SG11201701895VA - BIOSENSOR BASED ON GβΥ-INTERACTING PROTEINS TO MONITOR G-PROTEIN ACTIVATION - Google Patents

BIOSENSOR BASED ON GβΥ-INTERACTING PROTEINS TO MONITOR G-PROTEIN ACTIVATION

Info

Publication number
SG11201701895VA
SG11201701895VA SG11201701895VA SG11201701895VA SG11201701895VA SG 11201701895V A SG11201701895V A SG 11201701895VA SG 11201701895V A SG11201701895V A SG 11201701895VA SG 11201701895V A SG11201701895V A SG 11201701895VA SG 11201701895V A SG11201701895V A SG 11201701895VA
Authority
SG
Singapore
Prior art keywords
gβυ
monitor
interacting proteins
protein activation
biosensor based
Prior art date
Application number
SG11201701895VA
Inventor
Michel Bouvier
Gouill Christian Le
Mireille Hogue
Viktoriya Lukasheva
Original Assignee
Univ Montreal
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Montreal filed Critical Univ Montreal
Publication of SG11201701895VA publication Critical patent/SG11201701895VA/en

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4722G-proteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/723G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0069Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y113/00Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/60Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G01N2333/47Assays involving proteins of known structure or function as defined in the subgroups
    • G01N2333/4701Details
    • G01N2333/4719G-proteins
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00Screening for compounds of potential therapeutic value
    • G01N2500/02Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
SG11201701895VA 2014-10-14 2015-10-14 BIOSENSOR BASED ON GβΥ-INTERACTING PROTEINS TO MONITOR G-PROTEIN ACTIVATION SG11201701895VA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462063622P 2014-10-14 2014-10-14
PCT/CA2015/051032 WO2016058094A1 (en) 2014-10-14 2015-10-14 BIOSENSOR BASED ON GβΥ-INTERACTING PROTEINS TO MONITOR G-PROTEIN ACTIVATION

Publications (1)

Publication Number Publication Date
SG11201701895VA true SG11201701895VA (en) 2017-04-27

Family

ID=55745919

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201701895VA SG11201701895VA (en) 2014-10-14 2015-10-14 BIOSENSOR BASED ON GβΥ-INTERACTING PROTEINS TO MONITOR G-PROTEIN ACTIVATION

Country Status (13)

Country Link
US (1) US10877036B2 (en)
EP (1) EP3207052B1 (en)
JP (1) JP6932636B2 (en)
CN (1) CN107074926B (en)
AU (1) AU2015333543B2 (en)
CA (1) CA2963579C (en)
DK (1) DK3207052T3 (en)
ES (1) ES2832331T3 (en)
IL (1) IL250949B (en)
NZ (1) NZ729735A (en)
PL (1) PL3207052T3 (en)
SG (1) SG11201701895VA (en)
WO (1) WO2016058094A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019062744A1 (en) * 2017-09-27 2019-04-04 北京大学 Fusion polypeptide
KR20200071724A (en) * 2017-10-18 2020-06-19 유니버시떼 드 몬트리얼 Systems and methods for the evaluation of G-protein activation
WO2019076262A1 (en) * 2017-10-18 2019-04-25 深圳华大生命科学研究院 Biosensor for detecting target molecular compound, and system comprising biosensor
US20220002357A1 (en) * 2018-12-04 2022-01-06 The University Of North Carolina At Chapel Hill G protein-coupled receptor screening systems
FR3092172B1 (en) 2019-01-30 2021-02-12 Cisbio Bioassays Method for measuring the modulation of activation of a G protein coupled receptor with GTP analogues
CN110981943B (en) * 2019-12-02 2021-08-03 清华大学 Polypeptide, application thereof in preparation of medicine and medicine
KR20240049746A (en) 2022-10-07 2024-04-17 성균관대학교산학협력단 Method for Real-time Structural Change Designation of Alpha-helical Domain of G protein Alpha Subunit and the System Using Thereof

