JP2017536104A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2017536104A5 JP2017536104A5 JP2017520506A JP2017520506A JP2017536104A5 JP 2017536104 A5 JP2017536104 A5 JP 2017536104A5 JP 2017520506 A JP2017520506 A JP 2017520506A JP 2017520506 A JP2017520506 A JP 2017520506A JP 2017536104 A5 JP2017536104 A5 JP 2017536104A5
- Authority
- JP
- Japan
- Prior art keywords
- protein
- fused
- ret
- gpcr
- fragment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000004169 proteins and genes Human genes 0.000 claims 90
- 108090000623 proteins and genes Proteins 0.000 claims 90
- 238000002165 resonance energy transfer Methods 0.000 claims 77
- 239000012634 fragment Substances 0.000 claims 33
- 102000003688 G-Protein-Coupled Receptors Human genes 0.000 claims 24
- 108090000045 G-Protein-Coupled Receptors Proteins 0.000 claims 24
- 229940125633 GPCR agonist Drugs 0.000 claims 16
- 210000000170 cell membrane Anatomy 0.000 claims 16
- 230000008685 targeting Effects 0.000 claims 14
- 239000003795 chemical substances by application Substances 0.000 claims 12
- 238000000034 method Methods 0.000 claims 6
- 239000003814 drug Substances 0.000 claims 5
- 229940079593 drug Drugs 0.000 claims 5
- 239000012190 activator Substances 0.000 claims 4
- 239000003112 inhibitor Substances 0.000 claims 4
- 229940125499 GPCR antagonist Drugs 0.000 claims 3
- 108091006027 G proteins Proteins 0.000 claims 2
- 102000030782 GTP binding Human genes 0.000 claims 2
- 108091000058 GTP-Binding Proteins 0.000 claims 2
- 108010047357 Luminescent Proteins Proteins 0.000 claims 2
- 102000006830 Luminescent Proteins Human genes 0.000 claims 2
- 108091006047 fluorescent proteins Proteins 0.000 claims 2
- 102000034287 fluorescent proteins Human genes 0.000 claims 2
- 230000004927 fusion Effects 0.000 claims 2
- 230000004952 protein activity Effects 0.000 claims 2
- 102100021738 Beta-adrenergic receptor kinase 1 Human genes 0.000 claims 1
- 102100037281 Beta-adrenergic receptor kinase 2 Human genes 0.000 claims 1
- 101000751445 Homo sapiens Beta-adrenergic receptor kinase 1 Proteins 0.000 claims 1
- 101000806653 Homo sapiens Beta-adrenergic receptor kinase 2 Proteins 0.000 claims 1
- 108060001084 Luciferase Proteins 0.000 claims 1
- 239000005089 Luciferase Substances 0.000 claims 1
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 150000001413 amino acids Chemical class 0.000 claims 1
- 239000005557 antagonist Substances 0.000 claims 1
- 210000004027 cell Anatomy 0.000 claims 1
- 239000012528 membrane Substances 0.000 claims 1
- 108010010909 olfactory G protein subunit alpha olf Proteins 0.000 claims 1
- 230000013823 prenylation Effects 0.000 claims 1
- 210000001938 protoplast Anatomy 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462063622P | 2014-10-14 | 2014-10-14 | |
| US62/063,622 | 2014-10-14 | ||
| PCT/CA2015/051032 WO2016058094A1 (en) | 2014-10-14 | 2015-10-14 | BIOSENSOR BASED ON GβΥ-INTERACTING PROTEINS TO MONITOR G-PROTEIN ACTIVATION |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017536104A JP2017536104A (ja) | 2017-12-07 |
| JP2017536104A5 true JP2017536104A5 (enExample) | 2018-10-18 |
| JP6932636B2 JP6932636B2 (ja) | 2021-09-08 |
Family
ID=55745919
