genomics Inc | Fremgangsmåde til langfragmentaflæsnings-sekventering |
| CN102483424B (zh) | 2009-06-26 | 2014-11-05 | 哈佛学院院长等 | 用于流体操作的方法以及微流体装置 |
| EP2473618B1 (en) | 2009-09-02 | 2015-03-04 | Bio-Rad Laboratories, Inc. | System for mixing fluids by coalescence of multiple emulsions |
| US9625454B2 (en) * | 2009-09-04 | 2017-04-18 | The Research Foundation For The State University Of New York | Rapid and continuous analyte processing in droplet microfluidic devices |
| US8746285B2 (en) | 2009-09-04 | 2014-06-10 | Auburn University | Programmable fluidic droplet generation |
| US9376713B2 (en) | 2009-09-23 | 2016-06-28 | The Board Of Trustees Of The University Of Illinois | Label free detection of nucleic acid amplification |
| US9890408B2 (en) | 2009-10-15 | 2018-02-13 | Ibis Biosciences, Inc. | Multiple displacement amplification |
| US9315857B2 (en) | 2009-12-15 | 2016-04-19 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse label-tags |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US20120270739A1 (en) | 2010-01-19 | 2012-10-25 | Verinata Health, Inc. | Method for sample analysis of aneuploidies in maternal samples |
| AU2013203624B2 (en) | 2010-02-09 | 2015-06-25 | General Electric Company | Isothermal amplification of nucleic acid using a mixture of randomized primers and specific primers |
| US9366632B2 (en) | 2010-02-12 | 2016-06-14 | Raindance Technologies, Inc. | Digital analyte analysis |
| CA2767114A1 (en) | 2010-03-25 | 2011-09-29 | Bio-Rad Laboratories, Inc. | Droplet transport system for detection |
| JP5573335B2 (ja) | 2010-04-28 | 2014-08-20 | 株式会社島津製作所 | 磁性体粒子操作デバイス及び磁性体粒子操作方法 |
| WO2011154042A1 (en) | 2010-06-10 | 2011-12-15 | Albert-Ludwigs-Universität Freiburg | Apparatus and method for dispensing cells or particles confined in a free flying droplet |
| US9650629B2 (en) | 2010-07-07 | 2017-05-16 | Roche Molecular Systems, Inc. | Clonal pre-amplification in emulsion |
| WO2012011091A2 (en) | 2010-07-22 | 2012-01-26 | Gencell Biosystems Limited | Composite liquid cells |
| EP2608878A4 (en) | 2010-08-23 | 2017-11-15 | President and Fellows of Harvard College | Acoustic waves in microfluidics |
| EP3447155A1 (en) | 2010-09-30 | 2019-02-27 | Raindance Technologies, Inc. | Sandwich assays in droplets |
| EP2436444A1 (en) | 2010-10-01 | 2012-04-04 | Centre National de la Recherche Scientifique (C.N.R.S.) | Microfluidic device for production and collection of droplets of a fluid |
| ES2731460T3 (es) | 2010-10-08 | 2019-11-15 | Harvard College | Alto rendimiento de células individuales con código de barra |
| US20120258881A1 (en) | 2010-11-22 | 2012-10-11 | The University Of Chicago | Methods and/or Use of Oligonucleotide Conjugates for Assays and Microscopy/Imaging Detections |
| ES2615733T3 (es) | 2010-12-16 | 2017-06-08 | Gigagen, Inc. | Métodos para el análisis masivo paralelo de ácidos nucleicos en células individuales |
| US20130295587A1 (en) | 2011-01-24 | 2013-11-07 | Ge Healthcare Bio-Sciences Ab | Method of coupling binding agents to a substrate surface |
| US8765455B2 (en) | 2011-01-27 | 2014-07-01 | Lawrence Livermore National Security, Llc | Chip-based droplet sorting |
| WO2012106385A2 (en) * | 2011-01-31 | 2012-08-09 | Apprise Bio, Inc. | Methods of identifying multiple epitopes in cells |
