JP2017538446A5 - - Google Patents
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- Publication number
- JP2017538446A5 JP2017538446A5 JP2017547931A JP2017547931A JP2017538446A5 JP 2017538446 A5 JP2017538446 A5 JP 2017538446A5 JP 2017547931 A JP2017547931 A JP 2017547931A JP 2017547931 A JP2017547931 A JP 2017547931A JP 2017538446 A5 JP2017538446 A5 JP 2017538446A5
- Authority
- JP
- Japan
- Prior art keywords
- recombinant protein
- perfusion
- mammalian host
- bioreactor
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 26
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims 14
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims 14
- 230000010412 perfusion Effects 0.000 claims 7
- 239000006143 cell culture medium Substances 0.000 claims 5
- 150000004676 glycans Chemical class 0.000 claims 4
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims 2
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims 2
- 229910052802 copper Inorganic materials 0.000 claims 2
- 239000010949 copper Substances 0.000 claims 2
- 238000009472 formulation Methods 0.000 claims 2
- 229910052748 manganese Inorganic materials 0.000 claims 2
- 239000011572 manganese Substances 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 241000699802 Cricetulus griseus Species 0.000 claims 1
- 102000004127 Cytokines Human genes 0.000 claims 1
- 108090000695 Cytokines Proteins 0.000 claims 1
- 102000003886 Glycoproteins Human genes 0.000 claims 1
- 108090000288 Glycoproteins Proteins 0.000 claims 1
- 230000003698 anagen phase Effects 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 239000012636 effector Substances 0.000 claims 1
- 238000003306 harvesting Methods 0.000 claims 1
- 210000001672 ovary Anatomy 0.000 claims 1
- 230000000630 rising effect Effects 0.000 claims 1
- 230000007704 transition Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462085759P | 2014-12-01 | 2014-12-01 | |
| US62/085,759 | 2014-12-01 | ||
| PCT/US2015/063271 WO2016089919A1 (en) | 2014-12-01 | 2015-12-01 | Process for manipulating the level of glycan content of a glycoprotein |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020080270A Division JP7066775B2 (ja) | 2014-12-01 | 2020-04-30 | 糖タンパク質のグリカン含量のレベルを操作するためのプロセス |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017538446A JP2017538446A (ja) | 2017-12-28 |
| JP2017538446A5 true JP2017538446A5 (OSRAM) | 2019-01-17 |
| JP6698681B2 JP6698681B2 (ja) | 2020-05-27 |
Family
ID=55024253
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017547931A Active JP6698681B2 (ja) | 2014-12-01 | 2015-12-01 | 糖タンパク質のグリカン含量のレベルを操作するためのプロセス |
| JP2020080270A Active JP7066775B2 (ja) | 2014-12-01 | 2020-04-30 | 糖タンパク質のグリカン含量のレベルを操作するためのプロセス |
| JP2022072126A Pending JP2022101669A (ja) | 2014-12-01 | 2022-04-26 | 糖タンパク質のグリカン含量のレベルを操作するためのプロセス |
| JP2024063188A Pending JP2024095777A (ja) | 2014-12-01 | 2024-04-10 | 糖タンパク質のグリカン含量のレベルを操作するためのプロセス |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020080270A Active JP7066775B2 (ja) | 2014-12-01 | 2020-04-30 | 糖タンパク質のグリカン含量のレベルを操作するためのプロセス |
| JP2022072126A Pending JP2022101669A (ja) | 2014-12-01 | 2022-04-26 | 糖タンパク質のグリカン含量のレベルを操作するためのプロセス |