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017520506A Active JP6932636B2 (ja) | 2014-10-14 | 2015-10-14 | Ｇタンパク質活性化を監視するためのＧβγ相互作用タンパク質に基づいたバイオセンサー |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US10877036B2 (enExample) |
| EP (1) | EP3207052B1 (enExample) |
| JP (1) | JP6932636B2 (enExample) |
| CN (1) | CN107074926B (enExample) |
| AU (1) | AU2015333543B2 (enExample) |
| CA (1) | CA2963579C (enExample) |
| DK (1) | DK3207052T3 (enExample) |
| ES (1) | ES2832331T3 (enExample) |
| IL (1) | IL250949B (enExample) |
| NZ (1) | NZ729735A (enExample) |
| PL (1) | PL3207052T3 (enExample) |
| SG (1) | SG11201701895VA (enExample) |
| WO (1) | WO2016058094A1 (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019062744A1 (zh) * | 2017-09-27 | 2019-04-04 | 北京大学 | 融合多肽 |
| KR20200071724A (ko) * | 2017-10-18 | 2020-06-19 | 유니버시떼 드 몬트리얼 | G-단백질 활성화의 평가를 위한 시스템 및 방법 |
| WO2019076262A1 (zh) * | 2017-10-18 | 2019-04-25 | 深圳华大生命科学研究院 | 检测目标分子化合物的生物感应器及包含该生物感应器的系统 |
| US12360111B2 (en) * | 2018-12-04 | 2025-07-15 | The University Of North Carolina At Chapel Hill | G protein-coupled receptor screening systems |
| FR3092172B1 (fr) * | 2019-01-30 | 2021-02-12 | Cisbio Bioassays | Méthode pour mesurer la modulation de l’activation d’un récepteur couplé à une protéine G avec des analogues du GTP |
| CN110981943B (zh) * | 2019-12-02 | 2021-08-03 | 清华大学 | 多肽及其在制备药物中的用途和药物 |
| KR102778534B1 (ko) | 2022-10-07 | 2025-03-07 | 성균관대학교산학협력단 | G 단백질 알파 서브유닛의 알파-헬리컬 도메인의 실시간 구조 변화 확인 방법 및 시스템 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2003221878A1 (en) * | 2002-04-12 | 2003-10-27 | Schering Corporation | G-protein coupled receptor ligands and methods |
| US20050042720A1 (en) | 2003-04-24 | 2005-02-24 | 7Tm Pharma A/S | Novel promiscuous G alpha protein mutants and their use |
| US7488583B2 (en) | 2003-09-25 | 2009-02-10 | Odyssey Thera, Inc. | Fragment complementation assays for G-protein-coupled receptors and their signaling pathways |
| WO2005121755A1 (en) * | 2004-06-14 | 2005-12-22 | Commonwealth Scientific And Industrial Research Organisation | Cell free g-protein coupled receptor and ligand assay |
| GB0822259D0 (en) * | 2008-12-05 | 2009-01-14 | Ge Healthcare Uk Ltd | Methods for testing binding of a ligand to a G protein- coupled receptor |
| SG172321A1 (en) * | 2009-01-29 | 2011-07-28 | Commw Scient Ind Res Org | Measuring g protein coupled receptor activation |
| GB0905419D0 (en) | 2009-03-30 | 2009-05-13 | Ge Healthcare Uk Ltd | Methods for testing ligand binding to G protein-coupled receptors |
| WO2012063832A1 (ja) | 2010-11-11 | 2012-05-18 | 宇部興産株式会社 | 非水電解液用容器、容器入り非水電解液、及び非水電解液の保存方法 |
| US20140031525A1 (en) * | 2011-01-21 | 2014-01-30 | Heptares Therapeutics Limited | Mutant g-protein coupled receptor proteins and methods for producing them |
| WO2015095973A1 (en) | 2013-12-23 | 2015-07-02 | Université de Montréal | Systems and methods for the monitoring of protein complex formation in cells |
-
2015
- 2015-10-14 US US15/518,888 patent/US10877036B2/en active Active
- 2015-10-14 DK DK15851395.2T patent/DK3207052T3/da active
- 2015-10-14 CA CA2963579A patent/CA2963579C/en active Active
- 2015-10-14 NZ NZ729735A patent/NZ729735A/en unknown