| EP2670894B1 (en) | 2011-02-02 | 2017-11-29 | University Of Washington Through Its Center For Commercialization | Massively parallel continguity mapping |
| EP3859011A1 (en) * | 2011-02-11 | 2021-08-04 | Bio-Rad Laboratories, Inc. | Methods for forming mixed droplets |
| US9150852B2 (en) * | 2011-02-18 | 2015-10-06 | Raindance Technologies, Inc. | Compositions and methods for molecular labeling |
| EP2697397B1 (en) | 2011-04-15 | 2017-04-05 | The Johns Hopkins University | Safe sequencing system |
| EP3789498A1 (en) | 2011-04-25 | 2021-03-10 | Bio-rad Laboratories, Inc. | Methods for nucleic acid analysis |
| WO2012162267A2 (en) | 2011-05-20 | 2012-11-29 | Fluidigm Corporation | Nucleic acid encoding reactions |
| EP2714254B1 (en) | 2011-05-23 | 2017-09-06 | President and Fellows of Harvard College | Control of emulsions, including multiple emulsions |
| US8841071B2 (en) | 2011-06-02 | 2014-09-23 | Raindance Technologies, Inc. | Sample multiplexing |
| US8658430B2 (en) | 2011-07-20 | 2014-02-25 | Raindance Technologies, Inc. | Manipulating droplet size |
| US20130189700A1 (en) | 2011-07-25 | 2013-07-25 | Bio-Rad Laboratories, Inc. | Breakage of an emulsion containing nucleic acid |
| JP6161607B2 (ja) | 2011-07-26 | 2017-07-12 | ベリナタ ヘルス インコーポレイテッド | サンプルにおける異なる異数性の有無を決定する方法 |
| US20130032235A1 (en) | 2011-08-02 | 2013-02-07 | Teledyne Dalsa Semiconductor, Inc. | Integrated microfluidic check valve and device including such a check valve |
| US9469874B2 (en) * | 2011-10-18 | 2016-10-18 | The Regents Of The University Of California | Long-range barcode labeling-sequencing |
| US9483374B2 (en) | 2011-12-21 | 2016-11-01 | Intel Corporation | PSMI using at-speed scan capture |
| KR20140122751A (ko) | 2012-02-08 | 2014-10-20 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 유체 파열을 사용한 액적 형성 |
| WO2013126741A1 (en) | 2012-02-24 | 2013-08-29 | Raindance Technologies, Inc. | Labeling and sample preparation for sequencing |
| US11177020B2 (en) | 2012-02-27 | 2021-11-16 | The University Of North Carolina At Chapel Hill | Methods and uses for molecular tags |
| EP2823064B1 (en) | 2012-03-05 | 2019-02-06 | President and Fellows of Harvard College | Methods for epigenetic sequencing |
| US20150132754A1 (en) * | 2012-05-14 | 2015-05-14 | Cb Biotechnologies, Inc. | Method for increasing accuracy in quantitative detection of polynucleotides |
| CA2881783A1 (en) | 2012-08-13 | 2014-02-20 | The Regents Of The University Of California | Methods and systems for detecting biological components |
| US9567631B2 (en) | 2012-12-14 | 2017-02-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US20140378345A1 (en) * | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| CA3216609C (en) | 2012-08-14 | 2024-05-14 | 10X Genomics, Inc. | Microcapsule compositions and methods |
| US20140378349A1 (en) | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| WO2014039912A1 (en) | 2012-09-07 | 2014-03-13 | Bio-Rad Laboratories, Inc. | Compositions, systems and methods for droplet formation, spacing and detection |
| WO2014047556A1 (en) | 2012-09-21 | 2014-03-27 | The Broad Institute, Inc. | Compositions and methods for long insert, paired end libraries of nucleic acids in emulsion droplets |