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1324047C (en) * 2002-04-12 2007-07-04 先灵公司 G-protein coupled receptor ligands and methods
US20050042720A1 (en) 2003-04-24 2005-02-24 7Tm Pharma A/S Novel promiscuous G alpha protein mutants and their use
US7488583B2 (en) 2003-09-25 2009-02-10 Odyssey Thera, Inc. Fragment complementation assays for G-protein-coupled receptors and their signaling pathways
WO2005121755A1 (en) * 2004-06-14 2005-12-22 Commonwealth Scientific And Industrial Research Organisation Cell free g-protein coupled receptor and ligand assay
GB0822259D0 (en) * 2008-12-05 2009-01-14 Ge Healthcare Uk Ltd Methods for testing binding of a ligand to a G protein- coupled receptor
CN102439444B (en) * 2009-01-29 2014-10-22 联邦科学技术研究组织 Measuring g protein coupled receptor activation
GB0905419D0 (en) 2009-03-30 2009-05-13 Ge Healthcare Uk Ltd Methods for testing ligand binding to G protein-coupled receptors
JP5170350B2 (en) 2010-11-11 2013-03-27 宇部興産株式会社 Non-aqueous electrolyte container, non-aqueous electrolyte in container, and storage method of non-aqueous electrolyte
WO2012098413A1 (en) * 2011-01-21 2012-07-26 Heptares Therapeutics Limited Mutant g-protein coupled receptor proteins and methods for producing them
WO2015095973A1 (en) 2013-12-23 2015-07-02 Université de Montréal Systems and methods for the monitoring of protein complex formation in cells

Also Published As

Publication number Publication date
WO2016058094A1 (en) 2016-04-21
CA2963579C (en) 2023-10-31
EP3207052B1 (en) 2020-09-30
NZ729735A (en) 2023-05-26
PL3207052T3 (en) 2021-02-08
CN107074926A (en) 2017-08-18
AU2015333543B2 (en) 2021-03-04
EP3207052A4 (en) 2018-04-25
CA2963579A1 (en) 2016-04-21
IL250949A0 (en) 2017-04-30
AU2015333543A1 (en) 2017-03-23
CN107074926B (en) 2021-09-21
DK3207052T3 (en) 2020-11-16
US10877036B2 (en) 2020-12-29
US20170234870A1 (en) 2017-08-17
IL250949B (en) 2021-07-29
JP6932636B2 (en) 2021-09-08
JP2017536104A (en) 2017-12-07
ES2832331T3 (en) 2021-06-10
EP3207052A1 (en) 2017-08-23

Similar Documents

Publication Publication Date Title
HK1209303A1 (en) Analyte sensing system
EP3143401A4 (en) Improved assay methods
SG10202111841TA (en) Nanopore-based analysis of protein characteristics
BR112017006622A2 (en) boronic acid derivatives
IL250949B (en) Biosensor based on g-beta-gamma-interacting proteins to monitor g-protein activation
HK1231352A1 (en) Ultrasonic debrider probe
GB201403820D0 (en) Assay
SG11201606516RA (en) Probe unit
GB201400646D0 (en) Biosensing electrodes
EP3242129A4 (en) Electrochemical biosensor
GB2548733B (en) Nucleic acid analysis device
SG11201702178TA (en) Probe unit
GB2533151B (en) Improved probe
GB201415674D0 (en) Nucleic acid analysis
GB2522210B (en) Engine probe
EP3199615A4 (en) Nucleic acid analysis device
GB201414492D0 (en) Matric potential sensor
GB2533166B (en) Sensor molecule
GB201517390D0 (en) Protein variant analysis
PL3192448T3 (en) Elasticity detection probe
GB201421816D0 (en) Analyte Measurement
GB201416211D0 (en) Sequencing techniques
GB201418633D0 (en) Improved assay
GB201409785D0 (en) Sequencing Techniques
GB201412797D0 (en) Proteins