- 2015-10-14 SG SG11201701895VA patent/SG11201701895VA/en unknown
- 2015-10-14 CN CN201580055471.5A patent/CN107074926B/zh active Active
- 2015-10-14 EP EP15851395.2A patent/EP3207052B1/en active Active
- 2015-10-14 AU AU2015333543A patent/AU2015333543B2/en active Active
- 2015-10-14 PL PL15851395T patent/PL3207052T3/pl unknown
- 2015-10-14 WO PCT/CA2015/051032 patent/WO2016058094A1/en not_active Ceased
- 2015-10-14 JP JP2017520506A patent/JP6932636B2/ja active Active
- 2015-10-14 ES ES15851395T patent/ES2832331T3/es active Active
-
2017
- 2017-03-05 IL IL250949A patent/IL250949B/en unknown
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2017536104A5 (enExample) | ||
| Mazur et al. | The proapoptotic influenza A virus protein PB1‐F2 regulates viral polymerase activity by interaction with the PB1 protein | |
| Tawaratsumida et al. | Quantitative proteomic analysis of the influenza A virus nonstructural proteins NS1 and NS2 during natural cell infection identifies PACT as an NS1 target protein and antiviral host factor | |
| Friedlová et al. | IFITM protein regulation and functions: Far beyond the fight against viruses | |
| Ozgur et al. | Human Pat1b connects deadenylation with mRNA decapping and controls the assembly of processing bodies | |
| Blyth et al. | Duck interferon-inducible transmembrane protein 3 mediates restriction of influenza viruses | |
| Pal et al. | Smoothened determines β-arrestin–mediated removal of the G protein–coupled receptor Gpr161 from the primary cilium | |
| Lürick et al. | Multivalent Rab interactions determine tether-mediated membrane fusion | |
| Berscheminski et al. | Sp100 isoform-specific regulation of human adenovirus 5 gene expression | |
| Moisy et al. | HMGB1 protein binds to influenza virus nucleoprotein and promotes viral replication | |
| JP2017534851A5 (enExample) | ||
| Trinh et al. | Avidity binding of human adenovirus serotypes 3 and 7 to the membrane cofactor CD46 triggers infection | |
| Lu et al. | Mediation of opioid analgesia by a truncated 6-transmembrane GPCR | |
| Maar et al. | Cysteines in the stalk of the Nipah virus G glycoprotein are located in a distinct subdomain critical for fusion activation | |
| Schierhorn et al. | Influenza A virus virulence depends on two amino acids in the N-terminal domain of its NS1 protein to facilitate inhibition of the RNA-dependent protein kinase PKR | |
| Emmott et al. | Quantitative proteomics using SILAC coupled to LC− MS/MS reveals changes in the nucleolar proteome in influenza A virus-infected cells | |
| Maroui et al. | Requirement of PML SUMO interacting motif for RNF4-or arsenic trioxide-induced degradation of nuclear PML isoforms | |
| JP2010522541A5 (enExample) | ||
| JP2016516735A5 (enExample) | ||
| Gao et al. | Interaction of NS2 with AIMP2 facilitates the switch from ubiquitination to SUMOylation of M1 in influenza A virus-infected cells | |
| JP2011188853A5 (enExample) | ||
| JP2014239695A5 (enExample) | ||
| JP2017533181A5 (enExample) | ||
| Taylor et al. | Inhibition of TNF‐α‐induced activation of NF‐κB by hantavirus nucleocapsid proteins | |
| Gao et al. | Characteristics of nucleocytoplasmic transport of H1N1 influenza A virus nuclear export protein |