| EP2925449A2 (en) | 2012-11-27 | 2015-10-07 | Gencell Biosystems Limited | Handling liquid samples |
| WO2014108323A1 (en) | 2013-01-10 | 2014-07-17 | Eth Zurich | Method and apparatus for depositing droplets onto a substrate |
| EP2964776A4 (en) | 2013-03-04 | 2017-04-19 | Verax Biomedical, Inc. | Multi-analyte assay |
| WO2014153071A1 (en) | 2013-03-14 | 2014-09-25 | The Broad Institute, Inc. | Methods for quantitating dna using digital multiple displacement amplification |
| US9752179B2 (en) * | 2013-03-14 | 2017-09-05 | Cold Spring Harbor Laboratory | Trans-splicing transcriptome profiling |
| US9816088B2 (en) * | 2013-03-15 | 2017-11-14 | Abvitro Llc | Single cell bar-coding for antibody discovery |
| KR102168053B1 (ko) | 2013-03-15 | 2020-10-20 | 더 리전트 오브 더 유니버시티 오브 캘리포니아 | 고속 온 디맨드 미세유체 액적 생성 및 조작 |
| US9328382B2 (en) * | 2013-03-15 | 2016-05-03 | Complete Genomics, Inc. | Multiple tagging of individual long DNA fragments |
| GB2546833B (en) | 2013-08-28 | 2018-04-18 | Cellular Res Inc | Microwell for single cell analysis comprising single cell and single bead oligonucleotide capture labels |
| US10947587B2 (en) | 2013-11-05 | 2021-03-16 | The Regents Of The University Of California | Single-cell forensic short tandem repeat typing within microfluidic droplets |
| EP3105326A4 (en) | 2014-02-10 | 2018-01-10 | Gencell Biosystems Limited | Composite liquid cell (clc) mediated nucleic acid library preparation device, and methods for using the same |
| CN113215221A (zh) | 2014-04-08 | 2021-08-06 | 华盛顿大学商业中心 | 用于使用多分散小滴执行数字检定的方法和设备 |
| US20150298091A1 (en) | 2014-04-21 | 2015-10-22 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
| CN106574925B (zh) | 2014-05-23 | 2019-07-26 | 萤火虫生物股份有限公司 | 用于生物测定的底物介导的反应器 |
| JP6666268B2 (ja) | 2014-06-11 | 2020-03-13 | サンプリクス アンパーツゼルスカブ | 液滴ソートによるヌクレオチド配列排除富化（ｎｅｅｄｌｓ） |
| EP4053292A1 (en) * | 2014-06-26 | 2022-09-07 | 10X Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| WO2015200717A2 (en) | 2014-06-27 | 2015-12-30 | The Regents Of The University Of California | Pcr-activated sorting (pas) |
| WO2016064755A2 (en) | 2014-10-20 | 2016-04-28 | The Regents Of The University Of Califronia | Rapid modulation of droplet composition with membrane microvalves |
| WO2016065056A1 (en) | 2014-10-22 | 2016-04-28 | The Regents Of The University Of California | High definition microdroplet printer |
| US11111519B2 (en) | 2015-02-04 | 2021-09-07 | The Regents Of The University Of California | Sequencing of nucleic acids via barcoding in discrete entities |
| US20170022538A1 (en) | 2015-02-04 | 2017-01-26 | The Regents Of The University Of California | Multiple-emulsion nucleic acid amplification |
| US20180237836A1 (en) | 2015-08-17 | 2018-08-23 | The Regents Of The University Of California | Microdroplet-Based Multiple Displacement Amplification (MDA) Methods and Related Compositions |
| WO2017184707A1 (en) | 2016-04-19 | 2017-10-26 | President And Fellows Of Harvard College | Immobilization-based systems and methods for genetic analysis and other applications |
| WO2018031691A1 (en) | 2016-08-10 | 2018-02-15 | The Regents Of The University Of California | Combined multiple-displacement amplification and pcr in an emulsion microdroplet |