| JP2024063188A Pending JP2024095777A (ja) | 2014-12-01 | 2024-04-10 | 糖タンパク質のグリカン含量のレベルを操作するためのプロセス |
Country Status (22)
| Country | Link |
|---|---|
| US (5) | US10167492B2 (OSRAM) |
| EP (2) | EP3227454B1 (OSRAM) |
| JP (4) | JP6698681B2 (OSRAM) |
| KR (1) | KR102623965B1 (OSRAM) |
| CN (1) | CN107109455B (OSRAM) |
| AU (5) | AU2015355087C1 (OSRAM) |
| CA (1) | CA2969225C (OSRAM) |
| CL (1) | CL2017001369A1 (OSRAM) |
| CY (1) | CY1123247T1 (OSRAM) |
| DK (1) | DK3227454T3 (OSRAM) |
| EA (2) | EA202091598A3 (OSRAM) |
| ES (1) | ES2784503T3 (OSRAM) |
| HU (1) | HUE049201T2 (OSRAM) |
| IL (2) | IL276165B (OSRAM) |
| LT (1) | LT3227454T (OSRAM) |
| MX (1) | MX385409B (OSRAM) |
| PL (1) | PL3227454T3 (OSRAM) |
| PT (1) | PT3227454T (OSRAM) |
| SG (2) | SG10202002458PA (OSRAM) |
| SI (1) | SI3227454T1 (OSRAM) |
| WO (1) | WO2016089919A1 (OSRAM) |
| ZA (2) | ZA201703729B (OSRAM) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3227454B1 (en) | 2014-12-01 | 2020-01-29 | Amgen Inc. | Process for manipulating the level of glycan content of a glycoprotein |
| WO2017117218A1 (en) | 2015-12-30 | 2017-07-06 | Momenta Pharmaceuticals, Inc. | Methods related to biologics |
| CN110418846A (zh) * | 2017-03-14 | 2019-11-05 | 美国安进公司 | 细胞培养物中产生的抗体的总去岩藻糖基化糖型的控制 |
| EP3601586A1 (en) * | 2017-03-31 | 2020-02-05 | Boehringer Ingelheim International GmbH | Perfusion medium |
| CN111373028B (zh) * | 2017-11-30 | 2022-07-05 | 正大天晴药业集团南京顺欣制药有限公司 | 蛋白质的生产方法 |
| SG11202009216YA (en) * | 2018-03-26 | 2020-10-29 | Amgen Inc | Total afucosylated glycoforms of antibodies produced in cell culture |
| JP2021524745A (ja) * | 2018-05-24 | 2021-09-16 | アレス トレーディング ソシエテ アノニム | 糖タンパク質組成物の非フコシル化レベルを制御する方法 |
| MX2020013036A (es) | 2018-06-05 | 2021-02-26 | Amgen Inc | Modulacion de la fagocitosis celular dependiente de anticuerpos. |
| US20220033511A1 (en) | 2018-09-11 | 2022-02-03 | Amgen Inc. | Methods of modulating antibody-dependent cell-mediated cytotoxicity |
| HU231514B1 (hu) | 2018-11-07 | 2024-07-28 | Richter Gedeon Nyrt. | Sejttenyészetben előállított rekombináns glikoprotein glikozilációs mintázatának megváltoztatására szolgáló módszer |
| CA3117447C (en) | 2018-11-13 | 2024-06-04 | Janssen Biotech, Inc. | Control of trace metals during production of anti-cd38 antibodies |
| MX2021008030A (es) * | 2018-12-31 | 2021-08-05 | Momenta Pharmaceuticals Inc | Metodos para producir ustekinumab. |
| US20220098279A1 (en) * | 2019-01-30 | 2022-03-31 | Amgen Inc. | Aflibercept attributes and methods of characterizing and modifying thereof |
| BR112021022202A2 (pt) | 2019-05-06 | 2021-12-28 | Amgen Inc | Modulação de funções efetoras de anticorpo |
| JP2020188737A (ja) * | 2019-05-23 | 2020-11-26 | 東ソー株式会社 | 抗体依存性細胞傷害活性が向上した抗体の製造方法 |
| KR20220069982A (ko) | 2019-09-26 | 2022-05-27 | 암젠 인크 | 항체 조성물의 생산 방법 |
| US20230287335A1 (en) * | 2020-07-30 | 2023-09-14 | Amgen Inc. | Cell culture media and methods of making and using the same |
| CN116391043A (zh) * | 2020-08-14 | 2023-07-04 | 百时美施贵宝公司 | 蛋白质的制造方法 |
| AU2021360897A1 (en) | 2020-10-15 | 2023-05-25 | Amgen Inc. | Relative unpaired glycans in antibody production methods |