| CN110462053A (zh) | 2016-12-21 | 2019-11-15 | 加利福尼亚大学董事会 | 使用基于水凝胶的液滴进行单细胞基因组测序 |
| EP4212629A1 (en) | 2017-11-17 | 2023-07-19 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
-
2016
- 2016-02-03 US US15/015,015 patent/US11111519B2/en active Active
- 2016-02-03 JP JP2017539228A patent/JP2018508198A/ja active Pending
- 2016-02-03 AU AU2016215304A patent/AU2016215304B2/en active Active
- 2016-02-03 EP EP22190412.1A patent/EP4112744A1/en active Pending
- 2016-02-03 CN CN201680020884.4A patent/CN107530654A/zh active Pending
- 2016-02-03 WO PCT/US2016/016444 patent/WO2016126871A2/en active Application Filing
- 2016-02-03 CN CN202210828875.5A patent/CN115011670A/zh active Pending
- 2016-02-03 EP EP16747229.9A patent/EP3253479B1/en active Active
- 2016-02-03 CA CA2974306A patent/CA2974306A1/en active Pending
-
2018
- 2018-03-29 US US15/940,850 patent/US11732287B2/en active Active
- 2018-06-13 HK HK18107667.7A patent/HK1248169A1/zh unknown
-
2022
- 2022-02-17 JP JP2022023107A patent/JP2022088360A/ja active Pending
- 2022-04-27 AU AU2022202780A patent/AU2022202780A1/en active Pending
-
2023
- 2023-06-22 US US18/212,868 patent/US20240150811A1/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2018508198A5 (ja) | ||
| NZ738689A (en) | Engineered crispr-cas9 compositions and methods of use | |
| JP2019515674A5 (ja) | ||
| WO2016057951A3 (en) | Crispr oligonucleotides and gene editing | |
| WO2015200717A3 (en) | Pcr-activated sorting (pas) | |
| EP4026905C0 (en) | METHODS OF BARCODING NUCLEIC ACID MOLECULES FROM INDIVIDUAL CELLS OR POPULATIONS OF CELLS | |
| SG11201803646YA (en) | Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells | |
| MY189819A (en) | Genetically modified cells and uses thereof | |
| WO2015089511A3 (en) | Modified nucleic acid molecules and uses thereof | |
| WO2016126871A3 (en) | Sequencing of nucleic acids via barcoding in discrete entities | |
| BR112016019267B8 (pt) | Método para construir um perfil de dna, biblioteca de ácido nucléico, método para construir uma biblioteca de ácido nucléico, pluralidade de iniciadores e kit | |
| WO2016184955A3 (de) | Verfahren und konstrukte zur gezielten nukleinsäure editierung in pflanzen | |
| JP2013005803A5 (ja) | ||
| JP2018515133A5 (ja) | ||
| BR112015004864A2 (pt) | enriquecimento de classificação de células ativadas por fluorescência (facs) para gerar plantas | |
| MX2018004263A (es) | Edición genómica multiplexada. | |
| WO2015196128A3 (en) | Alternative nucleic acid molecules and uses thereof | |
| WO2015196130A3 (en) | Alternative nucleic acid molecules and uses thereof | |
| WO2015160895A3 (en) | Modified transposases for improved insertion sequence bias and increased dna input tolerance | |
| AR092479A1 (es) | Plataforma de integracion de transgenes modificados por ingenieria genetica (etip) para reconocimiento de genes y apilamiento de rasgos | |
| WO2016033251A3 (en) | Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation | |
| ECSP14028704A (es) | Métodos y composiciones para la modificación de adn objetivo dirigida por arn y para la modulación de la transcripción dirigida por arn | |
| JP2016537982A5 (ja) | ||
| BR112017027527A2 (pt) | ?método para a produção de feromônios de mariposas em levedura | |
| JP2018518943A5 (ja) |