| TW202317614A (zh) | 2021-06-07 | 2023-05-01 | 美商安進公司 | 使用岩藻糖苷酶控制糖基化蛋白的去岩藻糖基化水平 |
| WO2023161885A1 (en) * | 2022-02-25 | 2023-08-31 | Kashiv Biosciences, Llc | A process for improving polypeptide expression in mammalian cell culture |
| WO2024220916A1 (en) | 2023-04-20 | 2024-10-24 | Amgen Inc. | Methods of determining relative unpaired glycan content |
| WO2025036924A1 (en) * | 2023-08-16 | 2025-02-20 | Glaxosmithkline Intellectual Property Limited | Method of controlling antibody glycosylation profile |
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-
2015
- 2015-12-01 EP EP15816598.5A patent/EP3227454B1/en not_active Revoked
- 2015-12-01 DK DK15816598.5T patent/DK3227454T3/da active
- 2015-12-01 LT LTEP15816598.5T patent/LT3227454T/lt unknown
- 2015-12-01 CA CA2969225A patent/CA2969225C/en active Active
- 2015-12-01 SG SG10202002458PA patent/SG10202002458PA/en unknown
- 2015-12-01 CN CN201580071982.6A patent/CN107109455B/zh active Active
- 2015-12-01 HU HUE15816598A patent/HUE049201T2/hu unknown
- 2015-12-01 JP JP2017547931A patent/JP6698681B2/ja active Active
- 2015-12-01 EA EA202091598A patent/EA202091598A3/ru unknown
- 2015-12-01 PL PL15816598T patent/PL3227454T3/pl unknown
- 2015-12-01 US US15/529,950 patent/US10167492B2/en active Active
- 2015-12-01 AU AU2015355087A patent/AU2015355087C1/en active Active
- 2015-12-01 EA EA201791223A patent/EA036178B1/ru not_active IP Right Cessation
- 2015-12-01 MX MX2017006997A patent/MX385409B/es unknown
- 2015-12-01 KR KR1020177016893A patent/KR102623965B1/ko active Active
- 2015-12-01 IL IL276165A patent/IL276165B/en unknown
- 2015-12-01 WO PCT/US2015/063271 patent/WO2016089919A1/en not_active Ceased
- 2015-12-01 SI SI201531140T patent/SI3227454T1/sl unknown
- 2015-12-01 PT PT158165985T patent/PT3227454T/pt unknown
- 2015-12-01 ES ES15816598T patent/ES2784503T3/es active Active
- 2015-12-01 EP EP20154019.2A patent/EP3680344A1/en active Pending
- 2015-12-01 SG SG11201704351WA patent/SG11201704351WA/en unknown
-
2017
- 2017-05-29 CL CL2017001369A patent/CL2017001369A1/es unknown
- 2017-05-29 IL IL252569A patent/IL252569B/en active IP Right Grant
- 2017-05-31 ZA ZA2017/03729A patent/ZA201703729B/en unknown
-
2018
- 2018-11-14 US US16/191,352 patent/US20190085370A1/en not_active Abandoned
-
2020
- 2020-02-05 US US16/783,117 patent/US10822630B2/en active Active
- 2020-03-31 AU AU2020202302A patent/AU2020202302A1/en not_active Abandoned
- 2020-04-07 CY CY20201100325T patent/CY1123247T1/el unknown
- 2020-04-30 JP JP2020080270A patent/JP7066775B2/ja active Active
- 2020-09-29 ZA ZA2020/06002A patent/ZA202006002B/en unknown
- 2020-10-02 US US17/062,199 patent/US20210017565A1/en not_active Abandoned
-
2022
- 2022-01-28 AU AU2022200586A patent/AU2022200586A1/en not_active Abandoned
- 2022-04-26 JP JP2022072126A patent/JP2022101669A/ja active Pending
-
2023
- 2023-01-18 US US18/156,025 patent/US20230159974A1/en not_active Abandoned
-
2024
- 2024-04-10 JP JP2024063188A patent/JP2024095777A/ja active Pending
- 2024-06-24 AU AU2024204313A patent/AU2024204313A1/en not_active Abandoned
-
2025
- 2025-09-01 AU AU2025223948A patent/AU2025223948A1/en active Pending
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